FastQCFastQC Report
Fri 10 Feb 2017
SRR2031775_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031775_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307374
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46350.35452747262833745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44600.3411418614719277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26350.20154905941222634No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT23530.17997910314875468No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.059.321
GTATCAA37150.039.6950841
ATCAACG43850.033.146483
TCAACGC43600.033.1186524
CAACGCA44450.032.2716185
AACGCAG45300.031.666086
TATCAAC47050.031.0940062
TACCGGT804.494463E-429.686957
ACGCAGA50250.028.2631577
CGCAGAG50250.027.8850568
CGTACAC1151.282046E-424.782153
GCAGAGT57950.023.6880869
GAGTACT44000.023.04786912-13
CAGAGTA55100.022.41337810-11
AGAGTAC50800.021.83276610-11
GTACATG22700.021.1655391
GTATAGC5750.020.6826421
CCGTACC1150.00372394720.651799
GTACTTT48950.020.5716314-15
AGTACTT48050.019.32586512-13
Produced by FastQC (version 0.11.5)