##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031775_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1307374 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46000149918845 34.0 31.0 34.0 31.0 34.0 2 32.59485732468291 34.0 31.0 34.0 31.0 34.0 3 32.668179878137394 34.0 31.0 34.0 31.0 34.0 4 35.88138053839223 37.0 37.0 37.0 35.0 37.0 5 35.904391551308194 37.0 37.0 37.0 35.0 37.0 6 35.98096107158319 37.0 37.0 37.0 35.0 37.0 7 35.99971087079902 37.0 37.0 37.0 35.0 37.0 8 35.998418968099415 37.0 37.0 37.0 35.0 37.0 9 37.81638842442943 39.0 39.0 39.0 35.0 39.0 10-11 37.847253731525946 39.0 39.0 39.0 35.0 39.0 12-13 37.82312635863953 39.0 39.0 39.0 35.0 39.0 14-15 39.37047011796166 41.0 40.0 41.0 37.0 41.0 16-17 39.30413676576099 41.0 40.0 41.0 36.0 41.0 18-19 39.312840090134884 41.0 40.0 41.0 36.5 41.0 20-21 39.261087875389904 41.0 40.0 41.0 36.5 41.0 22-23 39.17326641037683 41.0 39.5 41.0 36.0 41.0 24-25 39.13544938173774 41.0 39.0 41.0 36.0 41.0 26-27 39.01742194658912 41.0 39.0 41.0 35.5 41.0 28-29 38.89064873555692 41.0 39.0 41.0 35.0 41.0 30-31 38.73351504619183 40.5 39.0 41.0 35.0 41.0 32-33 38.59642152895805 40.0 38.0 41.0 35.0 41.0 34-35 38.41058411747518 40.0 38.0 41.0 34.5 41.0 36-37 38.28899840443515 40.0 38.0 41.0 34.0 41.0 38-39 38.10242516678471 40.0 38.0 41.0 33.5 41.0 40-41 38.0068664360772 40.0 38.0 41.0 33.0 41.0 42-43 37.839469807415476 40.0 38.0 41.0 33.0 41.0 44-45 37.988949986767366 40.0 38.0 41.0 33.0 41.0 46-47 37.85399090084398 40.0 38.0 41.0 33.0 41.0 48-49 37.680801362119794 40.0 38.0 41.0 32.5 41.0 50-51 36.624851419716165 39.0 36.5 40.0 31.0 40.5 52-53 36.65876749881824 39.5 37.0 40.0 30.5 40.5 54-55 37.4610172758522 40.0 38.0 41.0 32.0 41.0 56-57 37.470102281367076 40.0 37.5 41.0 32.0 41.0 58-59 37.358072747354626 40.0 37.0 41.0 32.0 41.0 60-61 37.16948516644816 40.0 36.5 41.0 31.0 41.0 62-63 36.916851260618614 40.0 36.0 41.0 31.0 41.0 64-65 36.618230896438206 39.0 35.5 41.0 30.5 41.0 66-67 36.35718623745004 39.0 35.0 41.0 30.0 41.0 68-69 36.039943046136756 38.5 35.0 41.0 30.0 41.0 70-71 35.6858377939289 37.0 35.0 40.0 29.5 41.0 72-73 35.28978433103305 37.0 35.0 39.5 29.0 41.0 74-75 34.92849941944692 36.5 35.0 39.0 29.0 41.0 76-77 34.54929385164459 36.0 35.0 39.0 29.0 40.5 78-79 34.161287053283914 35.5 34.5 37.5 28.5 39.5 80-81 33.799795620839944 35.0 34.0 37.0 28.0 39.0 82-83 33.50352271041034 35.0 34.0 37.0 27.0 39.0 84-85 33.18736375360073 35.0 34.0 36.0 27.0 37.0 86-87 32.917199668954716 35.0 34.0 36.0 26.0 37.0 88-89 32.70685090876826 35.0 34.0 35.5 26.0 37.0 90-91 32.528135407312675 35.0 34.0 35.0 26.0 36.0 92-93 32.39307956254293 35.0 34.0 35.0 25.0 36.0 94-95 32.231396677614825 35.0 34.0 35.0 25.0 36.0 96-97 32.128101446104935 35.0 34.0 35.0 25.0 36.0 98-99 32.0157514223168 35.0 33.5 35.0 24.5 35.0 100-101 30.521754295251398 34.0 30.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.278282495816228 1101 2 0.26923152969662567 1101 3 0.30972026167951583 1101 4 0.2335385094865714 1101 5 0.2565757647435447 1101 6 0.19258782633247762 1101 7 0.2127337158514564 1101 8 0.3303547651366543 1101 9 0.30019394874717165 1101 10-11 0.3165089680063673 1101 12-13 0.29290198744666895 1101 14-15 0.2194464577451143 1101 16-17 0.43814262339216725 1101 18-19 0.49767740272393013 1101 20-21 0.3956518403860656 1101 22-23 0.4836168184470466 1101 24-25 0.3451281317098136 1101 26-27 0.41954572126194023 1101 28-29 0.4850589851165381 1101 30-31 0.5028240129388593 1101 32-33 0.2655024922606657 1101 34-35 0.371607800946542 1101 36-37 0.42652940071915424 1101 38-39 0.5835512114153971 1101 40-41 0.4786562563980681 1101 42-43 0.3407387154077952 1101 44-45 0.543161477242684 1101 46-47 0.4923048566762773 1101 48-49 0.46603313543086955 1101 50-51 0.4471952222882365 1101 52-53 0.5475541653583491 1101 54-55 0.6053069766064354 1101 56-57 0.5673238655655979 1101 58-59 0.45256621802089825 1101 60-61 0.4844719132733033 1101 62-63 0.31913889708544474 1101 64-65 0.5505695764692646 1101 66-67 0.5214558410468584 1101 68-69 0.5430201713742804 1101 70-71 0.4332613748301526 1101 72-73 0.5856941323885536 1101 74-75 0.5735261419158846 1101 76-77 0.4279657532452106 1101 78-79 0.3522736810549958 1101 80-81 0.47749775970819996 1101 82-83 0.4408597063602926 1101 84-85 0.3678569085469192 1101 86-87 0.3047942331829674 1101 88-89 0.4937542547283158 1101 90-91 0.5405334095868497 1101 92-93 0.3917012781708209 1101 94-95 0.4209147277737273 1101 96-97 0.34261705460944114 1101 98-99 0.37748221169465523 1101 100-101 0.442389261491126 1102 1 0.15866410677794107 1102 2 0.2266844686132643 1102 3 0.24698175482844675 1102 4 0.24383432201058497 1102 5 0.22223397962380886 1102 6 0.24666325212323414 1102 7 0.22810380164989397 1102 8 0.3633903650854293 1102 9 0.42469772643958237 1102 10-11 0.39805910246661114 1102 12-13 0.415757026504167 1102 14-15 0.4534088729071115 1102 16-17 0.4283286267216653 1102 18-19 0.4726277804931698 1102 20-21 0.5098502012517372 1102 22-23 0.4458510994049192 1102 24-25 0.5674389269460818 1102 26-27 0.5310372694755472 1102 28-29 0.6337351920572445 1102 30-31 0.5515914607554819 1102 32-33 0.5706039777261509 1102 34-35 0.5219455522591332 1102 36-37 0.5100356115015856 1102 38-39 0.5619751453377972 1102 40-41 0.46251142351293595 1102 42-43 0.4880767228351246 1102 44-45 0.4776233161465129 1102 46-47 0.6519155622723858 1102 48-49 0.6901463381456878 1102 50-51 0.6820380318669308 1102 52-53 0.7574818129444694 1102 54-55 0.7389412447585642 1102 56-57 0.6653309727496151 1102 58-59 0.604184543030506 1102 60-61 0.7114984211488249 1102 62-63 0.6008923226200835 1102 64-65 0.700489540613205 1102 66-67 0.7020135308919038 1102 68-69 0.7376353589783307 1102 70-71 0.6560475830514392 1102 72-73 0.7229508233861281 1102 74-75 0.686748065337575 1102 76-77 0.6711563026113296 1102 78-79 0.6098642816440645 1102 80-81 0.6422099507697965 1102 82-83 0.6840214428182279 1102 84-85 0.7737747597442493 1102 86-87 0.7678464805898031 1102 88-89 0.6934421148896703 1102 90-91 0.6534656032095896 1102 92-93 0.5239893432239668 1102 94-95 0.6743786796241551 1102 96-97 0.6451958098918098 1102 98-99 0.6435101922299324 1102 100-101 0.7450384483268273 1103 1 0.22468534716988842 1103 2 0.09299321707303676 1103 3 0.2292970749012042 1103 4 0.1697980607889633 1103 5 0.09142367627345749 1103 6 0.20909357542312534 1103 7 0.17610004644247823 1103 8 0.20315155736378188 1103 9 0.2324193716898293 1103 10-11 0.16681900389241378 1103 12-13 0.21866731225198066 1103 14-15 0.2898651892833328 1103 16-17 0.3052223660867952 1103 18-19 0.26284810555331006 1103 20-21 0.4001101320151008 1103 22-23 0.36775158640838157 1103 24-25 0.2802799760571588 1103 26-27 0.2976628635610865 1103 28-29 0.430959859075962 1103 30-31 0.3822609438277169 1103 32-33 0.2469476378798774 1103 34-35 0.34163105363799673 1103 36-37 0.32634142620557327 1103 38-39 0.28510047538532035 1103 40-41 0.31879552842404735 1103 42-43 0.3573192270890715 1103 44-45 0.428663332575546 1103 46-47 0.4443347373002524 1103 48-49 0.41474294672399026 1103 50-51 0.3393372162828143 1103 52-53 0.5186652900318336 1103 54-55 0.5191163899794802 1103 56-57 0.4787485048742397 1103 58-59 0.47560682783952046 1103 60-61 0.5579559110273706 1103 62-63 0.4339513497866605 1103 64-65 0.5112505568662087 1103 66-67 0.6007652178014808 1103 68-69 0.5591749940191093 1103 70-71 0.4513829113387118 1103 72-73 0.45098872751098895 1103 74-75 0.4523893843541771 1103 76-77 0.33955975270814065 1103 78-79 0.45118916576070944 1103 80-81 0.4577955284263098 1103 82-83 0.3712420025788319 1103 84-85 0.2729313049534241 1103 86-87 0.275301154062781 1103 88-89 0.19116258663760277 1103 90-91 0.19411865724937627 1103 92-93 0.16071996576694403 1103 94-95 0.2378592874983596 1103 96-97 0.3144925886279495 1103 98-99 0.38746225466025663 1103 100-101 0.47336386051951607 1104 1 0.29217446182881446 1104 2 0.3074344362312402 1104 3 0.15922071975511898 1104 4 0.27742095471210604 1104 5 0.21245604833126208 1104 6 0.23266270522687194 1104 7 0.21167460314090647 1104 8 0.29616600804845916 1104 9 0.29875684881483977 1104 10-11 0.2670824606981128 1104 12-13 0.276564706080471 1104 14-15 0.3805921407567965 1104 16-17 0.3402211040182692 1104 18-19 0.4185242587684286 1104 20-21 0.41818137578363235 1104 22-23 0.46818857763700805 1104 24-25 0.3833751991277694 1104 26-27 0.4616134535781313 1104 28-29 0.5620572276630753 1104 30-31 0.503498402021691 1104 32-33 0.39106797611424327 1104 34-35 0.40339353234058706 1104 36-37 0.5709145315251902 1104 38-39 0.5850073978312125 1104 40-41 0.5484446733726145 1104 42-43 0.501810297954215 1104 44-45 0.4661467679260909 1104 46-47 0.5997348130243623 1104 48-49 0.5958182291118277 1104 50-51 0.46872290442568243 1104 52-53 0.5248652016870423 1104 54-55 0.63072268770145 1104 56-57 0.6164244056756516 1104 58-59 0.5121515997206316 1104 60-61 0.5247556207516055 1104 62-63 0.5746009995626409 1104 64-65 0.6510483103842262 1104 66-67 0.5539582511831966 1104 68-69 0.6153212677617717 1104 70-71 0.5536577898646158 1104 72-73 0.5965288928419596 1104 74-75 0.46410295790121836 1104 76-77 0.49389495810979156 1104 78-79 0.4899583334265145 1104 80-81 0.45905759695663306 1104 82-83 0.43008594780506115 1104 84-85 0.48599213665658425 1104 86-87 0.492171855141855 1104 88-89 0.4744338682681928 1104 90-91 0.4726098543213766 1104 92-93 0.49656428961372256 1104 94-95 0.6150048039999021 1104 96-97 0.5922738721516438 1104 98-99 0.45266387010797615 1104 100-101 0.6028126520889003 1105 1 0.16153492017506466 1105 2 0.11544044947450516 1105 3 0.14634379629575278 1105 4 0.22916127575858525 1105 5 0.17502504820435405 1105 6 0.28302688848687296 1105 7 0.23536057995930548 1105 8 0.2955275261699626 1105 9 0.35651590825776225 1105 10-11 0.16344985206788465 1105 12-13 0.26344442682314906 1105 14-15 0.29555400847329594 1105 16-17 0.4007767925908823 1105 18-19 0.4371812095202827 1105 20-21 0.43596662868394986 1105 22-23 0.4541046879216637 1105 24-25 0.3864979381103595 1105 26-27 0.4565157383750673 1105 28-29 0.468503932089142 1105 30-31 0.479262912111146 1105 32-33 0.4509070080291764 1105 34-35 0.4599040849545091 1105 36-37 0.44735618566426893 1105 38-39 0.5494552091336473 1105 40-41 0.4212036085209121 1105 42-43 0.5805807100759495 1105 44-45 0.5660204453331232 1105 46-47 0.6222903629103413 1105 48-49 0.6434158756257489 1105 50-51 0.45520390108542585 1105 52-53 0.5029363583990119 1105 54-55 0.511030558953145 1105 56-57 0.583871642606077 1105 58-59 0.5555003325041881 1105 60-61 0.4004138916432467 1105 62-63 0.5826307596216793 1105 64-65 0.6886474353500418 1105 66-67 0.5761363379094462 1105 68-69 0.5921010687072013 1105 70-71 0.5011034044230556 1105 72-73 0.5461964374212158 1105 74-75 0.4368278101541634 1105 76-77 0.4301674668856492 1105 78-79 0.5738594969550661 1105 80-81 0.5803678455066361 1105 82-83 0.4574664188947821 1105 84-85 0.4488146321525406 1105 86-87 0.5831574853744925 1105 88-89 0.5899333429598457 1105 90-91 0.598680914214377 1105 92-93 0.569563505903389 1105 94-95 0.5782463032286174 1105 96-97 0.7060809773559313 1105 98-99 0.6607749450210036 1105 100-101 0.6099108566202887 1106 1 0.1831980980060095 1106 2 0.1456400607185273 1106 3 0.15489019781090008 1106 4 0.16776524306321505 1106 5 0.32057553664134986 1106 6 0.15890283545656558 1106 7 0.22381377972006788 1106 8 0.11603565017315276 1106 9 0.2380475071413386 1106 10-11 0.20535762220345077 1106 12-13 0.14755527211820407 1106 14-15 0.1286076119421935 1106 16-17 0.20560384601991188 1106 18-19 0.16443150856334654 1106 20-21 0.19412925826927108 1106 22-23 0.19896239326456566 1106 24-25 0.1841535651039834 1106 26-27 0.23823299315975532 1106 28-29 0.25068968767127586 1106 30-31 0.2732334563695247 1106 32-33 0.23546143386650442 1106 34-35 0.2409765281363221 1106 36-37 0.16492185053667896 1106 38-39 0.2497835334956875 1106 40-41 0.29086257482872924 1106 42-43 0.1705260101158288 1106 44-45 0.1540761670237103 1106 46-47 0.17209785393905008 1106 48-49 0.08588372849656878 1106 50-51 -0.04427090453658167 1106 52-53 -0.03319115854676369 1106 54-55 0.011576935548035294 1106 56-57 0.04140005169698924 1106 58-59 0.0792256614515523 1106 60-61 0.1280280264119824 1106 62-63 0.20096770639201367 1106 64-65 0.2502359770167075 1106 66-67 0.11569911203226013 1106 68-69 0.09498185020640193 1106 70-71 0.04476057647249121 1106 72-73 0.08922686505279387 1106 74-75 0.1668398964779243 1106 76-77 0.04812998682185565 1106 78-79 0.06459975313528332 1106 80-81 0.07295282357681288 1106 82-83 0.1463569691340112 1106 84-85 0.19114043956881233 1106 86-87 0.03330700690559496 1106 88-89 0.0693672793633553 1106 90-91 0.10233484663064729 1106 92-93 0.18400853182046006 1106 94-95 0.2808685370802948 1106 96-97 0.1439828164707606 1106 98-99 0.11512477556326672 1106 100-101 0.21120484087068547 1107 1 0.07957767117674308 1107 2 0.1762993594990121 1107 3 0.12102967951821597 1107 4 0.13617225525833732 1107 5 0.2901067866413527 1107 6 0.20255756106632106 1107 7 0.15523527362250888 1107 8 0.24711415627071176 1107 9 0.18457951933645944 1107 10-11 0.20306150939857304 1107 12-13 0.15487234528892913 1107 14-15 0.12238619425927055 1107 16-17 0.25617549236137194 1107 18-19 0.20623791100236843 1107 20-21 0.24837849607415308 1107 22-23 0.1570607164352964 1107 24-25 0.15176979986007666 1107 26-27 0.1783054016963419 1107 28-29 0.20890234011030628 1107 30-31 0.21784092588171688 1107 32-33 0.17608643386650158 1107 34-35 0.23540297630704998 1107 36-37 0.30043061578057717 1107 38-39 0.28205296947129455 1107 40-41 0.2542676815360494 1107 42-43 0.43311175706704574 1107 44-45 0.42903805726761135 1107 46-47 0.31881088747562814 1107 48-49 0.41513296630144225 1107 50-51 0.37828054363414765 1107 52-53 0.36436028962396705 1107 54-55 0.2734767068895039 1107 56-57 0.367200356575033 1107 58-59 0.2826745943783848 1107 60-61 0.3619838190949096 1107 62-63 0.23950619724567446 1107 64-65 0.2962439800654906 1107 66-67 0.2693862309346997 1107 68-69 0.25893002093810935 1107 70-71 0.2819461709846891 1107 72-73 0.37079127054059313 1107 74-75 0.3307975946486508 1107 76-77 0.23341962096819913 1107 78-79 0.30458641472065295 1107 80-81 0.29499931747925245 1107 82-83 0.2567228227925469 1107 84-85 0.3305649822517367 1107 86-87 0.37027347032022107 1107 88-89 0.4160993677779885 1107 90-91 0.3394251667525978 1107 92-93 0.36222880621070175 1107 94-95 0.3976844669583457 1107 96-97 0.415562465861008 1107 98-99 0.49986181824619536 1107 100-101 0.5213515634316614 1108 1 0.21980818219341813 1108 2 0.18986933414458917 1108 3 0.1543743211637576 1108 4 0.22443846780699062 1108 5 0.3012250150454534 1108 6 0.2318267465107411 1108 7 0.24985125226765348 1108 8 0.23206442945572547 1108 9 0.31310927507691133 1108 10-11 0.30155374683742053 1108 12-13 0.20350329554425883 1108 14-15 0.26955346205200925 1108 16-17 0.2994705187871105 1108 18-19 0.30956556786055955 1108 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-0.471971019098099 2316 62-63 -0.6802209676305893 2316 64-65 -0.5933725198525579 2316 66-67 -0.39008342652914507 2316 68-69 -0.33338940819310636 2316 70-71 -0.30755239122756706 2316 72-73 -0.45938984042980024 2316 74-75 -0.8832199977645914 2316 76-77 -0.9039698910034346 2316 78-79 -0.5244558268127832 2316 80-81 -0.4382905904427332 2316 82-83 -0.6001858683736216 2316 84-85 -0.33993345704084277 2316 86-87 -0.18801629792030639 2316 88-89 -0.2690325660092796 2316 90-91 -0.2951374254371544 2316 92-93 -0.46718219435548036 2316 94-95 -0.5367148376142694 2316 96-97 -0.45960678149808487 2316 98-99 -0.5502945318588459 2316 100-101 -0.10999509613298031 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 16.0 7 281.0 8 738.0 9 633.0 10 685.0 11 1024.0 12 1349.0 13 1709.0 14 1882.0 15 2057.0 16 2344.0 17 2996.0 18 3991.0 19 5146.0 20 6752.0 21 8714.0 22 10478.0 23 10536.0 24 9539.0 25 8782.0 26 8438.0 27 8719.0 28 9598.0 29 11180.0 30 13177.0 31 16034.0 32 20308.0 33 27221.0 34 39160.0 35 63275.0 36 119178.0 37 270918.0 38 455675.0 39 164637.0 40 204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.25439698204186 20.008199642948384 14.11417084935145 23.623232525658306 2 15.07579315482792 21.27853238629497 40.23424054631651 23.411433912560597 3 17.10367500042069 25.80845266924384 32.185663780983866 24.902208549351602 4 12.095161751725215 18.432216029996006 39.414199762271544 30.058422456007232 5 12.701109246474228 38.16184198247785 36.25083564458219 12.886213126465723 6 29.685384595379745 38.902945905303305 18.183855576139653 13.227813923177301 7 25.243503389236743 34.03004801992391 23.40324956745354 17.32319902338581 8 24.51777379693951 35.600906856033546 21.542955573539018 18.338363773487924 9 25.481843757027445 15.419076714084875 21.604376406445287 37.49470312244239 10-11 23.180015817967927 26.69553624288077 30.576789809190025 19.54765812996128 12-13 24.137048771047915 25.169729549463277 29.634710496001908 21.058511183486896 14-15 21.560968781695212 26.801320815619707 27.519783933289172 24.117926469395904 16-17 20.80414632691181 29.387038445005025 28.352904371664117 21.45591085641905 18-19 20.64474282034062 28.665362780658022 30.297183514434277 20.392710884567077 20-21 21.493543546070214 27.94085701566652 30.31485252116074 20.25074691710253 22-23 21.26704516119163 27.90031150265131 29.84792016047454 20.984723175682525 24-25 21.194464926585546 27.9155053037697 30.033685898122343 20.856343871522412 26-27 21.114577771930605 28.078652321370935 29.904717395328344 20.90205251137012 28-29 21.086467988502143 28.05394630763653 30.04867008216471 20.810915621696623 30-31 21.299949364145228 27.916112757328815 30.08928585087358 20.694652027652378 32-33 21.09197521137792 28.020979459588457 29.96430248727602 20.9227428417576 34-35 21.42619479965182 28.18600492284534 29.66079331545526 20.727006962047586 36-37 21.378350800918483 28.06010368876848 29.697049199387475 20.864496310925563 38-39 21.261475293221373 28.133074391872565 29.733075615699867 20.872374699206194 40-41 21.432122713163945 28.05887986146275 29.69777584685025 20.811221578523053 42-43 21.272030803733287 28.198472663522452 29.603617633515732 20.925878899228533 44-45 21.410093821660826 28.35141283213526 29.25991338362244 20.97857996258148 46-47 21.35850185180369 28.287506100014227 29.154664235329754 21.199327812852328 48-49 21.404204152752005 28.416313923942194 29.029527893318974 21.14995402998683 50-51 21.39200412429802 28.63136332832074 28.863890516409228 21.112742030972008 52-53 21.368598427075955 28.6850969959629 28.79191417299105 21.154390403970098 54-55 21.431702022527602 28.615836019379305 28.709305829854348 21.243156128238745 56-57 21.416518915015903 28.637941400089034 28.739672044877747 21.205867640017317 58-59 21.379995318860555 28.543247762308262 28.86683535086364 21.209921567967545 60-61 21.3718874629601 28.529288482102288 28.895557047944965 21.203267006992643 62-63 21.191678892191522 28.604133170768275 28.89769874572999 21.306489191310217 64-65 21.28526343647648 28.491732281657733 28.803464043188864 21.41954023867692 66-67 21.29673681746769 28.524813863515718 28.68284056436796 21.495608754648632 68-69 21.24701883317245 28.63885927056833 28.6661276727241 21.447994223535115 70-71 21.27417250151831 28.386597867174963 28.723035642440497 21.616193988866232 72-73 21.168732130209104 28.44602998070942 28.743381771398234 21.641856117683233 74-75 21.205026258744628 28.468173606022457 28.6718261186164 21.654974016616517 76-77 21.18425943915054 28.42549262873516 28.686588535491758 21.703659396622545 78-79 21.12849880753327 28.396082528794363 28.61266171730507 21.8627569463673 80-81 21.1439878718714 28.458115275353496 28.56665345953033 21.83124339324478 82-83 21.077289283709174 28.412068772975445 28.63270188943638 21.877940053878998 84-85 21.12272387243436 28.301694847840018 28.682190406111797 21.893390873613825 86-87 21.12945492261587 28.23920316604124 28.662035500170568 21.969306411172322 88-89 21.10555204555085 28.171816175019543 28.702651268879446 22.019980510550155 90-91 21.11568686542642 28.14014964348381 28.75669089334804 21.987472597741732 92-93 21.06658079478405 27.969043288301588 28.866644127847117 22.097731789067247 94-95 20.963894034912734 27.966060209243874 28.82572240231181 22.244323353531584 96-97 20.950737891376146 27.89924688727174 28.86010430068213 22.289910920669982 98-99 21.014759357307092 27.779503034326826 28.8370045602865 22.368733048079587 100-101 21.008334263952015 27.65987391519183 28.898769594622504 22.433022226233657 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 483.0 1 320.5 2 204.5 3 260.0 4 229.5 5 216.0 6 336.5 7 690.0 8 1167.0 9 1476.0 10 1345.5 11 1141.5 12 1215.5 13 1353.0 14 1472.5 15 1603.0 16 1699.0 17 1794.0 18 1954.5 19 2214.0 20 2619.5 21 3164.5 22 3901.0 23 4870.5 24 6118.5 25 7609.5 26 9665.5 27 12237.5 28 14918.0 29 17750.0 30 20869.0 31 23859.5 32 26788.5 33 29871.5 34 32867.5 35 35047.0 36 37265.5 37 40204.5 38 42822.5 39 44432.5 40 45961.0 41 47634.0 42 48767.0 43 49928.5 44 51086.0 45 51864.5 46 51729.5 47 51207.5 48 50636.5 49 49475.5 50 47245.5 51 44268.0 52 41353.0 53 38219.5 54 34715.0 55 30893.0 56 26391.0 57 22157.5 58 18979.0 59 15990.0 60 12780.0 61 9881.5 62 7539.0 63 5489.5 64 3906.0 65 2913.5 66 2151.5 67 1614.0 68 1228.5 69 915.0 70 655.5 71 485.5 72 391.0 73 264.0 74 187.5 75 168.0 76 127.5 77 86.0 78 60.5 79 36.5 80 28.5 81 26.5 82 20.0 83 14.0 84 11.0 85 12.5 86 15.5 87 12.5 88 9.0 89 5.5 90 5.0 91 5.0 92 3.0 93 2.0 94 1.0 95 0.5 96 1.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.1473380991208331E-4 24-25 1.5297841321611108E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1307374.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.44902131189376 #Duplication Level Percentage of deduplicated Percentage of total 1 74.39195819501842 49.43272815534287 2 15.829644081083178 21.03728713807178 3 5.135552231353067 10.237572590085705 4 2.1102658975115394 5.609004143900275 5 1.0030800374511466 3.332684339306322 6 0.5532059687222037 2.205599712329313 7 0.31221667771435585 1.4522544869958929 8 0.19274653985776638 1.0246255147841998 9 0.11798458981619878 0.7055964470854472 >10 0.3448922424359865 3.3971048609800567 >50 0.004642188037550357 0.20524094365619092 >100 0.0033493752441034284 0.4659201936075343 >500 1.1549393861960531E-4 0.04113510125158584 >1k 3.464818158588159E-4 0.8532463726028571 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4450 0.34037696940584716 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4200 0.32125466775383327 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2468 0.18877536190868108 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.648920660805554E-5 0.0 5 0.0 0.0 0.0 7.648920660805554E-5 0.0 6 0.0 0.0 0.0 7.648920660805554E-5 0.0 7 0.0 0.0 0.0 7.648920660805554E-5 0.0 8 0.0 0.0 0.0 7.648920660805554E-5 0.0 9 0.0 0.0 0.0 7.648920660805554E-5 7.648920660805554E-5 10-11 0.0 0.0 0.0 7.648920660805554E-5 7.648920660805554E-5 12-13 0.0 0.0 0.0 7.648920660805554E-5 7.648920660805554E-5 14-15 0.0 0.0 0.0 7.648920660805554E-5 7.648920660805554E-5 16-17 0.0 0.0 0.0 1.1473380991208331E-4 7.648920660805554E-5 18-19 0.0 0.0 0.0 1.5297841321611108E-4 7.648920660805554E-5 20-21 0.0 0.0 0.0 1.9122301652013885E-4 7.648920660805554E-5 22-23 0.0 0.0 0.0 4.2069063634430547E-4 7.648920660805554E-5 24-25 0.0 0.0 0.0 9.943596859047221E-4 7.648920660805554E-5 26-27 0.0 0.0 0.0 0.0016062733387691662 7.648920660805554E-5 28-29 0.0 0.0 0.0 0.005927913512124304 7.648920660805554E-5 30-31 0.0 0.0 0.0 0.014609438462138608 7.648920660805554E-5 32-33 0.0 0.0 0.0 0.02891292009784499 1.5297841321611108E-4 34-35 0.0 0.0 0.0 0.05434558129502346 1.9122301652013885E-4 36-37 0.0 0.0 0.0 0.09828863049135136 2.2946761982416662E-4 38-39 0.0 0.0 0.0 0.1557702692573051 2.2946761982416662E-4 40-41 0.0 0.0 0.0 0.21627323168427703 3.0595682643222216E-4 42-43 0.0 0.0 0.0 0.2710777482189488 3.0595682643222216E-4 44-45 0.0 0.0 0.0 0.34282462401730496 3.0595682643222216E-4 46-47 0.0 0.0 0.0 0.43663863592208507 3.0595682643222216E-4 48-49 0.0 0.0 0.0 0.5383310361074949 3.0595682643222216E-4 50-51 0.0 0.0 0.0 0.6350898824666851 3.0595682643222216E-4 52-53 0.0 0.0 0.0 0.7302042108838022 3.0595682643222216E-4 54-55 0.0 0.0 0.0 0.8440201503165888 3.0595682643222216E-4 56-57 0.0 0.0 0.0 0.9858693839712278 3.0595682643222216E-4 58-59 0.0 0.0 0.0 1.1497857537322909 3.824460330402777E-4 60-61 0.0 0.0 0.0 1.3128607422206653 3.824460330402777E-4 62-63 0.0 0.0 0.0 1.4739470113372302 3.824460330402777E-4 64-65 0.0 0.0 0.0 1.6613838121302704 3.824460330402777E-4 66-67 0.0 0.0 0.0 1.8869504824174261 3.824460330402777E-4 68-69 0.0 0.0 0.0 2.144872087099789 3.824460330402777E-4 70-71 0.0 0.0 0.0 2.4186269575500203 3.824460330402777E-4 72-73 0.0 0.0 0.0 2.6835090800337165 3.824460330402777E-4 74-75 0.0 0.0 0.0 2.978872151350723 3.824460330402777E-4 76-77 0.0 0.0 0.0 3.2948108192452965 3.824460330402777E-4 78-79 0.0 0.0 0.0 3.652397860137956 3.824460330402777E-4 80-81 0.0 0.0 0.0 4.040924785103574 3.824460330402777E-4 82-83 0.0 0.0 0.0 4.459091277629813 3.824460330402777E-4 84-85 0.0 0.0 0.0 4.882420791602097 3.824460330402777E-4 86-87 0.0 0.0 0.0 5.337110880283683 3.824460330402777E-4 88-89 0.0 0.0 0.0 5.878654463068717 3.824460330402777E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2085 0.0 59.0048 1 GTATCAA 3865 0.0 39.941784 1 TCAACGC 4375 0.0 33.874287 4 ATCAACG 4400 0.0 33.573864 3 CAACGCA 4485 0.0 33.255295 5 AACGCAG 4580 0.0 32.772926 6 TATCAAC 4730 0.0 31.733616 2 ACGCAGA 5090 0.0 29.209232 7 CGCAGAG 5200 0.0 28.591349 8 TCCGCGC 85 6.407654E-4 27.941175 8 CGCCGTA 145 0.0 26.206896 94-95 GCAGAGT 5930 0.0 24.991568 9 GTACATG 2425 0.0 23.70103 1 GAGTACT 4510 0.0 23.69734 12-13 CAGAGTA 5745 0.0 22.943863 10-11 AGAGTAC 5260 0.0 21.943916 10-11 GTACTTT 4895 0.0 21.687946 14-15 TACATGG 2555 0.0 21.565557 2 AGTACTT 4840 0.0 19.677168 12-13 ACATGGG 2665 0.0 19.071293 3 >>END_MODULE