FastQCFastQC Report
Fri 10 Feb 2017
SRR2031773_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031773_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390887
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131540.5501723837220246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105770.44238811788261007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62700.2622457690388546No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50400.067.008931
GTATCAA96300.045.8722761
TCAACGC122450.035.6880344
ATCAACG123350.035.4276433
CAACGCA124100.035.2518125
TATCAAC127700.034.4068152
AACGCAG127600.034.3965536
ACGCAGA142100.030.8532717
CGCAGAG143950.030.3577658
GTACATG103850.028.2667311
GCAGAGT155550.027.2999049
TACATGG105400.027.0398482
ACATGGG104700.026.1318053
CAGAGTA153100.024.15333210-11
GAGTACT100600.024.033312-13
CATGGGG54450.023.5537174
AGAGTAC147700.023.25152410-11
GTACTTT108500.022.13018414-15
AGTACTT107600.021.05715612-13
GTGGTAT19200.021.0286461
Produced by FastQC (version 0.11.5)