FastQCFastQC Report
Fri 10 Feb 2017
SRR2031770_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031770_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572229
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10620.1855900347588116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8300.1450468256589582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6450.044.9319231
GTATCAA11750.040.0296861
ATCAACG12300.037.853483
TCAACGC12300.037.467224
CAACGCA12200.037.3849075
AACGCAG13050.035.304666
TATCAAC13600.034.2351342
ACGCAGA15250.030.2115297
CGCAGAG15650.029.4419258
GGACCGG650.00584314329.2292376
GTACATG10550.022.966911
GCAGAGT18900.022.8711729
GTATAGT2303.608875E-922.7221641
GAGTACT11750.021.63220412-13
TACATGG10900.020.9218252
GTATAGG1604.9755916E-520.7856161
GTGGTAT3451.8189894E-1220.6565151
CATGGGG7100.020.074644
CAGAGTA17050.020.0611110-11
GGGCGCG1200.00476206619.7906287
Produced by FastQC (version 0.11.5)