FastQCFastQC Report
Fri 10 Feb 2017
SRR2031774_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031774_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40017
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9632.4064772471699527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5521.3794137491566085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5391.3469275557887899No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5281.3194392383237123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.6947047504810455No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.5172801559337281No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.47229927280905615No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.40732688607341877No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.3073693680185921No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG980.24489591923432544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.24239698128295473No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT870.2174076017692481No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.20241397406102404No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT760.18991928430417074No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.17242671864457607No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.1549341529849814No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG550.13744158732538672No Hit
TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.10995326986030936No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCA451.4895853E-552.7777751
GGTATCA3900.051.1538431
ACCCATG502.7779704E-547.5000043
CGCAGAA459.513908E-442.222229
GTATCAA5700.035.02
TACCCAT702.0182398E-433.9285742
CAACGCA6850.029.817526
TATCAAC6700.029.7761213
TCAACGC6900.029.6014485
ATCAACG7100.028.7676054
AACGCAG6850.028.4306587
GATTTTT350.00834579927.14285972-73
GCCGTAT350.00834579927.14285994-95
GCAGAGA906.501068E-926.38888710-11
ACGCAGA7700.025.2922088
GAGTACT3500.025.10714312-13
CGCAGAG7350.023.9115669
CAGAGAA1153.4997356E-922.71739210-11
GTACATG3400.022.352941
GCAGAGT6600.022.3106069
Produced by FastQC (version 0.11.5)