##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031774_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40017 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73483769398006 34.0 31.0 34.0 31.0 34.0 2 32.8477647000025 34.0 33.0 34.0 31.0 34.0 3 32.84106754629283 34.0 33.0 34.0 31.0 34.0 4 36.27041007571782 37.0 37.0 37.0 35.0 37.0 5 36.29794837194193 37.0 37.0 37.0 35.0 37.0 6 36.43416547967114 37.0 37.0 37.0 35.0 37.0 7 36.379938526126395 37.0 37.0 37.0 35.0 37.0 8 36.42756828347952 37.0 37.0 37.0 35.0 37.0 9 38.190793912587154 39.0 39.0 39.0 37.0 39.0 10-11 38.1988030087213 39.0 39.0 39.0 37.0 39.0 12-13 38.21310942849289 39.0 39.0 39.0 37.0 39.0 14-15 39.6465877002274 41.0 40.0 41.0 37.0 41.0 16-17 39.554926656171126 41.0 39.0 41.0 37.0 41.0 18-19 39.56759627158458 41.0 39.5 41.0 36.5 41.0 20-21 39.5798410675463 41.0 40.0 41.0 36.0 41.0 22-23 39.43206637179199 41.0 39.0 41.0 35.0 41.0 24-25 39.29682384986381 41.0 39.0 41.0 35.0 41.0 26-27 39.07927880650723 41.0 38.5 41.0 35.0 41.0 28-29 38.91855961216483 41.0 38.0 41.0 35.0 41.0 30-31 38.62362246045431 40.0 37.5 41.0 35.0 41.0 32-33 37.954706749631406 40.0 35.0 41.0 34.5 41.0 34-35 37.88599845065848 40.0 35.0 41.0 35.0 41.0 36-37 37.74034785216283 40.0 35.0 41.0 34.5 41.0 38-39 37.34242946747632 40.0 35.0 41.0 33.0 41.0 40-41 37.24619536696904 40.0 35.0 41.0 33.0 41.0 42-43 36.97414848689307 40.0 35.0 41.0 33.0 41.0 44-45 36.566146887572785 40.0 35.0 41.0 31.5 41.0 46-47 36.16687907639253 40.0 35.0 41.0 29.0 41.0 48-49 35.7780318364695 40.0 35.0 41.0 25.0 41.0 50-51 35.540595247020015 39.5 35.0 41.0 23.0 41.0 52-53 35.51695529400005 39.0 35.0 41.0 22.5 41.0 54-55 35.38968438413674 39.0 35.0 41.0 21.0 41.0 56-57 35.12321013569233 39.0 35.0 41.0 20.0 41.0 58-59 34.82888522377989 38.5 35.0 41.0 18.0 41.0 60-61 34.47682235050104 38.0 35.0 41.0 16.5 41.0 62-63 34.085813529250075 37.0 35.0 41.0 14.5 41.0 64-65 33.69709123622461 37.0 35.0 40.0 11.0 41.0 66-67 33.19492965489667 36.0 34.0 40.0 10.0 41.0 68-69 32.48818002349002 35.5 33.5 39.0 9.0 41.0 70-71 31.85316240597746 35.0 33.0 39.0 8.0 41.0 72-73 31.30598245745558 35.0 32.5 37.5 8.0 40.5 74-75 30.93817627508309 35.0 32.0 37.0 7.0 39.5 76-77 29.725054351900443 34.5 29.5 36.0 7.0 39.0 78-79 30.343441537346628 35.0 31.0 36.0 7.0 39.0 80-81 30.21906939550691 35.0 31.0 36.0 7.0 37.5 82-83 29.976572456705902 35.0 31.0 35.5 7.0 37.0 84-85 29.724254691756002 35.0 30.0 35.0 7.0 37.0 86-87 29.555139065896995 35.0 30.0 35.0 7.0 36.0 88-89 29.381737761451383 35.0 30.0 35.0 7.0 36.0 90-91 29.17488817252668 35.0 29.0 35.0 7.0 36.0 92-93 29.034410375590376 35.0 29.0 35.0 7.0 36.0 94-95 28.897493565234775 35.0 29.0 35.0 7.0 35.0 96-97 28.79966014443861 35.0 29.0 35.0 7.0 35.0 98-99 28.68685808531374 35.0 27.0 35.0 7.0 35.0 100-101 27.772746582702354 34.0 25.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5319384768720781 1101 2 -0.03598964810766603 1101 3 -0.15334612627589905 1101 4 0.3485962212785836 1101 5 0.30919459841364016 1101 6 0.08885157284679224 1101 7 0.20256407532414755 1101 8 0.05473912007335713 1101 9 0.1426217310198723 1101 10-11 -0.01664777479301449 1101 12-13 0.3527702681136162 1101 14-15 0.7372481738339403 1101 16-17 0.37180099601878425 1101 18-19 0.19376811207538225 1101 20-21 -0.05058085334173512 1101 22-23 0.6142979918439835 1101 24-25 -0.38154104356304686 1101 26-27 0.31026849891578223 1101 28-29 0.6051061849024535 1101 30-31 0.8205850555217609 1101 32-33 1.1339739571625813 1101 34-35 0.9993508520200436 1101 36-37 1.351999125261834 1101 38-39 1.0507170329987972 1101 40-41 0.3475529897163838 1101 42-43 -0.4400483442700178 1101 44-45 0.10798490398288862 1101 46-47 0.8858893123353511 1101 48-49 1.2863303873273466 1101 50-51 0.5783147702435372 1101 52-53 0.13140914137843396 1101 54-55 -0.014384942529609646 1101 56-57 0.3427135920063762 1101 58-59 0.42538340597715063 1101 60-61 0.8781673907441956 1101 62-63 0.4808918935103108 1101 64-65 0.6372545124948275 1101 66-67 0.9356809860807331 1101 68-69 -0.9132498005574625 1101 70-71 -1.1894233939570285 1101 72-73 -1.5070348980093655 1101 74-75 -1.1758216628555331 1101 76-77 -1.3267728942404027 1101 78-79 -1.9866850671729068 1101 80-81 -1.7031004268141494 1101 82-83 -1.4714648477229915 1101 84-85 -1.517542367701708 1101 86-87 -2.661925597162206 1101 88-89 -1.8735676361750464 1101 90-91 -2.2369305048187655 1101 92-93 -1.5405128608771008 1101 94-95 -0.7690237031763978 1101 96-97 -1.4081831451912556 1101 98-99 -1.6247400166988974 1101 100-101 -1.9599127906896427 1102 1 0.41555916652725244 1102 2 0.3896569036164692 1102 3 0.31324870131030735 1102 4 0.19988932472686116 1102 5 -0.05826229813808226 1102 6 -0.053389806463549405 1102 7 -0.07114282122757487 1102 8 -0.04978674199561084 1102 9 -0.1677230965663341 1102 10-11 0.06417119072422395 1102 12-13 0.22453750949293294 1102 14-15 0.33746369107532104 1102 16-17 -0.17184124536053247 1102 18-19 -0.2507361982694434 1102 20-21 -0.28657223265208387 1102 22-23 -0.3264347667766998 1102 24-25 -0.698351388390634 1102 26-27 -1.2080504666014562 1102 28-29 -1.1292688150975465 1102 30-31 -1.6621735651678975 1102 32-33 -1.3288493186994899 1102 34-35 -1.461317251428234 1102 36-37 -1.1830224264623013 1102 38-39 -1.8118907256218932 1102 40-41 -1.2014771826974098 1102 42-43 -2.407720758063121 1102 44-45 -2.7131357856722857 1102 46-47 -2.7057055152508553 1102 48-49 -1.4475058195692085 1102 50-51 -1.4087541952737013 1102 52-53 -1.801241720690534 1102 54-55 -2.080656494253752 1102 56-57 -2.259657097648798 1102 58-59 -1.4140562491952622 1102 60-61 -2.9849791609799396 1102 62-63 -2.4954012099379597 1102 64-65 -1.6914092806086174 1102 66-67 -2.1812371173675373 1102 68-69 -2.419176524695395 1102 70-71 -1.498690635336338 1102 72-73 -1.4774012773197107 1102 74-75 -1.486705283545188 1102 76-77 -1.4038203080335059 1102 78-79 -0.89185748096601 1102 80-81 -0.9789624957796654 1102 82-83 -1.3362277787574754 1102 84-85 -1.2001932297706759 1102 86-87 -0.8343393902656544 1102 88-89 -1.2447960844509076 1102 90-91 -0.8829434358532495 1102 92-93 0.2057155873987604 1102 94-95 -0.8843254273143266 1102 96-97 -2.181890041742978 1102 98-99 -1.030451223595449 1102 100-101 -1.5520464113792976 1103 1 0.8905591665272539 1103 2 0.6709069036164692 1103 3 0.7236653679769773 1103 4 0.5623893247268583 1103 5 0.5896543685285849 1103 6 0.5049435268697806 1103 7 0.6163571787724251 1103 8 0.4731299246710563 1103 9 0.7656102367669959 1103 10-11 0.7454211907242225 1103 12-13 0.6182875094929301 1103 14-15 0.6062136910753182 1103 16-17 0.756283754639469 1103 18-19 0.4357221350638909 1103 20-21 0.5988444340145875 1103 22-23 0.37981523322329735 1103 24-25 0.6224819449426988 1103 26-27 0.7648662000652067 1103 28-29 0.7384395182357935 1103 30-31 0.3378264348321025 1103 32-33 0.013859014633844424 1103 34-35 0.002224415238437416 1103 36-37 0.4451025735376959 1103 38-39 0.4829009410447753 1103 40-41 0.6578978173025902 1103 42-43 1.1714459086035447 1103 44-45 1.3993642143277114 1103 46-47 1.5922111514158104 1103 48-49 1.3920775137641286 1103 50-51 1.3662458047262973 1103 52-53 2.4164666126428003 1103 54-55 3.1787185057462466 1103 56-57 2.500759569017873 1103 58-59 2.2713604174714064 1103 60-61 2.704604172353392 1103 62-63 2.150432123395369 1103 64-65 1.4148407193913854 1103 66-67 1.9718878826324655 1103 68-69 2.1068651419712765 1103 70-71 2.461726031330329 1103 72-73 2.1590570560136264 1103 74-75 2.408086383121475 1103 76-77 2.28472135863316 1103 78-79 2.1414758523673214 1103 80-81 2.2877041708870003 1103 82-83 2.471063887909196 1103 84-85 1.7841817702293241 1103 86-87 1.8489939430676792 1103 88-89 1.6499955822157588 1103 90-91 1.822264897480082 1103 92-93 1.969257254065429 1103 94-95 1.984424572685672 1103 96-97 2.6722766249236862 1103 98-99 2.831007109737886 1103 100-101 2.1906619219540353 1104 1 1.0258532841743104 1104 2 0.9062010212635272 1104 3 0.8922928189573653 1104 4 0.49768344237391915 1104 5 0.6641641724501497 1104 6 0.5127866641246825 1104 7 0.49871011994889614 1104 8 0.4299926697690921 1104 9 0.5969827857866079 1104 10-11 0.8307153083712819 1104 12-13 0.700640450669404 1104 14-15 0.762096044016495 1104 16-17 0.8180484605218155 1104 18-19 0.8347417429070276 1104 20-21 0.6968836497008581 1104 22-23 0.7415799391056552 1104 24-25 0.812678023374076 1104 26-27 0.8746701216338337 1104 28-29 0.9286355966671636 1104 30-31 0.8966499642438706 1104 32-33 0.8687609754181551 1104 34-35 1.056145983865882 1104 36-37 1.1598084558906407 1104 38-39 1.9427048626133967 1104 40-41 1.4814272290672932 1104 42-43 1.919485124289821 1104 44-45 1.9228936260924243 1104 46-47 0.5461327200432606 1104 48-49 0.03227359219550152 1104 50-51 0.8338928635498277 1104 52-53 0.3301921028388719 1104 54-55 1.014012623393306 1104 56-57 1.6135046670570858 1104 58-59 1.6135172802165059 1104 60-61 1.4212708390200604 1104 62-63 1.5818046724149823 1104 64-65 2.4060171899796146 1104 66-67 2.0954172943971656 1104 68-69 2.190198475304605 1104 70-71 2.15388289407543 1104 72-73 2.3433707815038183 1104 74-75 2.25318442233716 1104 76-77 2.31217233902532 1104 78-79 2.255201342563396 1104 80-81 1.8004492689262186 1104 82-83 1.657338397713115 1104 84-85 0.6077111819940271 1104 86-87 0.9009547273814036 1104 88-89 1.5539171508432084 1104 90-91 1.2595197994408664 1104 92-93 1.0467082344575864 1104 94-95 1.0991304550386154 1104 96-97 1.917374664139377 1104 98-99 1.782967894051609 1104 100-101 1.3367403533265865 1105 1 0.11913059509868162 1105 2 0.05662118933075533 1105 3 0.27128441559602123 1105 4 -0.3233249609874278 1105 5 -0.3055837267095143 1105 6 0.17161019353645202 1105 7 -0.1550713926561471 1105 8 -0.10782245628132614 1105 9 0.4037054748622353 1105 10-11 0.259706905009935 1105 12-13 0.07543036663579272 1105 14-15 0.09192797678960574 1105 16-17 0.1634266117823202 1105 18-19 0.5666745160162705 1105 20-21 0.4036063387764912 1105 22-23 0.23695809036615856 1105 24-25 0.4677200401807937 1105 26-27 0.5624852476842577 1105 28-29 0.07653475633102147 1105 30-31 -0.4478878508821822 1105 32-33 -0.3123314615566315 1105 34-35 -0.5572993942853728 1105 36-37 -0.46918314074801515 1105 38-39 -1.5551942970504626 1105 40-41 -1.027816468411693 1105 42-43 -0.595220758063121 1105 44-45 -0.43635007138657045 1105 46-47 -1.795884086679429 1105 48-49 -1.3126843909977808 1105 50-51 -0.28375419527370127 1105 52-53 -0.902581006404823 1105 54-55 -0.4641386371108922 1105 56-57 -0.644478526220226 1105 58-59 -0.6238776777666928 1105 60-61 -0.3144434466942201 1105 62-63 -0.14718692422367496 1105 64-65 -0.8208735663229021 1105 66-67 -0.820969260224679 1105 68-69 -1.2240872389811095 1105 70-71 -1.5835120639077687 1105 72-73 -1.2814191344625705 1105 74-75 -0.7157231406880449 1105 76-77 -0.6819453080335087 1105 78-79 0.27242823331970456 1105 80-81 0.12103750422033599 1105 82-83 0.5043972212425238 1105 84-85 0.11989605594360953 1105 86-87 0.43708918116291784 1105 88-89 1.0190432012633792 1105 90-91 0.8817887070038921 1105 92-93 0.7097334445416159 1105 94-95 1.0915674298285296 1105 96-97 1.334181386828451 1105 98-99 0.6905309192616969 1105 100-101 -0.7426714113792947 1106 1 0.5800328507377799 1106 2 0.3551174299322568 1106 3 0.31489343815241 1106 4 0.1834419563058063 1106 5 0.27561928080928766 1106 6 0.09792598301013555 1106 7 0.30056770508821273 1106 8 0.13277904747807412 1106 9 0.3322769034336659 1106 10-11 0.029631717040018657 1106 12-13 0.05512961475609046 1106 14-15 -0.167470519450994 1106 16-17 -0.22529519272895726 1106 18-19 -0.13971646142733363 1106 20-21 -0.09413802212576883 1106 22-23 0.2640257595390878 1106 24-25 -0.010851388390634042 1106 26-27 -0.03864257186461373 1106 28-29 -0.07910434141334122 1106 30-31 -0.5305946177994727 1106 32-33 -0.10105326606791465 1106 34-35 -0.1512843566913915 1106 36-37 0.11878678406401377 1106 38-39 0.09518164279916164 1106 40-41 -0.9078916563816151 1106 42-43 -1.6346944422736485 1106 44-45 -1.7164252593564981 1106 46-47 -1.60866604156665 1106 48-49 -1.7517821353586882 1106 50-51 -1.6127015636947561 1106 52-53 -1.2510772470063216 1106 54-55 -1.944965704780067 1106 56-57 -1.8369597292277433 1106 58-59 -1.7874115123531524 1106 60-61 -1.9471502136115149 1106 62-63 -0.8934275257274393 1106 64-65 -0.9693698069244121 1106 66-67 -1.0833752752622772 1106 68-69 -1.7966436299585524 1106 70-71 -1.5365195827047593 1106 72-73 -1.0242762773197107 1106 74-75 -1.5664750203872941 1106 76-77 -2.337208465928242 1106 78-79 -1.5497522178081127 1106 80-81 -1.939488811569138 1106 82-83 -1.7613922524416878 1106 84-85 -2.2289761245075184 1106 86-87 -2.202760442897233 1106 88-89 -1.7850921370824864 1106 90-91 -1.776857909537462 1106 92-93 -1.3263567810222945 1106 94-95 -1.507680690472224 1106 96-97 -2.665442673321923 1106 98-99 -2.676010434121764 1106 100-101 -1.1663556219056126 1107 1 0.45272132868941384 1107 2 0.36009609280565513 1107 3 0.48132302563463014 1107 4 0.275902838240377 1107 5 0.3446093234835388 1107 6 0.19323181515807164 1107 7 0.2650058274210707 1107 8 0.20105785259898568 1107 9 0.48092555208231147 1107 10-11 0.3859617312647643 1107 12-13 0.3858550770604978 1107 14-15 0.1035109883726193 1107 16-17 0.3657432140989272 1107 18-19 0.3442806936224443 1107 20-21 0.2970426322127864 1107 22-23 0.30954496295302647 1107 24-25 0.5229323953931484 1107 26-27 0.3671184523174631 1107 28-29 0.41591699571326046 1107 30-31 0.1621507591564253 1107 32-33 0.23322838400321189 1107 34-35 -0.15768549467147608 1107 36-37 0.25321068164581106 1107 38-39 0.3810991392429699 1107 40-41 0.03627619568096918 1107 42-43 0.45883329599093514 1107 44-45 0.16017502513852122 1107 46-47 -0.3974284882238237 1107 48-49 0.2290144507010652 1107 50-51 1.0946241831046777 1107 52-53 0.7642143603905467 1107 54-55 0.7652049922327322 1107 56-57 0.9354442537025562 1107 58-59 0.8587478048587869 1107 60-61 0.8874870552362779 1107 62-63 1.458990681953928 1107 64-65 1.005381259931923 1107 66-67 1.0759419366865117 1107 68-69 0.8388471239532507 1107 70-71 1.0612755808798795 1107 72-73 0.6108588578154261 1107 74-75 0.3954737705088682 1107 76-77 0.5180546919664941 1107 78-79 0.06084522173669171 1107 80-81 -0.23571925253642334 1107 82-83 0.48007289691820176 1107 84-85 0.5747223107698645 1107 86-87 0.7872822313559666 1107 88-89 0.7387343209544994 1107 90-91 0.33622886144404873 1107 92-93 -0.3149769801688045 1107 94-95 -0.0709808327197301 1107 96-97 0.7758802285272921 1107 98-99 0.11524134397211583 1107 100-101 -0.09132006002794668 1108 1 -0.5844408334727476 1108 2 -0.25409309638352795 1108 3 -0.1680012986896955 1108 4 -0.36261067527313884 1108 5 -0.7020122981380794 1108 6 -0.7783898064635508 1108 7 -1.1586428212275734 1108 8 -0.5435367419956094 1108 9 -1.2177230965663313 1108 10-11 -0.842078809275776 1108 12-13 -0.5442124905070642 1108 14-15 -0.28128630892467754 1108 16-17 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-4.206353248833324 2316 70-71 -2.3618371870604733 2316 72-73 -1.5846211049059171 2316 74-75 -0.8223733869934655 2316 76-77 -0.5767728942404027 2316 78-79 -0.1504781706211844 2316 80-81 -0.694479737158975 2316 82-83 0.30439722124252455 2316 84-85 0.1634921150569113 2316 86-87 -0.661925597162206 2316 88-89 -0.4166710844509076 2316 90-91 -0.39210291861186874 2316 92-93 0.22672851843324437 2316 94-95 -0.3638512893832946 2316 96-97 -0.14094176588091045 2316 98-99 0.14250136261144775 2316 100-101 0.6435354851724249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 6.0 15 22.0 16 71.0 17 214.0 18 402.0 19 452.0 20 611.0 21 690.0 22 784.0 23 681.0 24 592.0 25 511.0 26 578.0 27 718.0 28 920.0 29 1037.0 30 646.0 31 578.0 32 672.0 33 796.0 34 1218.0 35 3131.0 36 3417.0 37 7037.0 38 10983.0 39 3245.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10491278123983 20.524563906199162 14.613209199889882 20.75731411267112 2 16.055676337556537 23.21013569233076 41.652297773446286 19.081890196666418 3 16.42801809231077 30.502036634430368 35.85975960216908 17.210185671089786 4 9.815828272983982 24.457105730064725 37.41909688382437 28.30796911312692 5 9.2860534272934 38.4136741884699 38.318714546317814 13.981557837918885 6 22.39298298223255 41.372416722892766 18.30472049379014 17.92987980108454 7 19.55418946947547 37.54404378139291 23.85736062173576 19.044406127395856 8 22.527925631606568 35.48241997151211 23.657445585626107 18.332208811255217 9 24.282180073468776 14.998625584126746 22.31801484369143 38.40117949871305 10-11 21.21973161406402 28.063073193892595 30.022240547767197 20.694954644276184 12-13 24.167228927705725 27.198440662718344 29.983507009520956 18.65082340005498 14-15 21.335932228802758 27.104730489541947 29.24507084489092 22.314266436764374 16-17 18.502136591948425 32.14883674438364 29.63990304120749 19.709123622460453 18-19 18.284728990179175 27.49581427893145 34.30666966539221 19.912787065497163 20-21 18.881975160556763 26.831096783866858 34.32291276207612 19.96401529350026 22-23 17.87615263513007 29.371267211435143 34.51533098433166 18.23724916910313 24-25 18.755778794012546 30.775670340105453 33.72441712272284 16.744133743159157 26-27 18.542119599170352 30.424569557937875 34.25419196841342 16.779118874478346 28-29 17.804932903516004 28.756528475397957 35.306244845940476 18.132293775145563 30-31 19.83157158207762 28.96518979433741 34.85143813879101 16.351800484793962 32-33 17.481320438813505 29.82732338756029 36.15963215633356 16.53172401729265 34-35 16.83159657145713 28.350451058300223 35.71732013894095 19.1006322313017 36-37 16.388035085088838 31.450383586975533 34.18921958167779 17.97236174625784 38-39 16.819101881700277 27.60201914186471 36.29832321263463 19.280555763800386 40-41 15.965714571307194 27.870654971637055 37.17295149561436 18.990678961441386 42-43 18.14353899592673 27.998100807156955 36.418272234300424 17.440087962615888 44-45 16.724142239548193 29.127620761176498 36.18961941175001 17.958617587525303 46-47 18.662068620836145 27.46207861658795 33.75815278506635 20.11769997750956 48-49 18.18125023427836 27.674834130920996 33.68235602813839 20.461559606662252 50-51 16.77162206062423 27.900642227053503 34.706499737611516 20.621235974710746 52-53 16.50298623085189 28.452907514306418 36.38953444785966 18.65457180698203 54-55 16.17562535922233 28.680310867881147 34.23544993377814 20.908613839118377 56-57 18.785766049428993 28.70779918534623 32.00139940525277 20.505035359972013 58-59 16.286828098058326 29.353774645775545 35.677337131719014 18.682060124447112 60-61 17.893645200789663 31.20798660569258 31.61031561586326 19.288052577654497 62-63 16.043181647799685 33.43454032036385 31.345428193017966 19.1768498388185 64-65 16.134392883024713 33.39455731314192 31.148012094859684 19.323037708973686 66-67 16.101906689656893 33.4470350101207 30.644476097658497 19.80658220256391 68-69 16.431766499237824 33.04845440687708 30.23340080465802 20.286378289227077 70-71 16.7403853362321 32.61238973436289 30.11844965889497 20.52877527051003 72-73 17.401354424369643 31.404153234875174 30.152185321238473 21.042307019516706 74-75 17.071494614788715 31.008071569582928 30.35959717120224 21.560836644426118 76-77 17.53379813579229 31.46037933878102 30.094709748356944 20.911112777069746 78-79 17.620011495114575 30.649473973561236 29.989754354399377 21.740760176924805 80-81 17.268910712946997 31.28545368218507 29.791088787265412 21.65454681760252 82-83 17.537546542719344 31.65154809206087 29.36876827348377 21.442137091736015 84-85 17.108978684059277 30.484544068770774 29.926031436639427 22.480445810530526 86-87 17.6774870679961 31.16550466051928 29.370017742459453 21.786990529025164 88-89 17.663742909263565 30.38583601969163 29.76734887672739 22.183072194317415 90-91 17.926131394157483 30.063473023964814 29.8348202014144 22.175575380463304 92-93 17.682484943898842 30.17217682484944 29.85356223604968 22.29177599520204 94-95 17.74995626858585 29.69737861408901 29.98725541644801 22.565409700877126 96-97 18.046080415823276 29.541194992128343 29.94852187820176 22.464202713846614 98-99 17.190194167478822 29.573681185496163 30.1159507209436 23.120173926081417 100-101 17.28390434065522 29.763600469800334 30.053477272159334 22.899017917385112 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1155.0 1 679.0 2 215.5 3 228.0 4 156.0 5 95.0 6 143.0 7 289.5 8 438.0 9 438.5 10 285.0 11 152.0 12 138.5 13 141.0 14 130.5 15 133.5 16 129.5 17 121.0 18 138.0 19 144.0 20 143.0 21 161.5 22 179.5 23 216.0 24 254.5 25 289.5 26 341.0 27 395.5 28 454.0 29 515.5 30 595.5 31 699.5 32 811.5 33 913.0 34 992.0 35 1069.5 36 1295.5 37 1462.5 38 1385.0 39 1344.5 40 1311.5 41 1217.0 42 1172.0 43 1260.0 44 1392.5 45 1387.5 46 1184.0 47 1046.5 48 1099.0 49 1214.5 50 1444.0 51 1449.5 52 1096.5 53 763.5 54 599.5 55 535.0 56 524.5 57 487.0 58 393.0 59 359.0 60 331.0 61 284.5 62 268.0 63 249.0 64 193.5 65 116.5 66 89.0 67 67.0 68 45.0 69 30.0 70 20.0 71 20.0 72 15.5 73 14.5 74 15.0 75 10.5 76 5.0 77 2.5 78 3.0 79 4.0 80 3.5 81 1.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14993627708224005 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0012494689756853338 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 40017.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.81340430317115 #Duplication Level Percentage of deduplicated Percentage of total 1 75.08606275204092 38.15378464152735 2 12.747123045146061 12.954494339905539 3 4.8145962427461395 7.33938076317565 4 2.390085570964886 4.857935377464577 5 1.4704435920133767 3.735912237299148 6 0.9343955935870955 2.8487892645625608 7 0.595062456968624 2.1166004448109557 8 0.3983475951608144 1.6193117924881923 9 0.28031867807612865 1.2819551690531525 >10 1.170453427756467 9.036159632156334 >50 0.05901445854234287 2.2490441562336008 >100 0.029507229271171435 3.870854886673164 >500 0.019671486180780958 6.734637778943949 >1k 0.0049178715451952395 3.201139515705825 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 1281 3.201139515705825 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 745 1.8617087737711473 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 705 1.7617512557163206 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 633 1.5818277232176325 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 612 1.5293500262388486 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 351 0.8771272209311043 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 317 0.7921633305845015 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 307 0.7671739510707949 RNA PCR Primer, Index 33 (95% over 22bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 245 0.6122397980858135 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 202 0.5047854661768748 RNA PCR Primer, Index 33 (95% over 24bp) GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 127 0.3173651198240748 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 98 0.24489591923432544 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 94 0.23490016742884276 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 92 0.22990229152610142 RNA PCR Primer, Index 33 (96% over 27bp) GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 85 0.21240972586650675 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 85 0.21240972586650675 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 84 0.2099107879151361 RNA PCR Primer, Index 33 (96% over 29bp) GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 74 0.1849214084014294 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73 0.18242247045005872 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.13994052527675738 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 54 0.13494264937401604 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 54 0.13494264937401604 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 51 0.12744583551990404 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC 49 0.12244795961716272 No Hit CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG 41 0.10245645600619738 RNA PCR Primer, Index 15 (95% over 24bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0024989379513706673 0.0 10-11 0.0 0.0 0.0 0.0024989379513706673 0.0 12-13 0.0 0.0 0.0 0.0024989379513706673 0.0 14-15 0.0 0.0 0.0 0.0024989379513706673 0.0 16-17 0.0 0.0 0.0 0.0024989379513706673 0.0 18-19 0.0 0.0 0.0 0.0024989379513706673 0.0 20-21 0.0 0.0 0.0 0.011245220781168002 0.0 22-23 0.0 0.0 0.0 0.024989379513706673 0.0 24-25 0.0 0.0 0.0 0.03873353824624534 0.0 26-27 0.0 0.0 0.0 0.07621760751680536 0.0 28-29 0.0 0.0 0.0 0.2261538845990454 0.0 30-31 0.0 0.0 0.0 0.4373141414898668 0.0 32-33 0.0 0.0 0.0 0.6422270535022616 0.0 34-35 0.0 0.0 0.0 1.033310842891771 0.0 36-37 0.0 0.0 0.0 1.4881175500412325 0.0 38-39 0.0 0.0 0.0 1.9604168228502885 0.0 40-41 0.0 0.0 0.0 2.706349801334433 0.0 42-43 0.0 0.0 0.0 3.179898543119174 0.0 44-45 0.0 0.0 0.0 3.5197541045055853 0.0 46-47 0.0 0.0 0.0 3.804633030961841 0.0 48-49 0.0 0.0 0.0 4.079516205612615 0.0 50-51 0.0 0.0 0.0 4.363145663093185 0.0 52-53 0.0 0.0 0.0 4.580553264862433 0.0 54-55 0.0 0.0 0.0 4.949146612689606 0.0 56-57 0.0 0.0 0.0 5.533898093310343 0.0 58-59 0.0 0.0 0.0 5.89874303421046 0.0 60-61 0.0 0.0 0.0 6.187370367593773 0.0 62-63 0.0 0.0 0.0 6.434765224779468 0.0 64-65 0.0 0.0 0.0 6.692155833770648 0.0 66-67 0.0 0.0 0.0 7.000774670764924 0.0 68-69 0.0 0.0 0.0 7.418097308643826 0.0 70-71 0.0 0.0 0.0 7.867906139890547 0.0 72-73 0.0 0.0 0.0 8.24149736362046 0.0 74-75 0.0 0.0 0.0 8.647574780718195 0.0 76-77 0.0 0.0 0.0 9.059899542694355 0.0 78-79 0.0 0.0 0.0 9.419746607691732 0.0 80-81 0.0 0.0 0.0 9.849563935327485 0.0 82-83 0.0 0.0 0.0 10.296873828622836 0.0 84-85 0.0 0.0 0.0 10.726691156258589 0.0 86-87 0.0 0.0 0.0 11.218981932678611 0.0 88-89 0.0 0.0 0.0 11.707524302171578 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 85 6.9267116E-9 44.705296 9 GGTATCA 465 0.0 40.910812 1 GTATCAA 620 0.0 30.644758 2 CTTGAAA 290 0.0 27.844463 56-57 AACGCAG 685 0.0 27.73686 7 TGAAAAA 295 0.0 27.372522 58-59 TATCAAC 700 0.0 27.1425 3 GCTTGAA 285 0.0 26.666315 56-57 CAACGCA 715 0.0 26.573076 6 TGCTTGA 295 0.0 26.567448 54-55 TCAACGC 720 0.0 26.388542 5 ACGCAGA 735 0.0 25.85 8 CTGCTTG 305 0.0 25.696384 54-55 ATCAACG 745 0.0 25.503021 4 CAGAAAA 105 1.2205419E-9 24.880625 10-11 TTGAAAA 315 0.0 24.880625 58-59 TCTGCTT 330 0.0 23.74969 52-53 GCAGAAA 80 1.8240044E-6 23.749685 9 CGCAGAG 655 0.0 23.205801 9 GAGTACT 355 0.0 22.774647 1 >>END_MODULE