FastQCFastQC Report
Fri 10 Feb 2017
SRR2031769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254737
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38980.3106627125843902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30110.23997060738624904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18660.14871642423870501No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17650.1406669286073496No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13700.1091862278708606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17000.056.4505541
GTATCAA31000.041.224591
ATCAACG35700.034.5995833
TCAACGC35850.034.32234
TATCAAC37100.033.550052
CAACGCA36900.033.3376775
AACGCAG38100.032.4123346
ACGCAGA42750.028.553477
CGCAGAG43600.028.1057478
GCAGAGT50150.023.9613639
GTACATG24200.021.3981841
GAGTACT37450.021.2442912-13
CAGAGTA49200.021.0460510-11
TACATGG23550.019.5680082
GTACTTT41300.019.43640114-15
AGAGTAC47000.019.20149210-11
AGTACTT41100.018.08638412-13
ACATGGG25200.016.7785823
GTGGTAT7350.016.1591471
GTATAAT5700.015.8359641
Produced by FastQC (version 0.11.5)