FastQCFastQC Report
Fri 10 Feb 2017
SRR2031766_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031766_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067487
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18510.17339789618046872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14020.13133649402756192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.048.730271
GTATCAA24450.038.8717041
TCAACGC27950.033.6624264
ATCAACG28350.033.187473
CAACGCA29200.032.5422825
TATCAAC29250.032.0053632
AACGCAG30300.031.5073346
ACGCAGA33850.028.0627047
CGCAGAG34650.027.691548
GTATTCG700.00837352127.1546611
GTGGTAT8050.022.432111
GCAGAGT40200.022.3323679
GAGTACG1302.932678E-421.9326111
GAGTACT25600.021.06131412-13
GGACCGA1908.256138E-619.998376
CAGAGTA37700.019.52987710-11
AGAGTAC35200.018.89272310-11
GTACTTT27450.018.69050814-15
ACCGTGC2051.5890475E-518.5368148
TGGTATC8450.017.9959892
Produced by FastQC (version 0.11.5)