##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031778_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51475 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73831957260806 34.0 31.0 34.0 31.0 34.0 2 32.84281690140845 34.0 33.0 34.0 31.0 34.0 3 32.81336571151044 34.0 33.0 34.0 31.0 34.0 4 36.25971830985915 37.0 37.0 37.0 35.0 37.0 5 36.323069451189895 37.0 37.0 37.0 35.0 37.0 6 36.43545410393395 37.0 37.0 37.0 35.0 37.0 7 36.38148615832929 37.0 37.0 37.0 35.0 37.0 8 36.44854783875668 37.0 37.0 37.0 35.0 37.0 9 38.19757163671685 39.0 39.0 39.0 37.0 39.0 10-11 38.23271491015056 39.0 39.0 39.0 37.0 39.0 12-13 38.238241864982996 39.0 39.0 39.0 37.0 39.0 14-15 39.66073822243808 41.0 40.0 41.0 37.0 41.0 16-17 39.567382224380765 41.0 39.0 41.0 37.0 41.0 18-19 39.60103933948518 41.0 40.0 41.0 36.5 41.0 20-21 39.60897523069451 41.0 40.0 41.0 36.0 41.0 22-23 39.475007285089845 41.0 39.0 41.0 35.0 41.0 24-25 39.338863525983484 41.0 39.0 41.0 35.0 41.0 26-27 39.111627003399704 41.0 38.5 41.0 35.0 41.0 28-29 38.93965031568723 41.0 38.0 41.0 35.0 41.0 30-31 38.63102476930548 40.0 37.5 41.0 35.0 41.0 32-33 37.951860126274894 40.0 35.0 41.0 35.0 41.0 34-35 37.87911607576494 40.0 35.0 41.0 35.0 41.0 36-37 37.690296260320544 40.0 35.0 41.0 35.0 41.0 38-39 37.32530354541039 40.0 35.0 41.0 33.5 41.0 40-41 37.191199611461876 40.0 35.0 41.0 33.0 41.0 42-43 36.914657600777076 40.0 35.0 41.0 33.0 41.0 44-45 36.52508984944147 40.0 35.0 41.0 32.0 41.0 46-47 36.11311316172899 40.0 35.0 41.0 29.0 41.0 48-49 35.76127246236037 40.0 35.0 41.0 24.0 41.0 50-51 35.52166100048567 40.0 35.0 41.0 22.0 41.0 52-53 35.51491015055852 40.0 35.0 41.0 20.5 41.0 54-55 35.40712967459932 40.0 35.0 41.0 20.0 41.0 56-57 35.18844099077222 39.5 35.0 41.0 18.0 41.0 58-59 34.961622146673136 39.0 35.0 41.0 17.5 41.0 60-61 34.66206896551724 39.0 35.0 41.0 15.0 41.0 62-63 34.308343856240896 38.5 35.0 41.0 11.5 41.0 64-65 33.94667314230209 37.5 35.0 41.0 10.0 41.0 66-67 33.521699854298205 37.0 35.0 40.5 10.0 41.0 68-69 32.92766391452162 36.5 34.0 40.0 9.0 41.0 70-71 32.346886838271004 36.0 33.5 39.0 8.0 41.0 72-73 31.820582807187954 35.5 33.0 39.0 8.0 41.0 74-75 31.445186983972803 35.0 33.0 38.5 7.0 40.5 76-77 30.15853326857698 34.5 30.0 36.5 7.0 39.0 78-79 30.69947547353084 35.0 31.5 37.0 7.0 39.0 80-81 30.527489072365228 35.0 32.0 36.5 7.0 39.0 82-83 30.233977659057796 35.0 31.0 36.0 7.0 37.5 84-85 29.96866440019427 35.0 31.0 36.0 7.0 37.0 86-87 29.757872753763962 35.0 31.0 35.0 7.0 37.0 88-89 29.54288489558038 35.0 30.5 35.0 7.0 36.0 90-91 29.327867897037397 35.0 30.0 35.0 7.0 36.0 92-93 29.15013113161729 35.0 29.5 35.0 7.0 36.0 94-95 29.010208839242352 35.0 29.0 35.0 7.0 36.0 96-97 28.91541525012142 35.0 29.0 35.0 7.0 35.0 98-99 28.79256920835357 35.0 29.0 35.0 7.0 35.0 100-101 27.915356969402623 34.0 26.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41828836486163823 1101 2 0.33458160538300774 1101 3 0.26015606352152076 1101 4 -0.1648294862216133 1101 5 0.030563475961166375 1101 6 -0.5853679601976296 1101 7 0.07375229114332171 1101 8 -0.493417849774211 1101 9 0.06586059541114508 1101 10-11 0.1055891923268959 1101 12-13 0.22940602341078886 1101 14-15 0.27020116624256474 1101 16-17 -0.17122290621436775 1101 18-19 0.07059217750112623 1101 20-21 0.5185419609184265 1101 22-23 0.638009500293002 1101 24-25 0.286203407438272 1101 26-27 0.031353767135755106 1101 28-29 0.16481472923703677 1101 30-31 0.3445574523757031 1101 32-33 0.15793752183042642 1101 34-35 0.048381619878725246 1101 36-37 0.33873507818015725 1101 38-39 0.6878388007907432 1101 40-41 0.8702647065746092 1101 42-43 0.7041887973592154 1101 44-45 0.8624711426626988 1101 46-47 -0.15278001691942933 1101 48-49 -0.2299830150508555 1101 50-51 1.2708483061638063 1101 52-53 1.0271256566456088 1101 54-55 1.310987405655574 1101 56-57 0.7786627624896312 1101 58-59 0.427826026004432 1101 60-61 1.6587664820233314 1101 62-63 1.999203066021309 1101 64-65 2.2826773855257656 1101 66-67 2.3027846074867853 1101 68-69 2.600320742541541 1101 70-71 1.3396559650482587 1101 72-73 1.0540005182258056 1101 74-75 1.6740527397528844 1101 76-77 1.4844919163043464 1101 78-79 0.5015080757525574 1101 80-81 0.8679237721461028 1101 82-83 0.45850091411120886 1101 84-85 0.6785717856512576 1101 86-87 1.2595109291439037 1101 88-89 1.2912896617334866 1101 90-91 0.8936072349293376 1101 92-93 1.1090024718870453 1101 94-95 0.6064232608610034 1101 96-97 0.5572380309562313 1101 98-99 1.3285600826064332 1101 100-101 0.967885978698785 1102 1 4.312220044937476E-4 1102 2 0.18458160538300206 1102 3 0.42801320637866525 1102 4 0.1494562280640963 1102 5 0.16270633310402616 1102 6 0.3074891826595163 1102 7 0.5166094340004648 1102 8 0.21372500736864453 1102 9 -0.11271083316028552 1102 10-11 0.3377320494697571 1102 12-13 0.31869173769650416 1102 14-15 0.5594868805282758 1102 16-17 0.8966342366427682 1102 18-19 0.7205921775011248 1102 20-21 0.7381848180612778 1102 22-23 0.7397952145787201 1102 24-25 0.7272748360096983 1102 26-27 0.7849251957071814 1102 28-29 0.7076718720941813 1102 30-31 0.6999145952328476 1102 32-33 0.6579375218304264 1102 34-35 0.4823101913072989 1102 36-37 0.1565922210373003 1102 38-39 0.49498165793360016 1102 40-41 -0.236878150568252 1102 42-43 0.5524030830735001 1102 44-45 0.6946139998055614 1102 46-47 1.0168628402234319 1102 48-49 0.3771598420920057 1102 50-51 0.03513402044952585 1102 52-53 0.6592685137884686 1102 54-55 0.7663445485127127 1102 56-57 0.40009133391820484 1102 58-59 0.1813974545758512 1102 60-61 0.18019505345190368 1102 62-63 0.46706020887845057 1102 64-65 0.7576773855257599 1102 66-67 -0.02757253537036064 1102 68-69 0.402106456827255 1102 70-71 1.082513107905399 1102 72-73 0.971857661082943 1102 74-75 0.8311955968957427 1102 76-77 0.20949191630434782 1102 78-79 -0.9556347813902981 1102 80-81 -0.8552905135681819 1102 82-83 -0.23614194303165092 1102 84-85 -0.16249964292016728 1102 86-87 -0.23156049942752333 1102 88-89 -0.6729960525522287 1102 90-91 -1.4921070507849485 1102 92-93 -0.9070689566843839 1102 94-95 -0.3150053105675674 1102 96-97 -0.540976254758057 1102 98-99 -0.6892970602507091 1102 100-101 -1.153542592729785 1103 1 -0.0066200600467922754 1103 2 0.3653508361522384 1103 3 -0.6174996141341538 1103 4 -0.3787489001410336 1103 5 -0.004601359203668665 1103 6 0.044668669839005304 1103 7 -0.3064674890764536 1103 8 0.15346859711223715 1103 9 0.042417371967921724 1103 10-11 0.40375769049538945 1103 12-13 0.10266609667085902 1103 14-15 -0.4744874784460791 1103 16-17 -0.38734012233159376 1103 18-19 -0.014023207114263414 1103 20-21 0.23465917703563832 1103 22-23 0.19588495816846319 1103 24-25 0.15323637447124128 1103 26-27 0.2830021187841041 1103 28-29 0.276902641324952 1103 30-31 0.2745940824123352 1103 32-33 -0.15744709355418962 1103 34-35 0.05827172976884043 1103 36-37 -0.3953308558857813 1103 38-39 -0.18578757283562908 1103 40-41 -0.15867302236312497 1103 42-43 -0.12548153231111314 1103 44-45 0.5093575895491469 1103 46-47 0.8171833530439372 1103 48-49 -0.7446350297028701 1103 50-51 -1.166148030832531 1103 52-53 -2.3721417426217926 1103 54-55 -2.1487195540513895 1103 56-57 -2.249908666081801 1103 58-59 -1.6333461351677343 1103 60-61 -1.628779305522464 1103 62-63 -1.8297346629164224 1103 64-65 -1.7839892811409044 1103 66-67 -1.74263663793446 1103 68-69 -1.3674448252240268 1103 70-71 -0.8379997126074201 1103 72-73 -0.862116697891409 1103 74-75 -0.30983004412989956 1103 76-77 -0.8796106477982129 1103 78-79 -1.1787117044672222 1103 80-81 -0.5921494879271592 1103 82-83 -0.7345393789290888 1103 84-85 -0.51153810445863 1103 86-87 -0.7434194737864992 1103 88-89 0.24879881924264424 1103 90-91 -0.22544038411828282 1103 92-93 0.02402078690536058 1103 94-95 -0.24705659261884705 1103 96-97 -0.23616856245036288 1103 98-99 -0.17327141922506684 1103 100-101 -0.747132336319531 1104 1 0.09361304018631245 1104 2 -0.04723657643517498 1104 3 0.33937684274229696 1104 4 0.06763804624591785 1104 5 -0.10774821235052201 1104 6 -0.010692635522303817 1104 7 0.2984276158186461 1104 8 0.31372500736864595 1104 9 0.49638007593062383 1104 10-11 0.44455023128794124 1104 12-13 0.4482371922419546 1104 14-15 0.4753959714373721 1104 16-17 0.5170887820973178 1104 18-19 0.3705921775011234 1104 20-21 0.5006848180612806 1104 22-23 0.19093157821507845 1104 24-25 -0.1920433458084787 1104 26-27 0.25765246843445766 1104 28-29 -0.3673281279058216 1104 30-31 -0.7921308593126071 1104 32-33 0.17384661273951707 1104 34-35 0.3607192822163938 1104 36-37 0.056592221037298884 1104 38-39 -0.26183652388458967 1104 40-41 0.031303667613563846 1104 42-43 0.20581217398259355 1104 44-45 0.04461399980555569 1104 46-47 0.20209011295069956 1104 48-49 0.7862507511829122 1104 50-51 2.1578612931767935 1104 52-53 1.6797230592430097 1104 54-55 1.7606627303308997 1104 56-57 2.000091333918199 1104 58-59 2.092761090939497 1104 60-61 2.0142859625428073 1104 62-63 2.32615111796936 1104 64-65 2.212222840071213 1104 66-67 1.973563828266009 1104 68-69 2.3464246386454377 1104 70-71 1.6529676533599442 1104 72-73 0.34913038835568244 1104 74-75 1.0811955968957427 1104 76-77 1.6163100981225291 1104 78-79 1.3239106731551544 1104 80-81 1.427664031886362 1104 82-83 1.8718126024228958 1104 84-85 1.64318217526165 1104 86-87 1.651394046027022 1104 88-89 1.4338221292659554 1104 90-91 1.5624384037605061 1104 92-93 1.394067406951983 1104 94-95 1.6940855985233405 1104 96-97 1.1283419270601236 1104 98-99 1.0493393033856542 1104 100-101 1.6328210436338466 1105 1 0.35043122200449517 1105 2 0.2595816053830049 1105 3 0.253013206378661 1105 4 0.12445622806409773 1105 5 0.3377063331040233 1105 6 -0.11751081734048086 1105 7 0.3916094340004648 1105 8 0.3887250073686488 1105 9 0.31228916683971875 1105 10-11 -0.2872679505302429 1105 12-13 -0.09380826230349726 1105 14-15 0.29698688052828004 1105 16-17 -0.06586576335723038 1105 18-19 0.20809217750112197 1105 20-21 0.17568481806127778 1105 22-23 0.5522952145787201 1105 24-25 0.26477483600969975 1105 26-27 0.8974251957071857 1105 28-29 0.5451718720941798 1105 30-31 0.46241459523284334 1105 32-33 1.1454375218304236 1105 34-35 0.6823101913072946 1105 36-37 0.7065922210373046 1105 38-39 0.6449816579335987 1105 40-41 0.7381218494317565 1105 42-43 0.7524030830735029 1105 44-45 0.7196139998055529 1105 46-47 1.191862840223429 1105 48-49 1.2771598420920043 1105 50-51 1.3226340204495202 1105 52-53 1.384268513788463 1105 54-55 1.6538445485127156 1105 56-57 1.5875913339181977 1105 58-59 1.0438974545758626 1105 60-61 0.4926950534519037 1105 62-63 0.7920602088784534 1105 64-65 0.8576773855257613 1105 66-67 1.3349274646296436 1105 68-69 0.26460645682725925 1105 70-71 0.032513107905394634 1105 72-73 0.3343576610829473 1105 74-75 0.5811955968957427 1105 76-77 1.259491916304352 1105 78-79 0.019365218609699753 1105 80-81 -0.40529051356818613 1105 82-83 0.2388580569683505 1105 84-85 -0.21249964292017154 1105 86-87 -0.43156049942752617 1105 88-89 -0.1479960525522266 1105 90-91 -0.5671070507849443 1105 92-93 -0.09456895668438392 1105 94-95 0.1474946894324347 1105 96-97 0.2840237452419423 1105 98-99 -0.13929706025071198 1105 100-101 -0.3285425927297858 1106 1 0.48376455533782803 1106 2 0.3429149387163406 1106 3 0.3488465397119995 1106 4 0.24945622806409773 1106 5 -0.020627000229310966 1106 6 0.2866558493261806 1106 7 0.2832761006671305 1106 8 0.25122500736864595 1106 9 0.4622891668397173 1106 10-11 0.49189871613642566 1106 12-13 0.09785840436316562 1106 14-15 0.61365354719495 1106 16-17 0.815384236642771 1106 18-19 0.6830921775011234 1106 20-21 0.6881848180612806 1106 22-23 0.2856285479120544 1106 24-25 0.15644150267637258 1106 26-27 0.4224251957071843 1106 28-29 0.2993385387608498 1106 30-31 0.6207479285661819 1106 32-33 1.1454375218304236 1106 34-35 1.0614768579739646 1106 36-37 0.7649255543706275 1106 38-39 1.1178983246002687 1106 40-41 0.9318718494317508 1106 42-43 1.5211530830735 1106 44-45 1.3258639998055557 1106 46-47 1.5335295068900976 1106 48-49 1.8459098420920057 1106 50-51 1.7705506871161845 1106 52-53 2.259268513788463 1106 54-55 2.1621778818460413 1106 56-57 2.031341333918199 1106 58-59 2.550147454575857 1106 60-61 2.011445053451901 1106 62-63 2.594143542211782 1106 64-65 2.78892738552576 1106 66-67 2.380760797962978 1106 68-69 2.008356456827258 1106 70-71 1.7012631079053975 1106 72-73 2.16352432774962 1106 74-75 2.591612263562407 1106 76-77 2.4969919163043492 1106 78-79 2.588115218609701 1106 80-81 1.8822094864318153 1106 82-83 2.295108056968349 1106 84-85 1.9604170237465013 1106 86-87 2.2309395005724753 1106 88-89 1.5228372807811077 1106 90-91 1.3683096158817172 1106 92-93 1.905431043315616 1106 94-95 1.5454113560990983 1106 96-97 2.0298570785752794 1106 98-99 2.164869606415955 1106 100-101 1.2672907406035492 1107 1 -0.19395653309754834 1107 2 0.17539793191361497 1107 3 0.3662785125011112 1107 4 0.2188439831661384 1107 5 0.13668592494075682 1107 6 0.35340755000645174 1107 7 0.4737522911433203 1107 8 0.42086786451150004 1107 9 0.5515748811254326 1107 10-11 0.3953851106942423 1107 12-13 0.2640999009618099 1107 14-15 0.4763236152221566 1107 16-17 0.40020566521420164 1107 18-19 0.31957176933785547 1107 20-21 0.08997053234699592 1107 22-23 -0.14974560174781004 1107 24-25 0.15559116254031835 1107 26-27 0.15584356305412683 1107 28-29 -0.0025322095384723298 1107 30-31 -0.1819731598691945 1107 32-33 0.12120282795287096 1107 34-35 -0.17406735971310638 1107 36-37 0.34485752715974627 1107 38-39 0.02457349466828873 1107 40-41 -0.06238835464987602 1107 42-43 -0.2651989577428253 1107 44-45 -0.2232431430515831 1107 46-47 0.1615056973662874 1107 48-49 0.5455271890307856 1107 50-51 0.01778708167400822 1107 52-53 0.4250848403190801 1107 54-55 0.6477220995331194 1107 56-57 0.352132150244735 1107 58-59 0.2727239851881009 1107 60-61 -0.2800090281807499 1107 62-63 0.03185612724580267 1107 64-65 -0.5560981246783214 1107 66-67 -0.6196643721050563 1107 68-69 -0.5547812982747828 1107 70-71 -0.5174868920946025 1107 72-73 -0.3602851960599125 1107 74-75 0.0965017193447224 1107 76-77 -1.2644876755323864 1107 78-79 -0.4189000875127462 1107 80-81 -0.536157860506961 1107 82-83 -1.0843562287459356 1107 84-85 -0.6010200510834345 1107 86-87 -0.32008090759079266 1107 88-89 -0.5209552362256993 1107 90-91 -0.4839437854788251 1107 92-93 -0.5971199770925466 1107 94-95 -0.28133184117981 1107 96-97 9.51738133743163E-5 1107 98-99 0.1244784499533722 1107 100-101 -0.03415483762774585 1108 1 0.7093934861554345 1108 2 0.2477891525528193 1108 3 0.3916924516616831 1108 4 0.08907886957353384 1108 5 0.4046874651794994 1108 6 0.3122061637915934 1108 7 0.46566603777404936 1108 8 0.45051746019883154 1108 9 0.5920061479717944 1108 10-11 0.37593959663956156 1108 12-13 0.23425777543234716 1108 14-15 0.8278831069433679 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-0.057173671858173236 2316 52-53 -0.6413725118525591 2316 54-55 -0.07179647712830928 2316 56-57 1.0129118467387102 2316 58-59 0.9051154032938058 2316 60-61 0.7045540278108788 2316 62-63 0.8561627729810155 2316 64-65 -0.5019379990896269 2316 66-67 0.2894146441168246 2316 68-69 -0.39308585086504877 2316 70-71 0.11712849252078428 2316 72-73 0.2532679174932042 2316 74-75 0.5747853404854801 2316 76-77 1.0050047368171704 2316 78-79 0.9238523980968836 2316 80-81 1.1706710248933554 2316 82-83 1.630845236455528 2316 84-85 1.501282408361881 2316 86-87 1.4809395005724753 2316 88-89 1.2359783064221297 2316 90-91 0.5566108979330018 2316 92-93 0.9599182228027949 2316 94-95 0.4452510996888428 2316 96-97 1.0138314375496371 2316 98-99 1.3844208884672398 2316 100-101 1.3746625354753412 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 7.0 15 29.0 16 109.0 17 303.0 18 535.0 19 640.0 20 805.0 21 993.0 22 1035.0 23 813.0 24 772.0 25 631.0 26 720.0 27 846.0 28 1038.0 29 1214.0 30 774.0 31 722.0 32 825.0 33 1018.0 34 1406.0 35 3815.0 36 3842.0 37 7756.0 38 15027.0 39 5775.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29896907216495 22.04823964209298 15.672048239642091 18.98074304609998 2 15.759106362311801 24.64303059737737 41.59689169499757 18.000971345313257 3 16.139873725109275 32.48955803788247 35.708596406022345 15.661971830985916 4 9.53861097620204 25.4356483729966 38.56823700825643 26.45750364254493 5 8.850898494414764 39.8135016998543 38.37979601748421 12.955803788246723 6 20.66440019426906 42.46721709567751 19.176299174356483 17.69208353569694 7 18.105876639145215 38.7508499271491 25.548324429334627 17.594949004371056 8 21.993200582807187 36.68771248178728 23.689169499757163 17.629917435648373 9 23.584264205925205 16.0 24.102962603205437 36.31277319086935 10-11 20.60611947547353 29.424963574550755 30.328314715881493 19.64060223409422 12-13 23.216124332200096 29.423020883924234 30.052452646915977 17.30840213695969 14-15 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36.748907236522584 16.765420106847984 40-41 15.220009713453134 30.153472559494897 37.89800874210782 16.72850898494415 42-43 16.598509941815852 29.998348696927607 37.54966051151541 15.853480849741134 44-45 15.055852355512384 31.524040796503154 37.098591549295776 16.32151529868868 46-47 16.642220905496895 29.97017940922204 34.79489844486105 18.592701240420016 48-49 16.512540555242555 30.29549472539001 34.52101101548384 18.67095370388359 50-51 15.316321673838504 30.140166490203885 35.962466852519206 18.581044983438403 52-53 15.41360686948751 30.23565295100439 37.27804328398803 17.072696895520068 54-55 15.448275862068966 30.589606605148127 35.32880038853812 18.633317144244778 56-57 16.553666828557553 31.090820786789703 33.76202039825158 18.593491986401165 58-59 15.140359397765907 31.50461389023798 35.903836813987375 17.451189898008742 60-61 16.651772705196695 32.64497328800389 32.74502185526955 17.958232151529867 62-63 15.11898980087421 33.96600291403594 33.07042253521127 17.84458474987858 64-65 14.874210781932979 33.99902865468674 32.624575036425455 18.502185526954833 66-67 14.859640602234094 33.80184555609519 32.356483729966 18.982030111704713 68-69 15.146481718926061 33.486808874383186 32.24054862649105 19.126160780199715 70-71 15.25594949004371 33.48324429334629 31.688198154443903 19.5726080621661 72-73 15.66682855755221 32.34968431277319 31.66100048567266 20.32248664400194 74-75 15.99805730937348 32.44681884409908 31.088878096163185 20.466245750364255 76-77 16.270033997085964 32.223409422049535 31.640602234094224 19.865954346770277 78-79 16.561437591063623 32.22826614861583 31.39096648858669 19.81932977173385 80-81 15.891209324915007 32.57503642544925 31.34142787761049 20.192326372025253 82-83 16.051481301602717 32.664400194269064 31.42884895580379 19.85526954832443 84-85 16.030111704711025 32.275862068965516 31.46964545896066 20.2243807673628 86-87 16.268091306459446 32.192326372025256 31.3317144244779 20.207867897037396 88-89 16.936376881981545 31.60174842156386 31.118989800874207 20.34288489558038 90-91 16.406993686255465 32.444876153472556 30.85769791160758 20.2904322486644 92-93 16.089363768819815 32.106847984458476 31.245264691597864 20.55852355512385 94-95 16.31374453618261 31.054881010199125 31.09762020398252 21.533754249635745 96-97 16.71879553181156 30.779990286546866 31.083050024283633 21.418164157357943 98-99 16.011656143759108 30.685769791160755 31.146187469645458 22.156386595434675 100-101 16.472073822243807 30.724623603691114 31.253035454103934 21.55026711996115 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1419.0 1 832.5 2 269.0 3 304.0 4 222.0 5 157.0 6 259.5 7 505.5 8 705.5 9 718.0 10 501.0 11 266.0 12 235.0 13 219.0 14 195.5 15 196.0 16 193.0 17 195.0 18 203.0 19 217.0 20 243.0 21 270.0 22 301.5 23 331.0 24 357.5 25 408.0 26 470.5 27 554.0 28 624.0 29 683.5 30 810.0 31 943.5 32 1078.5 33 1275.5 34 1539.5 35 1718.0 36 1958.5 37 2207.5 38 2377.0 39 2558.0 40 2479.5 41 2285.0 42 2142.5 43 2021.5 44 1838.0 45 1671.5 46 1565.0 47 1347.5 48 1261.5 49 1265.0 50 1301.0 51 1143.0 52 704.5 53 426.5 54 306.0 55 249.5 56 239.5 57 228.0 58 184.5 59 178.5 60 165.0 61 115.5 62 104.5 63 98.0 64 75.0 65 59.0 66 42.5 67 25.5 68 22.5 69 22.0 70 16.0 71 12.0 72 14.0 73 9.0 74 5.0 75 4.0 76 4.5 77 9.5 78 9.0 79 5.5 80 3.0 81 2.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12627489072365225 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 9.713453132588635E-4 44-45 0.0 46-47 9.713453132588635E-4 48-49 0.003885381253035454 50-51 9.713453132588635E-4 52-53 0.001942690626517727 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.001942690626517727 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 51475.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.35405536668286 #Duplication Level Percentage of deduplicated Percentage of total 1 73.52008909449155 33.34434191355027 2 12.396127816328278 11.244293346284605 3 4.998714983294783 6.801359883438563 4 2.6128673006082415 4.740165128703254 5 1.5891373254518975 3.603691112190384 6 0.9252120277563608 2.517727051966974 7 0.7752934121476912 2.46138902379796 8 0.6296581855564122 2.284604176784847 9 0.43690567977383704 1.7833899951432735 >10 2.000342671121391 14.748907236522586 >50 0.05568405722607727 1.6551724137931034 >100 0.03426711213912448 3.498785818358426 >500 0.01713355606956224 6.570179698882953 >1k 0.00856677803478112 4.745993200582807 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 1322 2.568237008256435 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1121 2.177756192326372 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 965 1.8746964545896065 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 938 1.822243807673628 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 871 1.6920835356969404 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 608 1.1811559009227781 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 403 0.7829043224866441 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 283 0.5497814473045167 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 276 0.5361826129188927 RNA PCR Primer, Index 33 (95% over 22bp) GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 242 0.4701311316172899 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 181 0.3516270033997086 RNA PCR Primer, Index 33 (95% over 24bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 163 0.3166585721223895 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 130 0.2525497814473045 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 123 0.23895094706168044 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 96 0.18649830014570182 RNA PCR Primer, Index 33 (96% over 29bp) GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.16318601262748908 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 76 0.14764448761534726 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 74 0.14375910636231182 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 74 0.14375910636231182 RNA PCR Primer, Index 33 (96% over 27bp) GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.13987372510927634 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 59 0.1146187469645459 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 54 0.10490529383195726 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 54 0.10490529383195726 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 53 0.10296260320543954 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 0.10296260320543954 No Hit TTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCC 52 0.1010199125789218 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.713453132588635E-4 0.0 18-19 0.0 0.0 0.0 0.001942690626517727 0.0 20-21 0.0 0.0 0.0 0.0048567265662943174 0.0 22-23 0.0 0.0 0.0 0.015541525012141816 0.001942690626517727 24-25 0.0 0.0 0.0 0.029140359397765908 0.001942690626517727 26-27 0.0 0.0 0.0 0.07576493443419136 0.001942690626517727 28-29 0.0 0.0 0.0 0.192326372025255 0.001942690626517727 30-31 0.0 0.0 0.0 0.35259834871296747 0.001942690626517727 32-33 0.0 0.0 0.0 0.4915007285089849 0.001942690626517727 34-35 0.0 0.0 0.0 0.7139388052452647 0.001942690626517727 36-37 0.0 0.0 0.0 1.017969888295289 0.001942690626517727 38-39 0.0 0.0 0.0 1.3482272948033027 0.001942690626517727 40-41 0.0 0.0 0.0 1.8552695483244293 0.001942690626517727 42-43 0.0 0.0 0.0 2.1670713938805246 0.001942690626517727 44-45 0.0 0.0 0.0 2.419621175327829 0.001942690626517727 46-47 0.0 0.0 0.0 2.6236036911121907 0.001942690626517727 48-49 0.0 0.0 0.0 2.8683827100534236 0.001942690626517727 50-51 0.0 0.0 0.0 3.10247693054881 0.001942690626517727 52-53 0.0 0.0 0.0 3.27343370568237 0.001942690626517727 54-55 0.0 0.0 0.0 3.550267119961146 0.001942690626517727 56-57 0.0 0.0 0.0 3.897037396794561 0.001942690626517727 58-59 0.0 0.0 0.0 4.2204953861097625 0.001942690626517727 60-61 0.0 0.0 0.0 4.454589606605149 0.001942690626517727 62-63 0.0 0.0 0.0 4.69256920835357 0.001942690626517727 64-65 0.0 0.0 0.0 4.936376881981545 0.001942690626517727 66-67 0.0 0.0 0.0 5.200582807187955 0.001942690626517727 68-69 0.0 0.0 0.0 5.523069451189898 0.001942690626517727 70-71 0.0 0.0 0.0 5.89800874210782 0.001942690626517727 72-73 0.0 0.0 0.0 6.23895094706168 0.001942690626517727 74-75 0.0 0.0 0.0 6.611947547353084 0.001942690626517727 76-77 0.0 0.0 0.0 6.976202039825157 0.001942690626517727 78-79 0.0 0.0 0.0 7.381253035454105 0.001942690626517727 80-81 0.0 0.0 0.0 7.788246721709568 0.001942690626517727 82-83 0.0 0.0 0.0 8.212724623603691 0.001942690626517727 84-85 0.0 0.0 0.0 8.612918892666343 0.001942690626517727 86-87 0.0 0.0 0.0 9.04225352112676 0.001942690626517727 88-89 0.0 0.0 0.0 9.490043710539096 0.001942690626517727 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTCTG 15 6.582006E-4 94.99904 9 TAGAGGA 20 0.0020628835 71.24927 4 TTAATCT 25 0.0049943305 56.999416 4 ATCTAAC 25 0.0049943305 56.999416 8 TCTAACA 25 0.0049943305 56.999416 9 CGCAGAA 60 2.293018E-8 55.416107 9 GGTATCA 575 0.0 46.305397 1 AGTGGCT 50 0.0016015881 37.99961 8 CTACTGA 60 0.0038978383 31.69715 1 ACTATAC 90 2.3689563E-5 31.666342 3 GTATCAA 900 0.0 30.11229 1 CATGGGG 130 2.9429975E-7 29.23047 4 CTTGAAA 295 0.0 28.982754 56-57 GCTTGAA 310 0.0 27.580362 56-57 TGCTTGA 320 0.0 27.460655 54-55 CAACGCA 990 0.0 27.348206 6 TCAACGC 995 0.0 27.210777 5 GGTTTCC 35 0.008357149 27.142578 70-71 TATAGTG 70 0.008318992 27.142578 5 AACGCAG 990 0.0 26.868412 7 >>END_MODULE