FastQCFastQC Report
Fri 10 Feb 2017
SRR2031761_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031761_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037119
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49820.24456106884281184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48640.2387685746389877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21430.10519758541351781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37850.058.9868281
GTATCAA63750.037.779031
GTGGTAT16350.034.8647461
TCAACGC73000.032.0793654
ATCAACG74300.031.4541513
CAACGCA75200.031.0777055
TATCAAC76800.030.7410142
TGGTATC17850.030.6043152
AACGCAG79000.029.5799226
ACGCAGA86700.026.6789597
CGCAGAG87650.026.1207798
GAGTACT57000.022.79433312-13
GCAGAGT98200.022.4922289
CAGAGTA93650.021.9384910-11
GTACTTT62850.020.21964614-15
GTACATG55850.019.6477051
GCGTCAG4750.019.0012871
AGAGTAC86250.018.3130610-11
ACATGGG57750.018.260083
TACATGG58450.018.1235562
Produced by FastQC (version 0.11.5)