FastQCFastQC Report
Fri 10 Feb 2017
SRR2031774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40017
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT12813.201139515705825No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7451.8617087737711473No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7051.7617512557163206No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6331.5818277232176325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6121.5293500262388486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.8771272209311043No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.7921633305845015No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT3070.7671739510707949RNA PCR Primer, Index 33 (95% over 22bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.6122397980858135No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT2020.5047854661768748RNA PCR Primer, Index 33 (95% over 24bp)
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.3173651198240748No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.24489591923432544No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.23490016742884276No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT920.22990229152610142RNA PCR Primer, Index 33 (96% over 27bp)
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT850.21240972586650675No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG850.21240972586650675No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG840.2099107879151361RNA PCR Primer, Index 33 (96% over 29bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT740.1849214084014294No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.18242247045005872No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.13994052527675738No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.13494264937401604No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.13494264937401604No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG510.12744583551990404No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC490.12244795961716272No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG410.10245645600619738RNA PCR Primer, Index 15 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA856.9267116E-944.7052969
GGTATCA4650.040.9108121
GTATCAA6200.030.6447582
CTTGAAA2900.027.84446356-57
AACGCAG6850.027.736867
TGAAAAA2950.027.37252258-59
TATCAAC7000.027.14253
GCTTGAA2850.026.66631556-57
CAACGCA7150.026.5730766
TGCTTGA2950.026.56744854-55
TCAACGC7200.026.3885425
ACGCAGA7350.025.858
CTGCTTG3050.025.69638454-55
ATCAACG7450.025.5030214
CAGAAAA1051.2205419E-924.88062510-11
TTGAAAA3150.024.88062558-59
TCTGCTT3300.023.7496952-53
GCAGAAA801.8240044E-623.7496859
CGCAGAG6550.023.2058019
GAGTACT3550.022.7746471
Produced by FastQC (version 0.11.5)