##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031764_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1455073 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01856058080935 33.0 31.0 34.0 30.0 34.0 2 32.313431009990566 34.0 31.0 34.0 31.0 34.0 3 32.412314708609124 34.0 31.0 34.0 31.0 34.0 4 35.83202354795945 37.0 35.0 37.0 35.0 37.0 5 35.835696215928685 37.0 37.0 37.0 35.0 37.0 6 35.872653811870606 37.0 37.0 37.0 35.0 37.0 7 35.85731918604771 37.0 37.0 37.0 35.0 37.0 8 35.8481773766677 37.0 37.0 37.0 35.0 37.0 9 37.62734790625625 39.0 38.0 39.0 35.0 39.0 10-11 37.56017808041246 39.0 38.0 39.0 35.0 39.0 12-13 37.51104411943594 39.0 38.0 39.0 35.0 39.0 14-15 38.89595504830342 41.0 39.0 41.0 36.0 41.0 16-17 38.8232171169419 41.0 39.0 41.0 36.0 41.0 18-19 38.84654378165219 41.0 39.0 41.0 36.0 41.0 20-21 38.85235929743731 41.0 39.0 41.0 35.5 41.0 22-23 38.74862841933017 41.0 39.0 41.0 35.0 41.0 24-25 38.678570422239986 41.0 39.0 41.0 35.0 41.0 26-27 38.557980939787896 40.5 38.5 41.0 35.0 41.0 28-29 38.44525910383878 40.0 38.0 41.0 34.0 41.0 30-31 38.277244165756635 40.0 38.0 41.0 34.0 41.0 32-33 38.06835876962874 40.0 38.0 41.0 34.0 41.0 34-35 37.89525886330102 40.0 38.0 41.0 33.5 41.0 36-37 37.7202466817816 40.0 38.0 41.0 33.0 41.0 38-39 37.52240746684187 40.0 38.0 41.0 33.0 41.0 40-41 37.41871713652854 40.0 37.5 41.0 33.0 41.0 42-43 37.37138136712041 40.0 38.0 41.0 33.0 41.0 44-45 37.2078854462972 40.0 37.0 41.0 32.0 41.0 46-47 37.090081391105464 40.0 37.0 41.0 31.5 41.0 48-49 37.09141671929862 40.0 37.0 41.0 32.0 41.0 50-51 36.52756803266915 39.5 36.5 40.5 30.5 41.0 52-53 36.53639920471344 39.5 37.0 40.5 31.0 41.0 54-55 36.745798320771534 40.0 37.0 41.0 31.0 41.0 56-57 36.595414113243805 40.0 36.0 41.0 31.0 41.0 58-59 36.3999692798918 40.0 36.0 41.0 30.5 41.0 60-61 36.14744174347266 39.0 35.0 41.0 30.0 41.0 62-63 35.85832119763064 39.0 35.0 41.0 29.5 41.0 64-65 35.58005474639417 39.0 35.0 41.0 29.0 41.0 66-67 35.27560507273518 38.0 35.0 40.5 29.0 41.0 68-69 34.93017979166681 37.0 35.0 40.0 28.5 41.0 70-71 34.56104573447517 37.0 35.0 39.5 28.0 41.0 72-73 34.191206901646865 36.0 34.5 39.0 28.0 41.0 74-75 33.795596165965556 36.0 34.0 39.0 27.0 41.0 76-77 33.418201698471485 35.0 34.0 37.5 26.0 39.5 78-79 33.03743935871259 35.0 34.0 37.0 26.0 39.0 80-81 32.6884286905193 35.0 34.0 37.0 26.0 39.0 82-83 32.36476657872148 35.0 34.0 36.0 25.0 38.0 84-85 32.04647876773193 35.0 34.0 36.0 25.0 37.0 86-87 31.787737797347624 35.0 33.0 35.5 24.0 37.0 88-89 31.513646737998712 35.0 33.0 35.0 23.5 36.5 90-91 31.275345979205166 35.0 33.0 35.0 21.0 36.0 92-93 31.0686965533688 35.0 33.0 35.0 20.0 36.0 94-95 30.816568653256574 35.0 33.0 35.0 18.5 36.0 96-97 30.559022811913906 35.0 32.0 35.0 10.0 35.0 98-99 30.324617390330246 35.0 32.0 35.0 2.0 35.0 100-101 29.736436247528474 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.033465253326319555 1101 2 0.16063546576856425 1101 3 0.1969149348997803 1101 4 0.3285742176665565 1101 5 0.20917887687612335 1101 6 0.21697781363272384 1101 7 0.26874641633817475 1101 8 0.22447075402186556 1101 9 0.2869199117847856 1101 10-11 0.1495735093291728 1101 12-13 0.05667065975835328 1101 14-15 0.13310357861460176 1101 16-17 0.26908761585998775 1101 18-19 0.3637135183634541 1101 20-21 0.26398426971690725 1101 22-23 0.16475565936012515 1101 24-25 0.015456359973882172 1101 26-27 -0.08504262012780117 1101 28-29 -0.05069698842210357 1101 30-31 0.03706668865832086 1101 32-33 -0.07900841860399765 1101 34-35 0.05735739653351857 1101 36-37 -0.20977077572253933 1101 38-39 -0.15505113554953454 1101 40-41 -0.29615033595082707 1101 42-43 -0.3358952123515522 1101 44-45 -0.35201765777700444 1101 46-47 -0.40973329046395435 1101 48-49 -0.14528449441025515 1101 50-51 -0.11671735585040466 1101 52-53 -0.19672884371102128 1101 54-55 -0.02602866638486745 1101 56-57 -0.08872010815125009 1101 58-59 -0.5173960301423719 1101 60-61 -0.48387739142928865 1101 62-63 -0.30728023483179356 1101 64-65 -0.22349204372820708 1101 66-67 -0.39730016198222273 1101 68-69 -0.5761183164697883 1101 70-71 -0.4736008802333913 1101 72-73 -0.2798841457087491 1101 74-75 -0.29082683920023555 1101 76-77 -0.36817756491247167 1101 78-79 -0.5503889849498051 1101 80-81 -0.5654838242784024 1101 82-83 -0.7308863530178229 1101 84-85 -0.7259903610845093 1101 86-87 -0.8830946718857042 1101 88-89 -0.9010642275471845 1101 90-91 -1.1525968752953375 1101 92-93 -0.981454156683224 1101 94-95 -1.0483701782554817 1101 96-97 -0.9203361180768113 1101 98-99 -0.9076388571965595 1101 100-101 -0.9415561313125629 1102 1 0.037010101682177776 1102 2 0.16075060365989913 1102 3 0.27862719982658746 1102 4 0.28253002664731497 1102 5 0.2677319773750497 1102 6 0.1925932530756782 1102 7 0.16583507251449703 1102 8 0.07179242735922031 1102 9 0.22075948632286924 1102 10-11 0.3146236765532606 1102 12-13 0.3196565690735582 1102 14-15 0.5585421991530097 1102 16-17 0.35659652533966835 1102 18-19 0.30832122711942134 1102 20-21 0.1676549753476948 1102 22-23 0.27784985312074895 1102 24-25 0.24842554688106588 1102 26-27 0.2844979248582078 1102 28-29 0.3638405410477219 1102 30-31 0.12750339023187252 1102 32-33 0.284990430017082 1102 34-35 0.42555466612066795 1102 36-37 0.3241935315311437 1102 38-39 0.13503741097165545 1102 40-41 0.2208722530069025 1102 42-43 0.29331280343878774 1102 44-45 0.050514005143107 1102 46-47 0.4299810579104104 1102 48-49 0.607551461508379 1102 50-51 0.44246489098329533 1102 52-53 0.3394011524510461 1102 54-55 0.4309125036354189 1102 56-57 0.5240300426245668 1102 58-59 0.3593543399437067 1102 60-61 0.29931384712545395 1102 62-63 0.16544305043603913 1102 64-65 0.40572693757560074 1102 66-67 0.2628031879821364 1102 68-69 0.24815795964183707 1102 70-71 0.2066529714032157 1102 72-73 0.2679145274641215 1102 74-75 0.020654053392554772 1102 76-77 0.3562097370229367 1102 78-79 0.20310901384399216 1102 80-81 0.34997343763288313 1102 82-83 0.4091117280173151 1102 84-85 0.4283643184483594 1102 86-87 0.3268277471065595 1102 88-89 0.35808238136777604 1102 90-91 0.3121558523761365 1102 92-93 0.517907102157718 1102 94-95 0.1752330894675289 1102 96-97 -0.01820880846553763 1102 98-99 -0.06993119778540802 1102 100-101 -0.03450530615767278 1103 1 -0.05984008083061099 1103 2 0.14294481296740003 1103 3 0.17906680052261947 1103 4 0.13710331305502166 1103 5 0.10485534032037691 1103 6 0.04895765065263902 1103 7 0.10399332876507117 1103 8 0.31195243677923656 1103 9 0.2103438281647385 1103 10-11 0.09139941478150604 1103 12-13 0.2213767030471061 1103 14-15 0.39728626258111177 1103 16-17 0.33771855378288507 1103 18-19 0.3164276732879614 1103 20-21 -0.019227326799274636 1103 22-23 0.13762311859508003 1103 24-25 0.22213291150105618 1103 26-27 0.3124982650254182 1103 28-29 0.189225191656746 1103 30-31 0.2626076645636388 1103 32-33 0.15802203940047121 1103 34-35 0.053348289293900564 1103 36-37 -0.021842539275972683 1103 38-39 -0.05312051819506536 1103 40-41 0.12454834841071971 1103 42-43 0.20206785688429107 1103 44-45 -0.1188588442805596 1103 46-47 0.009917407392968869 1103 48-49 0.2321334483465236 1103 50-51 0.23736728916210126 1103 52-53 0.21677807079783662 1103 54-55 0.1738811820858217 1103 56-57 0.10167778640015968 1103 58-59 0.2550574393902849 1103 60-61 0.18580266739942175 1103 62-63 0.14969559841924251 1103 64-65 0.16964402836098458 1103 66-67 0.10895439230487369 1103 68-69 0.056785123301516194 1103 70-71 -0.0333291044017372 1103 72-73 0.03026881160840844 1103 74-75 -0.21489636331227047 1103 76-77 -0.13700261889661647 1103 78-79 -0.13677330138634858 1103 80-81 -0.06799785748833642 1103 82-83 -0.04157514807023688 1103 84-85 -0.13017616799074716 1103 86-87 -0.10511166388083737 1103 88-89 -0.08177867341222722 1103 90-91 -0.05787737934314663 1103 92-93 -0.4755489312309997 1103 94-95 -0.045664297786668584 1103 96-97 -0.014878113608602916 1103 98-99 0.014055431026754661 1103 100-101 0.047314392140183514 1104 1 -0.08027838522190933 1104 2 0.006718193635400382 1104 3 0.08079414913881777 1104 4 0.02669182552929783 1104 5 0.006030451089010569 1104 6 0.12235269029695672 1104 7 0.046423603385676415 1104 8 0.01198148583544878 1104 9 -0.02125420628438235 1104 10-11 0.15025586046130712 1104 12-13 0.07598311891339193 1104 14-15 0.12723920311004377 1104 16-17 0.1315855335678009 1104 18-19 0.05004599315169855 1104 20-21 0.055176173764884595 1104 22-23 0.11663196479715054 1104 24-25 0.07831531511170198 1104 26-27 0.13853661589521238 1104 28-29 0.07296842246219626 1104 30-31 0.07224461251690428 1104 32-33 0.06933343610966602 1104 34-35 0.11399446261585666 1104 36-37 0.2646887893667085 1104 38-39 0.09968944287920323 1104 40-41 0.2816646027336773 1104 42-43 0.14343371292940077 1104 44-45 0.07691467093044224 1104 46-47 0.09662731128645419 1104 48-49 0.08254518049587745 1104 50-51 0.12135911873281202 1104 52-53 0.15285822173061803 1104 54-55 2.815759298684384E-4 1104 56-57 -0.012139167852161847 1104 58-59 0.08561368294979843 1104 60-61 0.1691963921917221 1104 62-63 0.05145994635966389 1104 64-65 -0.002226394501533946 1104 66-67 0.005989859673611875 1104 68-69 -0.008214127538614946 1104 70-71 0.023690217807335046 1104 72-73 0.13426871375895644 1104 74-75 0.22755531587607436 1104 76-77 0.2439240765744728 1104 78-79 0.06890104952014298 1104 80-81 -0.0590021292284888 1104 82-83 -0.0702080383290209 1104 84-85 -0.053717523144506174 1104 86-87 -0.05145408192427681 1104 88-89 0.1945876260132131 1104 90-91 0.19996754762141222 1104 92-93 -0.0700537671344108 1104 94-95 0.05606029881367647 1104 96-97 -0.08624002509765916 1104 98-99 -0.32292095973503265 1104 100-101 -0.04166409015365602 1105 1 -0.029108495389380096 1105 2 0.2599601186669531 1105 3 0.26889572149804053 1105 4 0.27113001691827066 1105 5 0.3427745419515915 1105 6 0.4080673008452962 1105 7 0.4026522150936671 1105 8 0.39111674510986205 1105 9 0.3968114394288662 1105 10-11 0.3990450415384217 1105 12-13 0.40818237536824853 1105 14-15 0.5053012106819281 1105 16-17 0.5344082196530451 1105 18-19 0.42689519219214844 1105 20-21 0.42362496124058424 1105 22-23 0.3029909242887143 1105 24-25 0.39978518106892835 1105 26-27 0.28697761547454803 1105 28-29 0.257161550922703 1105 30-31 0.3072998099429256 1105 32-33 0.49208290459995396 1105 34-35 0.4893091049979361 1105 36-37 0.46920861155255267 1105 38-39 0.45455630964364246 1105 40-41 0.5182867590145861 1105 42-43 0.5670577564961476 1105 44-45 0.7536163547077379 1105 46-47 0.824583869604723 1105 48-49 0.8274497929769851 1105 50-51 0.6636596178403309 1105 52-53 0.7070776130240901 1105 54-55 0.7400188907541576 1105 56-57 0.8143581547031715 1105 58-59 0.7339822039316459 1105 60-61 0.7548399452915291 1105 62-63 0.7695560046608847 1105 64-65 0.7728719490072251 1105 66-67 0.6832378331151787 1105 68-69 0.7442882315686816 1105 70-71 0.617592067574833 1105 72-73 0.4963209097183494 1105 74-75 0.6729780792718216 1105 76-77 0.8556843685353215 1105 78-79 0.8627127984429066 1105 80-81 0.8550896997333837 1105 82-83 0.8826535872380177 1105 84-85 0.8382879454367327 1105 86-87 0.788394366749511 1105 88-89 0.7542321113867523 1105 90-91 0.7786514499827888 1105 92-93 0.766986491173629 1105 94-95 0.673346870661284 1105 96-97 0.5189099345417105 1105 98-99 0.6998199818614275 1105 100-101 0.6569696172260855 1106 1 -0.033560083883330094 1106 2 0.1149686643970611 1106 3 0.09342504644035188 1106 4 0.1368702179243968 1106 5 0.06612605191721599 1106 6 0.08019062331957372 1106 7 0.15633501782917847 1106 8 0.26638648002725773 1106 9 0.22923814082103888 1106 10-11 0.19668446232048353 1106 12-13 0.1947343437457505 1106 14-15 0.20549896129618617 1106 16-17 0.22500907136647896 1106 18-19 0.059737576813787996 1106 20-21 0.12280481311459113 1106 22-23 0.2069369729058934 1106 24-25 0.31631281006326617 1106 26-27 0.22776433956735076 1106 28-29 0.2666237198444037 1106 30-31 0.23160630799731763 1106 32-33 0.2277473598619011 1106 34-35 0.18469562056171895 1106 36-37 0.22330550275451344 1106 38-39 0.28479406131374674 1106 40-41 0.22031051328470141 1106 42-43 0.20754647987185137 1106 44-45 0.1809715997183261 1106 46-47 0.20859758016732854 1106 48-49 0.2981878163067151 1106 50-51 0.28096791082799655 1106 52-53 0.282098839869775 1106 54-55 0.3122663297847197 1106 56-57 0.2395151013809027 1106 58-59 0.29685601088392843 1106 60-61 0.0658500791856298 1106 62-63 0.03443981792622708 1106 64-65 0.18361553750997217 1106 66-67 0.2695948004698323 1106 68-69 0.24708126652368634 1106 70-71 0.13484932814591133 1106 72-73 0.134777960000676 1106 74-75 0.245991279510271 1106 76-77 0.3009641999523751 1106 78-79 0.37943361372975204 1106 80-81 0.3806105823302346 1106 82-83 0.2768212639212635 1106 84-85 0.43954412376863417 1106 86-87 0.4410225362035298 1106 88-89 0.44934366780946533 1106 90-91 0.45613317619827853 1106 92-93 0.5773169260710027 1106 94-95 0.6877858000363801 1106 96-97 0.7008055798488186 1106 98-99 0.5348343237302267 1106 100-101 0.5187572805793117 1107 1 0.06120424424987192 1107 2 0.27804529092143326 1107 3 0.333433399337153 1107 4 0.2581924743488102 1107 5 0.1840898964866966 1107 6 0.2894277135130139 1107 7 0.30935663660154233 1107 8 0.2937130652035975 1107 9 0.37173177798405277 1107 10-11 0.36245674236385383 1107 12-13 0.3789064967108473 1107 14-15 0.499835649670068 1107 16-17 0.4153905679152601 1107 18-19 0.4218017418156279 1107 20-21 0.3616271617540363 1107 22-23 0.3702715040430391 1107 24-25 0.4167444953851316 1107 26-27 0.5250420924239378 1107 28-29 0.40364989820373154 1107 30-31 0.3933948987969913 1107 32-33 0.4997412391637326 1107 34-35 0.6130701254265105 1107 36-37 0.5027366728620919 1107 38-39 0.5668723976832339 1107 40-41 0.32963142991398797 1107 42-43 0.4121882869532456 1107 44-45 0.5965320826633089 1107 46-47 0.5685497235420698 1107 48-49 0.46018612192992947 1107 50-51 0.4213751085976938 1107 52-53 0.6522856615687473 1107 54-55 0.6596793115190067 1107 56-57 0.6387999292124675 1107 58-59 0.5467349414751652 1107 60-61 0.5659375347290663 1107 62-63 0.6772743532279293 1107 64-65 0.6412537380913435 1107 66-67 0.6366519630788403 1107 68-69 0.5787055479901326 1107 70-71 0.4341534318931721 1107 72-73 0.4658929765339366 1107 74-75 0.43624426995440757 1107 76-77 0.4322379453222851 1107 78-79 0.49011990772078917 1107 80-81 0.576099006666908 1107 82-83 0.48162310869778935 1107 84-85 0.5059062230348346 1107 86-87 0.5389313968184304 1107 88-89 0.4986462842175534 1107 90-91 0.6015803714930499 1107 92-93 0.7751348210220179 1107 94-95 0.7481720679692252 1107 96-97 0.8383505421120461 1107 98-99 0.9688381099008261 1107 100-101 1.092556373183431 1108 1 0.013151522294670315 1108 2 0.10472976007827839 1108 3 0.20026700168672562 1108 4 0.24468210928948508 1108 5 0.2507305016220158 1108 6 0.27479498508350275 1108 7 0.3161473830403949 1108 8 0.2255927774215678 1108 9 0.3899780236113699 1108 10-11 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-0.6696286927667003 2316 56-57 -0.5660450905772691 2316 58-59 -0.5558423322611006 2316 60-61 -0.5961339647632684 2316 62-63 -0.5319851924455321 2316 64-65 -0.5970825776597479 2316 66-67 -0.5648477536382188 2316 68-69 -0.48241585177218127 2316 70-71 -0.44210381145529 2316 72-73 -0.5059470162858304 2316 74-75 -0.4685103557314605 2316 76-77 -0.45907716868407533 2316 78-79 -0.30557038509039813 2316 80-81 -0.3742541554037899 2316 82-83 -0.3729189145434333 2316 84-85 -0.41760177339747884 2316 86-87 -0.37037378665836584 2316 88-89 -0.3897540606904464 2316 90-91 -0.4500226012548936 2316 92-93 -0.43425134050615455 2316 94-95 -0.4563756449717964 2316 96-97 -0.5938795237041568 2316 98-99 -0.46867856853256384 2316 100-101 -0.40804765656648456 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8645.0 3 1133.0 4 936.0 5 924.0 6 1069.0 7 1269.0 8 1604.0 9 1783.0 10 2030.0 11 2503.0 12 3206.0 13 3977.0 14 4770.0 15 5701.0 16 6779.0 17 9211.0 18 8554.0 19 5308.0 20 4907.0 21 4865.0 22 5130.0 23 5637.0 24 6346.0 25 7291.0 26 8410.0 27 9940.0 28 11832.0 29 14618.0 30 18297.0 31 22970.0 32 29791.0 33 39468.0 34 56458.0 35 90576.0 36 159677.0 37 309215.0 38 432566.0 39 147562.0 40 115.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.4164115749254 20.220470836957837 14.409953532928856 22.953164055187905 2 16.0593441095321 22.06367853920498 38.929000830685396 22.94797652057753 3 18.60756714796053 26.37009091616045 31.11960178845597 23.902740147423053 4 13.41833795703979 18.739534720468214 37.88298707725089 29.959140245241116 5 13.597558458002451 37.441473182950496 34.79348738628387 14.16748097276318 6 29.370128038418407 37.058762822762084 18.771835676002947 14.79927346281657 7 25.2390450719595 33.54970149038075 23.647383663126202 17.563869774533554 8 24.296451729416418 33.82494281144115 22.11079960784988 19.767805851292547 9 25.56928567890499 16.653480794949836 22.20441174042499 35.57282178572018 10-11 23.72442802701996 26.584912020031094 29.228369584657504 20.46229036829144 12-13 24.95002523248458 25.170048370002135 28.57875579598943 21.301170601523857 14-15 22.533947923178793 26.8286565303153 27.03137897019615 23.606016576309756 16-17 21.40876349693389 29.177292220597685 27.788397118773016 21.625547163695412 18-19 21.219258410001647 28.11758277528162 29.784856181969705 20.87830263274703 20-21 22.42244436080033 27.26780100010528 29.34329126432052 20.96646337477387 22-23 22.14693545590409 27.228293328709473 29.131162046937657 21.493609168448778 24-25 22.092252887417548 27.44401973653433 29.145786723245465 21.31794065280266 26-27 21.678351748187037 27.460669704638796 29.46105979979931 21.399918747374855 28-29 21.618187972935594 27.59546091912741 29.457067527531578 21.329283580405413 30-31 22.03990513029106 27.216117531117302 29.17652886250944 21.567448476082205 32-33 21.804740424179425 27.463068844365957 29.045434626024107 21.68675610543051 34-35 22.211132968523263 27.534697208329117 28.906966551278796 21.34720327186882 36-37 22.079110125480923 27.33435696059081 29.003972604954765 21.582560308973505 38-39 21.82888562172218 27.493999046452096 29.185927394137952 21.491187937687776 40-41 21.963223486771767 27.485479696342992 29.006826365926784 21.544470450958457 42-43 21.98093788783982 27.45809610034371 28.663753367584775 21.897212644231693 44-45 22.121548862092098 27.539125797739665 28.49768633994461 21.841639000223626 46-47 22.27012339511862 27.580100864250923 28.381152511164803 21.768623229465657 48-49 22.000094966369517 27.70788344736179 28.407641076670892 21.884380509597797 50-51 21.835315439788182 27.94512074289772 28.322906746960097 21.896657070354 52-53 22.1720923224889 28.04843566506164 27.949712326382798 21.829759686066662 54-55 22.086795251204876 27.824911224937644 28.020484647864652 22.067808875992828 56-57 22.178496787677066 27.9280990100713 27.90594387250475 21.987460329746884 58-59 22.295079034850186 27.81833404882989 28.05162720427977 21.834959712040156 60-61 22.12193125994764 28.006530225857617 28.20119177309873 21.670346741096015 62-63 21.90337808296552 28.02928591502833 28.197118535098824 21.87021746690732 64-65 22.049130185564543 28.028627048094567 27.97020590528343 21.952036861057458 66-67 21.916929763812902 27.973135364556832 27.899306469385948 22.210628402244314 68-69 21.96002876222653 27.957847512033002 27.948283039575582 22.133840686164888 70-71 22.15914739591593 27.78679256252165 27.94599706290401 22.10806297865841 72-73 22.003528154715916 27.711460517072815 28.077898738932376 22.207112589278896 74-75 21.844591236394468 28.28805464742129 27.74973214577461 22.117621970409633 76-77 21.899544975081316 27.9568190778813 27.815660205858695 22.327975741178687 78-79 21.94115095107541 27.970379718005944 27.90353316541993 22.184936165498716 80-81 22.02717435992179 28.07942649873376 27.676348990259193 22.217050151085257 82-83 22.051048266536842 27.98127622386645 27.825623222712125 22.142052286884585 84-85 22.009548802236996 28.028087707516843 27.730607908758643 22.231755581487516 86-87 21.890265511083893 28.185255653765562 27.80888844543381 22.115590389716736 88-89 22.016059841520697 28.038603615287606 27.52592300055248 22.419413542639216 90-91 22.03081591473243 27.83980678555639 27.75964481020018 22.369732489510994 92-93 22.20879511476564 27.883541099852145 27.778077899627245 22.129585885754967 94-95 21.994333435619545 27.798364980136753 27.897750024424845 22.309551559818857 96-97 21.76489303704621 27.863612989992486 28.0800326074058 22.291461365555502 98-99 21.934795260889125 27.98110354525229 27.66509631908509 22.419004874773492 100-101 21.82277170756148 27.89453653003862 27.79663536582344 22.486056396576455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3640.0 1 2094.5 2 529.0 3 488.5 4 390.0 5 355.5 6 505.0 7 893.0 8 1359.5 9 1543.5 10 1292.0 11 975.5 12 891.0 13 934.5 14 1070.0 15 1177.0 16 1217.0 17 1304.0 18 1489.5 19 1654.0 20 1804.0 21 2039.5 22 2419.0 23 2926.0 24 3493.5 25 4401.0 26 5933.5 27 8303.5 28 11487.0 29 15777.0 30 21157.5 31 26283.0 32 31689.5 33 36856.0 34 40763.0 35 43688.0 36 46495.0 37 48516.0 38 48456.0 39 47331.0 40 45737.5 41 45257.0 42 45716.5 43 46601.5 44 47782.0 45 49112.0 46 50122.5 47 52812.0 48 57811.5 49 56994.0 50 51475.5 51 47618.0 52 45526.0 53 44474.0 54 42245.0 55 38197.5 56 34037.0 57 30289.5 58 27624.5 59 25338.0 60 22071.0 61 18774.5 62 15213.5 63 11208.5 64 7997.0 65 5820.5 66 4314.5 67 3379.5 68 2571.5 69 1925.0 70 1451.0 71 1081.5 72 778.0 73 526.0 74 385.5 75 282.5 76 169.5 77 112.0 78 98.0 79 77.0 80 53.0 81 40.5 82 35.5 83 30.0 84 26.0 85 19.0 86 15.5 87 10.5 88 5.5 89 5.5 90 5.0 91 4.0 92 3.5 93 2.0 94 1.5 95 1.0 96 0.5 97 2.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13751887362352266 2 0.141298752708627 3 0.13580074676665707 4 0.14116130256007775 5 0.13009656560186328 6 0.11016629406222231 7 0.1158704752270161 8 0.106386414977118 9 0.10288143618911215 10-11 0.09161052400807382 12-13 0.10923850555951489 14-15 0.12745065024229024 16-17 0.13824048690340623 18-19 0.13415134498406608 20-21 0.12549198562546346 22-23 0.11965035431212043 24-25 0.10480573826880163 26-27 0.10868870496531788 28-29 0.11346509762740425 30-31 0.10312197394907335 32-33 0.1024003606691898 34-35 0.11779477730670558 36-37 0.1198908920720816 38-39 0.10679876542276573 40-41 0.10934159317092683 42-43 0.10370613708040764 44-45 0.12013142983204278 46-47 0.11913491625506074 48-49 0.1324332181272005 50-51 0.11937545401502192 52-53 0.10418721260033001 54-55 0.096146378910199 56-57 0.11583611268987878 58-59 0.10563043916009711 60-61 0.10477137573166433 62-63 0.10593970199433292 64-65 0.1259043360711112 66-67 0.11769168969529364 68-69 0.12219318206028151 70-71 0.10971958107943725 72-73 0.11147207047344017 74-75 0.0955622157788647 76-77 0.11999397968349354 78-79 0.1198908920720816 80-81 0.12057814281482784 82-83 0.1264884992024455 84-85 0.11662645104403696 86-87 0.12631668651675895 88-89 0.11229677136473566 90-91 0.1213341186318487 92-93 0.13466678304112578 94-95 0.11236549643901027 96-97 0.09728034263573031 98-99 0.10081968396087343 100-101 0.1427419792683941 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1455073.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.984014506209085 #Duplication Level Percentage of deduplicated Percentage of total 1 60.67650228223221 29.11502165696659 2 19.534922792769503 18.747280373318524 3 8.59906967021615 12.37853651386663 4 4.243258982983193 8.1443440237227 5 2.2711226278836745 5.448879056087497 6 1.347664555178677 3.8799813339118474 7 0.8599581232440743 2.88849701423332 8 0.5478304644416369 2.102968396216861 9 0.3959056325053729 1.7097427451904927 >10 1.4613301238629635 11.195632813295553 >50 0.045302611570407776 1.4715947613425264 >100 0.014828723762443222 1.2671485372719287 >500 0.0010077415904712307 0.3073336601266973 >1k 0.0012956677591772965 1.3430391144489207 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4433 0.3046582542594083 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4049 0.27826782573795267 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2283 0.15689934456896665 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1906 0.13098999156743338 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1895 0.13023401575041252 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1463 0.10054478366377494 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.436253713731201E-4 0.0 18-19 0.0 0.0 0.0 3.436253713731201E-4 0.0 20-21 0.0 0.0 0.0 4.1235044564774415E-4 0.0 22-23 0.0 0.0 0.0 5.154380570596801E-4 0.0 24-25 0.0 0.0 0.0 9.965135769820484E-4 0.0 26-27 0.0 0.0 0.0 0.0015119516340417285 0.0 28-29 0.0 0.0 0.0 0.003298803565181953 0.0 30-31 0.0 1.3745014854924805E-4 0.0 0.007491033095934018 0.0 32-33 0.0 1.7181268568656008E-4 0.0 0.016975093345832134 0.0 34-35 0.0 2.0617522282387208E-4 0.0 0.03264441028044641 0.0 36-37 0.0 2.0617522282387208E-4 0.0 0.05250595674581275 0.0 38-39 0.0 2.0617522282387208E-4 0.0 0.07769369646746246 0.0 40-41 0.0 2.0617522282387208E-4 0.0 0.1018505600749928 0.0 42-43 0.0 2.0617522282387208E-4 0.0 0.13129925440166917 0.0 44-45 0.0 2.0617522282387208E-4 0.0 0.1667613927273752 0.0 46-47 0.0 2.0617522282387208E-4 0.0 0.20607213521246015 0.0 48-49 0.0 2.749002970984961E-4 0.0 0.24589831575460475 0.0 50-51 0.0 4.1235044564774415E-4 0.0 0.2842125446627076 0.0 52-53 0.0 4.1235044564774415E-4 0.0 0.3247259759475985 0.0 54-55 0.0 4.1235044564774415E-4 0.0 0.3700157998945758 0.0 56-57 0.0 4.1235044564774415E-4 0.0 0.4268514363196898 0.0 58-59 0.0 4.467129827850562E-4 0.0 0.48207203349935024 0.0 60-61 0.0 4.8107551992236815E-4 0.0 0.542515736323882 0.0 62-63 0.0 4.8107551992236815E-4 0.0 0.6073922064391271 0.0 64-65 0.0 4.8107551992236815E-4 0.0 0.6820619996385061 0.0 66-67 0.0 4.8107551992236815E-4 0.0 0.7774867652688215 0.0 68-69 0.0 4.8107551992236815E-4 0.0 0.8803682014579337 0.0 70-71 0.0 4.8107551992236815E-4 0.0 0.9831809125727713 0.0 72-73 0.0 4.8107551992236815E-4 0.0 1.093725194543504 0.0 74-75 0.0 4.8107551992236815E-4 0.0 1.2135473615413108 0.0 76-77 0.0 5.154380570596801E-4 0.0 1.342750501177604 0.0 78-79 0.0 5.498005941969922E-4 0.0 1.4844272417947415 0.0 80-81 0.0 5.498005941969922E-4 0.0 1.637890332649977 0.0 82-83 0.0 5.498005941969922E-4 0.0 1.8037926619489193 0.0 84-85 0.0 5.841631313343043E-4 0.0 1.9863951842965955 0.0 86-87 0.0 6.872507427462403E-4 0.0 2.2089613373349652 0.0 88-89 0.0 7.559758170208643E-4 0.0 2.470563332561322 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6240 0.0 27.56309 1 GTATCAA 8470 0.0 24.120647 1 GAGTACT 4130 0.0 22.089834 12-13 TCAACGC 9125 0.0 22.023254 4 ATCAACG 9345 0.0 21.504784 3 CAACGCA 9355 0.0 21.481796 5 TATCAAC 9450 0.0 21.317583 2 GTACATG 5000 0.0 21.285406 1 AACGCAG 9450 0.0 21.207537 6 TACATGG 5110 0.0 19.990398 2 GTACTTT 4545 0.0 19.654985 14-15 ACATGGG 5115 0.0 19.505075 3 ACGCAGA 10415 0.0 19.06082 7 CGCAGAG 10415 0.0 18.836708 8 AGAGTAC 7000 0.0 18.393324 10-11 CATGGGG 2800 0.0 17.47645 4 GCAGAGT 11100 0.0 16.86116 9 GTGTAGC 870 0.0 16.38347 1 AGTACTT 4560 0.0 16.35973 12-13 ACCGTAT 175 0.00216942 16.286488 8 >>END_MODULE