FastQCFastQC Report
Fri 10 Feb 2017
SRR2031770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572229
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT48120.840922078398683No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT24890.43496572176523735RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT18580.32469518322210167RNA PCR Primer, Index 26 (100% over 24bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG14530.2539193225089955RNA PCR Primer, Index 26 (100% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13440.23487100444052994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10920.19083269110793055No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT8270.14452256002404631RNA PCR Primer, Index 26 (100% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.10485312698237943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8600.054.6935651
GTATCAA14450.038.1407851
TCAACGC16700.032.7146954
CAACGCA17200.031.7553625
ATCAACG17250.031.6716183
AACGCAG17500.031.2109836
TATCAAC17700.031.1348132
ACGCAGA19400.027.66467
CGCAGAG20000.026.5971858
GTGGTAT3750.024.0725361
TATACAC21550.023.147473
CTTATAC21300.022.9750821
TTATACA21750.022.2793462
TACACAT23700.021.8436815
ACACATC23650.020.885746
GCAGAGT24850.020.641689
ATTATAC2108.5792817E-720.3603293
ACATCTC24350.020.2853288
GAGTACT15150.020.22063312-13
TGGTATC4000.020.1906572
Produced by FastQC (version 0.11.5)