FastQCFastQC Report
Fri 10 Feb 2017
SRR2031766_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031766_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067487
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT70190.6575255717399837No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT34000.31850504971020727RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT24040.22520180573627593RNA PCR Primer, Index 26 (100% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23530.22042422999062283No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG20670.19363233463264656RNA PCR Primer, Index 26 (100% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18110.16965077794858394No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT11410.10688654756451366RNA PCR Primer, Index 26 (100% over 27bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13650.050.4825441
GTATCAA23400.039.8057751
TCAACGC26650.034.051824
ATCAACG26950.033.8490563
CAACGCA26850.033.7870865
AACGCAG27600.032.868966
TATCAAC28650.031.8405632
ACGCAGA31400.028.891197
CGCAGAG32700.027.452118
TTGTACG1151.2794574E-424.7889164
GCAGAGT36950.023.9089349
CGGAGAT2704.0017767E-1122.8815521
TACGGCC1050.00219705822.6173867
CCTATAC2709.986252E-1021.1164863
CAGAGTA35650.020.18435110-11
GAGTACT25100.019.86905912-13
GTACATG19750.019.7311611
AGAGTAC32950.019.387810-11
TACATGG20450.016.9603062
TATACAC43150.016.8466973
Produced by FastQC (version 0.11.5)