FastQCFastQC Report
Fri 10 Feb 2017
SRR2031769_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031769_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254737
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33010.26308302058519034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24780.19749158588612592No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16240.12942951391407123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.11588085790089876No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13800.10998320763634133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.056.827891
GTATCAA28250.037.671121
ATCAACG33750.031.109823
TCAACGC34050.030.8357244
CAACGCA34700.030.39265
TATCAAC35400.029.92822
AACGCAG36550.029.1083986
ACGCAGA39550.026.9015037
CGCAGAG40350.025.8983128
TACACCG1452.3324557E-522.9337125
GCAGAGT47500.021.5999369
GAGTACT31900.021.2947412-13
CAGAGTA45500.020.09649710-11
GTACATG25250.019.7563571
AGAGTAC42350.019.4601910-11
TACATGG24150.019.279152
GTATATA6250.019.0037351
GTGGTAT6700.018.4364591
ACATGGG24900.018.3168533
GTACTTT37400.017.78282514-15
Produced by FastQC (version 0.11.5)