##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031772_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2492639
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98579457354234	33.0	31.0	34.0	30.0	34.0
2	32.291744211656805	34.0	31.0	34.0	31.0	34.0
3	32.40303870716939	34.0	31.0	34.0	31.0	34.0
4	35.82306864331337	37.0	37.0	37.0	35.0	37.0
5	35.81711350901595	37.0	37.0	37.0	35.0	37.0
6	35.845692858051244	37.0	37.0	37.0	35.0	37.0
7	35.823450969033225	37.0	37.0	37.0	35.0	37.0
8	35.80819845954428	37.0	37.0	37.0	35.0	37.0
9	37.58977092150127	39.0	38.0	39.0	35.0	39.0
10-11	37.518585322623935	39.0	38.0	39.0	35.0	39.0
12-13	37.46631040435459	39.0	38.0	39.0	35.0	39.0
14-15	38.88357158818425	41.0	39.0	41.0	36.0	41.0
16-17	38.80871919279126	41.0	39.0	41.0	36.0	41.0
18-19	38.80059567390224	41.0	39.0	41.0	35.5	41.0
20-21	38.79022794716764	41.0	39.0	41.0	35.0	41.0
22-23	38.688092820500685	41.0	39.0	41.0	35.0	41.0
24-25	38.614472051508464	41.0	39.0	41.0	35.0	41.0
26-27	38.49614765716174	40.0	38.5	41.0	34.5	41.0
28-29	38.383018158666374	40.0	38.0	41.0	34.0	41.0
30-31	38.23490304853611	40.0	38.0	41.0	34.0	41.0
32-33	38.04893227619402	40.0	38.0	41.0	34.0	41.0
34-35	37.89487105834419	40.0	38.0	41.0	33.0	41.0
36-37	37.73253567805045	40.0	38.0	41.0	33.0	41.0
38-39	37.54892525552236	40.0	38.0	41.0	33.0	41.0
40-41	37.45339778443649	40.0	38.0	41.0	32.5	41.0
42-43	37.43427467836297	40.0	38.0	41.0	33.0	41.0
44-45	37.3005056087143	40.0	37.5	41.0	32.0	41.0
46-47	37.25337122623854	40.0	37.5	41.0	32.0	41.0
48-49	37.326812065445495	40.0	37.5	41.0	32.0	41.0
50-51	36.76739712409218	39.5	36.5	40.5	31.5	41.0
52-53	36.771354375824174	39.5	37.0	40.5	31.5	41.0
54-55	36.9727389325129	40.0	37.0	41.0	32.0	41.0
56-57	36.81771247260434	40.0	36.0	41.0	31.0	41.0
58-59	36.59692157588805	39.5	36.0	41.0	31.0	41.0
60-61	36.32316713330731	39.0	35.0	41.0	31.0	41.0
62-63	36.01482164083929	39.0	35.0	41.0	30.5	41.0
64-65	35.70882245684193	38.0	35.0	41.0	30.0	41.0
66-67	35.390486147412446	37.0	35.0	40.0	30.0	41.0
68-69	35.031598639032765	37.0	35.0	40.0	29.0	41.0
70-71	34.65527338696057	36.0	35.0	39.0	29.0	41.0
72-73	34.2842968837445	36.0	34.5	39.0	29.0	41.0
74-75	33.88256341973306	35.0	34.0	38.5	28.5	40.0
76-77	33.514239125681655	35.0	34.0	37.0	28.0	39.0
78-79	33.14764412335681	35.0	34.0	37.0	27.0	39.0
80-81	32.82561855126234	35.0	34.0	36.0	26.5	39.0
82-83	32.528052196888524	35.0	34.0	36.0	26.0	37.0
84-85	32.24154219684439	35.0	34.0	35.5	26.0	37.0
86-87	31.99602048270929	35.0	33.0	35.0	25.5	36.5
88-89	31.75311266493062	35.0	33.0	35.0	25.0	36.0
90-91	31.53663647242942	35.0	33.0	35.0	24.5	36.0
92-93	31.342860317920085	35.0	33.0	35.0	24.0	36.0
94-95	31.100594791303514	35.0	33.0	35.0	21.5	35.0
96-97	30.844405467458383	35.0	33.0	35.0	19.5	35.0
98-99	30.59461779262862	35.0	32.0	35.0	16.5	35.0
100-101	29.997508062739932	34.5	31.5	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.06077473991883764
1101	2	0.043810889033693456
1101	3	0.11597245894772357
1101	4	0.30103014145649354
1101	5	0.2410957489543577
1101	6	0.2846963987950488
1101	7	0.3485987939911581
1101	8	0.325552075945474
1101	9	0.3200305948010893
1101	10-11	0.1899640052155931
1101	12-13	0.237211261061816
1101	14-15	0.3336960719445017
1101	16-17	0.34977190272089587
1101	18-19	0.3432991231209854
1101	20-21	0.36887604634631543
1101	22-23	0.41334667096429456
1101	24-25	0.3201897752562033
1101	26-27	0.19394723639211264
1101	28-29	0.2076905309041024
1101	30-31	0.3708175443445114
1101	32-33	0.2851883885966089
1101	34-35	0.43274115401029434
1101	36-37	0.1483173839295162
1101	38-39	0.3498330447697313
1101	40-41	0.10076077497470948
1101	42-43	0.1651265583482342
1101	44-45	-0.0665594665305207
1101	46-47	0.09000624949874236
1101	48-49	0.29890697380938036
1101	50-51	0.3403591524583973
1101	52-53	0.25171637712500683
1101	54-55	0.3115797648417029
1101	56-57	0.23470264434714494
1101	58-59	0.07900969607187136
1101	60-61	-0.10337875749320347
1101	62-63	0.17886809411379545
1101	64-65	0.3482173340420829
1101	66-67	-0.01721158664094702
1101	68-69	-0.014445399160202044
1101	70-71	-0.14971836528656723
1101	72-73	0.3090161143476635
1101	74-75	0.37319455308715277
1101	76-77	0.01107863243629481
1101	78-79	-0.22345586800173578
1101	80-81	-0.26009935168750786
1101	82-83	-0.13818261365859286
1101	84-85	-0.21849487305350124
1101	86-87	-0.3353420293688387
1101	88-89	-0.5402989193230745
1101	90-91	-0.932285917046265
1101	92-93	-0.7175113046045567
1101	94-95	-0.6445174115558174
1101	96-97	-0.697046739683497
1101	98-99	-0.7735472873785838
1101	100-101	-0.8715658102323758
1102	1	0.0498876024930226
1102	2	0.14181393530241593
1102	3	0.21046707171392143
1102	4	0.30988361110834717
1102	5	0.21542350014091483
1102	6	0.24883241188033622
1102	7	0.2140485606984157
1102	8	0.10460917941813364
1102	9	0.33553923728247526
1102	10-11	0.2423855477206942
1102	12-13	0.34562708076128956
1102	14-15	0.4504770741588757
1102	16-17	0.28452629228159765
1102	18-19	0.2806878519267144
1102	20-21	0.21919895886856722
1102	22-23	0.2819471958227311
1102	24-25	0.14507353076701435
1102	26-27	0.21069769554276974
1102	28-29	0.16334883439766656
1102	30-31	0.16623929984728392
1102	32-33	0.2072895102504333
1102	34-35	0.2789648658766666
1102	36-37	0.16584146673336875
1102	38-39	-0.09807294689522905
1102	40-41	0.0818903860730984
1102	42-43	0.1777235627167073
1102	44-45	0.10711402008076476
1102	46-47	0.08240665298868777
1102	48-49	0.14377835134174433
1102	50-51	0.047149759662538315
1102	52-53	-0.06105464233012725
1102	54-55	0.1051327671043012
1102	56-57	0.05667878056920728
1102	58-59	-0.056370424412484965
1102	60-61	-0.14784101872480448
1102	62-63	-0.14396405165699377
1102	64-65	-0.003039955100256009
1102	66-67	-0.2631756824657927
1102	68-69	-0.1712800124890279
1102	70-71	-0.1522281209822438
1102	72-73	-0.1084513030353591
1102	74-75	-0.4758805145295639
1102	76-77	-0.09819520622399125
1102	78-79	-0.17337729998916274
1102	80-81	-0.10050983438848249
1102	82-83	-0.06837362837150351
1102	84-85	7.289838793411718E-5
1102	86-87	-0.095671412198012
1102	88-89	-0.14665710104626584
1102	90-91	-0.0525894186477629
1102	92-93	-0.0307826676691505
1102	94-95	-0.24487950761375288
1102	96-97	-0.567083994183573
1102	98-99	-0.31013868146850143
1102	100-101	-0.31113475115164135
1103	1	-0.06890741021599212
1103	2	0.10700703167478309
1103	3	0.15799638307708364
1103	4	0.05855479006898179
1103	5	0.08463494600527355
1103	6	0.03155503952190486
1103	7	0.030664926638038992
1103	8	0.12954030329564148
1103	9	0.12614049812145112
1103	10-11	0.07973305455276858
1103	12-13	0.1289128011079086
1103	14-15	0.23030804861134158
1103	16-17	0.2607462320717744
1103	18-19	0.17713821325008894
1103	20-21	-0.014451749950751491
1103	22-23	0.057684808394064646
1103	24-25	0.08795671646344516
1103	26-27	0.07777061082808956
1103	28-29	0.022733812772457895
1103	30-31	0.11802046092001461
1103	32-33	-0.1542548786695903
1103	34-35	-0.06726573380262124
1103	36-37	-0.06340790070361635
1103	38-39	-0.04525374052497
1103	40-41	0.02111446875954215
1103	42-43	0.08375749532727639
1103	44-45	-0.17538392579702844
1103	46-47	-0.07662716433781469
1103	48-49	0.1447198379020449
1103	50-51	0.06052315587566426
1103	52-53	0.18098370439415845
1103	54-55	0.06721152766951377
1103	56-57	0.02607974544577729
1103	58-59	-0.04506736569734926
1103	60-61	0.08511898525571837
1103	62-63	0.16148153237453755
1103	64-65	0.19742726746218153
1103	66-67	-0.023894601914214775
1103	68-69	0.011193566023969481
1103	70-71	0.10015713749508137
1103	72-73	0.04756036131340835
1103	74-75	-4.36465397662289E-4
1103	76-77	-0.012560800659969118
1103	78-79	-0.04931542687405255
1103	80-81	-0.01753618068538998
1103	82-83	-0.0015389668279794932
1103	84-85	-0.09418626597361168
1103	86-87	0.013321011706175057
1103	88-89	-0.036633340089000654
1103	90-91	-0.12644796753897936
1103	92-93	-0.7104934268269574
1103	94-95	-0.22022182244232624
1103	96-97	-0.05173122160172383
1103	98-99	-0.20114925628286784
1103	100-101	-0.09289190248452783
1104	1	0.14220778390414068
1104	2	0.2118655429765326
1104	3	0.2565625007759351
1104	4	0.14903489182502483
1104	5	0.18120660706246383
1104	6	0.27432702543738685
1104	7	0.338433234736506
1104	8	0.2660974727796912
1104	9	0.2597858811191003
1104	10-11	0.32961890647053593
1104	12-13	0.3437422898840907
1104	14-15	0.4597573004362161
1104	16-17	0.46309166168204285
1104	18-19	0.39023459941402905
1104	20-21	0.3268878044789716
1104	22-23	0.42647845332523815
1104	24-25	0.46984303761635005
1104	26-27	0.3762286220544908
1104	28-29	0.2713462632970689
1104	30-31	0.425201499674813
1104	32-33	0.45311323236669665
1104	34-35	0.4787088441810923
1104	36-37	0.5050327608433349
1104	38-39	0.4082459681720252
1104	40-41	0.49125844975137056
1104	42-43	0.4574277453006914
1104	44-45	0.4765868100130106
1104	46-47	0.46310528043326826
1104	48-49	0.6869262338009605
1104	50-51	0.5451916642488399
1104	52-53	0.6870005467027553
1104	54-55	0.6917252713473303
1104	56-57	0.6247002687217247
1104	58-59	0.7076917224693275
1104	60-61	0.751422222667351
1104	62-63	0.6749362906655847
1104	64-65	0.5933519892113424
1104	66-67	0.6652320341651219
1104	68-69	0.7108927083640921
1104	70-71	0.664118406527443
1104	72-73	0.6045150210526415
1104	74-75	0.5777796866129634
1104	76-77	0.5551754900154506
1104	78-79	0.3549998784199957
1104	80-81	0.34178509056879847
1104	82-83	0.4167730799346998
1104	84-85	0.48626859781244747
1104	86-87	0.4839021026488801
1104	88-89	0.5507574575547913
1104	90-91	0.5076902781723547
1104	92-93	0.3019850363367347
1104	94-95	0.48804645736524677
1104	96-97	0.29971782172024675
1104	98-99	0.3668066256647009
1104	100-101	0.3061697930253864
1105	1	0.07690890127891237
1105	2	0.2463548237797255
1105	3	0.23890168639070453
1105	4	0.23192879986418546
1105	5	0.3308780831770548
1105	6	0.28716233625797116
1105	7	0.276971650502837
1105	8	0.36180476198232725
1105	9	0.3577258835352737
1105	10-11	0.3140576609521659
1105	12-13	0.2826811176930164
1105	14-15	0.4073377837462786
1105	16-17	0.39187167373943765
1105	18-19	0.3989119885082175
1105	20-21	0.40558237898390814
1105	22-23	0.16961481330368855
1105	24-25	0.2841637498446161
1105	26-27	0.3302457534243004
1105	28-29	0.21890589385283477
1105	30-31	0.3613712187735274
1105	32-33	0.32717688985364646
1105	34-35	0.33767990466993325
1105	36-37	0.36261784742337966
1105	38-39	0.38929639483112055
1105	40-41	0.4157386826130036
1105	42-43	0.31148284238935275
1105	44-45	0.42766350722651936
1105	46-47	0.3654880551575346
1105	48-49	0.42882895266725995
1105	50-51	0.30372146870874417
1105	52-53	0.4136612727819511
1105	54-55	0.3685819839468323
1105	56-57	0.502973930870688
1105	58-59	0.4719036577243614
1105	60-61	0.556569681010302
1105	62-63	0.49299738003304583
1105	64-65	0.6171522264823963
1105	66-67	0.5901652515062281
1105	68-69	0.5362157449402147
1105	70-71	0.4121285348962189
1105	72-73	0.21663401528807213
1105	74-75	0.34536702685688425
1105	76-77	0.5370843061096338
1105	78-79	0.5966461965186767
1105	80-81	0.5875094437858692
1105	82-83	0.5864287392841092
1105	84-85	0.6572533510225398
1105	86-87	0.6720833293351554
1105	88-89	0.609140274571967
1105	90-91	0.5356051652571381
1105	92-93	0.5210549682510219
1105	94-95	0.6735308257294434
1105	96-97	0.5937834607083552
1105	98-99	0.6314598952688826
1105	100-101	0.6363227386776273
1106	1	0.17944485479306138
1106	2	0.205490105081914
1106	3	0.2030919134457676
1106	4	0.1429723273910497
1106	5	0.10945087404098075
1106	6	0.26950056762657937
1106	7	0.26559526728560456
1106	8	0.2814077560583996
1106	9	0.4287734533749159
1106	10-11	0.3042428171836278
1106	12-13	0.31164455026662097
1106	14-15	0.349877166875153
1106	16-17	0.33130525680326883
1106	18-19	0.2628207293744822
1106	20-21	0.3845411509427308
1106	22-23	0.4133557197344331
1106	24-25	0.4171597630109005
1106	26-27	0.3047216490795819
1106	28-29	0.3858938534020595
1106	30-31	0.3458880774033517
1106	32-33	0.3654727598764822
1106	34-35	0.48408924188941427
1106	36-37	0.41747178898655335
1106	38-39	0.46585058844109994
1106	40-41	0.365439721453761
1106	42-43	0.43936058521719445
1106	44-45	0.5083742407064094
1106	46-47	0.551589485452574
1106	48-49	0.6090207421023663
1106	50-51	0.631829402943076
1106	52-53	0.6931785152820495
1106	54-55	0.6741376796010243
1106	56-57	0.5959338620990664
1106	58-59	0.6351974029178038
1106	60-61	0.47436764420022826
1106	62-63	0.4581252405904408
1106	64-65	0.6148504992155992
1106	66-67	0.6586745141745567
1106	68-69	0.6175204966564749
1106	70-71	0.5974968757042802
1106	72-73	0.4686125349908892
1106	74-75	0.53547509147635
1106	76-77	0.5426861969556
1106	78-79	0.5351201038940445
1106	80-81	0.5718676173553803
1106	82-83	0.6517743878483415
1106	84-85	0.6134875630732282
1106	86-87	0.574740552232651
1106	88-89	0.6848109127549016
1106	90-91	0.6111693284731743
1106	92-93	0.6579273240059322
1106	94-95	0.676711718665878
1106	96-97	0.673224620306673
1106	98-99	0.7180942794727798
1106	100-101	0.6821103368097212
1107	1	0.15969014623242828
1107	2	0.32700329348289614
1107	3	0.3492566976377276
1107	4	0.3164134104430758
1107	5	0.25188730590078023
1107	6	0.34656828290840735
1107	7	0.2824671149911069
1107	8	0.2675354842224067
1107	9	0.3202648892623685
1107	10-11	0.3688272682115894
1107	12-13	0.33738466193321415
1107	14-15	0.4083522792776435
1107	16-17	0.45851967692350826
1107	18-19	0.34257338454214903
1107	20-21	0.31538443868203814
1107	22-23	0.3809994493290816
1107	24-25	0.4535426025012512
1107	26-27	0.4191293658806856
1107	28-29	0.5045813515227451
1107	30-31	0.47414323937213965
1107	32-33	0.5102057080107016
1107	34-35	0.5655624544088695
1107	36-37	0.46920512564329897
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2316	78-79	-0.3202981669651166
2316	80-81	-0.37464312024689406
2316	82-83	-0.32412608851524993
2316	84-85	-0.2259687050079222
2316	86-87	-0.23404504685749927
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2316	90-91	-0.22853142942038218
2316	92-93	-0.3036370627652687
2316	94-95	-0.2745033037229021
2316	96-97	-0.23952228550508536
2316	98-99	-0.13458065907848749
2316	100-101	-0.24429829180379414
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16438.0
3	2273.0
4	1886.0
5	1830.0
6	2007.0
7	2375.0
8	2738.0
9	3084.0
10	3420.0
11	4093.0
12	4782.0
13	5482.0
14	6150.0
15	7001.0
16	8342.0
17	10082.0
18	9823.0
19	7026.0
20	6871.0
21	7359.0
22	7881.0
23	9013.0
24	10349.0
25	12080.0
26	14251.0
27	17284.0
28	20845.0
29	25698.0
30	31965.0
31	40309.0
32	52763.0
33	70259.0
34	102264.0
35	164315.0
36	297180.0
37	577397.0
38	721191.0
39	204383.0
40	150.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.71260867469105	19.21569415205618	13.953629453452681	24.118067719800088
2	16.323495202268873	20.90246280718798	38.52222729791092	24.25181469263223
3	18.716903237833066	24.61661891854214	30.251419889012226	26.415057954612564
4	13.823824471309894	17.583844945157328	36.53967069085977	32.052659892673006
5	14.705734660042493	36.48736756643623	34.32030392882724	14.48659384469404
6	31.67427375226124	35.72093000838627	17.99296645813452	14.611829781217969
7	27.204724599214014	32.00270886019004	22.527562572099704	18.265003968496245
8	25.27848074320973	33.747588709194815	21.29659268493792	19.677337862657534
9	25.966636705888778	16.032148798235447	21.08371266248501	36.917501833390766
10-11	24.58151764594151	25.7870745077189	28.77187282963322	20.859535016706367
12-13	25.058278147823266	23.95529090073677	28.313480205511805	22.672950745928162
14-15	23.028952048408343	25.780704231632456	25.769476279293606	25.420867440665596
16-17	22.602464828624665	28.209455794830095	26.655860878117295	22.532218498427945
18-19	22.557586677553775	27.577527963498056	28.048907623285956	21.81597773566221
20-21	23.362899437152063	26.793413737121107	27.751659189276424	22.092027636450403
22-23	23.32755042935953	26.546240603617882	27.456555917160003	22.669653049862585
24-25	23.125632433446263	26.77571849158586	27.472175940549903	22.626473134417974
26-27	22.92302307213272	26.831041395851074	27.584321255886817	22.661614276129384
28-29	22.973775476985235	26.776121447250013	27.620770354024334	22.629332721740422
30-31	23.218671615155277	26.633783394918346	27.417001801464586	22.73054318846179
32-33	23.07637162778425	26.866257580996834	27.198855556131207	22.85851523508771
34-35	23.339987142262373	26.78016502169166	27.211266768331566	22.668581067714406
36-37	23.38236354445105	26.54297345331097	27.249635923719417	22.825027078518563
38-39	23.213999910436172	26.69247015225248	27.21997297421586	22.87355696309549
40-41	23.30327310174919	26.57844330761887	27.32800782720073	22.79027576343121
42-43	23.375075555698356	26.65960476086245	26.976587272102933	22.988732411336265
44-45	23.38744646780396	26.814872746867703	26.854820423244526	22.94286036208381
46-47	23.375760027217783	26.689613259269045	26.888484621110088	23.046142092403084
48-49	23.316602536856898	26.66947745118756	26.874742389389134	23.139177622566407
50-51	23.26960328976768	26.829782640349865	26.781078814840104	23.119535255042354
52-53	23.310553893613438	26.946615946919778	26.618167900087215	23.124662259379576
54-55	23.296996104574113	26.848801252961728	26.699168708084013	23.155033934380146
56-57	23.301675745403344	26.91634046075919	26.65376905646008	23.128214737377384
58-59	23.285564879603577	26.74953752382666	26.825766770635113	23.13913082593465
60-61	23.38779087116665	26.77004736351913	26.78848190543281	23.053679859881417
62-63	23.288138329724724	26.860101924713547	26.74487534926424	23.106884396297488
64-65	23.267548466945744	26.78991570983423	26.722730225273235	23.219805597946795
66-67	23.17006085989531	26.82790063587273	26.74071692285878	23.261321581373174
68-69	23.298047503112095	26.76371240729486	26.67393458631304	23.264305503280003
70-71	23.2575008600211	26.634968889627913	26.74383460765064	23.363695642700343
72-73	23.251584922272134	26.75064425122263	26.64844367541306	23.349327151092176
74-75	23.192511867868586	26.876540835065843	26.619545910710578	23.311401386354994
76-77	23.22124450473252	26.818411598822827	26.59624101511591	23.364102881328733
78-79	23.194665649069602	26.70993462608329	26.644661130135265	23.450738594711844
80-81	23.21454537566891	26.857416692307627	26.502587260283768	23.425450671739696
82-83	23.243117627894964	26.82318436775293	26.53592258594253	23.39777541840958
84-85	23.31046070347496	26.788391152899436	26.50316277739574	23.39798536622986
86-87	23.28357987116844	26.76885297893105	26.572377920638296	23.37518922926221
88-89	23.197390678636832	26.683347461601137	26.591508700181286	23.52775315958074
90-91	23.228031669832298	26.740124567257222	26.569005765619057	23.462837997291423
92-93	23.267616701457353	26.654586344374753	26.677284655299033	23.40051229886886
94-95	23.18108790913071	26.617424379350375	26.676366513638023	23.525121197880893
96-97	23.157008484057958	26.58633330147361	26.73132852653045	23.525329687937987
98-99	23.123752006027317	26.597833892102642	26.6854444746096	23.592969627260445
100-101	23.259470562533586	26.598191013323824	26.638167509554684	23.504170914587906
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4894.0
1	2832.5
2	758.0
3	679.0
4	521.5
5	478.0
6	650.5
7	1028.0
8	1512.5
9	1636.0
10	1226.0
11	883.5
12	863.0
13	891.0
14	957.5
15	1043.5
16	1113.5
17	1219.0
18	1319.0
19	1455.0
20	1636.0
21	1798.5
22	2015.5
23	2389.5
24	2927.5
25	3769.5
26	5231.5
27	7374.5
28	10916.5
29	16153.5
30	22673.5
31	30032.0
32	38212.0
33	45770.5
34	51804.0
35	56496.0
36	60754.0
37	64471.5
38	66213.0
39	67397.5
40	69997.5
41	73140.5
42	76175.0
43	79769.5
44	83856.0
45	88678.0
46	92898.5
47	95103.0
48	97034.5
49	98875.5
50	98140.5
51	96323.0
52	95342.5
53	94238.5
54	90727.5
55	85353.0
56	78577.5
57	71053.5
58	64980.5
59	58438.0
60	49696.5
61	40725.0
62	32917.5
63	25202.0
64	18418.5
65	13511.5
66	9971.0
67	7492.0
68	5633.0
69	4391.0
70	3514.5
71	2693.0
72	1996.5
73	1523.5
74	1202.5
75	881.5
76	632.5
77	459.5
78	318.0
79	255.5
80	207.0
81	163.5
82	130.0
83	92.5
84	76.5
85	53.5
86	39.0
87	36.0
88	30.5
89	27.0
90	17.0
91	11.5
92	12.5
93	11.5
94	10.5
95	8.0
96	6.5
97	4.5
98	4.5
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14278040261746688
2	0.1464311518836061
3	0.14185768576998112
4	0.14482642693145698
5	0.1352783134661698
6	0.11453724345964257
7	0.12159803324909865
8	0.11192956541240028
9	0.10900094237472815
10-11	0.09710591866692289
12-13	0.11453724345964257
14-15	0.13303169853316105
16-17	0.1420582763889998
18-19	0.13866829492758478
20-21	0.13020337080499822
22-23	0.12504819189621924
24-25	0.1104251357697605
26-27	0.11475789314056307
28-29	0.11897029613995448
30-31	0.1091413558080412
32-33	0.10815846177484986
34-35	0.12386470724400926
36-37	0.12514848720572855
38-39	0.11211009696951704
40-41	0.11473783407866121
42-43	0.11048531295546607
44-45	0.125670022815177
46-47	0.12396500255351858
48-49	0.13708362903733753
50-51	0.12442636097726145
52-53	0.11022454515074184
54-55	0.10185991633766463
56-57	0.12157797418719679
58-59	0.11156850229816673
60-61	0.1102446042126437
62-63	0.11080625794589591
64-65	0.13086531984775976
66-67	0.12310246289173844
68-69	0.12677327121977952
70-71	0.11531954687381525
72-73	0.11742574837351097
74-75	0.10077672699496398
76-77	0.124787424091495
78-79	0.12492783752480804
80-81	0.12569008187707886
82-83	0.13126650108579704
84-85	0.1225608682203881
86-87	0.13096561515726907
88-89	0.11832840615909485
90-91	0.12599096780560684
92-93	0.13908953522752393
94-95	0.11670362214504387
96-97	0.10258204256613171
98-99	0.10603220121325228
100-101	0.14749428216440488
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2492639.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.45324926649467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.82816732417042	28.390409371914103
2	19.535153887891855	18.540130538029292
3	8.780362140645085	12.49970139930372
4	4.315251506266917	8.190908214980023
5	2.396822517598744	5.6868508187580264
6	1.4517236881052336	4.133340362263957
7	0.9451045532375891	3.1393797363377685
8	0.6143570936298333	2.3322592242124545
9	0.4182918750976269	1.7864377753641432
>10	1.6590655931710614	12.724557336443379
>50	0.04703175479570458	1.4686106544833606
>100	0.008159915915677583	0.5704104044407915
>500	8.469157904516722E-5	0.02700694490728656
>1k	4.2345789522581147E-4	0.5099972185616762
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3945	0.15826599840570577	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3239	0.12994260300027402	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	4.01181238037277E-5	0.0
9	0.0	0.0	0.0	4.01181238037277E-5	0.0
10-11	4.01181238037277E-5	0.0	0.0	4.01181238037277E-5	0.0
12-13	4.01181238037277E-5	0.0	0.0	4.01181238037277E-5	0.0
14-15	4.01181238037277E-5	0.0	0.0	1.0029530950931924E-4	0.0
16-17	6.0177185705591546E-5	0.0	0.0	1.604724952149108E-4	0.0
18-19	8.02362476074554E-5	0.0	0.0	1.604724952149108E-4	0.0
20-21	8.02362476074554E-5	0.0	0.0	1.604724952149108E-4	0.0
22-23	8.02362476074554E-5	0.0	0.0	1.8053155711677464E-4	0.0
24-25	1.2035437141118309E-4	0.0	0.0	4.01181238037277E-4	0.0
26-27	1.604724952149108E-4	0.0	0.0	8.424805998782816E-4	0.0
28-29	1.604724952149108E-4	0.0	0.0	0.002266673994910615	0.0
30-31	1.604724952149108E-4	0.0	0.0	0.006077895756264746	0.0
32-33	1.604724952149108E-4	0.0	0.0	0.015726304531061257	0.0
34-35	1.604724952149108E-4	0.0	0.0	0.03022900628610882	0.0
36-37	1.604724952149108E-4	0.0	0.0	0.04974647351662234	0.0
38-39	1.604724952149108E-4	0.0	0.0	0.07157073286585021	0.0
40-41	1.604724952149108E-4	0.0	0.0	0.09558142996238123	0.0
42-43	1.604724952149108E-4	0.0	0.0	0.12061513921590733	0.0
44-45	1.8053155711677464E-4	0.0	0.0	0.15449489476815537	0.0
46-47	2.2064968092050234E-4	0.0	0.0	0.19136345054378112	0.0
48-49	2.4070874282236619E-4	0.0	0.0	0.22608568669590742	0.0
50-51	2.6076780472423003E-4	0.0	0.0	0.262833888100122	0.0
52-53	2.808268666260939E-4	0.0	0.0	0.300845810404154	0.0
54-55	2.808268666260939E-4	0.0	0.0	0.3439928525550632	0.0
56-57	2.808268666260939E-4	0.0	0.0	0.3983930284329179	0.0
58-59	2.808268666260939E-4	0.0	0.0	0.45044629406825454	0.0
60-61	2.808268666260939E-4	0.0	0.0	0.5047662336985018	0.0
62-63	2.808268666260939E-4	0.0	0.0	0.5658059590658735	0.0
64-65	3.209449904298216E-4	0.0	0.0	0.6337259426655846	0.0
66-67	3.209449904298216E-4	0.0	0.0	0.718074297962922	0.0
68-69	3.209449904298216E-4	0.0	0.0	0.8108073411352386	0.0
70-71	3.209449904298216E-4	0.0	0.0	0.8993279813081638	0.0
72-73	3.209449904298216E-4	0.0	0.0	0.9924421466566158	0.0
74-75	3.209449904298216E-4	0.0	0.0	1.1014631480932457	0.0
76-77	3.209449904298216E-4	0.0	0.0	1.2221785826186624	0.0
78-79	4.01181238037277E-4	0.0	0.0	1.3532444930854408	0.0
80-81	4.01181238037277E-4	0.0	0.0	1.4908496577322268	2.005906190186385E-5
82-83	4.01181238037277E-4	0.0	0.0	1.6477917580524095	4.01181238037277E-5
84-85	4.01181238037277E-4	0.0	0.0	1.836467294301341	4.01181238037277E-5
86-87	4.01181238037277E-4	0.0	0.0	2.043316340633361	4.01181238037277E-5
88-89	4.01181238037277E-4	0.0	0.0	2.293613315044818	4.01181238037277E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3025	0.0	55.595543	1
GTATCAA	5145	0.0	39.42799	1
TCAACGC	6070	0.0	32.636944	4
ATCAACG	6300	0.0	31.520845	3
CAACGCA	6440	0.0	30.761839	5
TATCAAC	6555	0.0	30.584534	2
AACGCAG	6770	0.0	29.325474	6
GTGGTAT	1465	0.0	26.266941	1
ACGCAGA	7500	0.0	26.091684	7
CGCAGAG	7900	0.0	24.41181	8
GTACATG	6460	0.0	22.57709	1
TGGTATC	1670	0.0	21.335701	2
GCAGAGT	8845	0.0	20.675877	9
TACATGG	6885	0.0	20.56243	2
CAGAGTA	7995	0.0	20.26192	10-11
ACATGGG	7285	0.0	19.62904	3
GAGTACT	4380	0.0	19.090345	12-13
AGAGTAC	7435	0.0	18.209942	10-11
GTACTTT	4820	0.0	16.805712	14-15
CATGGGG	4835	0.0	16.310743	4
>>END_MODULE