FastQCFastQC Report
Fri 10 Feb 2017
SRR2031772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492639
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT69410.278459897321674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51140.20516408513226342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42740.17146486113713216No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT34530.13852788149427173RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT25480.10222097945189818RNA PCR Primer, Index 26 (100% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32450.061.201171
GTATCAA56050.039.6705671
TCAACGC67850.032.065954
ATCAACG69800.031.170753
CAACGCA70050.031.0538965
TATCAAC70150.031.0152262
AACGCAG72800.029.8808446
ACGCAGA84050.025.7683077
CGCAGAG87150.024.7972078
GTACATG59500.022.5180261
GCAGAGT98500.021.7469869
GTGGTAT15100.021.7105241
CAGAGTA90400.021.19990310-11
GAGTACT56300.021.0906712-13
CGTATAC2056.82925E-720.855753
TACATGG63200.020.6705862
GTACTTT60700.019.44448714-15
GTATACG1507.7120203E-419.0045741
ACATGGG65250.018.8562983
AGAGTAC84600.018.58302510-11
Produced by FastQC (version 0.11.5)