##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031766_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1067487 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.712222256570804 33.0 31.0 34.0 30.0 34.0 2 32.02556190379836 34.0 31.0 34.0 30.0 34.0 3 32.14264623363095 34.0 31.0 34.0 30.0 34.0 4 35.54425393470834 37.0 35.0 37.0 35.0 37.0 5 35.53866323430637 37.0 37.0 37.0 35.0 37.0 6 35.55944849913863 37.0 37.0 37.0 35.0 37.0 7 35.53190624335472 37.0 37.0 37.0 35.0 37.0 8 35.51284933680691 37.0 37.0 37.0 35.0 37.0 9 37.28122122330296 39.0 38.0 39.0 35.0 39.0 10-11 37.20294486021844 39.0 38.0 39.0 35.0 39.0 12-13 37.14611419155456 39.0 38.0 39.0 35.0 39.0 14-15 38.535714252257876 41.0 39.0 41.0 35.0 41.0 16-17 38.46496116580342 41.0 39.0 41.0 35.0 41.0 18-19 38.45859668548657 41.0 39.0 41.0 34.5 41.0 20-21 38.44619653447771 41.0 39.0 41.0 34.0 41.0 22-23 38.34533582141984 41.0 39.0 41.0 34.0 41.0 24-25 38.266827605394724 41.0 38.5 41.0 34.0 41.0 26-27 38.152579375673895 40.0 38.0 41.0 34.0 41.0 28-29 38.03115026225144 40.0 38.0 41.0 34.0 41.0 30-31 37.866567461711476 40.0 38.0 41.0 33.5 41.0 32-33 37.67230795316477 40.0 38.0 41.0 33.0 41.0 34-35 37.50430918596667 40.0 38.0 41.0 33.0 41.0 36-37 37.33273145246734 40.0 38.0 41.0 32.0 41.0 38-39 37.14868893016964 40.0 37.5 41.0 31.5 41.0 40-41 37.05495523598882 40.0 37.0 41.0 31.0 41.0 42-43 37.02448975959426 40.0 37.0 41.0 31.0 41.0 44-45 36.90190653375638 40.0 37.0 41.0 31.0 41.0 46-47 36.84476953817705 40.0 37.0 41.0 31.0 41.0 48-49 36.91068228465546 40.0 37.0 41.0 31.0 41.0 50-51 36.37149445379663 39.5 36.5 40.5 30.5 41.0 52-53 36.38905579178014 39.5 37.0 40.5 30.5 41.0 54-55 36.61337561956258 40.0 37.0 41.0 31.0 41.0 56-57 36.487180171749166 40.0 36.0 41.0 30.0 41.0 58-59 36.30350767737687 40.0 36.0 41.0 30.0 41.0 60-61 36.064544111544215 39.0 35.5 41.0 29.5 41.0 62-63 35.782664800601786 39.0 35.0 41.0 29.0 41.0 64-65 35.50893968732172 39.0 35.0 41.0 28.0 41.0 66-67 35.21937644205503 38.0 35.0 41.0 28.0 41.0 68-69 34.88126740653516 37.0 35.0 40.0 28.0 41.0 70-71 34.519088288663 37.0 35.0 39.5 28.0 41.0 72-73 34.15482296271524 36.0 34.5 39.0 27.0 41.0 74-75 33.74802831322536 36.0 34.0 39.0 26.0 41.0 76-77 33.36562365630682 35.0 34.0 38.0 26.0 40.0 78-79 32.97738145757279 35.0 34.0 37.0 26.0 39.0 80-81 32.6271921812631 35.0 34.0 37.0 25.5 39.0 82-83 32.30191468373854 35.0 34.0 36.0 25.0 38.0 84-85 31.983692541454836 35.0 33.5 36.0 24.5 37.0 86-87 31.71108032228964 35.0 33.0 35.5 23.5 37.0 88-89 31.44383959710985 35.0 33.0 35.0 22.0 36.5 90-91 31.202724717022313 35.0 33.0 35.0 20.0 36.0 92-93 30.987974560814322 35.0 33.0 35.0 19.5 36.0 94-95 30.737798680452315 35.0 33.0 35.0 16.5 36.0 96-97 30.481735140568453 35.0 32.0 35.0 4.5 35.0 98-99 30.228976558965122 35.0 32.0 35.0 2.0 35.0 100-101 29.648783544904994 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06240835590237381 1101 2 0.11141181900261188 1101 3 0.3646572109574109 1101 4 0.3454538880504785 1101 5 0.23709325278084492 1101 6 0.3549764400086559 1101 7 0.5334410517807697 1101 8 0.35820016979378977 1101 9 0.4057011309394909 1101 10-11 0.43804006440568344 1101 12-13 0.40222177090018363 1101 14-15 0.26593763342692256 1101 16-17 0.34967548188723896 1101 18-19 0.42984963053362435 1101 20-21 0.3880349856216654 1101 22-23 0.4446845238744501 1101 24-25 0.32083126421915864 1101 26-27 0.14250806866218824 1101 28-29 0.198100060287409 1101 30-31 0.3711873001361923 1101 32-33 0.08518389065471155 1101 34-35 0.11667466699503848 1101 36-37 -0.13990128994092998 1101 38-39 0.22202653905394243 1101 40-41 -0.13850645267152117 1101 42-43 -0.05047995974260289 1101 44-45 -0.30784253206591217 1101 46-47 -0.18840042224471887 1101 48-49 -0.08989403985419386 1101 50-51 0.1530397657292113 1101 52-53 0.02339908183128614 1101 54-55 0.1378529968538018 1101 56-57 0.05542556274232169 1101 58-59 -0.26814533083864944 1101 60-61 -0.35945610467894795 1101 62-63 -0.1513336404775032 1101 64-65 0.13226504445996312 1101 66-67 -0.23582894542764876 1101 68-69 -0.4238655156989566 1101 70-71 -0.48337436885378793 1101 72-73 0.08266408663465796 1101 74-75 0.04330837626917372 1101 76-77 -0.19745948628315801 1101 78-79 -0.5311226976595904 1101 80-81 -0.5503384932586997 1101 82-83 -0.5326371803868071 1101 84-85 -0.4801729884635897 1101 86-87 -0.6652598385818784 1101 88-89 -0.9352263263832015 1101 90-91 -1.243529673571441 1101 92-93 -0.9538972361944538 1101 94-95 -1.1475434865378276 1101 96-97 -0.9295941652485986 1101 98-99 -0.6903567677893143 1101 100-101 -0.6471199019988454 1102 1 0.18819937473328707 1102 2 0.2933661196719939 1102 3 0.40067940633714727 1102 4 0.48131880324500287 1102 5 0.452051227515355 1102 6 0.40346151726468094 1102 7 0.4065058763819067 1102 8 0.2740766608099463 1102 9 0.49391015001443606 1102 10-11 0.3975951502177537 1102 12-13 0.45410598751904274 1102 14-15 0.5051375176687003 1102 16-17 0.5406985580360057 1102 18-19 0.435911837992478 1102 20-21 0.2945243660635555 1102 22-23 0.4414659421642497 1102 24-25 0.31808074834014377 1102 26-27 0.42809428336851596 1102 28-29 0.2602672554154992 1102 30-31 0.18977467242468293 1102 32-33 0.28745564564992065 1102 34-35 0.24289956507747945 1102 36-37 0.1523064205628728 1102 38-39 0.045200830663986835 1102 40-41 0.07489759383308581 1102 42-43 0.3928802999584349 1102 44-45 0.12896669336063127 1102 46-47 0.15908875687853907 1102 48-49 0.11480989592515556 1102 50-51 0.11438218356603613 1102 52-53 0.09118684165815694 1102 54-55 0.10633278949560321 1102 56-57 0.2059464747157449 1102 58-59 0.09753864923080613 1102 60-61 0.0691481280453985 1102 62-63 0.20851650296203417 1102 64-65 0.24621265128465808 1102 66-67 0.22854457613861 1102 68-69 0.13970776428493537 1102 70-71 0.15676101792964658 1102 72-73 0.20273870035453 1102 74-75 -0.12864667934510265 1102 76-77 0.0634507256047101 1102 78-79 0.135872999154536 1102 80-81 0.23799949556811129 1102 82-83 0.289460685635639 1102 84-85 0.24788328009546845 1102 86-87 0.17925234119566724 1102 88-89 0.2973080236092507 1102 90-91 0.19939083073174402 1102 92-93 0.4186844236777105 1102 94-95 -0.030018699180637043 1102 96-97 -0.24702269713244007 1102 98-99 0.11520780516352502 1102 100-101 -0.1848048634966588 1103 1 -0.05943683599537053 1103 2 0.16527697448066903 1103 3 0.15975311331138187 1103 4 0.08802872094788938 1103 5 0.1395925262478599 1103 6 0.16612145599238204 1103 7 0.1596325658755049 1103 8 0.20041173619890884 1103 9 0.12363923032920354 1103 10-11 0.12923448463236298 1103 12-13 0.13191880664561495 1103 14-15 0.2820702983499075 1103 16-17 0.22687324416564536 1103 18-19 0.1831335596240038 1103 20-21 0.050237107097977685 1103 22-23 0.2008309929241463 1103 24-25 0.13559441446617626 1103 26-27 -0.005236046420634466 1103 28-29 -0.06443989902674474 1103 30-31 -0.07148561471414183 1103 32-33 -0.16191361007182792 1103 34-35 -0.26361812579775545 1103 36-37 -0.33435784326844953 1103 38-39 -0.19469825025149134 1103 40-41 -0.001969108876110681 1103 42-43 0.02569702369244453 1103 44-45 -0.30927743471048785 1103 46-47 -0.18461881805989577 1103 48-49 -0.30681862573039353 1103 50-51 -0.21856369256779828 1103 52-53 -0.20668664906089873 1103 54-55 -0.5199572327306186 1103 56-57 -0.5829644401638063 1103 58-59 -0.4668303061365293 1103 60-61 -0.47385962109859037 1103 62-63 -0.19015593050946933 1103 64-65 -0.05225253763826743 1103 66-67 -0.37810226713644823 1103 68-69 -0.5196843183726045 1103 70-71 -0.49302272688924376 1103 72-73 -0.49120615457428585 1103 74-75 -0.3759374824928159 1103 76-77 -0.3403757896833284 1103 78-79 -0.32868517949626863 1103 80-81 -0.3612255900328947 1103 82-83 -0.30127638410665725 1103 84-85 -0.6558050721601916 1103 86-87 -0.6404959618018715 1103 88-89 -0.5134957248138896 1103 90-91 -0.3397843960358422 1103 92-93 -0.8498504474702457 1103 94-95 -0.4597928326581382 1103 96-97 -0.48291805365313323 1103 98-99 -0.41629036267452335 1103 100-101 -0.38891371193781765 1104 1 0.16130275016167417 1104 2 0.31044278273945736 1104 3 0.3064866454339068 1104 4 0.24721594422429405 1104 5 0.2892830476177011 1104 6 0.4018289648023554 1104 7 0.2723595921698987 1104 8 0.2316103446745572 1104 9 0.24687949417668165 1104 10-11 0.4307344394530048 1104 12-13 0.36267013943679416 1104 14-15 0.47487661442472984 1104 16-17 0.5044326550267613 1104 18-19 0.37984111361333817 1104 20-21 0.47142509842353775 1104 22-23 0.4648885514314216 1104 24-25 0.3777716079242026 1104 26-27 0.3290144279626688 1104 28-29 0.27844773989979643 1104 30-31 0.5355307402149236 1104 32-33 0.3204969347912865 1104 34-35 0.5642530226616245 1104 36-37 0.5297652253138239 1104 38-39 0.36359668062408446 1104 40-41 0.45029663613156856 1104 42-43 0.5076196314452233 1104 44-45 0.362240365958165 1104 46-47 0.22166563120368465 1104 48-49 0.6118528751280152 1104 50-51 0.5586060462954947 1104 52-53 0.6044139201990646 1104 54-55 0.7096600980726677 1104 56-57 0.5584774591765722 1104 58-59 0.46983161669712104 1104 60-61 0.3760556675975266 1104 62-63 0.4704725144168904 1104 64-65 0.4334233456182588 1104 66-67 0.46064694222471303 1104 68-69 0.4565332654351195 1104 70-71 0.6062047060194047 1104 72-73 0.2822249920779214 1104 74-75 0.3365128202089167 1104 76-77 0.3802621429090607 1104 78-79 0.2422136639120609 1104 80-81 0.1334850361530684 1104 82-83 0.08317400895379734 1104 84-85 0.26300874368873295 1104 86-87 0.28171760104262233 1104 88-89 0.3579260697103699 1104 90-91 0.34815497326026446 1104 92-93 0.22114440208247998 1104 94-95 0.2864030650557865 1104 96-97 0.18710656909919265 1104 98-99 0.09411783286175535 1104 100-101 0.10706758380628756 1105 1 -0.047076787220291294 1105 2 0.20472936335108471 1105 3 0.1594538116818498 1105 4 0.23207040147111258 1105 5 0.3383842344841952 1105 6 0.2906641896139277 1105 7 0.20343037099689099 1105 8 0.18532471702143027 1105 9 0.24674088203079236 1105 10-11 0.40707507880152605 1105 12-13 0.17788931989248624 1105 14-15 0.2718109036043188 1105 16-17 0.29793521068820183 1105 18-19 0.14529961660215918 1105 20-21 0.2941845630341291 1105 22-23 0.14737793893900175 1105 24-25 0.2025160417802212 1105 26-27 0.1222830756279194 1105 28-29 -0.006325499292792358 1105 30-31 0.10228115171973684 1105 32-33 0.12947315414500338 1105 34-35 -0.029769328546898066 1105 36-37 0.11600796983431394 1105 38-39 0.26344940521907745 1105 40-41 0.1963570190476247 1105 42-43 0.2248822581453851 1105 44-45 0.4338995966607584 1105 46-47 0.45264977743949686 1105 48-49 0.3242034339082309 1105 50-51 0.3363858695650066 1105 52-53 0.058810524201490466 1105 54-55 0.08162795738134321 1105 56-57 0.32416337271724416 1105 58-59 0.23083070705491338 1105 60-61 0.186017332675938 1105 62-63 0.23996268161779 1105 64-65 0.2792628154268044 1105 66-67 0.2768916359785081 1105 68-69 0.23300385367041088 1105 70-71 0.18062210184371708 1105 72-73 -0.1497695067525413 1105 74-75 0.038922289151123834 1105 76-77 0.18290876856963934 1105 78-79 0.2713536188631096 1105 80-81 0.21241923870005763 1105 82-83 0.309964054869063 1105 84-85 0.21033504534340963 1105 86-87 0.08845869954387808 1105 88-89 0.2177480397354934 1105 90-91 0.2145610202151289 1105 92-93 0.15438965894811219 1105 94-95 0.3476176539841376 1105 96-97 0.05632925817482359 1105 98-99 -0.00193496970257101 1105 100-101 -0.033526282606256785 1106 1 -0.016017780436254014 1106 2 0.08019819981682019 1106 3 0.069045744620297 1106 4 0.12587048755256092 1106 5 0.17883021837467084 1106 6 0.19955312404855619 1106 7 0.203190572673428 1106 8 0.21004853903778553 1106 9 0.1768960335956251 1106 10-11 0.08496034452676327 1106 12-13 0.1283637926436043 1106 14-15 0.22346387238954435 1106 16-17 0.2450812007273484 1106 18-19 0.18598847315357148 1106 20-21 0.18984462329114393 1106 22-23 0.20101590495732324 1106 24-25 0.22966654084766702 1106 26-27 0.032704054906879776 1106 28-29 0.15748339988943627 1106 30-31 -0.0034914292890562137 1106 32-33 0.16952357327968315 1106 34-35 0.18392991926520352 1106 36-37 0.22505984415539615 1106 38-39 0.17629193765333895 1106 40-41 0.06564028850306158 1106 42-43 0.06401549993178435 1106 44-45 0.10928645079369659 1106 46-47 0.08962175997726263 1106 48-49 0.015868904999202016 1106 50-51 -0.02057955438613135 1106 52-53 0.16633650235319664 1106 54-55 0.16883154000928613 1106 56-57 0.12472597647613526 1106 58-59 0.18902381643984256 1106 60-61 0.040557104355137596 1106 62-63 0.18887846526641283 1106 64-65 0.22009616583978442 1106 66-67 0.16121558916659495 1106 68-69 0.250300076334419 1106 70-71 0.3443305726682482 1106 72-73 0.13251923502360796 1106 74-75 0.15113663196024874 1106 76-77 0.39686421117175286 1106 78-79 0.4567238715736508 1106 80-81 0.5929269142959726 1106 82-83 0.6472485950506552 1106 84-85 0.6563681252147013 1106 86-87 0.4841197845800487 1106 88-89 0.504130774256204 1106 90-91 0.40704324029670147 1106 92-93 0.3963369443081177 1106 94-95 0.43067190579287384 1106 96-97 0.5364485424690564 1106 98-99 0.5911221514878129 1106 100-101 0.6760256839449497 1107 1 0.27723788643116265 1107 2 0.38565104193889965 1107 3 0.5354212160048064 1107 4 0.3843510914427952 1107 5 0.3212849363210637 1107 6 0.3265921925690307 1107 7 0.3913547472351766 1107 8 0.35966009877815 1107 9 0.4308683991849378 1107 10-11 0.4286495664158423 1107 12-13 0.47425532725785047 1107 14-15 0.5347864904272441 1107 16-17 0.6200906446069538 1107 18-19 0.517804849596331 1107 20-21 0.47143251181234547 1107 22-23 0.6398821645118389 1107 24-25 0.6291550603279674 1107 26-27 0.6638806219972864 1107 28-29 0.7130074758462897 1107 30-31 0.6814057025777274 1107 32-33 0.6836942495478056 1107 34-35 0.7227010924432875 1107 36-37 0.6972872829968253 1107 38-39 0.5629529703712493 1107 40-41 0.484013868953042 1107 42-43 0.5868157235628502 1107 44-45 0.5639056411887822 1107 46-47 0.5866256217145462 1107 48-49 0.5965385246321162 1107 50-51 0.6856515713459004 1107 52-53 0.6478928752990214 1107 54-55 0.7137876448568576 1107 56-57 0.7716711242647563 1107 58-59 0.7931973636473657 1107 60-61 0.8977683428956595 1107 62-63 0.8465993495157491 1107 64-65 0.7345517774470167 1107 66-67 0.521542228678058 1107 68-69 0.7030364269603169 1107 70-71 0.7040614382888535 1107 72-73 0.5867595909337453 1107 74-75 0.5840726747696294 1107 76-77 0.7350646502312088 1107 78-79 0.8268295135092316 1107 80-81 0.9039910398574804 1107 82-83 0.7783075183836203 1107 84-85 0.6783723647071689 1107 86-87 0.699819613834709 1107 88-89 0.6967578564967489 1107 90-91 0.8123346190599499 1107 92-93 0.915064967051137 1107 94-95 0.8095830210772803 1107 96-97 0.8652326240814467 1107 98-99 0.9686085900766948 1107 100-101 1.0041092995153704 1108 1 -0.11719851713877816 1108 2 -0.018749503118748123 1108 3 -0.0802118969706882 1108 4 0.08184822720409102 1108 5 0.07087660797238016 1108 6 0.1134711383225735 1108 7 0.11847053892728354 1108 8 0.14271041743439383 1108 9 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-0.5508004345875932 2316 74-75 -0.5067821782028261 2316 76-77 -0.5048919187155931 2316 78-79 -0.3947975838860316 2316 80-81 -0.3516701083789755 2316 82-83 -0.373197457158426 2316 84-85 -0.270936432319818 2316 86-87 -0.22236604272416471 2316 88-89 -0.4272691423950903 2316 90-91 -0.5122431034962105 2316 92-93 -0.4743044991274914 2316 94-95 -0.5833101810674073 2316 96-97 -0.5175539033372054 2316 98-99 -0.613103354582293 2316 100-101 -0.5550039458364964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13135.0 3 1761.0 4 1278.0 5 1268.0 6 1175.0 7 1323.0 8 1543.0 9 1740.0 10 1888.0 11 2194.0 12 2623.0 13 2971.0 14 3340.0 15 3601.0 16 3982.0 17 4924.0 18 4539.0 19 3128.0 20 3130.0 21 3343.0 22 3554.0 23 3867.0 24 4460.0 25 5267.0 26 6239.0 27 7333.0 28 8851.0 29 10628.0 30 13264.0 31 16896.0 32 21777.0 33 28518.0 34 41109.0 35 64643.0 36 113508.0 37 222176.0 38 323850.0 39 108547.0 40 114.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.94951348471032 20.22143669297189 14.062505863325416 23.76654395899237 2 15.881833090037114 21.833521590819537 39.27804817640415 23.006597142739203 3 18.287184702094606 25.83969995572133 30.916268283713706 24.95684705847036 4 13.321875430961292 18.575686418726917 38.227990367106315 29.874447783205483 5 13.963254937863962 37.02488068079486 35.15761016389506 13.854254217446119 6 30.013636295429308 37.18038174124426 18.94197728159419 13.864004681732242 7 25.72922528606265 32.51500656537235 23.91727630838492 17.83849184018008 8 24.040217235341725 34.36274100659377 22.376253037346586 19.220788720717916 9 25.13571985209373 16.563683064957036 22.15883888138146 36.141758201567775 10-11 23.56227208410653 26.469468529907907 29.950058933459108 20.01820045252645 12-13 24.422665716322168 24.81444836253142 29.098360655737704 21.66452526540871 14-15 22.177739527663196 26.700794732115007 26.808009699061753 24.31345604116004 16-17 21.748368848883405 28.83711003644585 27.664463654998055 21.750057459672693 18-19 21.54272259510978 28.210139727299588 29.187105126148566 21.060032551442067 20-21 22.275849519230317 27.71997640002889 28.851152280783378 21.153021799957415 22-23 22.2547113844682 27.57221543519194 28.37903049942903 21.794042680910835 24-25 22.075243264736557 27.66353317178204 28.656145201101353 21.605078362380056 26-27 22.027057633575435 27.6405482802586 28.67554895315909 21.656845133006872 28-29 21.995900511193405 27.652510409334567 28.689762673268117 21.661826406203915 30-31 22.401215377906567 27.515508728495263 28.43084021437916 21.652435679219007 32-33 22.093595735742046 27.63365192731856 28.363476600597178 21.90927573634222 34-35 22.427164205002427 27.65736635144546 28.292388967179633 21.62308047637248 36-37 22.36128221999097 27.424844055783034 28.307398516085435 21.906475208140566 38-39 22.194415840915614 27.539430667859577 28.417512111953275 21.848641379271537 40-41 22.44097534349867 27.418089499803287 28.307779311159788 21.833155845538258 42-43 22.471025525483988 27.45753763035993 28.071139561850895 22.000297282305183 44-45 22.546898646585795 27.62795858577853 27.846594610260887 21.978548157374792 46-47 22.496317441469476 27.46839283813297 27.837938348741226 22.197351371656328 48-49 22.407357725255057 27.448557084376134 27.946007451905942 22.198077738462864 50-51 22.440031871075032 27.638637534990885 27.742842256468357 22.178488337465723 52-53 22.404045661393894 27.766797723963794 27.708185337056392 22.12097127758592 54-55 22.457398051879547 27.66767081218572 27.637101027979323 22.23783010795541 56-57 22.298515623864255 27.821372146363903 27.674355459618244 22.205756770153595 58-59 22.40188125404432 27.70118445855333 27.732507432172632 22.164426855229717 60-61 22.426725035308152 27.70339464666528 27.793282729671787 22.076597588354783 62-63 22.339510670841786 27.653690225326987 27.75811508210035 22.248684021730874 64-65 22.36585020396716 27.672138620752403 27.68733415064023 22.27467702464021 66-67 22.262354909159804 27.660993326208246 27.633277698861967 22.44337406576998 68-69 22.331600118372 27.738489800263665 27.601358370293315 22.328551711071018 70-71 22.29945942295933 27.473064933018716 27.71559307791287 22.511882566109083 72-73 22.36768062904808 27.498532233649648 27.633960643228672 22.4998264940736 74-75 22.290455855683522 27.66425301518894 27.596550747631827 22.448740381495714 76-77 22.35265924094847 27.557274497858902 27.59258816340332 22.49747809778931 78-79 22.263960876342907 27.660909546239864 27.59928640970663 22.4758431677106 80-81 22.358813849212286 27.65087048828289 27.46839224521399 22.521923417290836 82-83 22.267012040722623 27.68174934491959 27.587057571566394 22.464181042791395 84-85 22.400098669139023 27.63312713171533 27.436117125520372 22.530657073625278 86-87 22.2966790373997 27.52193409289915 27.565926564464682 22.615460305236464 88-89 22.3233160699514 27.48258811921675 27.55030359417234 22.64379221665951 90-91 22.370940578991725 27.45133994249383 27.57318060885629 22.604538869658157 92-93 22.355363101397096 27.428782632596526 27.633332926828125 22.582521339178253 94-95 22.293250200586826 27.429773509221366 27.606983938517033 22.66999235167478 96-97 22.286191189536783 27.341505669763528 27.65123862706623 22.721064513633454 98-99 22.157662145846277 27.42407014500229 27.609748092229424 22.808519616922013 100-101 22.309258697223616 27.33688557234001 27.67673905772631 22.67711667271007 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2048.0 1 1177.5 2 294.0 3 274.5 4 214.0 5 182.0 6 239.0 7 400.0 8 590.5 9 599.0 10 459.0 11 355.0 12 371.5 13 414.0 14 430.0 15 475.0 16 534.0 17 569.0 18 618.0 19 703.5 20 806.0 21 930.0 22 1088.5 23 1332.5 24 1672.5 25 2154.5 26 3017.0 27 4359.0 28 6366.0 29 9451.0 30 13093.5 31 17419.5 32 21864.5 33 25226.5 34 28414.0 35 31178.5 36 33763.5 37 35654.0 38 36184.0 39 36522.0 40 36532.0 41 36588.5 42 37118.0 43 37916.0 44 39046.5 45 39986.0 46 40419.5 47 40316.5 48 40521.0 49 40289.0 50 38763.0 51 36768.5 52 34529.5 53 32616.5 54 30497.5 55 28078.0 56 25403.0 57 22356.0 58 19938.0 59 17784.5 60 14953.5 61 12292.5 62 10106.0 63 7908.0 64 5910.0 65 4395.5 66 3418.0 67 2668.0 68 2050.0 69 1623.5 70 1301.0 71 1076.0 72 863.0 73 670.0 74 545.5 75 421.5 76 315.5 77 258.0 78 208.5 79 153.0 80 107.0 81 83.5 82 64.0 83 47.0 84 31.0 85 25.0 86 23.5 87 16.5 88 11.5 89 10.0 90 10.0 91 8.5 92 5.0 93 4.5 94 4.5 95 4.0 96 3.0 97 1.5 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14417037397176732 2 0.14716806855727518 3 0.14154739120944798 4 0.14576289922031838 5 0.13480257839205537 6 0.11381871629350053 7 0.12056352911089314 8 0.1103526319290071 9 0.10641815778552807 10-11 0.09648829447103338 12-13 0.11306929264712356 14-15 0.13157068891705473 16-17 0.1425778487232163 18-19 0.13883073049133152 20-21 0.12965029082321378 22-23 0.12360806267429955 24-25 0.10857275076886182 26-27 0.11311613162502213 28-29 0.117706351459081 30-31 0.10791700507828197 32-33 0.10632447982973094 34-35 0.12220289333734276 36-37 0.12370174063009666 38-39 0.11100837761958693 40-41 0.11395923322719621 42-43 0.10871326770255749 44-45 0.12473219814386498 46-47 0.1229523169837197 48-49 0.1359267138616208 50-51 0.12332702880690818 52-53 0.10861958974676038 54-55 0.10065696350400519 56-57 0.12004830035400899 58-59 0.11025895397320996 60-61 0.10876010668045608 62-63 0.10960320828263014 64-65 0.12965029082321378 66-67 0.12192185946995138 68-69 0.12707414703879297 70-71 0.11419342811668902 72-73 0.11625434314422566 74-75 0.09864288745436714 76-77 0.1243574863206765 78-79 0.12449800325437217 80-81 0.12482587609966211 82-83 0.13082126527067778 84-85 0.12154714764676292 86-87 0.13072758731488063 88-89 0.11812790226016805 90-91 0.12557529974603907 92-93 0.13958015413770847 94-95 0.11620750416632708 96-97 0.1015937430619764 98-99 0.10534086129386118 100-101 0.14862007687213052 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1067487.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.69170032674784 #Duplication Level Percentage of deduplicated Percentage of total 1 67.58402407258643 41.69373359961715 2 19.52384798444899 24.08918758163214 3 7.130068344951298 13.195981189378998 4 2.775708409874943 6.849526856657551 5 1.251811372808755 3.8613186038466267 6 0.6182718185207597 2.2885343849193687 7 0.36077837778789285 1.5579922096802634 8 0.2023413918581159 0.9986227608166355 9 0.1288885008798511 0.7156215694659195 >10 0.41694345593771276 3.8780628684876355 >50 0.005336241549147504 0.23284953331729166 >100 0.0012184804598185813 0.11745324147385226 >500 4.5692900178435176E-4 0.21545583707541283 >1k 3.0461933452290125E-4 0.3056597636311897 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1851 0.17339789618046872 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1402 0.13133649402756192 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.2155080108703896E-4 0.0 24-25 0.0 0.0 0.0 7.96262624275518E-4 0.0 26-27 0.0 0.0 0.0 0.001639364226449596 0.0 28-29 0.0 0.0 0.0 0.0035129233423919918 0.0 30-31 0.0 0.0 0.0 0.008993083756523499 0.0 32-33 0.0 0.0 0.0 0.020562311297467793 0.0 34-35 0.0 0.0 0.0 0.03658124173877527 0.0 36-37 0.0 0.0 0.0 0.0587360782847941 0.0 38-39 0.0 0.0 0.0 0.08749521071450987 0.0 40-41 0.0 0.0 0.0 0.11812790226016805 0.0 42-43 0.0 0.0 0.0 0.1508683478112614 0.0 44-45 0.0 0.0 0.0 0.19569324966018323 0.0 46-47 0.0 0.0 0.0 0.24646670170222212 0.0 48-49 0.0 0.0 0.0 0.29180683230802806 0.0 50-51 0.0 0.0 0.0 0.33878632714028367 0.0 52-53 0.0 0.0 0.0 0.3853911101493508 0.0 54-55 0.0 0.0 0.0 0.4410358158928399 0.0 56-57 0.0 0.0 0.0 0.508296588155172 0.0 58-59 0.0 0.0 0.0 0.5734027674341702 0.0 60-61 0.0 0.0 0.0 0.6408040566301978 0.0 62-63 0.0 0.0 0.0 0.7163084890026764 0.0 64-65 0.0 0.0 0.0 0.8017896236675481 0.0 66-67 0.0 0.0 0.0 0.8983247571164801 0.0 68-69 0.0 0.0 0.0 1.0163589814208511 0.0 70-71 0.0 0.0 0.0 1.1331285533219608 0.0 72-73 0.0 0.0 0.0 1.254019955278144 0.0 74-75 0.0 0.0 0.0 1.3878389151343296 0.0 76-77 0.0 0.0 0.0 1.5327587127524738 0.0 78-79 0.0 0.0 0.0 1.6937911187677228 0.0 80-81 0.0 0.0 0.0 1.8657838456112348 0.0 82-83 0.0 0.0 0.0 2.0499547067083723 0.0 84-85 0.0 0.0 0.0 2.2676622759808787 0.0 86-87 0.0 0.0 0.0 2.4963301660816475 0.0 88-89 0.0 0.0 0.0 2.772820652616847 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1375 0.0 48.73027 1 GTATCAA 2445 0.0 38.871704 1 TCAACGC 2795 0.0 33.662426 4 ATCAACG 2835 0.0 33.18747 3 CAACGCA 2920 0.0 32.542282 5 TATCAAC 2925 0.0 32.005363 2 AACGCAG 3030 0.0 31.507334 6 ACGCAGA 3385 0.0 28.062704 7 CGCAGAG 3465 0.0 27.69154 8 GTATTCG 70 0.008373521 27.154661 1 GTGGTAT 805 0.0 22.43211 1 GCAGAGT 4020 0.0 22.332367 9 GAGTACG 130 2.932678E-4 21.932611 1 GAGTACT 2560 0.0 21.061314 12-13 GGACCGA 190 8.256138E-6 19.99837 6 CAGAGTA 3770 0.0 19.529877 10-11 AGAGTAC 3520 0.0 18.892723 10-11 GTACTTT 2745 0.0 18.690508 14-15 ACCGTGC 205 1.5890475E-5 18.536814 8 TGGTATC 845 0.0 17.995989 2 >>END_MODULE