FastQCFastQC Report
Fri 10 Feb 2017
SRR2031764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455073
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58090.39922395646129094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53230.3658235703638237No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT37680.2589560798667833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25900.17799794237127622No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23660.16260352573376044No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16340.11229677136473566No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT16210.11140334539916555TruSeq Adapter, Index 1 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15490.1064551400513926No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15380.10569916423437174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66200.030.3613361
GTATCAA87250.023.635391
GAGTACT54350.023.02716312-13
GTACTTT59150.020.99792314-15
TCAACGC99500.020.6749084
GTACATG45750.020.4603771
ATCAACG100550.020.3659113
CAACGCA101400.020.281935
TATCAAC101900.020.1427232
AACGCAG102750.020.061686
TACCGTC1456.150379E-419.6536247
TACATGG47250.019.2061442
AGAGTAC78000.018.29823710-11
ACATGGG47500.018.2048193
ACGCAGA113450.018.043977
ATACCGT1851.5226075E-417.9715566
CGCAGAG114200.017.8006978
ACTTTTT73350.017.15951716-17
AGTACTT58200.016.68897612-13
GCAGAGT118950.016.4909259
Produced by FastQC (version 0.11.5)