##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031778_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51475 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76104905293832 34.0 31.0 34.0 30.0 34.0 2 31.81723166585721 34.0 31.0 34.0 30.0 34.0 3 31.822166100048566 34.0 31.0 34.0 29.0 34.0 4 35.02494414764449 37.0 35.0 37.0 33.0 37.0 5 35.0878678970374 37.0 35.0 37.0 33.0 37.0 6 35.25431762991744 37.0 37.0 37.0 33.0 37.0 7 35.286508013598834 37.0 37.0 37.0 33.0 37.0 8 35.289888295288975 37.0 37.0 37.0 33.0 37.0 9 37.03335599805731 39.0 39.0 39.0 34.0 39.0 10-11 37.062331228751816 39.0 39.0 39.0 34.5 39.0 12-13 37.03327829043225 39.0 39.0 39.0 34.5 39.0 14-15 38.442564351627 41.0 39.0 41.0 34.5 41.0 16-17 38.32403108305002 41.0 39.0 41.0 34.0 41.0 18-19 38.296940262263234 41.0 39.0 41.0 34.0 41.0 20-21 38.24975230694512 41.0 39.0 41.0 34.0 41.0 22-23 38.08156386595435 41.0 38.5 41.0 34.0 41.0 24-25 37.99432734337057 41.0 38.0 41.0 34.0 41.0 26-27 37.739057795046136 40.5 37.5 41.0 33.5 41.0 28-29 37.51736765420107 40.0 36.5 41.0 33.0 41.0 30-31 37.17919378339 40.0 35.0 41.0 33.0 41.0 32-33 36.862758620689654 40.0 35.0 41.0 32.5 41.0 34-35 36.58987858183585 40.0 35.0 41.0 31.0 41.0 36-37 36.35624089363769 40.0 35.0 41.0 30.0 41.0 38-39 36.06143759106362 39.5 35.0 41.0 29.0 41.0 40-41 35.84513841670714 39.0 35.0 41.0 27.5 41.0 42-43 35.52068965517242 39.0 35.0 41.0 24.5 41.0 44-45 35.33593006313745 39.5 35.0 41.0 21.0 41.0 46-47 34.93638659543468 40.0 35.0 41.0 16.5 41.0 48-49 34.56685769791161 39.5 35.0 41.0 13.0 41.0 50-51 33.53806702282661 38.0 34.0 40.0 10.5 40.5 52-53 33.50753763963089 38.5 34.5 39.5 10.0 40.5 54-55 34.08019426906265 39.0 35.0 41.0 10.0 41.0 56-57 34.03895094706168 39.0 35.0 41.0 10.0 41.0 58-59 33.898047595920346 39.0 35.0 41.0 10.0 41.0 60-61 33.67197668771248 39.0 35.0 41.0 8.5 41.0 62-63 33.37484215638659 38.0 34.5 41.0 8.0 41.0 64-65 33.0365711510442 37.0 34.0 41.0 8.0 41.0 66-67 32.76162214667315 37.0 34.0 40.5 8.0 41.0 68-69 32.414881010199124 36.0 33.0 40.0 7.5 41.0 70-71 32.015288975230696 36.0 33.0 39.0 7.0 41.0 72-73 31.5869159786304 35.0 32.5 39.0 7.0 41.0 74-75 31.18000971345313 35.0 32.0 38.5 7.0 40.5 76-77 30.80761534725595 35.0 31.0 37.0 7.0 39.5 78-79 30.44587663914522 35.0 31.0 37.0 7.0 39.0 80-81 30.118445847498784 35.0 30.0 36.0 7.0 39.0 82-83 29.811355026711997 35.0 29.5 36.0 7.0 37.5 84-85 29.478290432248663 35.0 29.0 35.5 7.0 37.0 86-87 29.174579893152014 35.0 28.5 35.0 7.0 37.0 88-89 28.896940262263236 35.0 27.0 35.0 7.0 36.0 90-91 28.644895580378822 35.0 26.5 35.0 7.0 36.0 92-93 28.458892666342884 35.0 25.5 35.0 7.0 36.0 94-95 28.246770276833416 35.0 25.0 35.0 7.0 36.0 96-97 28.093443419135504 35.0 24.0 35.0 7.0 35.0 98-99 27.880077707625063 35.0 23.5 35.0 7.0 35.0 100-101 26.657212238950947 33.0 19.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.1794274658592805 1101 2 -0.8973271068870972 1101 3 -0.33311192588525174 1101 4 -0.1994029469366012 1101 5 0.3631730479368258 1101 6 0.27173191170039246 1101 7 -0.27347964379430323 1101 8 0.62425636383621 1101 9 0.4687889134330163 1101 10-11 0.063532242480143 1101 12-13 0.21216380646799138 1101 14-15 0.5212927494303585 1101 16-17 -0.3309190588528921 1101 18-19 -0.4054745319705688 1101 20-21 -0.004639707016487193 1101 22-23 -0.25323219702459454 1101 24-25 0.4342809100296492 1101 26-27 -0.14055334507649775 1101 28-29 -0.47180432410010553 1101 30-31 -0.25781662550134143 1101 32-33 -0.32352291198805716 1101 34-35 -0.11620826233326653 1101 36-37 -0.19365275217151634 1101 38-39 0.899705768062546 1101 40-41 0.8079028656723537 1101 42-43 0.05461225315418261 1101 44-45 0.7366803229876595 1101 46-47 -0.19376505177743297 1101 48-49 -0.011774376666288333 1101 50-51 0.12981338763282224 1101 52-53 0.1393272471610416 1101 54-55 0.8162973120935177 1101 56-57 0.4655936021356126 1101 58-59 0.39313779224996637 1101 60-61 1.0039991608198804 1101 62-63 -0.40820932864613724 1101 64-65 0.5460330178254011 1101 66-67 1.5412316006302902 1101 68-69 0.11055168118056002 1101 70-71 -0.16955813295857425 1101 72-73 0.39224200593388403 1101 74-75 -0.17365655732836416 1101 76-77 0.5454106573517201 1101 78-79 -0.6380092958767669 1101 80-81 -0.28867172460471835 1101 82-83 -0.2411110935642924 1101 84-85 -0.01615447411890969 1101 86-87 -0.6140867253034799 1101 88-89 0.031233026131491926 1101 90-91 0.837746871385729 1101 92-93 -0.4279224618498567 1101 94-95 -0.5511715099962728 1101 96-97 -0.017978120878893833 1101 98-99 0.5140029956503405 1101 100-101 0.8087260118201662 1102 1 -0.0687131801449965 1102 2 -0.3294699640299541 1102 3 -0.15811192588525103 1102 4 0.05773991020625857 1102 5 0.14174447650825073 1102 6 0.18958905455753694 1102 7 0.08009178477713164 1102 8 0.33854207812192527 1102 9 -0.3169253722812684 1102 10-11 0.3046036710515736 1102 12-13 0.3478780921822775 1102 14-15 0.9177213208589308 1102 16-17 1.2387237982899677 1102 18-19 0.515954039458002 1102 20-21 1.1667888644120836 1102 22-23 1.0431963744039763 1102 24-25 0.85570948145822 1102 26-27 0.8451609406377898 1102 28-29 1.058552818757036 1102 30-31 0.6278976602129447 1102 32-33 0.8354056594405179 1102 34-35 1.2409345948095876 1102 36-37 1.150990104971342 1102 38-39 1.8407771966339723 1102 40-41 0.8989742942437857 1102 42-43 1.399255110297041 1102 44-45 1.6777517515590858 1102 46-47 1.8223063767939962 1102 48-49 1.6417970519051437 1102 50-51 1.4101705304899639 1102 52-53 1.6732558185896096 1102 54-55 1.1323687406649512 1102 56-57 1.656665030707039 1102 58-59 1.793137792249965 1102 60-61 1.2575705893913138 1102 62-63 0.5096478142110072 1102 64-65 0.5174615892539762 1102 66-67 0.46623160063029445 1102 68-69 0.889123109751992 1102 70-71 0.47151329561285493 1102 72-73 1.2315277202195993 1102 74-75 1.06384344267164 1102 76-77 0.606124943066007 1102 78-79 0.33163356126609145 1102 80-81 0.9184711325381407 1102 82-83 0.9356746207214215 1102 84-85 0.5838455258810917 1102 86-87 0.8109132746965173 1102 88-89 1.2455187404172072 1102 90-91 0.775246871385729 1102 92-93 0.17922039529300093 1102 94-95 0.938114204289441 1102 96-97 1.53737902197825 1102 98-99 0.8854315670789141 1102 100-101 1.7730117261058815 1103 1 0.562697076265259 1103 2 0.655786446226454 1103 3 0.6258624330891074 1103 4 0.3910732435395943 1103 5 -0.36722988246610555 1103 6 0.45561469558317924 1103 7 0.6012456309309755 1103 8 0.9949523345321793 1103 9 0.8548694995135975 1103 10-11 0.697231876179778 1103 12-13 0.5619806562848382 1103 14-15 0.3747726029102125 1103 16-17 0.9826340547002275 1103 18-19 0.883902757406716 1103 20-21 1.0744811721043916 1103 22-23 0.4822989385065455 1103 24-25 0.8262223019710362 1103 26-27 0.6810583765352263 1103 28-29 0.12489897260319083 1103 30-31 0.7125130448283343 1103 32-33 0.6350851466200069 1103 34-35 1.1076012614762618 1103 36-37 0.08656702804826466 1103 38-39 0.002636170992943221 1103 40-41 -0.6994231416536536 1103 42-43 -1.4908089922670626 1103 44-45 -1.0809020945947552 1103 46-47 -0.5446808026931862 1103 48-49 -1.4421773070692225 1103 50-51 -1.8606628028433718 1103 52-53 -1.104628796795005 1103 54-55 -0.8115415157453043 1103 56-57 -0.851988815446802 1103 58-59 -0.9863493872372189 1103 60-61 -0.1699935131727912 1103 62-63 0.6474683270315182 1103 64-65 0.043102614894998226 1103 66-67 0.19764185704055137 1103 68-69 -0.9916461210172471 1103 70-71 -0.8794482428486852 1103 72-73 -1.4165492028573254 1103 74-75 -1.1377591214309248 1103 76-77 -1.1278494159083507 1103 78-79 -1.0859946438621115 1103 80-81 -1.1212724572054533 1103 82-83 -0.6499023023555033 1103 84-85 -0.7110262689907039 1103 86-87 -0.716650827867582 1103 88-89 0.055775150673614604 1103 90-91 -0.3955864619476053 1103 92-93 -0.8194975534249487 1103 94-95 -0.3676550264797882 1103 96-97 -0.7058902087909829 1103 98-99 -0.0991838175364741 1103 100-101 -0.06416776107360889 1104 1 0.1926504562186402 1104 2 0.5182573086973186 1104 3 0.9259789832056562 1104 4 0.2622853647517118 1104 5 0.15083538559915866 1104 6 0.22367996364844345 1104 7 0.18690996659530867 1104 8 -0.10236701278716964 1104 9 0.43762008226418203 1104 10-11 0.6284673074152138 1104 12-13 0.8842417285459163 1104 14-15 1.1268122299498344 1104 16-17 0.8398601619263246 1104 18-19 0.563681312185274 1104 20-21 0.7440615916848117 1104 22-23 0.8613781925857893 1104 24-25 0.9488912996400316 1104 26-27 0.9156154860923351 1104 28-29 0.755143727847944 1104 30-31 0.736988569303854 1104 32-33 0.9319965685314244 1104 34-35 0.9750255039004969 1104 36-37 0.9134901049713449 1104 38-39 0.7214590148157853 1104 40-41 0.9864742942437843 1104 42-43 1.702664201206133 1104 44-45 1.9357062970136312 1104 46-47 2.055260922248543 1104 48-49 2.6327061428142358 1104 50-51 2.2567614395808704 1104 52-53 2.0789376367714283 1104 54-55 2.740323286119498 1104 56-57 2.0509832125252174 1104 58-59 2.0567741558863233 1104 60-61 2.3416614984822175 1104 62-63 2.4755569051201007 1104 64-65 2.2333706801630697 1104 66-67 1.5889588733575692 1104 68-69 2.398214018842893 1104 70-71 1.5681042047037614 1104 72-73 1.5406186293105044 1104 74-75 1.653616169944364 1104 76-77 1.9356703976114602 1104 78-79 2.2100426521751864 1104 80-81 1.2752893143563178 1104 82-83 0.8868109843577834 1104 84-85 1.7202091622447284 1104 86-87 1.1927314565147 1104 88-89 1.5318823767808425 1104 90-91 1.7672923259311837 1104 92-93 2.015584031656637 1104 94-95 2.692659658834895 1104 96-97 2.479424476523704 1104 98-99 1.8422497488970926 1104 100-101 2.2866480897422434 1105 1 -1.4937131801449972 1105 2 -1.5294699640299534 1105 3 -1.6831119258852496 1105 4 -1.2922600897937428 1105 5 -1.1582555234917464 1105 6 -1.385410945442466 1105 7 -1.319908215222874 1105 8 -0.9864579218780776 1105 9 -1.341925372281274 1105 10-11 -1.0453963289484207 1105 12-13 -1.239621907817721 1105 14-15 -0.8322786791410692 1105 16-17 -1.0612762017100366 1105 18-19 -0.9715459605420023 1105 20-21 -1.2082111355879164 1105 22-23 -1.556803625596018 1105 24-25 -1.3567905185417786 1105 26-27 -1.417339059362213 1105 28-29 -1.3539471812429653 1105 30-31 -1.2596023397870582 1105 32-33 -1.5270943405594792 1105 34-35 -0.9340654051904096 1105 36-37 -1.7990098950286608 1105 38-39 -1.5592228033660334 1105 40-41 -1.6135257057562171 1105 42-43 -1.4632448897029633 1105 44-45 -1.6847482484409184 1105 46-47 -1.3026936232060038 1105 48-49 -1.1207029480948592 1105 50-51 0.04767053048996672 1105 52-53 -0.23924418141039183 1105 54-55 -0.25513125933505165 1105 56-57 0.2566650307070404 1105 58-59 -0.6193622077500365 1105 60-61 -1.129929410608689 1105 62-63 0.009647814211007244 1105 64-65 -0.04503841074602377 1105 66-67 -0.24626839936970413 1105 68-69 -0.8858768902480136 1105 70-71 -0.053486704387143646 1105 72-73 -0.09347227978040351 1105 74-75 -0.5361565573283613 1105 76-77 0.03112494306600766 1105 78-79 0.20663356126609145 1105 80-81 -0.544028867461865 1105 82-83 -0.41432537927857993 1105 84-85 0.5088455258810924 1105 86-87 0.7359132746965216 1105 88-89 0.17051874041720438 1105 90-91 1.1627468713857319 1105 92-93 0.3917203952929995 1105 94-95 0.23811420428944174 1105 96-97 0.14987902197825065 1105 98-99 0.3354315670789134 1105 100-101 0.1980117261058787 1106 1 0.20212015318833565 1106 2 0.5371967026367095 1106 3 0.2877214074480854 1106 4 0.12023991020625857 1106 5 0.42924447650825215 1106 6 0.21042238789087264 1106 7 0.1925917847771288 1106 8 0.05104207812192385 1106 9 0.3164079610520645 1106 10-11 0.1379370043849022 1106 12-13 0.11246142551560467 1106 14-15 0.16563798752559222 1106 16-17 0.5178904649566363 1106 18-19 0.2805373727913292 1106 20-21 0.21470553107874935 1106 22-23 0.09527970773730488 1106 24-25 0.2036261481248829 1106 26-27 0.40141094063778837 1106 28-29 0.15855281875703753 1106 30-31 -0.4116856731203882 1106 32-33 -0.5916776738928178 1106 34-35 0.2326012614762618 1106 36-37 0.25724010497134486 1106 38-39 0.3303605299673009 1106 40-41 -0.0343590390895514 1106 42-43 -0.38824488970296045 1106 44-45 -0.8389149151075799 1106 46-47 -0.3860269565393395 1106 48-49 -0.36445294809485773 1106 50-51 -0.9544128028433718 1106 52-53 -0.18716084807705613 1106 54-55 0.3823687406649512 1106 56-57 0.23374836404037325 1106 58-59 0.7868877922499635 1106 60-61 -0.05701274394202471 1106 62-63 -0.26118551912232846 1106 64-65 -0.013788410746023771 1106 66-67 0.2953982672969673 1106 68-69 0.541206443085315 1106 70-71 0.8985966289461906 1106 72-73 -0.1393056131137378 1106 74-75 0.39509344267163726 1106 76-77 0.512374943066007 1106 78-79 0.9941335612660929 1106 80-81 0.8434711325381379 1106 82-83 1.064841287388088 1106 84-85 0.8963455258810917 1106 86-87 -0.6036700586368156 1106 88-89 -0.2086479262494585 1106 90-91 -0.24975312861426957 1106 92-93 -0.5895296047069998 1106 94-95 -0.27230246237722255 1106 96-97 0.49362902197825065 1106 98-99 0.6708482337455806 1106 100-101 0.43342839277254797 1107 1 0.18383784026316619 1107 2 0.7965504441333131 1107 3 0.5117860332984243 1107 4 0.36386235918585186 1107 5 0.5019485581409029 1107 6 0.36560946272080486 1107 7 0.4489693357975355 1107 8 0.18752166995865593 1107 9 0.2279725869024034 1107 10-11 0.696185303704631 1107 12-13 0.3550209493251302 1107 14-15 0.54170091269566 1107 16-17 0.5323462472695581 1107 18-19 0.42105608027432595 1107 20-21 0.3280133542080037 1107 22-23 0.428400456036627 1107 24-25 0.1995870324786253 1107 26-27 0.3778140018622764 1107 28-29 0.33839975753254947 1107 30-31 0.18504051735580873 1107 32-33 -0.6684208711717261 1107 34-35 0.11440398256469564 1107 36-37 -0.11202009911028199 1107 38-39 0.018073115001321582 1107 40-41 0.49157633506010967 1107 42-43 -1.092326522356018 1107 44-45 -0.7041360035429562 1107 46-47 0.24705127475318278 1107 48-49 0.18210317435411838 1107 50-51 0.35022155089812657 1107 52-53 0.3781027573651201 1107 54-55 0.9244605773996497 1107 56-57 0.37171605111520023 1107 58-59 0.38701534327037024 1107 60-61 0.22848895673825353 1107 62-63 0.3387294468640647 1107 64-65 0.6195024055805121 1107 66-67 0.48204792716091305 1107 68-69 0.2901435179152543 1107 70-71 0.47533982622509896 1107 72-73 0.7004052712400046 1107 74-75 0.6610373202226576 1107 76-77 0.37398208592315285 1107 78-79 0.5987253980007843 1107 80-81 0.4480629692728293 1107 82-83 0.18133788602754208 1107 84-85 -0.05901161697605417 1107 86-87 0.3246887849006015 1107 88-89 0.4516411893967991 1107 90-91 0.11121625914083566 1107 92-93 0.6292203952930002 1107 94-95 0.5059713471465841 1107 96-97 0.5758994301415186 1107 98-99 1.0099213629972787 1107 100-101 0.17811376692220549 1108 1 0.8723245557040613 1108 2 1.1323224888002308 1108 3 0.47443524392607017 1108 4 0.9634002875647525 1108 5 0.9728765519799509 1108 6 0.8900607526707418 1108 7 0.8494314074186349 1108 8 1.2291081158577697 1108 9 1.0585463258319336 1108 10-11 1.1807829163345929 1108 12-13 0.8469346959558663 1108 14-15 1.1398911321796845 1108 16-17 1.2167898360258107 1108 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0.5023694376228853 2211 82-83 0.6545305529248111 2211 84-85 1.2109641699488911 2211 86-87 0.7503200543575375 2211 88-89 0.7302645031290709 2211 90-91 0.5648655154535298 2211 92-93 0.5360000563099483 2211 94-95 0.6004023398826597 2211 96-97 0.092675632147742 2211 98-99 0.6159400416551826 2211 100-101 0.3467405396652019 2212 1 0.5821488888205195 2212 2 0.8834610704528068 2212 3 0.7841294534250913 2212 4 0.9887743929648778 2212 5 0.9572617178875618 2212 6 0.7111407786954658 2212 7 0.686126267535748 2212 8 0.7213006988115822 2212 9 0.8796263518566576 2212 10-11 0.8800347055343352 2212 12-13 0.3685677473546889 2212 14-15 0.04099718292789589 2212 16-17 0.3727755224279008 2212 18-19 0.4159540394580006 2212 20-21 0.5426509333776011 2212 22-23 -0.11628638421671411 2212 24-25 0.48846810214786984 2212 26-27 0.27964369925847876 2212 28-29 0.4882941980673863 2212 30-31 0.38824248779915393 2212 32-33 0.8548022111646603 2212 34-35 -0.11078954312144162 2212 36-37 -0.27616506744244873 2212 38-39 0.18344961042707553 2212 40-41 0.02957774251964196 2212 42-43 -0.05203799315123092 2212 44-45 0.16180347569702036 2212 46-47 -0.028986726654274264 2212 48-49 0.41679705190514227 2212 50-51 0.7830153580761703 2212 52-53 0.26937650824478254 2212 54-55 -0.5206485007143655 2212 56-57 -0.22997290032743933 2212 58-59 -0.34996565602589413 2212 60-61 -0.05880872095351464 2212 62-63 -0.546386668547612 2212 64-65 -1.1549522038494722 2212 66-67 -1.791527020059359 2212 68-69 -2.1315665454204265 2212 70-71 -2.047883256111284 2212 72-73 -0.9245067625390213 2212 74-75 -0.9357255228456012 2212 76-77 -0.06197850520985426 2212 78-79 -1.3808664387339071 2212 80-81 -1.100494384703243 2212 82-83 -1.2440667585889251 2212 84-85 -0.8902924051533923 2212 86-87 -0.32960396668278946 2212 88-89 -1.251032983720723 2212 90-91 -1.3381151975797856 2212 92-93 -1.1552623633276902 2212 94-95 -0.6368857957105583 2212 96-97 -0.4578795987114077 2212 98-99 0.4837074291478807 2212 100-101 0.2505979330024317 2213 1 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0.9841121207214201 2214 84-85 1.0682205258810917 2214 86-87 0.8468507746965201 2214 88-89 0.5205187404172058 2214 90-91 0.9221218713857304 2214 92-93 0.6135953952930002 2214 94-95 0.7381142042894417 2214 96-97 0.8608165219782506 2214 98-99 1.0588690670789127 2214 100-101 1.0401992261058801 2215 1 0.6213553130056866 2215 2 0.6427903099426509 2215 3 0.14360040288187292 2215 4 0.7974659376035191 2215 5 0.7588677641794845 2215 6 0.5132191915438398 2215 7 0.3304342505305513 2215 8 -0.06967709996026628 2215 9 0.5721157236091372 2215 10-11 0.12224065735293976 2215 12-13 0.04582329766172677 2215 14-15 0.6543651564753645 2215 16-17 0.6371827023995564 2215 18-19 0.1317074641155358 2215 20-21 0.3010354397545498 2215 22-23 0.09627856618479314 2215 24-25 -0.13812613498013349 2215 26-27 0.42324313241860523 2215 28-29 -0.12483759220186386 2215 30-31 -0.3227872712938975 2215 32-33 -0.4137381761759187 2215 34-35 -0.04536677505342368 2215 36-37 0.7251339405877815 2215 38-39 0.418688155538085 2215 40-41 0.49674826684652373 2215 42-43 0.20079620618744798 2215 44-45 0.4707312036138731 2215 46-47 0.4772721302186582 2215 48-49 0.300358695740762 2215 50-51 0.06856094144886526 2215 52-53 0.11349554461700251 2215 54-55 0.07500572696631735 2215 56-57 0.4051239348166291 2215 58-59 0.4328638196472241 2215 60-61 0.48736510993925464 2215 62-63 0.7784834306493593 2215 64-65 0.22979035637726497 2215 66-67 1.064519271863169 2215 68-69 0.465150507012261 2215 70-71 0.6864105558868268 2215 72-73 0.4448838846031613 2215 74-75 0.5359324837675246 2215 76-77 0.9982482307372393 2215 78-79 0.4136541092112971 2215 80-81 0.22189579007238436 2215 82-83 -0.11723633818268908 2215 84-85 0.09754415601807764 2215 86-87 -0.3538127527007404 2215 88-89 -0.4024264650622449 2215 90-91 -0.44581477244988577 2215 92-93 0.3004532720053277 2215 94-95 -0.08894059023110756 2215 96-97 -0.7696415259669536 2215 98-99 -0.9542944603183479 2215 100-101 -0.3235636163598734 2216 1 -0.23916772559954325 2216 2 -0.4687556783156701 2216 3 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0.6391084840069503 2316 90-91 0.41851610215496393 2316 92-93 0.4240921901647958 2316 94-95 0.27337061454585054 2316 96-97 0.02487902197825065 2316 98-99 0.4841495157968616 2316 100-101 0.7627553158494713 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 155.0 8 337.0 9 261.0 10 184.0 11 219.0 12 207.0 13 218.0 14 194.0 15 184.0 16 290.0 17 447.0 18 613.0 19 761.0 20 980.0 21 1005.0 22 934.0 23 755.0 24 616.0 25 549.0 26 567.0 27 594.0 28 568.0 29 582.0 30 642.0 31 800.0 32 964.0 33 1224.0 34 1700.0 35 4289.0 36 4376.0 37 7798.0 38 13432.0 39 5007.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.61971830985916 22.824672170956774 15.578436134045653 16.977173385138418 2 16.90529383195726 25.81835842642059 38.572122389509474 18.704225352112676 3 17.21223895094706 32.68576979116076 33.35794074793589 16.744050509956292 4 10.741136474016514 23.933948518698397 38.98008742107819 26.344827586206897 5 9.82030111704711 40.30305973773676 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33.38028169014085 18.027197668771247 52-53 16.47887323943662 32.47207382224381 33.36279747450219 17.68625546381739 54-55 16.387566779990287 32.57115104419621 33.39290917921321 17.648372996600294 56-57 15.972802331228753 32.699368625546384 33.24526469159787 18.082564351627003 58-59 16.259349198640116 32.08644973288004 33.19572608062166 18.458474987858185 60-61 16.201068479844587 32.1534725594949 33.011170471102474 18.63428848955804 62-63 16.4108790675085 31.633802816901408 33.38319572608062 18.57212238950947 64-65 16.027197668771247 31.712481787275376 33.377367654201066 18.882952889752307 66-67 16.099077221952403 31.851384167071394 32.60903351141331 19.440505099562895 68-69 16.225352112676056 31.26469159786304 32.886838271005345 19.62311801845556 70-71 16.338028169014084 31.425934919864012 32.19524040796503 20.040796503156873 72-73 16.400194269062652 30.824672170956774 32.095191840699364 20.679941719281207 74-75 16.50509956289461 30.688683827100533 31.86012627489072 20.946090335114135 76-77 16.491500728508985 31.08693540553667 32.15055852355512 20.271005342399224 78-79 16.86352598348713 30.352598348712966 32.00097134531326 20.782904322486644 80-81 16.49635745507528 30.425449247207382 31.63477416221467 21.44341913550267 82-83 16.86546867411365 30.141816415735796 32.07867897037397 20.91403593977659 84-85 16.165128703254005 30.064108790675085 31.942690626517727 21.828071879553182 86-87 16.608062166100048 29.93200582807188 31.911607576493445 21.548324429334627 88-89 17.290917921321032 29.183098591549296 31.776590577950458 21.749392909179214 90-91 17.243322000971347 29.772705196697423 31.391937833899952 21.59203496843128 92-93 17.27343370568237 29.38222438076736 31.763963088878093 21.580378824672174 94-95 17.635745507527925 28.678970373967942 31.743564837299658 21.94171928120447 96-97 18.069936862554638 28.27197668771248 31.711510441962115 21.946576007770762 98-99 17.876639145216124 28.35065565808645 31.294803302574064 22.477901894123363 100-101 18.290432248664402 27.997085964060226 31.738708110733366 21.97377367654201 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1422.0 1 827.5 2 273.0 3 318.0 4 225.0 5 147.0 6 264.0 7 523.0 8 677.5 9 647.0 10 469.5 11 276.0 12 229.5 13 220.0 14 214.0 15 200.0 16 181.0 17 179.5 18 199.0 19 199.0 20 216.0 21 251.0 22 260.0 23 303.5 24 360.5 25 371.5 26 391.0 27 457.5 28 530.0 29 611.5 30 718.5 31 881.5 32 1078.5 33 1283.0 34 1555.5 35 1714.5 36 1897.5 37 2170.0 38 2409.0 39 2539.0 40 2399.5 41 2212.5 42 2110.5 43 1983.0 44 1743.0 45 1579.0 46 1449.5 47 1265.5 48 1218.0 49 1205.5 50 1151.5 51 1050.5 52 881.0 53 680.0 54 501.0 55 379.0 56 318.0 57 306.5 58 273.5 59 263.0 60 247.0 61 186.0 62 166.0 63 165.0 64 139.0 65 110.5 66 88.5 67 73.5 68 55.5 69 38.5 70 39.5 71 39.0 72 31.0 73 22.5 74 18.0 75 18.0 76 17.0 77 14.5 78 10.5 79 8.5 80 7.0 81 7.0 82 4.5 83 4.5 84 4.0 85 2.0 86 1.5 87 2.0 88 1.5 89 0.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.713453132588635E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 51475.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.191840699368626 #Duplication Level Percentage of deduplicated Percentage of total 1 76.43732685666578 39.129674599320055 2 11.411331638267997 11.683341427877611 3 4.276877537854351 6.5682370082564345 4 2.2503889795453684 4.608062166100049 5 1.4193009752950552 3.6328314715881493 6 0.906986452127054 2.7858183584264204 7 0.7172403324352017 2.570179698882953 8 0.4743652992296308 1.942690626517727 9 0.32636332586998595 1.5036425449247208 >10 1.7115099996205079 14.191355026711996 >50 0.018974611969185227 0.5905779504613891 >100 0.03415430154453341 3.8931520155415247 >500 0.011384767181511138 4.507042253521127 >1k 0.0037949223938370464 2.39339485186984 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1232 2.39339485186984 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 821 1.594949004371054 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 768 1.4919864011656145 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 731 1.4201068479844585 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 478 0.9286061194754734 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 349 0.6779990286546868 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 305 0.5925206410879067 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 249 0.48372996600291407 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 157 0.3050024283632831 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 0.27003399708596404 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 111 0.2156386595434677 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 110 0.21369596891694995 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 106 0.20592520641087908 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64 0.12433220009713453 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 62 0.12044681884409907 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 61 0.11850412821758136 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60 0.11656143759106363 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 57 0.11073336571151043 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0029140359397765905 0.0 18-19 0.0 0.0 0.0 0.003885381253035454 0.0 20-21 0.0 0.0 0.0 0.0048567265662943174 0.0 22-23 0.0 0.0 0.0 0.015541525012141816 0.0 24-25 0.0 0.0 0.0 0.029140359397765908 0.0 26-27 0.0 0.0 0.0 0.07382224380767363 0.0 28-29 0.0 0.0 0.0 0.1864983001457018 0.0 30-31 0.0 0.0 0.0 0.3428848955803788 0.0 32-33 0.0 0.0 0.0 0.4847013113161729 0.0 34-35 0.0 0.0 0.0 0.7013113161728994 0.0 36-37 0.0 0.0 0.0 1.0004856726566294 0.0 38-39 0.0 0.0 0.0 1.3210296260320544 0.0 40-41 0.0 0.0 0.0 1.8173870811073336 0.0 42-43 0.0 0.0 0.0 2.118504128217581 0.0 44-45 0.0 0.0 0.0 2.376881981544439 0.0 46-47 0.0 0.0 0.0 2.5798931520155417 0.0 48-49 0.0 0.0 0.0 2.8237008256435163 0.0 50-51 0.0 0.0 0.0 3.0539096648858672 0.0 52-53 0.0 0.0 0.0 3.2268091306459445 0.0 54-55 0.0 0.0 0.0 3.501699854298203 0.0 56-57 0.0 0.0 0.0 3.852355512384653 0.0 58-59 0.0 0.0 0.0 4.177756192326372 0.0 60-61 0.0 0.0 0.0 4.413793103448276 0.0 62-63 0.0 0.0 0.0 4.647887323943662 0.0 64-65 0.0 0.0 0.0 4.886838271005342 0.0 66-67 0.0 0.0 0.0 5.1500728508984945 0.0 68-69 0.0 0.0 0.0 5.468674113647402 0.0 70-71 0.0 0.0 0.0 5.8358426420592515 0.0 72-73 0.0 0.0 0.0 6.177756192326372 0.0 74-75 0.0 0.0 0.0 6.549781447304516 0.0 76-77 0.0 0.0 0.0 6.916949975716367 0.0 78-79 0.0 0.0 0.0 7.318115590092278 0.0 80-81 0.0 0.0 0.0 7.725109276347741 0.0 82-83 0.0 0.0 0.0 8.150558523555123 0.0 84-85 0.0 0.0 0.0 8.557552209810588 0.0 86-87 0.0 0.0 0.0 8.985915492957748 0.0 88-89 0.0 0.0 0.0 9.433705682370082 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTCCT 25 0.0049941284 57.0 9 GGTATCA 595 0.0 56.680676 1 TACCCAT 45 9.5394626E-4 42.22222 2 ACCCATG 45 9.5394626E-4 42.22222 3 GTATCAA 840 0.0 40.14881 2 CGCAGAA 135 7.2759576E-12 38.7037 9 GCAGAGA 60 3.7234713E-9 35.625 10-11 CAACGCA 990 0.0 34.06566 6 TATCAAC 1005 0.0 33.557217 3 TCAACGC 1005 0.0 33.557217 5 ATCAACG 1010 0.0 33.39109 4 AACGCAG 1005 0.0 33.08458 7 GTACCCA 60 0.0039162757 31.666668 1 AGCCTTT 30 0.003934401 31.666668 36-37 ACGCAGA 1065 0.0 30.774649 8 TAGATTT 65 0.005793011 29.230766 4 CGCAGAG 950 0.0 29.0 9 ATGGGGA 35 0.00835673 27.142859 18-19 GAGTACT 525 0.0 26.690474 12-13 GTACTTT 555 0.0 25.247747 14-15 >>END_MODULE