##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031769_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1254737 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.77319470135973 34.0 31.0 34.0 31.0 34.0 2 32.93148683748068 34.0 31.0 34.0 31.0 34.0 3 32.99620876725561 34.0 31.0 34.0 31.0 34.0 4 36.38073317356545 37.0 37.0 37.0 35.0 37.0 5 36.351824326532174 37.0 37.0 37.0 35.0 37.0 6 36.376943534780594 37.0 37.0 37.0 35.0 37.0 7 36.32161241758233 37.0 37.0 37.0 35.0 37.0 8 36.31059018742573 37.0 37.0 37.0 35.0 37.0 9 38.1693940642541 39.0 39.0 39.0 37.0 39.0 10-11 38.13349450920791 39.0 39.0 39.0 37.0 39.0 12-13 38.084111650489305 39.0 39.0 39.0 37.0 39.0 14-15 39.58735774907411 41.0 40.0 41.0 37.0 41.0 16-17 39.56255653575211 41.0 40.0 41.0 37.0 41.0 18-19 39.56601104454559 41.0 39.5 41.0 37.0 41.0 20-21 39.554057145043146 41.0 40.0 41.0 37.0 41.0 22-23 39.458838784542095 41.0 39.0 41.0 36.0 41.0 24-25 39.390417673185695 41.0 39.0 41.0 36.0 41.0 26-27 39.25907102444576 41.0 39.0 41.0 36.0 41.0 28-29 39.155578818509376 41.0 39.0 41.0 36.0 41.0 30-31 38.992071645292995 40.0 39.0 41.0 35.0 41.0 32-33 38.79675820510593 40.0 38.0 41.0 35.0 41.0 34-35 38.65167959500676 40.0 38.0 41.0 35.0 41.0 36-37 38.54810251072536 40.0 38.0 41.0 35.0 41.0 38-39 38.62204549638689 40.0 38.0 41.0 35.0 41.0 40-41 38.54848545950267 40.0 38.0 41.0 35.0 41.0 42-43 38.45802188028248 40.0 38.0 41.0 35.0 41.0 44-45 38.287079682833934 40.0 38.0 41.0 34.0 41.0 46-47 38.096412634679616 40.0 38.0 41.0 34.0 41.0 48-49 37.87490844694944 40.0 38.0 41.0 33.0 41.0 50-51 37.74406987281 40.0 38.0 41.0 33.0 41.0 52-53 37.80786172719861 40.0 38.0 41.0 33.0 41.0 54-55 37.74398260352568 40.0 37.5 41.0 33.0 41.0 56-57 37.576993425713916 40.0 37.0 41.0 33.0 41.0 58-59 37.41340535905134 40.0 37.0 41.0 33.0 41.0 60-61 37.17087963453696 40.0 36.0 41.0 33.0 41.0 62-63 36.92032872227407 39.0 36.0 41.0 32.0 41.0 64-65 36.626173054592314 39.0 35.0 41.0 32.0 41.0 66-67 36.31093129476535 39.0 35.0 41.0 31.5 41.0 68-69 35.949978760489245 38.0 35.0 40.0 31.0 41.0 70-71 35.60816011642281 37.0 35.0 40.0 31.0 41.0 72-73 35.222630320138805 37.0 35.0 39.0 30.5 41.0 74-75 34.83717743240217 36.0 35.0 39.0 30.0 41.0 76-77 33.891695630239646 35.0 33.5 37.5 29.0 39.0 78-79 34.079717104062446 35.0 34.0 37.0 30.0 39.0 80-81 33.833795448767354 35.0 34.0 37.0 30.0 39.0 82-83 33.54336406752969 35.0 34.0 36.5 30.0 38.5 84-85 33.22873199722332 35.0 34.0 36.0 29.0 37.0 86-87 32.96378603643632 35.0 34.0 36.0 29.0 37.0 88-89 32.730452676537 35.0 34.0 35.0 29.0 36.5 90-91 32.522601549169266 35.0 34.0 35.0 29.0 36.0 92-93 32.34735924739607 35.0 34.0 35.0 29.0 36.0 94-95 32.16680467699606 35.0 34.0 35.0 28.0 36.0 96-97 32.0071871635251 35.0 34.0 35.0 27.0 35.5 98-99 31.802795725319328 35.0 34.0 35.0 27.0 35.0 100-101 31.094408629059316 34.5 32.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15779896509620528 1101 2 0.14498360885865935 1101 3 0.10643526971730921 1101 4 0.14895615164702036 1101 5 0.09777818104686276 1101 6 0.00867849334499482 1101 7 0.1331513489359466 1101 8 0.039811334637008144 1101 9 0.08038206674091697 1101 10-11 0.11274928524264283 1101 12-13 0.20948621246589028 1101 14-15 0.09743623254253464 1101 16-17 0.0029013874531145234 1101 18-19 0.014226514136638002 1101 20-21 0.02148116517422949 1101 22-23 -0.11606554356382048 1101 24-25 -0.1552536299432603 1101 26-27 0.004040637665376323 1101 28-29 -0.012817819235273475 1101 30-31 0.08542582446654734 1101 32-33 0.06552236414224666 1101 34-35 -0.11257401920691024 1101 36-37 0.10216480074008416 1101 38-39 0.10325862126760654 1101 40-41 -0.1392236399787805 1101 42-43 -0.08943051926943468 1101 44-45 -0.18078278002609238 1101 46-47 -0.0563147062363214 1101 48-49 -0.22585631053915733 1101 50-51 -0.04853531155536217 1101 52-53 -0.08966304671331926 1101 54-55 0.10910180042861128 1101 56-57 -0.12670984744428182 1101 58-59 -0.06793929827003353 1101 60-61 0.04204192271892282 1101 62-63 0.030873951375070874 1101 64-65 0.06693224123101515 1101 66-67 -0.252155717404392 1101 68-69 -0.31170960664266545 1101 70-71 -0.2348571375030133 1101 72-73 -0.2313253997756064 1101 74-75 -0.28020108973760927 1101 76-77 -0.3195043534335724 1101 78-79 -0.2916629095664618 1101 80-81 -0.35048166962652516 1101 82-83 -0.3610376073503829 1101 84-85 -0.279379389265884 1101 86-87 -0.285697287190807 1101 88-89 -0.23278754450264927 1101 90-91 -0.30940516906841253 1101 92-93 -0.5028981517339162 1101 94-95 -0.4314341767703276 1101 96-97 -0.4504666366989376 1101 98-99 -0.5524796048194887 1101 100-101 -0.7180192291301317 1102 1 0.10109169408581664 1102 2 -0.023339337316983233 1102 3 0.04620108652562038 1102 4 -0.024440448919548885 1102 5 0.007270154606821677 1102 6 -0.027328116664449453 1102 7 0.10063199105115217 1102 8 0.01875184455202117 1102 9 0.06694958326594502 1102 10-11 0.08434749109723327 1102 12-13 0.019019262513104707 1102 14-15 0.03413217210815844 1102 16-17 0.018658705677857768 1102 18-19 -0.025007669055057136 1102 20-21 0.050569928158175514 1102 22-23 0.1055567416108758 1102 24-25 0.07252493474040733 1102 26-27 0.15027057156528656 1102 28-29 0.1852841637675624 1102 30-31 -0.08584282520295261 1102 32-33 0.10925371447275012 1102 34-35 0.0011162546363365777 1102 36-37 0.04255856844545747 1102 38-39 -0.004824003850423253 1102 40-41 0.0711380219664477 1102 42-43 0.07813888582972339 1102 44-45 0.08909210193802863 1102 46-47 0.1876314694010759 1102 48-49 0.18312810872430418 1102 50-51 0.14131454680157418 1102 52-53 0.10132042826307242 1102 54-55 0.19022030845505356 1102 56-57 0.16708052082767466 1102 58-59 0.09963860541268588 1102 60-61 0.24138753178407057 1102 62-63 0.1575240930181323 1102 64-65 0.29233073037171664 1102 66-67 0.15107138363433137 1102 68-69 0.28799860865478877 1102 70-71 0.16481755560369038 1102 72-73 0.08906506292505156 1102 74-75 0.08778380166938149 1102 76-77 0.12446354080627486 1102 78-79 0.09647448420124505 1102 80-81 -0.018004804659575768 1102 82-83 0.018703185850753812 1102 84-85 0.08612249930823879 1102 86-87 0.15350431809719822 1102 88-89 -0.11097451334117636 1102 90-91 -0.08056239286445077 1102 92-93 -0.04167199498226637 1102 94-95 0.1513755493864224 1102 96-97 -0.13049968674615187 1102 98-99 -0.22948810340306025 1102 100-101 -0.22160940854467626 1103 1 0.16946315804611345 1103 2 0.13360327490689627 1103 3 0.11172654986573605 1103 4 0.0931732202926625 1103 5 0.061770759896582206 1103 6 0.13029073824295523 1103 7 0.13352611702499928 1103 8 0.12099500810639796 1103 9 0.20334125041438966 1103 10-11 0.123644461494969 1103 12-13 0.14001589706700912 1103 14-15 0.1969371601863159 1103 16-17 0.1978494394011605 1103 18-19 0.3146365326895051 1103 20-21 0.23744591471040621 1103 22-23 0.12328139521169135 1103 24-25 -0.013198898959956296 1103 26-27 0.046726166422331517 1103 28-29 0.16267383197066465 1103 30-31 0.1813562511826845 1103 32-33 0.23266800421645684 1103 34-35 0.17816438524577194 1103 36-37 0.20943196215009863 1103 38-39 0.11429850995035196 1103 40-41 0.22085613738670418 1103 42-43 0.2264804269272318 1103 44-45 0.39017733129117005 1103 46-47 0.37257026593436393 1103 48-49 0.5636696634868201 1103 50-51 0.39538120050401204 1103 52-53 0.3687441889082024 1103 54-55 0.607050780020451 1103 56-57 0.5781213213868881 1103 58-59 0.5805115366093716 1103 60-61 0.4444482306966506 1103 62-63 0.3371309087034575 1103 64-65 0.426926675368307 1103 66-67 0.4521439115381014 1103 68-69 0.5433200779584553 1103 70-71 0.39262987548399764 1103 72-73 0.5137831345472108 1103 74-75 0.5812386134163816 1103 76-77 0.537734978662904 1103 78-79 0.5655495208602659 1103 80-81 0.433939480651766 1103 82-83 0.6123492201078733 1103 84-85 0.3944545624966409 1103 86-87 0.30855967013757635 1103 88-89 0.4532050343470715 1103 90-91 0.5456681147905869 1103 92-93 0.5590842230341764 1103 94-95 0.5285945801869971 1103 96-97 0.37141648018417683 1103 98-99 0.5646038831211371 1103 100-101 0.3900661140980688 1104 1 0.173834679381919 1104 2 0.10237646600151606 1104 3 0.12979241257445295 1104 4 0.12988472307559107 1104 5 0.11684960961829205 1104 6 0.0416427790592806 1104 7 0.21811563465023198 1104 8 0.18145419177986355 1104 9 0.06252492388377817 1104 10-11 0.13042785667121848 1104 12-13 0.17684335532303663 1104 14-15 0.23982908968538652 1104 16-17 0.2668835303102597 1104 18-19 0.291619371279495 1104 20-21 0.3321061651742312 1104 22-23 0.23763088500760432 1104 24-25 0.24197851291388872 1104 26-27 0.23671920909394828 1104 28-29 0.214592895050437 1104 30-31 0.27160439589511753 1104 32-33 0.3055402212851064 1104 34-35 0.36835455222165336 1104 36-37 0.2940040864543647 1104 38-39 0.4006157641247512 1104 40-41 0.4135799314497888 1104 42-43 0.3767123378734283 1104 44-45 0.35787793425963343 1104 46-47 0.29414957947796694 1104 48-49 0.3637508323179901 1104 50-51 0.43153611701607275 1104 52-53 0.45097981042953705 1104 54-55 0.3721018004286165 1104 56-57 0.3851830096985793 1104 58-59 0.37152498744425344 1104 60-61 0.49852406557606344 1104 62-63 0.5972132370893632 1104 64-65 0.6209143840881595 1104 66-67 0.5955228540241819 1104 68-69 0.7096118219287675 1104 70-71 0.581875005354128 1104 72-73 0.5718710287958189 1104 74-75 0.5972810531195378 1104 76-77 0.6310849322807144 1104 78-79 0.6444978047192578 1104 80-81 0.554643330373473 1104 82-83 0.5482838212210481 1104 84-85 0.6199241821626842 1104 86-87 0.5012312842377682 1104 88-89 0.45873031264020625 1104 90-91 0.5345769737887309 1104 92-93 0.520030419694649 1104 94-95 0.6180658232296778 1104 96-97 0.7000690775867717 1104 98-99 0.7913953951805084 1104 100-101 0.7660343422984361 1105 1 0.10957625767262869 1105 2 0.08527181846564247 1105 3 0.023343566660543047 1105 4 0.03875091147234855 1105 5 0.024904818601449108 1105 6 0.02151255448036693 1105 7 0.009229951556037008 1105 8 0.06443142197325358 1105 9 -0.025185618848603042 1105 10-11 0.05815321537365037 1105 12-13 0.05691197665803571 1105 14-15 0.039755009835104715 1105 16-17 0.012495273915995142 1105 18-19 -0.025594446562053008 1105 20-21 -0.02806031954192889 1105 22-23 0.1373755044711089 1105 24-25 0.14798872813534558 1105 26-27 0.06553627085315128 1105 28-29 0.2181428794546818 1105 30-31 0.07023586813466665 1105 32-33 0.043061665452292175 1105 34-35 -0.0010543685518911161 1105 36-37 -0.04374349620314888 1105 38-39 -0.07946845296820015 1105 40-41 -0.014057701114154497 1105 42-43 0.008543279857207153 1105 44-45 0.0035949492315552334 1105 46-47 -0.006057064314923366 1105 48-49 -0.021273341106841315 1105 50-51 -0.009753652166715199 1105 52-53 0.16228673494607193 1105 54-55 -0.03526719520457533 1105 56-57 0.048154781376680944 1105 58-59 0.0954449812059508 1105 60-61 0.04429956464031193 1105 62-63 0.06012067626590323 1105 64-65 0.17220298358909503 1105 66-67 0.27728751403665797 1105 68-69 0.26130786060624445 1105 70-71 0.23073456555375316 1105 72-73 0.21277285349950148 1105 74-75 0.2681657224894636 1105 76-77 0.08233844132625023 1105 78-79 0.1749855620492653 1105 80-81 0.24420391989313117 1105 82-83 0.21018728347931415 1105 84-85 0.4610638421751716 1105 86-87 0.3070952892284069 1105 88-89 0.2733259926152556 1105 90-91 0.2909419925036403 1105 92-93 0.3647590535062619 1105 94-95 0.30332783196329416 1105 96-97 0.2661054157028069 1105 98-99 0.3736688667962298 1105 100-101 0.3480855743633171 1106 1 0.10395459708244914 1106 2 0.05618051341583907 1106 3 0.02000706679383768 1106 4 0.04049613445011602 1106 5 0.09783321114144172 1106 6 0.07603274957887152 1106 7 0.04152108238392316 1106 8 0.08313549628790895 1106 9 0.029801671212112524 1106 10-11 0.0332845388969929 1106 12-13 0.02500082983476659 1106 14-15 0.09268730734906683 1106 16-17 0.05153337369559097 1106 18-19 0.07777509539201333 1106 20-21 0.10177781177783629 1106 22-23 0.13534116322895784 1106 24-25 0.11808901838004715 1106 26-27 0.11098603749340441 1106 28-29 0.14412973020582598 1106 30-31 0.19119581586809176 1106 32-33 0.094374900685672 1106 34-35 0.21570285095645403 1106 36-37 0.020384061273183818 1106 38-39 0.04643016039056391 1106 40-41 0.2452656119558725 1106 42-43 0.12225649706763164 1106 44-45 0.04360028102292546 1106 46-47 0.12220893950744482 1106 48-49 0.24527567570331854 1106 50-51 0.07924370822107818 1106 52-53 0.2036606850149667 1106 54-55 0.10820455193334766 1106 56-57 0.22399350595210876 1106 58-59 0.15734445065515246 1106 60-61 0.14307287714711947 1106 62-63 0.21099647932004473 1106 64-65 0.26609733151477144 1106 66-67 0.19840103238065154 1106 68-69 0.20195075449233002 1106 70-71 0.15006418665863208 1106 72-73 0.24152885645826672 1106 74-75 0.37016176494854847 1106 76-77 0.21887397846668222 1106 78-79 0.28326185397610715 1106 80-81 0.28184937078619754 1106 82-83 0.3890857804398138 1106 84-85 0.2881962771141673 1106 86-87 0.3737408039527921 1106 88-89 0.4198349834423212 1106 90-91 0.40300798656357273 1106 92-93 0.4181633271998777 1106 94-95 0.3503745076664728 1106 96-97 0.4576717983827443 1106 98-99 0.30851437626391487 1106 100-101 0.23975033234880172 1107 1 0.2882254356844385 1107 2 0.20810125591748374 1107 3 0.1711264461878983 1107 4 0.13088262223525504 1107 5 0.10739582810568749 1107 6 0.14011966981558288 1107 7 0.13736458423006326 1107 8 0.21420104051936306 1107 9 0.16567618438797638 1107 10-11 0.18032281465441713 1107 12-13 0.2318722418776602 1107 14-15 0.31761270313076295 1107 16-17 0.3035300639237093 1107 18-19 0.2524912200189888 1107 20-21 0.3608745475271675 1107 22-23 0.40896754467146934 1107 24-25 0.32448901711556744 1107 26-27 0.38679799060655284 1107 28-29 0.39368953370590276 1107 30-31 0.31497361858419737 1107 32-33 0.391503981789306 1107 34-35 0.3719259807930868 1107 36-37 0.4382530360341974 1107 38-39 0.4366189153852531 1107 40-41 0.42156312472710056 1107 42-43 0.3953047748482206 1107 44-45 0.4800811905621458 1107 46-47 0.3996117643519099 1107 48-49 0.3364267776961327 1107 50-51 0.40085071785640736 1107 52-53 0.3071641591690266 1107 54-55 0.3343260651344977 1107 56-57 0.45478647608513256 1107 58-59 0.38047981937702247 1107 60-61 0.2952808933071509 1107 62-63 0.2771239513750743 1107 64-65 0.4910168000545525 1107 66-67 0.5070906061250255 1107 68-69 0.5556727462985123 1107 70-71 0.5202678624969863 1107 72-73 0.44991724728321714 1107 74-75 0.4634312632035673 1107 76-77 0.40410226421348483 1107 78-79 0.5311312080806019 1107 80-81 0.5427867127264179 1107 82-83 0.46070503970844356 1107 84-85 0.5118228166164656 1107 86-87 0.5909240363386061 1107 88-89 0.4165690731444087 1107 90-91 0.44254703681393437 1107 92-93 0.5217636129719665 1107 94-95 0.5305658232296722 1107 96-97 0.6460774809481187 1107 98-99 0.6515056892981548 1107 100-101 0.6870690061639877 1108 1 0.054213599242551425 1108 2 0.007276291785487388 1108 3 -0.05808505548593956 1108 4 0.07075289961450437 1108 5 0.04041232738832434 1108 6 0.01187361529621711 1108 7 -0.04071856976324284 1108 8 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-0.10034930330599678 2316 86-87 -0.11518296054324395 2316 88-89 -0.20900530954562413 2316 90-91 -0.17949256161855942 2316 92-93 -0.059994140272600305 2316 94-95 -0.1606591051370927 2316 96-97 -0.2527732269568155 2316 98-99 -0.1876429286017256 2316 100-101 0.14667418061198845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 2.0 5 10.0 6 28.0 7 67.0 8 161.0 9 403.0 10 722.0 11 1112.0 12 1794.0 13 2463.0 14 2820.0 15 3339.0 16 4021.0 17 5774.0 18 5629.0 19 3671.0 20 2976.0 21 2875.0 22 2943.0 23 3403.0 24 3690.0 25 4491.0 26 5386.0 27 6508.0 28 8263.0 29 10764.0 30 13668.0 31 17717.0 32 23116.0 33 30803.0 34 43820.0 35 70667.0 36 122405.0 37 258776.0 38 437126.0 39 153111.0 40 212.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.12790794995755 20.874746419988284 14.353126855767275 23.644218774286887 2 15.353655786033249 22.523365454274483 39.56566196740831 22.55731679228396 3 17.181483952158136 27.232276282221406 31.571581480126593 24.014658285493862 4 12.50532092130457 19.40059721861294 39.011560149926424 29.082521710156062 5 13.13303106547428 37.77827544736467 35.87660202895109 13.212091458209969 6 28.959216154461053 38.33863192047417 19.391713163794485 13.31043876127029 7 24.76351617908773 33.17946310661119 24.958696523653963 17.098324190647123 8 23.734455905899004 34.769836228627995 22.991351972564768 18.504355892908233 9 25.140806400066307 16.04894093343864 22.387002216400727 36.42325045009432 10-11 22.89603319261327 26.601750008169045 30.92966892663562 19.572547872582064 12-13 23.961445291501445 25.092559274397452 29.99880851477581 20.947186919325294 14-15 21.686898702474508 26.70847896507189 27.713313055471094 23.8913092769825 16-17 21.06152851754058 28.902128683792945 28.545116476278753 21.49122632238772 18-19 20.879292536437944 28.25825512299002 30.218189324288137 20.644263016283904 20-21 21.68171497293855 27.675401299236412 29.970981966738847 20.671901761086186 22-23 21.394961653318585 27.690982253651562 29.572292839057106 21.341763253972744 24-25 21.350530031393035 27.712939046190556 29.753326792786055 21.183204129630354 26-27 21.371573029991602 27.789265378452573 29.69030500077905 21.148856590776774 28-29 21.24039842907839 27.642681087371056 29.847590317033664 21.269330166516895 30-31 21.572346692850132 27.499888422743908 29.660183496767445 21.267581387638515 32-33 21.45030623996302 27.577376896316753 29.588438993094158 21.38387787062607 34-35 21.708087901226698 27.722164213009826 29.462493723048965 21.10725416271451 36-37 21.68389691975519 27.593404815437967 29.359681765687156 21.36301649911969 38-39 21.510165078418826 27.701980574415195 29.485143101701787 21.302711245464188 40-41 21.76483996247819 27.515447460304433 29.40014520971327 21.319567367504106 42-43 21.68414576122327 27.567450390002048 29.185797501787224 21.562606346987458 44-45 21.749856742887154 27.65105356740098 29.116539960166953 21.482549729544917 46-47 21.885701943913347 27.725053138625867 28.771726664631714 21.61751825282908 48-49 21.819114284507428 27.633559861548672 28.873062641812584 21.674263212131308 50-51 21.77261051519163 27.77761395415932 28.729088247178492 21.72068728347056 52-53 21.801142390795842 27.875323673407255 28.713706537704713 21.60982739809219 54-55 21.840911681093328 27.85659464891846 28.525858406980902 21.776635263007307 56-57 21.80070405192483 27.92641007637457 28.46453081402716 21.808355057673438 58-59 21.812515267210557 27.824199838770287 28.679362875643115 21.68392201837604 60-61 21.972062900515375 28.00568098722296 28.49746734607916 21.524788766182503 62-63 21.81813182149138 28.01665991167904 28.4724123515447 21.692795915284883 64-65 21.78448833074176 27.951625359546096 28.421416314725274 21.842469994986878 66-67 21.712991578266962 27.998068018655097 28.356170006388133 21.932770396689808 68-69 21.74831681866274 27.95398075624107 28.389465944843057 21.90823648025313 70-71 21.791467770324687 27.76877854433979 28.465339136942298 21.974414548393227 72-73 21.817162332233153 27.860235939625856 28.318214822861957 22.00438690527903 74-75 21.730848244121002 27.966419494100574 28.232587963319045 22.07014429845938 76-77 21.78428709650167 27.848019548678028 28.24149567152355 22.12619768329675 78-79 21.671352260342346 27.837806066233238 28.212706692462337 22.27813498096208 80-81 21.6842612850647 27.937623789996373 28.134492226884895 22.24362269805403 82-83 21.762258920451423 27.919436768352412 28.16576692329213 22.152537387904037 84-85 21.846019338240687 27.84315335103721 28.041206137484497 22.26962117323761 86-87 21.62667821774879 27.83246255229767 28.229006313378363 22.311852916575177 88-89 21.708061960641796 27.805539350235748 28.14258343614413 22.343815252978324 90-91 21.747226709661067 27.719434431279222 28.18777959046398 22.34555926859573 92-93 21.698332001048826 27.69807537356434 28.25671834017806 22.346874285208774 94-95 21.604487633663467 27.675640393166056 28.19766213955594 22.522209833614536 96-97 21.58101657957006 27.612997783599276 28.271940653698742 22.534044983131924 98-99 21.546427657748197 27.588490655810737 28.162873972792706 22.70220771364836 100-101 21.551916514043704 27.64870577321264 28.092547695672206 22.706830017071454 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2609.0 1 1537.5 2 503.5 3 512.0 4 358.5 5 313.0 6 489.5 7 815.5 8 1271.5 9 1435.0 10 1060.0 11 753.5 12 777.5 13 814.5 14 848.5 15 879.0 16 949.0 17 1060.0 18 1175.0 19 1335.0 20 1481.0 21 1685.0 22 2133.5 23 2742.0 24 3352.0 25 4277.5 26 5587.0 27 7110.5 28 9071.5 29 11930.5 30 15572.0 31 20040.5 32 25524.0 33 31008.0 34 36134.5 35 40180.5 36 42982.5 37 45065.0 38 45992.0 39 46116.5 40 45938.0 41 45768.0 42 45605.0 43 45962.0 44 46533.0 45 46909.0 46 47420.0 47 47054.5 48 46118.5 49 44844.0 50 42283.0 51 39869.5 52 37906.0 53 35455.0 54 32628.0 55 29565.0 56 26432.0 57 23278.5 58 20560.0 59 18023.0 60 15173.0 61 12418.5 62 10116.0 63 7799.5 64 5787.5 65 4431.5 66 3411.5 67 2614.5 68 2043.0 69 1595.0 70 1184.0 71 897.5 72 704.5 73 569.5 74 442.5 75 329.0 76 252.0 77 198.5 78 140.0 79 92.0 80 65.0 81 44.5 82 32.0 83 21.0 84 15.0 85 11.0 86 8.0 87 4.5 88 1.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01609899126271083 2 0.0 3 0.0015939595309614684 4 0.020322984019758725 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.984898827403671E-5 14-15 1.9924494137018355E-4 16-17 0.0014345635778653216 18-19 7.969797654807342E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.9924494137018355E-4 28-29 0.004742029604610369 30-31 7.969797654807342E-5 32-33 1.5939595309614685E-4 34-35 0.011715602552566793 36-37 0.005100670499076699 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.383388710144038E-4 60-61 0.0020322984019758724 62-63 0.008647230455465966 64-65 0.0023909392964422027 66-67 0.005299915440446883 68-69 0.0035465599563892676 70-71 3.984898827403671E-5 72-73 0.007491609795518901 74-75 0.00641568711211991 76-77 0.0018330534606056889 78-79 3.586408944663304E-4 80-81 0.0073322138424227554 82-83 0.009563757185768811 84-85 0.0016736575075095416 86-87 0.0016338085192355051 88-89 3.984898827403671E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.766777420288076E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1254737.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.77439562703139 #Duplication Level Percentage of deduplicated Percentage of total 1 66.67182590993814 40.51939925027142 2 20.292088962906718 24.664788854612198 3 7.298879637172552 13.307549961108242 4 2.822227376387563 6.860766524880664 5 1.252716892844068 3.8066556027185436 6 0.604645459404912 2.204817741837736 7 0.32620217738751295 1.3877318128063447 8 0.1927057940144214 0.936926253204293 9 0.1280394751004044 0.7003369544066469 >10 0.39674219093776 3.695758857183799 >50 0.01012624241470053 0.4178563331818088 >100 0.0027516432548777415 0.364798001165848 >500 2.620595590806885E-4 0.08735691459796222 >1k 7.861786772420656E-4 1.0452569380244776 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3898 0.3106627125843902 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3011 0.23997060738624904 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1866 0.14871642423870501 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1765 0.1406669286073496 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1370 0.1091862278708606 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 7.969797654807342E-5 5 0.0 0.0 0.0 0.0 7.969797654807342E-5 6 0.0 0.0 0.0 0.0 7.969797654807342E-5 7 0.0 0.0 0.0 0.0 7.969797654807342E-5 8 0.0 0.0 0.0 7.969797654807342E-5 7.969797654807342E-5 9 0.0 0.0 0.0 2.3909392964422026E-4 7.969797654807342E-5 10-11 0.0 0.0 0.0 2.3909392964422026E-4 7.969797654807342E-5 12-13 0.0 0.0 0.0 2.3909392964422026E-4 7.969797654807342E-5 14-15 0.0 0.0 0.0 2.3909392964422026E-4 7.969797654807342E-5 16-17 7.969797654807342E-5 0.0 0.0 3.187919061922937E-4 7.969797654807342E-5 18-19 1.5939595309614685E-4 0.0 0.0 3.187919061922937E-4 7.969797654807342E-5 20-21 2.3909392964422026E-4 0.0 0.0 3.586408944663304E-4 7.969797654807342E-5 22-23 3.984898827403671E-4 0.0 0.0 5.180368475624773E-4 7.969797654807342E-5 24-25 3.984898827403671E-4 0.0 0.0 7.969797654807342E-4 7.969797654807342E-5 26-27 3.984898827403671E-4 0.0 0.0 9.563757185768811E-4 7.969797654807342E-5 28-29 3.984898827403671E-4 0.0 0.0 0.0023909392964422027 7.969797654807342E-5 30-31 4.781878592884405E-4 0.0 0.0 0.004622482639788259 7.969797654807342E-5 32-33 5.57885835836514E-4 0.0 0.0 0.010719377845715874 7.969797654807342E-5 34-35 5.57885835836514E-4 0.0 0.0 0.0208410208673212 7.969797654807342E-5 36-37 5.57885835836514E-4 0.0 0.0 0.03363254610328698 7.969797654807342E-5 38-39 5.57885835836514E-4 0.0 0.0 0.05395553012304571 7.969797654807342E-5 40-41 5.57885835836514E-4 0.0 0.0 0.07754613118127543 7.969797654807342E-5 42-43 5.57885835836514E-4 0.0 0.0 0.10380661445386563 7.969797654807342E-5 44-45 5.57885835836514E-4 0.0 0.0 0.1352474662020806 7.969797654807342E-5 46-47 5.57885835836514E-4 0.0 0.0 0.17266566619140106 7.969797654807342E-5 48-49 5.57885835836514E-4 0.0 0.0 0.2076530778960053 7.969797654807342E-5 50-51 5.57885835836514E-4 0.0 0.0 0.24702387831075356 7.969797654807342E-5 52-53 6.375838123845874E-4 0.0 0.0 0.2836450985345933 7.969797654807342E-5 54-55 6.375838123845874E-4 0.0 0.0 0.3303879617800384 7.969797654807342E-5 56-57 6.375838123845874E-4 0.0 0.0 0.38561865952785324 7.969797654807342E-5 58-59 6.375838123845874E-4 0.0 0.0 0.44204482692388924 7.969797654807342E-5 60-61 6.375838123845874E-4 0.0 0.0 0.4992679740854059 7.969797654807342E-5 62-63 6.375838123845874E-4 0.0 0.0 0.5681668748112154 7.969797654807342E-5 64-65 6.375838123845874E-4 0.0 0.0 0.6412897682940728 7.969797654807342E-5 66-67 6.375838123845874E-4 0.0 0.0 0.7278019218370064 7.969797654807342E-5 68-69 7.172817889326608E-4 0.0 0.0 0.8230410038119542 7.969797654807342E-5 70-71 7.172817889326608E-4 0.0 0.0 0.9215477028253729 7.969797654807342E-5 72-73 7.172817889326608E-4 0.0 0.0 1.0253543172792385 7.969797654807342E-5 74-75 7.172817889326608E-4 0.0 0.0 1.140956232262219 7.969797654807342E-5 76-77 7.172817889326608E-4 0.0 0.0 1.26998725629355 7.969797654807342E-5 78-79 7.172817889326608E-4 0.0 0.0 1.4114511646663803 7.969797654807342E-5 80-81 7.172817889326608E-4 0.0 0.0 1.5638735448145706 7.969797654807342E-5 82-83 7.172817889326608E-4 0.0 0.0 1.728370168409794 7.969797654807342E-5 84-85 7.172817889326608E-4 0.0 0.0 1.924666284647699 7.969797654807342E-5 86-87 7.172817889326608E-4 0.0 0.0 2.135945620476642 1.1954696482211014E-4 88-89 7.172817889326608E-4 0.0 0.0 2.385440136060385 1.5939595309614685E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1700 0.0 56.450554 1 GTATCAA 3100 0.0 41.22459 1 ATCAACG 3570 0.0 34.599583 3 TCAACGC 3585 0.0 34.3223 4 TATCAAC 3710 0.0 33.55005 2 CAACGCA 3690 0.0 33.337677 5 AACGCAG 3810 0.0 32.412334 6 ACGCAGA 4275 0.0 28.55347 7 CGCAGAG 4360 0.0 28.105747 8 GCAGAGT 5015 0.0 23.961363 9 GTACATG 2420 0.0 21.398184 1 GAGTACT 3745 0.0 21.24429 12-13 CAGAGTA 4920 0.0 21.04605 10-11 TACATGG 2355 0.0 19.568008 2 GTACTTT 4130 0.0 19.436401 14-15 AGAGTAC 4700 0.0 19.201492 10-11 AGTACTT 4110 0.0 18.086384 12-13 ACATGGG 2520 0.0 16.778582 3 GTGGTAT 735 0.0 16.159147 1 GTATAAT 570 0.0 15.835964 1 >>END_MODULE