##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031766_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1067487 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7483416659875 34.0 31.0 34.0 31.0 34.0 2 32.91414977418929 34.0 31.0 34.0 31.0 34.0 3 32.97701517676562 34.0 31.0 34.0 31.0 34.0 4 36.36576183129162 37.0 37.0 37.0 35.0 37.0 5 36.333753947354865 37.0 37.0 37.0 35.0 37.0 6 36.3568689829478 37.0 37.0 37.0 35.0 37.0 7 36.30285895753297 37.0 37.0 37.0 35.0 37.0 8 36.29258154900247 37.0 37.0 37.0 35.0 37.0 9 38.1503259524472 39.0 39.0 39.0 37.0 39.0 10-11 38.11026644820967 39.0 39.0 39.0 36.0 39.0 12-13 38.05761756349257 39.0 39.0 39.0 35.0 39.0 14-15 39.55664799665008 41.0 39.0 41.0 37.0 41.0 16-17 39.52700360753808 41.0 39.0 41.0 37.0 41.0 18-19 39.52911370349241 41.0 39.5 41.0 37.0 41.0 20-21 39.523410589543474 41.0 39.5 41.0 37.0 41.0 22-23 39.426946651340955 41.0 39.0 41.0 36.0 41.0 24-25 39.356965939632055 41.0 39.0 41.0 36.0 41.0 26-27 39.224057997896 41.0 39.0 41.0 36.0 41.0 28-29 39.12353077836077 40.5 39.0 41.0 36.0 41.0 30-31 38.96463329295813 40.0 38.5 41.0 35.0 41.0 32-33 38.75677923946615 40.0 38.0 41.0 35.0 41.0 34-35 38.599831660713434 40.0 38.0 41.0 35.0 41.0 36-37 38.50779353753254 40.0 38.0 41.0 34.5 41.0 38-39 38.559114068836436 40.0 38.0 41.0 35.0 41.0 40-41 38.493660344341436 40.0 38.0 41.0 35.0 41.0 42-43 38.39995896905536 40.0 38.0 41.0 34.5 41.0 44-45 38.22849692783144 40.0 38.0 41.0 34.0 41.0 46-47 38.043011296624684 40.0 38.0 41.0 33.5 41.0 48-49 37.839583994933896 40.0 38.0 41.0 33.0 41.0 50-51 37.67766633223636 40.0 38.0 41.0 33.0 41.0 52-53 37.769553633908416 40.0 38.0 41.0 33.0 41.0 54-55 37.68774561189036 40.0 37.0 41.0 33.0 41.0 56-57 37.474320998756895 40.0 37.0 41.0 33.0 41.0 58-59 37.254241972033384 40.0 36.5 41.0 33.0 41.0 60-61 36.94106953995693 39.5 36.0 41.0 32.0 41.0 62-63 36.62628303670209 39.0 35.5 41.0 31.5 41.0 64-65 36.251670512146745 39.0 35.0 41.0 31.0 41.0 66-67 35.831507549974845 38.5 35.0 40.5 30.5 41.0 68-69 35.393861470912526 37.5 35.0 40.0 30.0 41.0 70-71 35.04084499389688 37.0 35.0 39.5 30.0 41.0 72-73 34.658348532581655 36.5 35.0 39.0 29.0 41.0 74-75 34.27792282247934 36.0 35.0 39.0 29.0 40.5 76-77 33.34839393828683 35.0 33.5 37.0 27.5 39.0 78-79 33.538751759974595 35.0 34.0 37.0 29.0 39.0 80-81 33.308278227275835 35.0 34.0 37.0 29.0 39.0 82-83 33.026648099695834 35.0 34.0 36.0 29.0 38.5 84-85 32.72753579200496 35.0 34.0 36.0 28.0 37.0 86-87 32.46953967589301 35.0 34.0 36.0 27.0 37.0 88-89 32.2393860534133 35.0 34.0 35.0 27.0 36.5 90-91 32.03507677376867 35.0 34.0 35.0 26.0 36.0 92-93 31.868683178343154 35.0 34.0 35.0 26.0 36.0 94-95 31.689976552407664 35.0 33.5 35.0 25.5 36.0 96-97 31.525755348777082 35.0 33.0 35.0 25.0 35.5 98-99 31.321302741860087 35.0 33.0 35.0 24.5 35.0 100-101 30.631394574360158 34.5 32.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19902392613268205 1101 2 0.21697842182104665 1101 3 0.18826204757119314 1101 4 0.09275465100908065 1101 5 0.006453731512408467 1101 6 0.10803573031967773 1101 7 0.1383612421448035 1101 8 0.14635734168727765 1101 9 0.17845322622377324 1101 10-11 0.2075926347243353 1101 12-13 0.13768404648203614 1101 14-15 0.08226215514367397 1101 16-17 -0.02100295032053623 1101 18-19 0.09731374844911755 1101 20-21 0.12049234692799615 1101 22-23 -0.026951188191290498 1101 24-25 -0.08790236004728769 1101 26-27 0.053569500879959264 1101 28-29 0.061726492607014904 1101 30-31 0.06956409206423331 1101 32-33 0.04915649512552278 1101 34-35 -0.07529169391301593 1101 36-37 0.0863445475639466 1101 38-39 0.06385933141141464 1101 40-41 -0.3076201489720276 1101 42-43 -0.021539035727840883 1101 44-45 0.09729692338694917 1101 46-47 0.05108540632377867 1101 48-49 -0.11879121502597201 1101 50-51 -0.009888306274746128 1101 52-53 0.1314633785984114 1101 54-55 0.1891111638787848 1101 56-57 -0.038977754339661885 1101 58-59 0.06006263268860579 1101 60-61 0.0777387445384079 1101 62-63 0.32357861631307827 1101 64-65 0.3489620431911433 1101 66-67 -0.03808252591699812 1101 68-69 -0.025895674405127522 1101 70-71 0.021464724585406714 1101 72-73 0.08759782720559173 1101 74-75 0.062057548195518564 1101 76-77 0.13424219907764723 1101 78-79 0.009695686496066003 1101 80-81 0.14230480818927305 1101 82-83 0.2417940530048881 1101 84-85 0.25370275979305745 1101 86-87 0.27514425166554446 1101 88-89 0.3275916924994178 1101 90-91 0.1118532169804709 1101 92-93 -0.08857241195334353 1101 94-95 0.1824695175431259 1101 96-97 0.09764149329943095 1101 98-99 -0.08012694160616718 1101 100-101 -0.4778646586320221 1102 1 0.15675078707182877 1102 2 0.10183171086428189 1102 3 0.1265616600328201 1102 4 0.06672666771852676 1102 5 0.13771348288388907 1102 6 0.07403678724252671 1102 7 0.14698774574337392 1102 8 0.06789466112962828 1102 9 0.16221813638533433 1102 10-11 0.23784516378971432 1102 12-13 0.13871140065185017 1102 14-15 0.22508829133573727 1102 16-17 0.14293899441749147 1102 18-19 0.09444873259527498 1102 20-21 0.21466228502251994 1102 22-23 0.10155867642192362 1102 24-25 0.24496794042078562 1102 26-27 0.2433701954292573 1102 28-29 0.22319473459202044 1102 30-31 0.12496320626222968 1102 32-33 0.2671537521590963 1102 34-35 0.23822119047976287 1102 36-37 0.20652988653419868 1102 38-39 0.05656027257604279 1102 40-41 0.18907004681035033 1102 42-43 0.359696179934744 1102 44-45 0.21078527207083653 1102 46-47 0.41116454971298566 1102 48-49 0.2425889500553069 1102 50-51 0.1724044613532172 1102 52-53 0.3001967068987881 1102 54-55 0.39713559892836514 1102 56-57 0.4693014746099564 1102 58-59 0.5160971588348673 1102 60-61 0.5985934679769258 1102 62-63 0.7260510608246236 1102 64-65 0.638531221307808 1102 66-67 0.6628385068476135 1102 68-69 0.7772241353487672 1102 70-71 0.6661039867526029 1102 72-73 0.7207059353137097 1102 74-75 0.6575719173132413 1102 76-77 0.6111830365631761 1102 78-79 0.746434450399633 1102 80-81 0.6049803328759751 1102 82-83 0.628730989941829 1102 84-85 0.7374179694160858 1102 86-87 0.828187937809794 1102 88-89 0.7836677406145682 1102 90-91 0.6919421746532279 1102 92-93 0.7781999469977876 1102 94-95 0.6860026596237532 1102 96-97 0.7204087437901414 1102 98-99 0.5791324574577033 1102 100-101 0.6240560469898 1103 1 0.16138225220068136 1103 2 0.15424956393089673 1103 3 0.07500282060229324 1103 4 0.05644253243890063 1103 5 -0.04422920949311049 1103 6 0.09774075792793013 1103 7 0.06559169840752332 1103 8 0.08254594965008266 1103 9 0.07610652468352441 1103 10-11 0.10001852835990377 1103 12-13 -0.05100746761270614 1103 14-15 -0.03550520899465681 1103 16-17 0.017565053166819666 1103 18-19 0.03184586120703159 1103 20-21 0.009409086247963216 1103 22-23 0.11992835263986024 1103 24-25 -0.03788782938759283 1103 26-27 0.16456921026676952 1103 28-29 0.10213988987525369 1103 30-31 0.11686865469138041 1103 32-33 -0.0012249346913861814 1103 34-35 0.034992380483970464 1103 36-37 -0.011079893005657482 1103 38-39 -0.05355566422938551 1103 40-41 -0.06167783569100749 1103 42-43 0.05666860739915336 1103 44-45 0.012278033529355525 1103 46-47 -0.02673717432137579 1103 48-49 0.20713511452938604 1103 50-51 0.2550710078781151 1103 52-53 0.18210636028978655 1103 54-55 0.40856408454138915 1103 56-57 0.19968833110061723 1103 58-59 0.2142619933395764 1103 60-61 0.13471709385547115 1103 62-63 0.07817757010557358 1103 64-65 0.3548614910260781 1103 66-67 0.3672356955302618 1103 68-69 0.3867423668619452 1103 70-71 0.38459680828200504 1103 72-73 0.46210378477606895 1103 74-75 0.41948998062795084 1103 76-77 0.3812016585371083 1103 78-79 0.42398295763817373 1103 80-81 0.3047430528855841 1103 82-83 0.41421486090956705 1103 84-85 0.3096712282615286 1103 86-87 0.27390188026188866 1103 88-89 0.31213833591701956 1103 90-91 0.525377628488755 1103 92-93 0.4996722843558672 1103 94-95 0.4577092734280441 1103 96-97 0.3629749719393587 1103 98-99 0.605059050837891 1103 100-101 0.642365652324095 1104 1 0.06931123024351393 1104 2 0.07448088226468741 1104 3 0.1639225820552639 1104 4 0.08631964457407548 1104 5 0.07886648039574595 1104 6 0.086658260412797 1104 7 0.11001882409650676 1104 8 0.10804066366471687 1104 9 0.04910959688014316 1104 10-11 0.09975422909181475 1104 12-13 0.2464545817231567 1104 14-15 0.12826771953159266 1104 16-17 0.13499863950458035 1104 18-19 0.11492957856494712 1104 20-21 0.31152686884487224 1104 22-23 0.2684487285321566 1104 24-25 0.18832975442013122 1104 26-27 0.1707339342208627 1104 28-29 0.2496618018658623 1104 30-31 0.2267911720854343 1104 32-33 0.17744024105632406 1104 34-35 -0.026669542349679887 1104 36-37 -0.16684929754166689 1104 38-39 0.024530086199817447 1104 40-41 -0.10002494872976797 1104 42-43 -0.06991041130510212 1104 44-45 -0.1371549654766966 1104 46-47 -0.14971991580507193 1104 48-49 -0.14012136870906744 1104 50-51 -0.07430461333810712 1104 52-53 -0.12828376350166337 1104 54-55 -0.10318480856424372 1104 56-57 -0.08230995208362657 1104 58-59 0.06166192105260393 1104 60-61 0.03355485476627962 1104 62-63 0.11921511415545893 1104 64-65 0.16656746373774212 1104 66-67 0.1453293523478223 1104 68-69 0.23223798446382915 1104 70-71 0.2588502193238469 1104 72-73 0.1831008932969027 1104 74-75 0.20177289379321195 1104 76-77 0.347728269181367 1104 78-79 0.5336897435783641 1104 80-81 0.3785073973330455 1104 82-83 0.46286964540401243 1104 84-85 0.46831968764527687 1104 86-87 0.3649315179234023 1104 88-89 0.4195838947857382 1104 90-91 0.34864725892157367 1104 92-93 0.5144018858738946 1104 94-95 0.5974422633982215 1104 96-97 0.5989332262088034 1104 98-99 0.47192563618170524 1104 100-101 0.291870938309458 1105 1 0.14947669849266987 1105 2 0.17741772710571269 1105 3 0.09412486915567086 1105 4 0.057085476680114766 1105 5 0.0337222371314283 1105 6 0.02126364895996602 1105 7 0.11009895924334501 1105 8 0.059998560225402287 1105 9 0.061296636644506464 1105 10-11 0.12264905712611807 1105 12-13 0.1680813252040565 1105 14-15 0.2114241779912689 1105 16-17 0.18035188389512058 1105 18-19 0.15198775234991757 1105 20-21 0.2279491594105849 1105 22-23 0.2952636813173939 1105 24-25 0.19315085811934551 1105 26-27 0.26497936435168157 1105 28-29 0.22574037762606025 1105 30-31 0.2609103352146036 1105 32-33 0.16768649419675796 1105 34-35 0.27753755285953474 1105 36-37 0.4245480861540827 1105 38-39 0.26685227280641755 1105 40-41 0.18193706287899403 1105 42-43 0.009146159776939555 1105 44-45 0.1545737878803095 1105 46-47 0.1644445128529881 1105 48-49 0.13035316130912378 1105 50-51 0.12537917770407603 1105 52-53 0.22945144398339323 1105 54-55 0.2179643727577698 1105 56-57 0.10927152586838673 1105 58-59 0.22705436669065193 1105 60-61 0.3345175594357812 1105 62-63 0.21480876398651816 1105 64-65 0.10021123052280956 1105 66-67 0.07739532306600694 1105 68-69 0.21872330023962405 1105 70-71 0.24319895881964015 1105 72-73 0.1547265538704039 1105 74-75 0.24560681911592042 1105 76-77 0.17587519805600493 1105 78-79 0.33005767219310655 1105 80-81 0.21200388871309883 1105 82-83 0.2642980002511024 1105 84-85 0.43063342757423584 1105 86-87 0.21665767230268074 1105 88-89 0.22087905202388214 1105 90-91 0.22941265786464982 1105 92-93 0.2024325104948801 1105 94-95 0.21108473069442368 1105 96-97 0.22479738630583768 1105 98-99 0.2425604364935836 1105 100-101 0.11955221700650043 1106 1 0.1103465310437528 1106 2 0.014387660085155574 1106 3 -0.015114539751714062 1106 4 0.02362438754482099 1106 5 0.07949297353773233 1106 6 0.07045714776097611 1106 7 0.058560765724053 1106 8 0.13010575212555153 1106 9 0.0416188272124387 1106 10-11 0.0402352922924436 1106 12-13 0.05503691127526622 1106 14-15 0.02089723654714959 1106 16-17 0.10316756711539199 1106 18-19 0.2862409957759624 1106 20-21 0.21141639722083738 1106 22-23 0.15764506312961402 1106 24-25 0.255953424166357 1106 26-27 0.19484347863505036 1106 28-29 0.14567438184815273 1106 30-31 0.1689251330256738 1106 32-33 0.1686098202199986 1106 34-35 0.12715018633701902 1106 36-37 0.1576699733873781 1106 38-39 0.07438849874972675 1106 40-41 -0.006576187515541676 1106 42-43 -0.03307679117713036 1106 44-45 0.016851666997361292 1106 46-47 -0.061530564517838116 1106 48-49 -0.09282426895465079 1106 50-51 -0.06527893547252717 1106 52-53 0.08097016669864843 1106 54-55 -0.09121252462084328 1106 56-57 0.05884521773778317 1106 58-59 0.08779712662588679 1106 60-61 0.06019647562933983 1106 62-63 0.13877527420357438 1106 64-65 0.21070599390129985 1106 66-67 0.021882011184722217 1106 68-69 0.05894998138983709 1106 70-71 0.011787428004531364 1106 72-73 -0.020248339040172425 1106 74-75 0.042286534637227646 1106 76-77 -0.0256240538552035 1106 78-79 -0.06627976708873007 1106 80-81 -0.05640831845625627 1106 82-83 -0.08667656473014063 1106 84-85 0.05910794131652608 1106 86-87 0.0279763581258905 1106 88-89 -0.06921440783550992 1106 90-91 0.06587122604320683 1106 92-93 0.15771114794850405 1106 94-95 0.14883349583553596 1106 96-97 0.15585639308987354 1106 98-99 0.14046168877371912 1106 100-101 0.17164481605140836 1107 1 0.25307431202345754 1107 2 0.17465515392774478 1107 3 0.11143236772849008 1107 4 0.09543241020149651 1107 5 0.04348750795986689 1107 6 0.09482667435818115 1107 7 0.13134771395863964 1107 8 0.13745470941331206 1107 9 0.13184187545932957 1107 10-11 0.11809495300352779 1107 12-13 0.1779109590623733 1107 14-15 0.1897338369231747 1107 16-17 0.22516198497305595 1107 18-19 0.14724111881622548 1107 20-21 0.08277078986605346 1107 22-23 0.1239001925053671 1107 24-25 0.10394257935465845 1107 26-27 0.22779992022437767 1107 28-29 0.19635725529153092 1107 30-31 0.18523228468682618 1107 32-33 0.23995050574852428 1107 34-35 0.21904722970116097 1107 36-37 0.18308318359411402 1107 38-39 0.23664082626131488 1107 40-41 0.36784827547847954 1107 42-43 0.3572079930599017 1107 44-45 0.3937725521101356 1107 46-47 0.5185799774421298 1107 48-49 0.6136988075699108 1107 50-51 0.6325061239861327 1107 52-53 0.539100861358051 1107 54-55 0.5552507154825079 1107 56-57 0.630329989886647 1107 58-59 0.5149222153983573 1107 60-61 0.7372616632225686 1107 62-63 0.9381316872993679 1107 64-65 0.7681377335394757 1107 66-67 0.8530610606663274 1107 68-69 0.6958576342781484 1107 70-71 0.8297353323266989 1107 72-73 0.8310208104494237 1107 74-75 0.8656897634726803 1107 76-77 0.8669779886131153 1107 78-79 0.8067453827721494 1107 80-81 0.9104328711056127 1107 82-83 0.7908373960221695 1107 84-85 0.9047852347761847 1107 86-87 0.8272064930600393 1107 88-89 0.9037831119318795 1107 90-91 0.7288731277707399 1107 92-93 0.7463308952626662 1107 94-95 0.8264669351561835 1107 96-97 0.8888514736380735 1107 98-99 1.0455480004368525 1107 100-101 1.0112353941895265 1108 1 -0.038982991346166784 1108 2 -0.09472171790002193 1108 3 -0.06335695963772281 1108 4 0.016895966444401722 1108 5 9.934662995263466E-4 1108 6 -0.060230656904842306 1108 7 -0.08652968112861004 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12 1505.0 13 2131.0 14 2492.0 15 3079.0 16 3592.0 17 4942.0 18 5145.0 19 3488.0 20 3229.0 21 3681.0 22 4663.0 23 4870.0 24 5701.0 25 6373.0 26 6704.0 27 5790.0 28 7401.0 29 9083.0 30 11654.0 31 15074.0 32 19667.0 33 26413.0 34 37151.0 35 58986.0 36 105308.0 37 223525.0 38 361811.0 39 121606.0 40 123.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.66938259991267 20.77042431147532 14.459065176776148 24.10112791183586 2 15.512507412268253 21.79764250056441 39.73294288361357 22.95690720355377 3 17.850729154663977 26.144646823467472 31.67736018563537 24.327263836233186 4 12.854171351472903 19.194588111835696 37.66078255003373 30.29045798665767 5 13.286438148661295 37.23267824338844 35.761184913727284 13.719698694222974 6 29.452442980570254 37.59146481409141 18.734279668042795 14.221812537295536 7 24.901286854078787 32.796746002527435 23.74492616771914 18.557040975674646 8 23.560567950710407 34.72201535006983 22.642430305942835 19.074986393276923 9 24.39364601161419 16.30652176560464 21.986965649230388 37.31286657355078 10-11 23.05176693101037 26.45274670920897 30.238415193072697 20.257071166707963 12-13 24.00564316270042 24.644433442483816 29.310909318291145 22.039014076524623 14-15 21.696855182711914 26.615043843645797 26.77059616210603 24.917504811536258 16-17 21.47952289169042 29.13569202016914 27.607924325919402 21.77686076222104 18-19 21.2375150587455 27.967954614861462 29.432376630700542 21.362153695692495 20-21 21.748929963549905 27.334009688174188 29.084710165088662 21.832350183187245 22-23 21.57066081366799 27.709377257053248 28.83042135407738 21.889540575201387 24-25 21.776237087664768 27.70741001998151 29.03693440763213 21.479418484721595 26-27 21.58153486428811 27.65550145107833 29.272484219874435 21.490479464759122 28-29 21.25031969279513 27.293942184553384 29.635498687971403 21.820239434680087 30-31 21.760706931987865 27.046771573585037 28.947452238249493 22.2450692561776 32-33 21.951590798167278 27.269333563157446 29.174609806432517 21.604465832242763 34-35 21.99301913016919 27.75001159418389 28.977870801909972 21.279098473736948 36-37 21.799178780574405 27.405926532432357 28.68515893678034 22.109735750212895 38-39 21.726587771092294 27.14688797146944 29.105787705143015 22.02073655229525 40-41 21.88649604163798 27.090962231858562 29.236327936546303 21.786213789957163 42-43 22.0679034030391 27.059392760754932 29.078808453873446 21.793895382332526 44-45 21.76059286904665 27.48942141684161 28.85281038551289 21.89717532859885 46-47 22.222097318281158 26.99690956423825 28.438051236221146 22.342941881259442 48-49 21.996801834589085 27.01676929086724 28.552244664337834 22.43418421020584 50-51 21.894927057659718 27.324969765439768 28.594633939336028 22.185469237564487 52-53 21.837127758932894 27.595933252582938 28.79594786634404 21.770991122140128 54-55 21.7783448416702 27.572841636478945 28.36039221086533 22.28842131098552 56-57 22.198771507287677 27.97354909240112 27.71588787498115 22.11179152533005 58-59 21.62228496592917 28.190686119297563 28.48600725820201 21.70102165657126 60-61 22.07699683219832 28.55035717982743 27.814169998955464 21.55847598901879 62-63 21.56529511128683 28.878924691088525 27.748771052777045 21.8070091448476 64-65 21.753048137860613 28.67137256371463 27.70613281121921 21.86944648720555 66-67 21.646020716835874 28.6591483744769 27.694551736348892 22.00027917233833 68-69 21.661813220141934 28.439900717079748 27.72951915046496 22.16876691231336 70-71 21.841846661726695 28.059931633571683 27.822082412553645 22.276139292147977 72-73 21.828703497683406 28.14747708581501 27.767256429485858 22.256562987015723 74-75 21.84422178815175 27.999653368183886 27.741365823039775 22.41475902062459 76-77 21.889138337491936 28.00591681885738 27.623468861522927 22.481475982127762 78-79 21.887368487120614 27.900356071911347 27.642318692323332 22.569956748644707 80-81 21.817188752311104 27.908008373606368 27.696560291511673 22.57824258257085 82-83 21.91214138624325 27.94386035260368 27.569249391497262 22.57474886965581 84-85 22.048716419812013 27.83673633805746 27.53190155916338 22.582645682967144 86-87 21.906088592256314 27.69162599002214 27.627220977579093 22.77506444014245 88-89 21.96141104084708 27.631439046016254 27.63036174397402 22.77678816916265 90-91 21.987668233898866 27.54436353791662 27.610687530620982 22.85728069756353 92-93 21.91623879260356 27.50665816070828 27.764881445863043 22.812221600825115 94-95 21.959564847159733 27.421879610711887 27.640758154431854 22.977797387696526 96-97 21.945497151951948 27.33245556965411 27.697097524978094 23.024949753415854 98-99 21.907162291982146 27.333647779152244 27.66873398398949 23.09045594487612 100-101 21.8922325545013 27.479391779382084 27.49447410687562 23.133901559240993 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1155.0 1 684.5 2 235.5 3 226.5 4 169.5 5 168.5 6 240.0 7 398.5 8 603.0 9 627.0 10 467.0 11 342.0 12 336.5 13 387.0 14 446.0 15 497.0 16 534.5 17 592.0 18 664.0 19 737.5 20 840.0 21 976.5 22 1125.0 23 1370.0 24 1740.5 25 2248.0 26 3126.5 27 4581.0 28 6599.0 29 9434.0 30 13277.5 31 17528.0 32 21743.5 33 25655.5 34 28747.0 35 31576.5 36 34435.5 37 36150.5 38 36955.0 39 37484.5 40 38268.0 41 38967.0 42 39761.5 43 40948.5 44 41742.0 45 42511.0 46 42591.5 47 42247.5 48 41849.0 49 40812.5 50 38994.5 51 36598.5 52 34067.5 53 31654.0 54 29073.0 55 25923.5 56 22932.5 57 20101.0 58 17703.0 59 15537.0 60 13003.0 61 10753.5 62 8777.5 63 6851.5 64 5064.5 65 3701.0 66 2817.5 67 2234.5 68 1716.5 69 1309.0 70 1075.5 71 875.0 72 665.5 73 506.5 74 394.5 75 268.0 76 186.5 77 136.5 78 103.0 79 77.5 80 48.5 81 35.5 82 28.5 83 18.5 84 12.5 85 9.0 86 3.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019203980938409554 2 0.0 3 0.0016862032043481559 4 0.019391336850003795 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.6838977898559886E-5 12-13 4.6838977898559886E-5 14-15 4.6838977898559886E-5 16-17 0.0013583303590582367 18-19 9.367795579711977E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.683897789855989E-4 28-29 0.0052459655246387075 30-31 9.367795579711977E-5 32-33 3.747118231884791E-4 34-35 0.013255430745292448 36-37 0.004683897789855989 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.683897789855989E-4 60-61 0.002576143784420794 62-63 0.010304575137683175 64-65 0.0029976945855078327 66-67 0.00430918596666751 68-69 0.003044533563406393 70-71 3.747118231884791E-4 72-73 0.0075410754416681425 74-75 0.006744812817392623 76-77 0.002014076049638075 78-79 5.620677347827187E-4 80-81 0.008009465220653742 82-83 0.010585609005074536 84-85 0.002201431961232315 86-87 0.002201431961232315 88-89 5.152287568841588E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 1.4051693369567967E-4 98-99 4.6838977898559886E-5 100-101 0.0011709744474639973 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1067487.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.79636940287032 #Duplication Level Percentage of deduplicated Percentage of total 1 65.67580624855628 38.61498965021454 2 20.511143629305096 24.11961555407905 3 7.464291554044765 13.166197306270227 4 3.05710531484662 7.189867735808002 5 1.3575667564254121 3.9909998249922496 6 0.6854283120022364 2.4180417739001605 7 0.3801847344216214 1.5647437460470057 8 0.22230323071941266 1.0456498298264076 9 0.1596890646599217 0.8450223511809241 >10 0.47545479294423654 4.231571202213077 >50 0.006076626619515015 0.23405682124165678 >100 0.0031933787990475375 0.3761751566768542 >500 4.7900636067221494E-4 0.20916341406831068 >1k 0.0011176815082351683 1.3349657756065432 >5k 1.5966878689073832E-4 0.6589398578749878 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 7019 0.6575255717399837 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 3400 0.31850504971020727 RNA PCR Primer, Index 26 (100% over 22bp) ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 2404 0.22520180573627593 RNA PCR Primer, Index 26 (100% over 24bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2353 0.22042422999062283 No Hit TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 2067 0.19363233463264656 RNA PCR Primer, Index 26 (100% over 29bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1811 0.16965077794858394 No Hit CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT 1141 0.10688654756451366 RNA PCR Primer, Index 26 (100% over 27bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.6838977898559886E-5 0.0 14-15 0.0 0.0 0.0 9.367795579711977E-5 0.0 16-17 0.0 0.0 0.0 9.367795579711977E-5 0.0 18-19 0.0 0.0 0.0 9.367795579711977E-5 0.0 20-21 0.0 0.0 0.0 9.367795579711977E-5 0.0 22-23 0.0 0.0 0.0 5.152287568841588E-4 0.0 24-25 0.0 0.0 0.0 8.899405800726379E-4 0.0 26-27 0.0 0.0 0.0 0.001639364226449596 0.0 28-29 0.0 0.0 0.0 0.0035129233423919918 0.0 30-31 0.0 0.0 0.0 0.008993083756523499 0.0 32-33 0.0 0.0 0.0 0.020281277430076433 0.0 34-35 0.0 0.0 0.0 0.03630020787138391 0.0 36-37 0.0 0.0 0.0 0.058970273174286894 0.0 38-39 0.0 0.0 0.0 0.08768256662610412 0.0 40-41 0.0 0.0 0.0 0.11822158021596516 0.0 42-43 0.0 0.0 0.0 0.15072783087756572 0.0 44-45 0.0 0.0 0.0 0.19480330908011056 0.0 46-47 0.0 0.0 0.0 0.2451083713431639 0.0 48-49 0.0 0.0 0.0 0.2904016629710713 0.0 50-51 0.0 0.0 0.0 0.3373343188254283 0.0 52-53 0.0 0.0 0.0 0.384407491613481 0.0 54-55 0.0 0.0 0.0 0.4404737481580572 0.0 56-57 0.0 0.0 0.0 0.5069382577961137 0.0 58-59 0.0 0.0 0.0 0.5721381150309091 0.0 60-61 0.0 0.0 0.0 0.6395862432048353 0.0 62-63 0.0 0.0 0.0 0.7155122263784008 0.0 64-65 0.0 0.0 0.0 0.8011807169548669 0.0 66-67 0.0 0.0 0.0 0.8982310791606829 0.0 68-69 0.0 0.0 0.0 1.0164994983545468 0.0 70-71 0.0 0.0 0.0 1.1334564261672506 0.0 72-73 0.0 0.0 0.0 1.2551909297256079 0.0 74-75 0.0 0.0 0.0 1.390227703007156 0.0 76-77 0.0 0.0 0.0 1.5365058309843587 0.0 78-79 0.0 0.0 0.0 1.69875605042497 0.0 80-81 0.0 0.0 0.0 1.869999353622105 0.0 82-83 0.0 0.0 0.0 2.054779121431924 9.367795579711977E-5 84-85 0.0 0.0 0.0 2.274547605731967 9.367795579711977E-5 86-87 0.0 0.0 0.0 2.5037307245896203 9.367795579711977E-5 88-89 0.0 0.0 0.0 2.781954253307066 9.367795579711977E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1365 0.0 50.482544 1 GTATCAA 2340 0.0 39.805775 1 TCAACGC 2665 0.0 34.05182 4 ATCAACG 2695 0.0 33.849056 3 CAACGCA 2685 0.0 33.787086 5 AACGCAG 2760 0.0 32.86896 6 TATCAAC 2865 0.0 31.840563 2 ACGCAGA 3140 0.0 28.89119 7 CGCAGAG 3270 0.0 27.45211 8 TTGTACG 115 1.2794574E-4 24.788916 4 GCAGAGT 3695 0.0 23.908934 9 CGGAGAT 270 4.0017767E-11 22.881552 1 TACGGCC 105 0.002197058 22.617386 7 CCTATAC 270 9.986252E-10 21.116486 3 CAGAGTA 3565 0.0 20.184351 10-11 GAGTACT 2510 0.0 19.869059 12-13 GTACATG 1975 0.0 19.731161 1 AGAGTAC 3295 0.0 19.3878 10-11 TACATGG 2045 0.0 16.960306 2 TATACAC 4315 0.0 16.846697 3 >>END_MODULE