FastQCFastQC Report
Fri 10 Feb 2017
SRR2031775_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031775_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307374
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44500.34037696940584716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42000.32125466775383327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24680.18877536190868108No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.059.00481
GTATCAA38650.039.9417841
TCAACGC43750.033.8742874
ATCAACG44000.033.5738643
CAACGCA44850.033.2552955
AACGCAG45800.032.7729266
TATCAAC47300.031.7336162
ACGCAGA50900.029.2092327
CGCAGAG52000.028.5913498
TCCGCGC856.407654E-427.9411758
CGCCGTA1450.026.20689694-95
GCAGAGT59300.024.9915689
GTACATG24250.023.701031
GAGTACT45100.023.6973412-13
CAGAGTA57450.022.94386310-11
AGAGTAC52600.021.94391610-11
GTACTTT48950.021.68794614-15
TACATGG25550.021.5655572
AGTACTT48400.019.67716812-13
ACATGGG26650.019.0712933
Produced by FastQC (version 0.11.5)