FastQCFastQC Report
Fri 10 Feb 2017
SRR2031773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390887
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161040.6735575541629529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133380.557868272319018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80080.33493845589523885No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT25740.1076587893948982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55900.066.639881
GTATCAA109200.045.397231
TCAACGC139900.035.310184
ATCAACG139900.035.2083243
TATCAAC142500.034.699262
CAACGCA142100.034.6966555
AACGCAG146300.033.7979776
ACGCAGA161300.030.4782717
CGCAGAG162050.030.278598
GTACATG96900.027.4946691
GCAGAGT177550.027.2340019
TACATGG97650.026.9477082
ACATGGG96950.026.6033543
CATGGGG45250.025.5077424
CAGAGTA174700.024.06216610-11
GAGTACT128650.023.88794912-13
AGAGTAC168550.022.99565310-11
GTGGTAT21300.022.1126021
TGGTATC21000.021.9400082
GTACTTT139750.021.85463114-15
Produced by FastQC (version 0.11.5)