FastQCFastQC Report
Fri 10 Feb 2017
SRR2031764_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031764_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455073
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44330.3046582542594083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40490.27826782573795267No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22830.15689934456896665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.13098999156743338No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18950.13023401575041252No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14630.10054478366377494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62400.027.563091
GTATCAA84700.024.1206471
GAGTACT41300.022.08983412-13
TCAACGC91250.022.0232544
ATCAACG93450.021.5047843
CAACGCA93550.021.4817965
TATCAAC94500.021.3175832
GTACATG50000.021.2854061
AACGCAG94500.021.2075376
TACATGG51100.019.9903982
GTACTTT45450.019.65498514-15
ACATGGG51150.019.5050753
ACGCAGA104150.019.060827
CGCAGAG104150.018.8367088
AGAGTAC70000.018.39332410-11
CATGGGG28000.017.476454
GCAGAGT111000.016.861169
GTGTAGC8700.016.383471
AGTACTT45600.016.3597312-13
ACCGTAT1750.0021694216.2864888
Produced by FastQC (version 0.11.5)