##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031770_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 572229 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66352806306566 34.0 31.0 34.0 31.0 34.0 2 32.83620718278871 34.0 31.0 34.0 31.0 34.0 3 32.89816140041837 34.0 31.0 34.0 31.0 34.0 4 36.31518150950057 37.0 37.0 37.0 35.0 37.0 5 36.277271861440084 37.0 37.0 37.0 35.0 37.0 6 36.29918966008364 37.0 37.0 37.0 35.0 37.0 7 36.24166373951687 37.0 37.0 37.0 35.0 37.0 8 36.23010542981918 37.0 37.0 37.0 35.0 37.0 9 38.07561133741911 39.0 39.0 39.0 35.0 39.0 10-11 38.030465949820794 39.0 38.5 39.0 35.0 39.0 12-13 37.97595717798294 39.0 38.0 39.0 35.0 39.0 14-15 39.44279999790294 41.0 39.0 41.0 36.0 41.0 16-17 39.40426385240874 41.0 39.0 41.0 36.0 41.0 18-19 39.40912991127678 41.0 39.0 41.0 36.0 41.0 20-21 39.40209601400838 41.0 39.0 41.0 36.0 41.0 22-23 39.3021264214152 41.0 39.0 41.0 36.0 41.0 24-25 39.22632285326329 41.0 39.0 41.0 36.0 41.0 26-27 39.08043108615607 40.5 39.0 41.0 35.5 41.0 28-29 38.96255694835459 40.0 39.0 41.0 35.0 41.0 30-31 38.793928654437295 40.0 38.0 41.0 35.0 41.0 32-33 38.56758570432467 40.0 38.0 41.0 34.5 41.0 34-35 38.39934012432086 40.0 38.0 41.0 34.0 41.0 36-37 38.305895017554164 40.0 38.0 41.0 34.0 41.0 38-39 38.34477805214346 40.0 38.0 41.0 34.0 41.0 40-41 38.278291558099994 40.0 38.0 41.0 34.0 41.0 42-43 38.17970427923087 40.0 38.0 41.0 34.0 41.0 44-45 37.99236756613175 40.0 38.0 41.0 33.0 41.0 46-47 37.79246944842013 40.0 38.0 41.0 33.0 41.0 48-49 37.579351972724204 40.0 37.5 41.0 33.0 41.0 50-51 37.40519005503042 40.0 37.0 41.0 32.5 41.0 52-53 37.50626846944143 40.0 37.0 41.0 33.0 41.0 54-55 37.41329258041798 40.0 37.0 41.0 33.0 41.0 56-57 37.17934865237518 40.0 37.0 41.0 32.0 41.0 58-59 36.93769190306678 40.0 36.0 41.0 32.0 41.0 60-61 36.60353372513452 39.0 35.5 41.0 31.0 41.0 62-63 36.26411891043621 39.0 35.0 41.0 31.0 41.0 64-65 35.85376047002162 39.0 35.0 41.0 30.0 41.0 66-67 35.39326213806011 38.0 35.0 40.5 29.5 41.0 68-69 34.916722151446365 37.0 35.0 40.0 28.5 41.0 70-71 34.55713883777299 37.0 35.0 39.5 28.0 41.0 72-73 34.17477093960635 36.0 34.5 39.0 28.0 41.0 74-75 33.796459109901804 36.0 34.0 39.0 27.0 40.5 76-77 32.86711351574283 35.0 33.0 37.0 26.0 39.0 78-79 33.069723834339044 35.0 34.0 37.0 26.0 39.0 80-81 32.84383524777668 35.0 34.0 37.0 26.0 39.0 82-83 32.56922840331406 35.0 34.0 36.0 26.0 38.0 84-85 32.26355794620685 35.0 34.0 36.0 26.0 37.0 86-87 32.014385849021984 35.0 34.0 35.5 26.0 37.0 88-89 31.784606337672507 35.0 33.0 35.0 25.0 36.0 90-91 31.576167932768175 35.0 33.0 35.0 25.0 36.0 92-93 31.400262831838305 35.0 33.0 35.0 24.0 36.0 94-95 31.218367996029563 35.0 33.0 35.0 23.5 36.0 96-97 31.046080327980583 35.0 33.0 35.0 21.5 35.0 98-99 30.82891377403103 35.0 33.0 35.0 19.0 35.0 100-101 30.100470266274513 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14970850789524803 1101 2 0.2439829852321509 1101 3 0.18676640048981596 1101 4 0.17482611893234434 1101 5 0.16227519494361076 1101 6 0.02502242981087477 1101 7 0.25170475154448724 1101 8 0.18777337278349648 1101 9 0.12053438811840778 1101 10-11 0.2695154337968475 1101 12-13 0.3331316030423679 1101 14-15 0.22085783435414186 1101 16-17 0.31138685934462984 1101 18-19 0.3679054148800063 1101 20-21 0.285679835211198 1101 22-23 0.2284146198515984 1101 24-25 0.2017903971863575 1101 26-27 0.22876381925538425 1101 28-29 0.212987952853112 1101 30-31 0.17624316360136305 1101 32-33 0.2804931418953345 1101 34-35 0.18318324192063784 1101 36-37 0.1426088318168155 1101 38-39 0.25091680947159745 1101 40-41 0.09520674281529296 1101 42-43 0.13033002685694584 1101 44-45 0.13043667158283512 1101 46-47 0.22636150123711474 1101 48-49 0.2484988760052005 1101 50-51 0.4088888407743596 1101 52-53 0.3537492169623917 1101 54-55 0.27345481663915905 1101 56-57 0.1639002697885985 1101 58-59 0.5700319914010805 1101 60-61 0.34265594732073623 1101 62-63 0.42614438272494937 1101 64-65 0.34643238759508677 1101 66-67 0.19775657476476027 1101 68-69 -0.04401657514821977 1101 70-71 0.16224664780092724 1101 72-73 0.08577729475669571 1101 74-75 0.042238002190359225 1101 76-77 -0.044476820134796924 1101 78-79 -0.14620911705980433 1101 80-81 0.10640685936961347 1101 82-83 0.028074323696429815 1101 84-85 -0.23823961244168146 1101 86-87 0.05953043434517724 1101 88-89 0.013568463744899617 1101 90-91 -0.22144642377118018 1101 92-93 -0.23340995300517875 1101 94-95 -0.25874166292048173 1101 96-97 -0.04500064234511214 1101 98-99 -0.2772261350147609 1101 100-101 -0.5699935826057292 1102 1 0.17011394128956425 1102 2 0.1522254167969308 1102 3 0.13566004542060028 1102 4 0.14895794950985675 1102 5 0.1057061038462308 1102 6 0.18102971345891206 1102 7 0.1319862593631953 1102 8 0.21960049833149498 1102 9 -0.018994073340387274 1102 10-11 0.22532164043152392 1102 12-13 0.12877306460472937 1102 14-15 0.1338855536402761 1102 16-17 0.1461951889383286 1102 18-19 0.20892728308394481 1102 20-21 0.18399354987169403 1102 22-23 0.22758183307952606 1102 24-25 0.10831547571857669 1102 26-27 0.056745886292567604 1102 28-29 -0.0860903722530395 1102 30-31 -0.2464932097632655 1102 32-33 -0.15838117202645208 1102 34-35 -0.3205125190652467 1102 36-37 -0.15617176123092946 1102 38-39 -0.21009755070652147 1102 40-41 -0.08285860289909408 1102 42-43 -0.07974151513621308 1102 44-45 -0.45494338986215865 1102 46-47 -0.4256350295371547 1102 48-49 -0.39147834101989787 1102 50-51 -0.23556514452025823 1102 52-53 -0.3782940879497616 1102 54-55 -0.14359875787152276 1102 56-57 -0.1815401147604021 1102 58-59 -0.3071794242501369 1102 60-61 -0.26419318450984264 1102 62-63 -0.20195591414412206 1102 64-65 -0.4078653099436593 1102 66-67 -0.20115907644860442 1102 68-69 -0.3084544225022938 1102 70-71 -0.2405222603422601 1102 72-73 -0.345716284586743 1102 74-75 -0.44394334683791215 1102 76-77 -0.594806482877722 1102 78-79 -0.3829600404606808 1102 80-81 -0.4318171904767709 1102 82-83 -0.19877808750174353 1102 84-85 -0.2519137123415831 1102 86-87 -0.20975742509077122 1102 88-89 -0.5183773231307249 1102 90-91 -0.5585006886750428 1102 92-93 -0.35485442813376267 1102 94-95 -0.24295323391016055 1102 96-97 -0.3844108739720262 1102 98-99 -0.503293223971923 1102 100-101 -0.5704703853949162 1103 1 0.26251462861125674 1103 2 0.07180388126150206 1103 3 0.134713141429323 1103 4 0.21733911101821235 1103 5 0.12926850359480824 1103 6 0.08895962623762443 1103 7 0.05350750960298001 1103 8 0.13587296179202468 1103 9 0.2700197996193765 1103 10-11 0.27290356599344534 1103 12-13 0.1687838397191186 1103 14-15 0.23435941378419045 1103 16-17 0.2912470033614767 1103 18-19 0.24644427225577203 1103 20-21 0.18489351681169097 1103 22-23 0.1322179893023545 1103 24-25 -0.0660291401934856 1103 26-27 0.203350704891065 1103 28-29 0.31683972419239836 1103 30-31 0.25279457150191575 1103 32-33 0.2120766478903704 1103 34-35 0.1595576008079007 1103 36-37 0.1811138078706236 1103 38-39 0.2638197339646524 1103 40-41 0.032543280297034016 1103 42-43 0.20016248839018402 1103 44-45 0.156764751471421 1103 46-47 0.2778309655895512 1103 48-49 0.34071721098023744 1103 50-51 0.16629560345226224 1103 52-53 0.23153657133214978 1103 54-55 0.4868944076856536 1103 56-57 0.29611172660052887 1103 58-59 0.331693025333351 1103 60-61 0.20706909565129905 1103 62-63 0.03460070214344313 1103 64-65 0.12290906977300153 1103 66-67 0.00295383368939639 1103 68-69 0.12277463438950065 1103 70-71 -0.06700210393984207 1103 72-73 0.07524988400298582 1103 74-75 0.3310465514923493 1103 76-77 0.14456227500463825 1103 78-79 0.2484637031568937 1103 80-81 0.039669571234021817 1103 82-83 0.09617026133441442 1103 84-85 0.0560566071160693 1103 86-87 0.19464812735832027 1103 88-89 0.08529029724240544 1103 90-91 -0.14944224592396083 1103 92-93 -0.046192297354892276 1103 94-95 -0.2673054005652631 1103 96-97 -0.15090312527707894 1103 98-99 0.24228959135322725 1103 100-101 0.11502688205240119 1104 1 0.2302935599679472 1104 2 0.08171527030464176 1104 3 0.1870386760326639 1104 4 0.053791131525088076 1104 5 0.03374412149728556 1104 6 0.029523484878602346 1104 7 0.17663327921449223 1104 8 0.03424400105931369 1104 9 0.07802536899105661 1104 10-11 0.11091493008709108 1104 12-13 0.15205560413146912 1104 14-15 0.14943655602046846 1104 16-17 -4.74994851821009E-4 1104 18-19 0.19764386018665192 1104 20-21 0.16608280301461775 1104 22-23 0.17434239876384794 1104 24-25 0.018710620452303317 1104 26-27 0.023259598984466834 1104 28-29 0.04843142164352798 1104 30-31 0.1435700984594419 1104 32-33 0.22176671074700494 1104 34-35 0.08947365782152161 1104 36-37 0.2685617962167868 1104 38-39 0.07137661479458757 1104 40-41 0.049319805234461 1104 42-43 0.04844741165035771 1104 44-45 0.08890614268759833 1104 46-47 -0.11345964776567286 1104 48-49 -0.011694439630225872 1104 50-51 0.04309941187231203 1104 52-53 0.22615834499996623 1104 54-55 -0.028183941784362787 1104 56-57 -0.014010525936676288 1104 58-59 0.20087315265627126 1104 60-61 0.40062085781014645 1104 62-63 0.24427708301764284 1104 64-65 0.12212839195149172 1104 66-67 -0.045168515426517786 1104 68-69 -0.04357412739109634 1104 70-71 0.04765693300955576 1104 72-73 0.17133562979175565 1104 74-75 0.1899772643236446 1104 76-77 0.11066918762505651 1104 78-79 0.1629895419831442 1104 80-81 0.16115551500912062 1104 82-83 0.050532801676148154 1104 84-85 -0.12584937351989112 1104 86-87 -0.22743097059662531 1104 88-89 -0.11360548629253842 1104 90-91 -0.1825365469758644 1104 92-93 -0.20396165132387623 1104 94-95 0.01493780069669981 1104 96-97 0.11211085448968205 1104 98-99 0.1573043598460373 1104 100-101 0.38169878006529245 1105 1 0.026753531126715302 1105 2 0.09346109530348201 1105 3 0.08431891668639935 1105 4 0.017514751875218337 1105 5 0.07106715882031978 1105 6 0.041439876313070556 1105 7 0.06892057511624472 1105 8 -0.04247988289348825 1105 9 -0.05883868955974947 1105 10-11 0.03590834002026355 1105 12-13 -0.05074788782864914 1105 14-15 0.09833640251067521 1105 16-17 0.012817557597365692 1105 18-19 0.033605467232597164 1105 20-21 0.04780273292731607 1105 22-23 0.1455748187914523 1105 24-25 0.1824117570450028 1105 26-27 0.17829673594931705 1105 28-29 0.11158752094420521 1105 30-31 0.10700685707692514 1105 32-33 -0.03442407556426019 1105 34-35 -0.027441880780763483 1105 36-37 0.2941777736399942 1105 38-39 0.30898728915224893 1105 40-41 0.11219679582229958 1105 42-43 -0.3306713801190355 1105 44-45 -0.05447432900613336 1105 46-47 -0.007126981192037363 1105 48-49 0.1853485586175978 1105 50-51 0.1502383925052726 1105 52-53 0.19850332336903875 1105 54-55 0.15614410398946177 1105 56-57 0.1366073897414779 1105 58-59 0.43558807411817924 1105 60-61 0.391650613900147 1105 62-63 0.6389290499587688 1105 64-65 0.5840804472770458 1105 66-67 0.5557215312466681 1105 68-69 0.46542325208821467 1105 70-71 0.44476660722767036 1105 72-73 0.48227048891938296 1105 74-75 0.5227407507015869 1105 76-77 0.34764411435902076 1105 78-79 0.5028199059098952 1105 80-81 0.41708269864714964 1105 82-83 0.6016174491463389 1105 84-85 0.5863546549491971 1105 86-87 0.37845589733843354 1105 88-89 0.45585381810652237 1105 90-91 0.25043859684265257 1105 92-93 0.38877609502832655 1105 94-95 0.2788622242596759 1105 96-97 0.5833859161261934 1105 98-99 0.183660750660259 1105 100-101 0.1444680036778898 1106 1 0.04031413005316864 1106 2 0.09653251300904486 1106 3 0.08410159939590756 1106 4 0.10700963405302133 1106 5 0.10378789885813688 1106 6 0.13468710370663928 1106 7 0.07083296727253696 1106 8 0.14296782694446364 1106 9 0.16279597109929966 1106 10-11 0.1974656357238942 1106 12-13 0.0913776201505172 1106 14-15 0.08504201726507432 1106 16-17 0.10632556573951746 1106 18-19 0.041544792245097995 1106 20-21 0.12673780576482585 1106 22-23 0.09422455402656027 1106 24-25 0.2757839418806043 1106 26-27 0.08478148389747986 1106 28-29 0.2468385586342663 1106 30-31 0.15690109599555768 1106 32-33 0.05809513890617524 1106 34-35 0.27243062640881277 1106 36-37 0.31681861335400896 1106 38-39 0.06953623262802466 1106 40-41 0.03430665695654511 1106 42-43 0.10136263728083605 1106 44-45 0.24772528416908557 1106 46-47 0.20528437146321465 1106 48-49 0.094064430748098 1106 50-51 0.23469694647602068 1106 52-53 0.34610341609108275 1106 54-55 0.24544340060582925 1106 56-57 0.3336163794334013 1106 58-59 0.3983761100769456 1106 60-61 0.354876906394729 1106 62-63 0.2855566478058762 1106 64-65 0.17678981437666152 1106 66-67 0.11797750203921709 1106 68-69 0.2618313594719268 1106 70-71 0.02979561908594519 1106 72-73 -0.09252468953432924 1106 74-75 0.08073730260057488 1106 76-77 0.1893610658320739 1106 78-79 0.1504308644964638 1106 80-81 0.3482999652302752 1106 82-83 0.49341879216719065 1106 84-85 0.47262708390454833 1106 86-87 0.46977624475633917 1106 88-89 0.4646587903261299 1106 90-91 0.4403376891807689 1106 92-93 0.3831711199111574 1106 94-95 0.4635728662873895 1106 96-97 0.4309867332392052 1106 98-99 0.5095877900019339 1106 100-101 0.5722715778229279 1107 1 0.21917591867105557 1107 2 0.18471262557940094 1107 3 0.3123692666879663 1107 4 0.21301338639823797 1107 5 0.24715875564362477 1107 6 0.0723043644946273 1107 7 0.2368742430699129 1107 8 0.27351817090944053 1107 9 0.38934381350080116 1107 10-11 0.24507351564884772 1107 12-13 0.2880630485432647 1107 14-15 0.3572433931249819 1107 16-17 0.3614850404111891 1107 18-19 0.2451876940587283 1107 20-21 0.2558810202738968 1107 22-23 0.2914571994299351 1107 24-25 0.2112123346260688 1107 26-27 0.35823364149597836 1107 28-29 0.37848333662050493 1107 30-31 0.3671106019367656 1107 32-33 0.1892708737404405 1107 34-35 0.21684901290037573 1107 36-37 0.4307960992827091 1107 38-39 0.5171597473247047 1107 40-41 0.7032403655244011 1107 42-43 0.4554902170181734 1107 44-45 0.2948641209420728 1107 46-47 0.46880844901168217 1107 48-49 0.3305403531996305 1107 50-51 0.3838526688024757 1107 52-53 0.5796087319134742 1107 54-55 0.4745709803431666 1107 56-57 0.591668286875553 1107 58-59 0.5094611632351729 1107 60-61 0.45751918281749937 1107 62-63 0.3911695308577805 1107 64-65 0.33688987691574823 1107 66-67 0.5329243438153313 1107 68-69 0.4857994645375996 1107 70-71 0.5543422107057054 1107 72-73 0.39934522916485093 1107 74-75 0.5276572525693055 1107 76-77 0.4907994648314471 1107 78-79 0.27801060183229964 1107 80-81 0.2784907094453999 1107 82-83 0.26976338253616916 1107 84-85 0.4175926874067315 1107 86-87 0.3382096406287687 1107 88-89 0.3792929366675928 1107 90-91 0.4351782145090972 1107 92-93 0.5473362231006575 1107 94-95 0.513995005124162 1107 96-97 0.4595983655093754 1107 98-99 0.6086340689262641 1107 100-101 0.5390476878917205 1108 1 -0.023548664051809 1108 2 -0.0619477208827206 1108 3 -0.07534655393313727 1108 4 0.008755866791240408 1108 5 -0.0840415086558366 1108 6 -0.015022188982918294 1108 7 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-0.6619147597311326 2316 76-77 -1.165151712555975 2316 78-79 -0.8486164675472097 2316 80-81 -0.7427138484550966 2316 82-83 -0.6206753469806614 2316 84-85 -0.47587902580780295 2316 86-87 -0.4616450289023888 2316 88-89 -0.6625369634966916 2316 90-91 -0.7345424982420674 2316 92-93 -0.7147483213199237 2316 94-95 -0.4649636704729545 2316 96-97 -0.44404121492788207 2316 98-99 -0.5581221128842628 2316 100-101 -0.3151690019435698 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 3.0 5 5.0 6 15.0 7 30.0 8 81.0 9 233.0 10 404.0 11 635.0 12 901.0 13 1260.0 14 1562.0 15 1765.0 16 2252.0 17 2971.0 18 3006.0 19 2359.0 20 2108.0 21 2487.0 22 2957.0 23 3203.0 24 3841.0 25 4233.0 26 4091.0 27 3540.0 28 4309.0 29 5372.0 30 7035.0 31 9001.0 32 11519.0 33 15090.0 34 21455.0 35 33337.0 36 58760.0 37 119863.0 38 186891.0 39 55569.0 40 84.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.8071521581445 20.551618062170643 14.170257019759323 24.47097275992554 2 15.784589735927398 21.593278215539584 39.316078003736266 23.30605404479675 3 17.92932426221429 26.1176227982643 31.457012088029234 24.496040851492175 4 13.003611143155783 19.355972019758163 36.99586448949342 30.64455234759263 5 13.496344994748604 36.931717896156954 35.726605956706145 13.845331152388294 6 29.636212075934637 37.35287795620285 18.594828294266804 14.41608167359571 7 25.019528894900468 32.62452619493244 23.380150254530964 18.97579465563612 8 23.900396519575203 34.52341632458334 22.508471258884118 19.06771589695734 9 24.539651083744445 15.877734263730082 21.52180333397993 38.06081131854555 10-11 23.06779546981669 26.302167752916883 30.090514540956974 20.539522236309445 12-13 24.4091963182572 24.403342018667352 28.967336503392872 22.220125159682574 14-15 21.928546089065744 26.241155201851008 26.486249386172318 25.344049322910934 16-17 21.653902109756256 28.877840350562938 27.404811939411676 22.063445600269127 18-19 21.432503420833267 27.525518629779338 29.251837987938394 21.790139961449 20-21 22.08005885755528 26.809983415730414 28.836794360299812 22.273163366414494 22-23 21.679083024453497 27.52726618189571 28.657845023583217 22.13580577006758 24-25 22.029292468574642 27.51564496032183 28.870172605722534 21.584889965380995 26-27 21.759857958715756 27.379383742144743 29.209336138741897 21.651422160397605 28-29 21.322259165243075 26.97218205093477 29.60429218676954 22.101266597052614 30-31 21.946108987835288 26.715353468628816 28.792581291755575 22.545956251780318 32-33 22.248474384336316 26.83414652900592 29.283607233480364 21.633771853177404 34-35 22.073549369203572 27.634100495741237 28.94567819026921 21.346671944785978 36-37 21.858048122361097 27.16145177977239 28.703897883287194 22.276602214579313 38-39 21.78769338848608 26.951797269974083 29.10102424029541 22.15948510124443 40-41 21.971535871128516 26.77328482128658 29.28250752758074 21.97267178000416 42-43 22.27526042895414 26.76428492788726 29.14016940770216 21.820285235456435 44-45 21.915963713827853 27.115018637643324 28.9201525962508 22.04886505227802 46-47 22.477015320789402 26.641606769317878 28.352809801670308 22.52856810822241 48-49 22.177397510437256 26.7115088539728 28.611360137287694 22.499733498302252 50-51 22.071233719367594 26.980282369470963 28.669378867551277 22.279105043610162 52-53 21.97905034522892 27.304453287058156 28.940861088829823 21.775635278883104 54-55 22.05305917735732 27.329967547957203 28.273907823616067 22.343065451069414 56-57 22.350143037140725 27.887174540262727 27.758292571680222 22.00438985091633 58-59 21.654974035630996 28.363069518800682 28.419515698766133 21.562440746802185 60-61 22.304746958769282 28.688491643933528 27.50730052654665 21.499460870750532 62-63 21.639157744617936 29.14879635358228 27.456490812299666 21.75555508950012 64-65 21.770009865233398 29.02056320084444 27.439417068688766 21.770009865233398 66-67 21.682864298159917 28.896825077813293 27.429950559504228 21.990360064522555 68-69 21.78190483097655 28.679068214923277 27.46920511312648 22.06982184097369 70-71 21.942196168139127 28.145312580551302 27.670764388701368 22.241726862608207 72-73 21.954868295956604 28.18555659638723 27.577456518091832 22.28211858956434 74-75 22.00241699340351 28.130185591189072 27.53790861038608 22.329488805021334 76-77 21.973096129564986 28.08278248408617 27.527928384500782 22.416193001848058 78-79 22.048475644654076 27.917577947854472 27.44888160349373 22.585064803997724 80-81 22.007128869299112 27.843311682240135 27.577836015033718 22.571723433427042 82-83 22.120163205097843 27.740497252970524 27.52578589861115 22.613553643320483 84-85 22.211658945183 27.792972197416 27.328721996878784 22.666646860522217 86-87 21.996878784648374 27.74342770961163 27.58666790163803 22.67302560410197 88-89 22.070320179719356 27.661385240608816 27.530667542778154 22.737627036893674 90-91 22.125932100610072 27.445043854820362 27.63255619690718 22.796467847662385 92-93 22.160795765331713 27.418131552228214 27.605381761497583 22.815690920942487 94-95 22.016098450096028 27.60599340473831 27.55190666673657 22.826001478429088 96-97 22.147096832821152 27.365991033302254 27.53445520452057 22.952456929356018 98-99 22.02754491645827 27.408083127559074 27.509353772702887 23.05501818327977 100-101 22.0343085427083 27.50223253684341 27.47121294469425 22.992245975754038 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 525.0 1 307.0 2 96.0 3 98.5 4 71.0 5 57.5 6 100.0 7 194.0 8 300.5 9 327.0 10 244.5 11 192.5 12 223.0 13 251.5 14 272.5 15 294.5 16 315.5 17 350.0 18 380.0 19 420.5 20 511.0 21 600.0 22 712.5 23 901.0 24 1158.0 25 1555.0 26 2123.0 27 2866.5 28 3770.5 29 5029.0 30 6385.5 31 7889.0 32 9711.0 33 11474.5 34 13219.0 35 14884.5 36 16423.0 37 17771.5 38 18811.0 39 20149.5 40 21396.0 41 22014.5 42 22520.5 43 23205.5 44 23859.0 45 24380.5 46 24473.5 47 23941.5 48 23341.0 49 22409.0 50 20969.0 51 19703.5 52 18409.5 53 17034.0 54 15680.5 55 14092.0 56 12520.5 57 10955.5 58 9490.0 59 8287.0 60 7036.0 61 5725.5 62 4557.0 63 3488.5 64 2672.5 65 2094.5 66 1634.5 67 1272.0 68 1007.5 69 762.0 70 589.0 71 501.0 72 401.5 73 283.0 74 205.5 75 167.5 76 127.0 77 92.0 78 66.0 79 47.0 80 33.5 81 18.5 82 14.5 83 11.5 84 10.0 85 9.5 86 5.5 87 3.0 88 3.0 89 0.5 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016426989893906112 2 0.0 3 0.0017475521163729905 4 0.019397828491740196 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.737760581864952E-5 12-13 0.0 14-15 0.0 16-17 0.001135908875642444 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.7475521163729904E-4 28-29 0.004631013108388425 30-31 0.0 32-33 1.7475521163729904E-4 34-35 0.009698914245870098 36-37 0.0036698594443832803 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.3688802909324764E-4 60-61 0.0017475521163729905 62-63 0.008912515793502253 64-65 0.0030582162036527335 66-67 0.004543635502569776 68-69 0.0027087057803781354 70-71 8.737760581864952E-5 72-73 0.0064659428305800645 74-75 0.005330033954937622 76-77 0.001135908875642444 78-79 6.116432407305468E-4 80-81 0.008475627764409004 82-83 0.011446466362243089 84-85 0.0027960833861967846 86-87 0.0027960833861967846 88-89 6.116432407305468E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 8.737760581864952E-5 98-99 0.0 100-101 0.0017475521163729905 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 572229.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.94664203505192 #Duplication Level Percentage of deduplicated Percentage of total 1 70.62173107049725 46.57266018801786 2 19.593332535407384 25.84228973972494 3 6.184564610641765 12.235538055619283 4 2.054597157498255 5.419751330870905 5 0.7806461274842318 2.5740495362626072 6 0.3158791467167844 1.2498701408921655 7 0.14980503163570388 0.6915397157430574 8 0.0886660406658272 0.46777821155637306 9 0.057021797266085694 0.338435644725167 >10 0.14420382311544944 1.3991142889884023 >50 0.0050416852392436064 0.23868713111339698 >100 0.0023881626169567757 0.43730085749959025 >500 5.307028037681723E-4 0.2497112139463447 >1k 0.0015921084113045168 2.2832739450398774 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 4812 0.840922078398683 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 2489 0.43496572176523735 RNA PCR Primer, Index 26 (100% over 22bp) ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 1858 0.32469518322210167 RNA PCR Primer, Index 26 (100% over 24bp) TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 1453 0.2539193225089955 RNA PCR Primer, Index 26 (100% over 29bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1344 0.23487100444052994 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1092 0.19083269110793055 No Hit CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT 827 0.14452256002404631 RNA PCR Primer, Index 26 (100% over 27bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 600 0.10485312698237943 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.737760581864953E-5 0.0 22-23 0.0 0.0 0.0 3.4951042327459813E-4 0.0 24-25 0.0 0.0 0.0 8.737760581864953E-4 0.0 26-27 0.0 0.0 0.0 0.0010485312698237943 0.0 28-29 0.0 0.0 0.0 0.002621328174559486 0.0 30-31 0.0 0.0 0.0 0.005679544378212219 0.0 32-33 0.0 0.0 0.0 0.015291081018263666 0.0 34-35 0.0 0.0 0.0 0.026912302592144055 0.0 36-37 0.0 0.0 0.0 0.04386355812096206 0.0 38-39 0.0 0.0 0.0 0.07103799353056206 0.0 40-41 0.0 0.0 0.0 0.09637749921797042 0.0 42-43 0.0 0.0 0.0 0.12398882265666368 0.0 44-45 0.0 0.0 0.0 0.15972626343649132 0.0 46-47 0.0 0.0 0.0 0.19869667563160903 0.0 48-49 0.0 0.0 0.0 0.2353078924696232 0.0 50-51 0.0 0.0 0.0 0.27593847917529524 0.0 52-53 0.0 0.0 0.0 0.31272445122494663 0.0 54-55 0.0 0.0 0.0 0.3558889884993595 0.0 56-57 0.0 0.0 0.0 0.40980097128946624 0.0 58-59 0.0 0.0 0.0 0.4649362405610341 0.0 60-61 0.0 0.0 0.0 0.5172754264464052 0.0 62-63 0.0 0.0 0.0 0.5788766385485531 0.0 64-65 0.0 0.0 0.0 0.6511379185605763 0.0 66-67 0.0 0.0 0.0 0.7363310842337596 0.0 68-69 0.0 0.0 0.0 0.8312231641528129 0.0 70-71 0.0 0.0 0.0 0.9206978325111101 0.0 72-73 0.0 0.0 0.0 1.0245024282236657 8.737760581864953E-5 74-75 0.0 0.0 0.0 1.1417631752322932 1.7475521163729907E-4 76-77 0.0 0.0 0.0 1.262519026473667 1.7475521163729907E-4 78-79 0.0 0.0 0.0 1.4089638938257236 1.7475521163729907E-4 80-81 0.0 0.0 0.0 1.5630106128840027 1.7475521163729907E-4 82-83 0.0 0.0 0.0 1.7212514570215771 1.7475521163729907E-4 84-85 0.0 0.0 0.0 1.9038706531825547 1.7475521163729907E-4 86-87 0.0 0.0 0.0 2.1078973627691013 1.7475521163729907E-4 88-89 0.0 0.0 0.0 2.3494090652518484 1.7475521163729907E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 860 0.0 54.693565 1 GTATCAA 1445 0.0 38.140785 1 TCAACGC 1670 0.0 32.714695 4 CAACGCA 1720 0.0 31.755362 5 ATCAACG 1725 0.0 31.671618 3 AACGCAG 1750 0.0 31.210983 6 TATCAAC 1770 0.0 31.134813 2 ACGCAGA 1940 0.0 27.6646 7 CGCAGAG 2000 0.0 26.597185 8 GTGGTAT 375 0.0 24.072536 1 TATACAC 2155 0.0 23.14747 3 CTTATAC 2130 0.0 22.975082 1 TTATACA 2175 0.0 22.279346 2 TACACAT 2370 0.0 21.843681 5 ACACATC 2365 0.0 20.88574 6 GCAGAGT 2485 0.0 20.64168 9 ATTATAC 210 8.5792817E-7 20.360329 3 ACATCTC 2435 0.0 20.285328 8 GAGTACT 1515 0.0 20.220633 12-13 TGGTATC 400 0.0 20.190657 2 >>END_MODULE