FastQCFastQC Report
Fri 10 Feb 2017
SRR2031778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51475
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT13222.568237008256435No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11212.177756192326372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9651.8746964545896065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9381.822243807673628No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8711.6920835356969404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6081.1811559009227781No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.7829043224866441No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.5497814473045167No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT2760.5361826129188927RNA PCR Primer, Index 33 (95% over 22bp)
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2420.4701311316172899No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT1810.3516270033997086RNA PCR Primer, Index 33 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.3166585721223895No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.2525497814473045No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.23895094706168044No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG960.18649830014570182RNA PCR Primer, Index 33 (96% over 29bp)
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.16318601262748908No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT760.14764448761534726No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.14375910636231182No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT740.14375910636231182RNA PCR Primer, Index 33 (96% over 27bp)
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.13987372510927634No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG590.1146187469645459No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT540.10490529383195726No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT540.10490529383195726No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG530.10296260320543954No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.10296260320543954No Hit
TTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCC520.1010199125789218No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTG156.582006E-494.999049
TAGAGGA200.002062883571.249274
TTAATCT250.004994330556.9994164
ATCTAAC250.004994330556.9994168
TCTAACA250.004994330556.9994169
CGCAGAA602.293018E-855.4161079
GGTATCA5750.046.3053971
AGTGGCT500.001601588137.999618
CTACTGA600.003897838331.697151
ACTATAC902.3689563E-531.6663423
GTATCAA9000.030.112291
CATGGGG1302.9429975E-729.230474
CTTGAAA2950.028.98275456-57
GCTTGAA3100.027.58036256-57
TGCTTGA3200.027.46065554-55
CAACGCA9900.027.3482066
TCAACGC9950.027.2107775
GGTTTCC350.00835714927.14257870-71
TATAGTG700.00831899227.1425785
AACGCAG9900.026.8684127
Produced by FastQC (version 0.11.5)