FastQCFastQC Report
Fri 10 Feb 2017
SRR2031772_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031772_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492639
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39450.15826599840570577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32390.12994260300027402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30250.055.5955431
GTATCAA51450.039.427991
TCAACGC60700.032.6369444
ATCAACG63000.031.5208453
CAACGCA64400.030.7618395
TATCAAC65550.030.5845342
AACGCAG67700.029.3254746
GTGGTAT14650.026.2669411
ACGCAGA75000.026.0916847
CGCAGAG79000.024.411818
GTACATG64600.022.577091
TGGTATC16700.021.3357012
GCAGAGT88450.020.6758779
TACATGG68850.020.562432
CAGAGTA79950.020.2619210-11
ACATGGG72850.019.629043
GAGTACT43800.019.09034512-13
AGAGTAC74350.018.20994210-11
GTACTTT48200.016.80571214-15
CATGGGG48350.016.3107434
Produced by FastQC (version 0.11.5)