##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031761_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2037119
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02786238800973	33.0	31.0	34.0	30.0	34.0
2	32.327699069126545	34.0	31.0	34.0	31.0	34.0
3	32.428489450051764	34.0	31.0	34.0	31.0	34.0
4	35.85897141993178	37.0	35.0	37.0	35.0	37.0
5	35.85940732966508	37.0	37.0	37.0	35.0	37.0
6	35.89069857970988	37.0	37.0	37.0	35.0	37.0
7	35.875732836422415	37.0	37.0	37.0	35.0	37.0
8	35.86750749465299	37.0	37.0	37.0	35.0	37.0
9	37.644074302973955	39.0	38.0	39.0	35.0	39.0
10-11	37.57587799240005	39.0	38.0	39.0	35.0	39.0
12-13	37.526550486250436	39.0	38.0	39.0	35.0	39.0
14-15	38.926017085894344	41.0	39.0	41.0	36.0	41.0
16-17	38.85009491345376	41.0	39.0	41.0	36.0	41.0
18-19	38.86076881124765	41.0	39.0	41.0	36.0	41.0
20-21	38.870313663561134	41.0	39.0	41.0	35.0	41.0
22-23	38.77360036404353	41.0	39.0	41.0	35.0	41.0
24-25	38.703747547394144	41.0	39.0	41.0	35.0	41.0
26-27	38.58741315553976	40.5	38.5	41.0	35.0	41.0
28-29	38.480954720858236	40.0	38.0	41.0	34.0	41.0
30-31	38.32068033335314	40.0	38.0	41.0	34.0	41.0
32-33	38.1125336811448	40.0	38.0	41.0	34.0	41.0
34-35	37.9487619525418	40.0	38.0	41.0	33.5	41.0
36-37	37.784273034614074	40.0	38.0	41.0	33.0	41.0
38-39	37.59765850694044	40.0	38.0	41.0	33.0	41.0
40-41	37.50113297259512	40.0	37.5	41.0	33.0	41.0
42-43	37.47338398002277	40.0	38.0	41.0	33.0	41.0
44-45	37.32942847226893	40.0	37.5	41.0	32.0	41.0
46-47	37.234088190233365	40.0	37.5	41.0	32.0	41.0
48-49	37.25941488936091	40.0	38.0	41.0	32.0	41.0
50-51	36.703948321133915	39.5	37.0	40.5	31.0	41.0
52-53	36.71430289541259	39.5	37.0	40.5	31.0	41.0
54-55	36.932471053482885	40.0	37.0	41.0	31.0	41.0
56-57	36.80130763102204	40.0	36.5	41.0	31.0	41.0
58-59	36.604899861029224	40.0	36.0	41.0	31.0	41.0
60-61	36.361499990918546	39.0	35.5	41.0	31.0	41.0
62-63	36.07749645455175	39.0	35.0	41.0	30.0	41.0
64-65	35.80654615660646	39.0	35.0	41.0	30.0	41.0
66-67	35.50908218911119	38.0	35.0	41.0	29.0	41.0
68-69	35.17051507545706	37.0	35.0	40.0	29.0	41.0
70-71	34.81245548247304	37.0	35.0	39.5	29.0	41.0
72-73	34.450035564932634	36.0	35.0	39.0	28.5	41.0
74-75	34.047309214631056	36.0	34.0	39.0	28.0	41.0
76-77	33.67287478051111	35.0	34.0	38.0	27.5	40.0
78-79	33.289537577333476	35.0	34.0	37.0	26.5	39.0
80-81	32.944127220844734	35.0	34.0	37.0	26.0	39.0
82-83	32.62213768562367	35.0	34.0	36.0	26.0	38.5
84-85	32.29954509284926	35.0	34.0	36.0	26.0	37.0
86-87	32.02665234578834	35.0	34.0	35.5	25.0	37.0
88-89	31.76007881719232	35.0	33.0	35.0	25.0	36.5
90-91	31.51824341140601	35.0	33.0	35.0	24.0	36.0
92-93	31.311598635131283	35.0	33.0	35.0	23.0	36.0
94-95	31.06634197609467	35.0	33.0	35.0	20.5	36.0
96-97	30.807977344475212	35.0	33.0	35.0	18.5	35.5
98-99	30.55974663237641	35.0	32.0	35.0	9.0	35.0
100-101	29.972352621520884	34.5	31.5	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.13480912926553756
1101	2	-0.014547149393052905
1101	3	0.03877522859863802
1101	4	0.22520848787838332
1101	5	-0.029692527614813002
1101	6	0.10479427629437055
1101	7	0.11878959300280911
1101	8	0.06516718400793309
1101	9	0.03073122255785421
1101	10-11	0.02843793542881201
1101	12-13	0.059745209984434666
1101	14-15	-0.10997827051257048
1101	16-17	0.08270717789359594
1101	18-19	0.15774824357144723
1101	20-21	0.11304709567318838
1101	22-23	0.12391362166186326
1101	24-25	-0.09427216321441989
1101	26-27	-0.08385953941778723
1101	28-29	-0.21612868597129165
1101	30-31	-0.0745478255202201
1101	32-33	-0.11254471265531407
1101	34-35	-0.014308081481388513
1101	36-37	-0.2593847507607734
1101	38-39	-0.03506069120023625
1101	40-41	-0.2733469225311538
1101	42-43	-0.2704793696007428
1101	44-45	-0.403018690543675
1101	46-47	-0.30371069211359725
1101	48-49	-0.09821423841018628
1101	50-51	-0.08235965857085148
1101	52-53	-0.08058026642064675
1101	54-55	0.08378336573610312
1101	56-57	-0.09910124416394694
1101	58-59	-0.21207785649641409
1101	60-61	-0.4064039069239129
1101	62-63	-0.2669101725623477
1101	64-65	-0.06498986677907226
1101	66-67	-0.380958414662409
1101	68-69	-0.3759373185439969
1101	70-71	-0.4553121829601068
1101	72-73	-0.030979813741971896
1101	74-75	0.054233329706789846
1101	76-77	-0.034815377165415384
1101	78-79	-0.21855796540139494
1101	80-81	-0.4190295426816846
1101	82-83	-0.54222163619675
1101	84-85	-0.5602075997045652
1101	86-87	-0.5509338983313192
1101	88-89	-0.6889504873673253
1101	90-91	-1.0532290704260205
1101	92-93	-0.8944745345947922
1101	94-95	-0.9885566463940876
1101	96-97	-1.0401075986469692
1101	98-99	-1.0264045428814903
1101	100-101	-1.0035785732303282
1102	1	-0.028803893398730906
1102	2	0.0996363194248886
1102	3	0.20945914965614776
1102	4	0.22335599101035797
1102	5	0.1681931821765943
1102	6	0.09102203650066087
1102	7	0.07926919166126112
1102	8	-0.021886124082378444
1102	9	0.1569594321947676
1102	10-11	0.1596527183257308
1102	12-13	0.23306602480216299
1102	14-15	0.33261047799166477
1102	16-17	0.23946085620238478
1102	18-19	0.20543213092166468
1102	20-21	0.10267929288334443
1102	22-23	0.2433083790535875
1102	24-25	0.15945811099444285
1102	26-27	0.1151666005910883
1102	28-29	0.16111074257766234
1102	30-31	-0.023759355662967607
1102	32-33	-0.026915537866123884
1102	34-35	0.1647282268954342
1102	36-37	0.17912471256288143
1102	38-39	-0.011591283381264361
1102	40-41	0.07320482097879477
1102	42-43	0.2731013565667908
1102	44-45	0.013623866042451027
1102	46-47	-0.12169831029980571
1102	48-49	0.06839180193745165
1102	50-51	0.11833499264289316
1102	52-53	0.033837046770706536
1102	54-55	0.1349868566885064
1102	56-57	-0.08139508190825495
1102	58-59	0.04542131486586243
1102	60-61	-0.1825291688576982
1102	62-63	-0.07541480450675664
1102	64-65	0.07119323125860433
1102	66-67	0.057906379751166526
1102	68-69	0.08729855388511254
1102	70-71	0.08227636891822954
1102	72-73	0.07034432124451939
1102	74-75	-0.10791632507430649
1102	76-77	0.09712692155988378
1102	78-79	0.014067327430751675
1102	80-81	-0.004793546659143999
1102	82-83	0.18423803021337193
1102	84-85	0.061385856585495446
1102	86-87	0.06296937857974427
1102	88-89	0.14457830147331308
1102	90-91	0.11098796524191812
1102	92-93	0.11281958404365611
1102	94-95	-0.1742698085318004
1102	96-97	-0.496910292983582
1102	98-99	-0.33578837349108426
1102	100-101	-0.41672048656886673
1103	1	-0.13091949507929357
1103	2	0.017372936552462193
1103	3	0.030173860887096282
1103	4	0.02075388194072758
1103	5	0.0038035895677452913
1103	6	-0.05511699696435812
1103	7	0.028163706826077828
1103	8	0.030273634283872752
1103	9	0.04324812808323486
1103	10-11	0.06576097275185333
1103	12-13	0.06989466028219482
1103	14-15	0.15713925560919506
1103	16-17	0.1212783610622381
1103	18-19	0.01028570839704912
1103	20-21	-0.17142790596282964
1103	22-23	-0.028975723494433225
1103	24-25	0.01569282598786259
1103	26-27	-0.007666063859907979
1103	28-29	-0.06087393061060453
1103	30-31	-0.004279463938303252
1103	32-33	-0.025602594992854222
1103	34-35	-0.2161218807528158
1103	36-37	-0.1866964450216173
1103	38-39	-0.08862053130668812
1103	40-41	0.07803919421514394
1103	42-43	-0.11369357351409803
1103	44-45	-0.20283272976449496
1103	46-47	-4.860084556668198E-5
1103	48-49	0.008567331295111558
1103	50-51	-0.07404118573966656
1103	52-53	-0.04137171767819581
1103	54-55	-0.1400648771799311
1103	56-57	-0.12549363231481436
1103	58-59	-0.036643493562060314
1103	60-61	0.12344661551135516
1103	62-63	-0.03779735817411023
1103	64-65	-0.009866947249363989
1103	66-67	-0.0631815730508265
1103	68-69	-0.1616393341188953
1103	70-71	-0.06888254811946126
1103	72-73	-0.08709320619011152
1103	74-75	-0.21845892974393877
1103	76-77	-0.17836441954920446
1103	78-79	-0.3355053455903061
1103	80-81	-0.3194478180999596
1103	82-83	-0.2894404046019545
1103	84-85	-0.33687674766608566
1103	86-87	-0.20674762670860858
1103	88-89	-0.12274013355103719
1103	90-91	-0.15089188154221844
1103	92-93	-0.5681692644335925
1103	94-95	-0.030982016679626412
1103	96-97	-0.125610701628883
1103	98-99	-0.16312593436983036
1103	100-101	-0.13336324591753979
1104	1	0.051695750035953836
1104	2	0.061503918183440476
1104	3	0.15997817819141602
1104	4	0.06974036842721887
1104	5	0.1297469030110534
1104	6	0.19042968608232513
1104	7	0.20802944919181954
1104	8	0.15550763451787475
1104	9	0.25196902430413104
1104	10-11	0.30143577092665197
1104	12-13	0.24498899162652066
1104	14-15	0.3623830546030007
1104	16-17	0.4505349715688425
1104	18-19	0.45634865446000106
1104	20-21	0.2758708538359329
1104	22-23	0.3253774121087005
1104	24-25	0.2605594458544829
1104	26-27	0.33223390104006256
1104	28-29	0.2273741388874626
1104	30-31	0.3788374191785806
1104	32-33	0.4134476565573948
1104	34-35	0.4049220527911217
1104	36-37	0.41111565279048534
1104	38-39	0.2635534771173269
1104	40-41	0.387105972031911
1104	42-43	0.33860328503276094
1104	44-45	0.4553806784489183
1104	46-47	0.4430929985460281
1104	48-49	0.4160568012845829
1104	50-51	0.454854186905699
1104	52-53	0.5348904210839436
1104	54-55	0.5430476184325599
1104	56-57	0.5208406077694292
1104	58-59	0.5081602387416737
1104	60-61	0.5364935910583313
1104	62-63	0.491229639602885
1104	64-65	0.5576713702889506
1104	66-67	0.4964628928436383
1104	68-69	0.5660063310267702
1104	70-71	0.5685523705654631
1104	72-73	0.4484711731242683
1104	74-75	0.5694673894323756
1104	76-77	0.6564862590514764
1104	78-79	0.4875283211933663
1104	80-81	0.36911980796766386
1104	82-83	0.40710516694361587
1104	84-85	0.5289497119103714
1104	86-87	0.49351635480537226
1104	88-89	0.4958943288334261
1104	90-91	0.5144987525984099
1104	92-93	0.40454777078344506
1104	94-95	0.5341614547138427
1104	96-97	0.4153137455455642
1104	98-99	0.5027107627168661
1104	100-101	0.4002340689297732
1105	1	-0.11720458521776678
1105	2	0.13940158442697026
1105	3	0.18423118082787937
1105	4	0.15544570319270434
1105	5	0.2423905250531746
1105	6	0.14986154495027648
1105	7	0.07673530473977763
1105	8	0.17017091609844925
1105	9	0.18704209659524906
1105	10-11	0.23251956808187657
1105	12-13	0.08425639715333233
1105	14-15	0.2279784554427522
1105	16-17	0.20547019924843113
1105	18-19	0.11181849789976184
1105	20-21	0.17992888853306255
1105	22-23	-0.0051689212571872645
1105	24-25	0.02639041939222153
1105	26-27	0.12247045020517788
1105	28-29	0.029781944114809278
1105	30-31	0.02152164597935524
1105	32-33	0.0821771100612878
1105	34-35	0.07873930696551668
1105	36-37	0.15887791559033815
1105	38-39	0.14859684853287547
1105	40-41	0.24835609409030468
1105	42-43	0.1389218574378006
1105	44-45	0.3284847047484334
1105	46-47	0.40205493260194913
1105	48-49	0.5147247373585344
1105	50-51	0.37861041188373434
1105	52-53	0.503309846270291
1105	54-55	0.507839875048127
1105	56-57	0.48921731251832057
1105	58-59	0.4470252584337615
1105	60-61	0.4326178302276844
1105	62-63	0.5853468319343662
1105	64-65	0.5206388539331215
1105	66-67	0.42315462216694755
1105	68-69	0.530487338740862
1105	70-71	0.3506577964678783
1105	72-73	0.23338190775304923
1105	74-75	0.37388915813986046
1105	76-77	0.5430533260696677
1105	78-79	0.5299924360258998
1105	80-81	0.47423039287147617
1105	82-83	0.5124944518441765
1105	84-85	0.4741617680246861
1105	86-87	0.4810847075053033
1105	88-89	0.3629483274663734
1105	90-91	0.5218405649214297
1105	92-93	0.5517583296481412
1105	94-95	0.5821141286063671
1105	96-97	0.40706056389012346
1105	98-99	0.5604097992692871
1105	100-101	0.4728364352351484
1106	1	0.20025930769041622
1106	2	0.2988858544290167
1106	3	0.2836499609541079
1106	4	0.25293596071372093
1106	5	0.2533940891582418
1106	6	0.2675646723082181
1106	7	0.33393952396552606
1106	8	0.28960838225498975
1106	9	0.4580394690109344
1106	10-11	0.37912418100142276
1106	12-13	0.39172010142581826
1106	14-15	0.3562216024188132
1106	16-17	0.34633140997891587
1106	18-19	0.22648098425141683
1106	20-21	0.3277205193219572
1106	22-23	0.36802794339559597
1106	24-25	0.4593492923715985
1106	26-27	0.3653655421262485
1106	28-29	0.34567156116215614
1106	30-31	0.31846502641982255
1106	32-33	0.35214059240941964
1106	34-35	0.5264908249280751
1106	36-37	0.4475801352776898
1106	38-39	0.5019930678296447
1106	40-41	0.4290554997769007
1106	42-43	0.42191202175059317
1106	44-45	0.4102335906881933
1106	46-47	0.3070860131072237
1106	48-49	0.23580050455100832
1106	50-51	0.2668376579800835
1106	52-53	0.36267721762073535
1106	54-55	0.45195405290717616
1106	56-57	0.32730213866277325
1106	58-59	0.3795322379545851
1106	60-61	0.3060233037698623
1106	62-63	0.3345246022160282
1106	64-65	0.38526139390170044
1106	66-67	0.44137877241860934
1106	68-69	0.4421131787245258
1106	70-71	0.4841244878421236
1106	72-73	0.4315855965796018
1106	74-75	0.48727779872005783
1106	76-77	0.5174046966744612
1106	78-79	0.5965897143456687
1106	80-81	0.6542068799865532
1106	82-83	0.7385568591907585
1106	84-85	0.8091543557513816
1106	86-87	0.5908451623614539
1106	88-89	0.5462927940046178
1106	90-91	0.5985822827046725
1106	92-93	0.7207495614170512
1106	94-95	0.7788608840723654
1106	96-97	0.6985437730937711
1106	98-99	0.8207941944366652
1106	100-101	0.9147460720781382
1107	1	0.13368803311519173
1107	2	0.2572972552811237
1107	3	0.35174836452682
1107	4	0.27572098201652295
1107	5	0.3163175787121659
1107	6	0.31717069869290526
1107	7	0.354321562875235
1107	8	0.36715830269028515
1107	9	0.41171869492336555
1107	10-11	0.40788457574502246
1107	12-13	0.45168718473776437
1107	14-15	0.45876221891694513
1107	16-17	0.5445261253426068
1107	18-19	0.45300779242347744
1107	20-21	0.372167672958831
1107	22-23	0.4396490100759465
1107	24-25	0.4958078930594567
1107	26-27	0.519239829893813
1107	28-29	0.40512211554848676
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2316	94-95	-0.3137317734975831
2316	96-97	-0.31484830578649436
2316	98-99	-0.36112574191781377
2316	100-101	-0.3822972107189564
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9620.0
3	1453.0
4	1201.0
5	1207.0
6	1348.0
7	1652.0
8	1992.0
9	2448.0
10	2750.0
11	3299.0
12	4153.0
13	5234.0
14	5848.0
15	7234.0
16	8441.0
17	11508.0
18	11103.0
19	6903.0
20	6264.0
21	6480.0
22	7043.0
23	7733.0
24	8757.0
25	9907.0
26	11752.0
27	14041.0
28	17124.0
29	20738.0
30	25398.0
31	32381.0
32	41649.0
33	55529.0
34	79179.0
35	126160.0
36	221563.0
37	430988.0
38	612879.0
39	213984.0
40	176.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.36070454030421	19.77716069136618	14.259660982622446	23.602473785707158
2	16.304034702551665	21.797589423272616	38.896874548348684	23.001501325827032
3	18.703012415979046	25.77561772666363	31.02520063274401	24.49616922461331
4	13.762333796075996	19.053232447567847	37.25607693850504	29.928356817851114
5	14.178855043028788	36.69307508852507	34.564639519634774	14.563430348811377
6	29.46865245048318	36.61129195857807	18.98765716038025	14.932398430558505
7	25.370907339512666	32.98249407786744	23.65196536166783	17.994633220952064
8	24.182706223398792	34.06620515726194	22.144146419731126	19.60694219960814
9	25.482322104335186	16.878091831986158	22.060502241033042	35.579083822645615
10-11	23.68360668625505	26.53622212832028	29.32361120665088	20.456559978773793
12-13	24.781202346487813	25.174445947679235	28.50539419943574	21.53895750639721
14-15	22.439195823057478	27.036462230938525	26.64422874879731	23.880113197206686
16-17	21.562992258552594	29.15610923509918	27.671698922902344	21.609199583445882
18-19	21.363467113323644	28.023284821240946	29.5610808738321	21.052167191603306
20-21	22.487091708465528	27.18629963947713	29.236512340597038	21.0900963114603
22-23	22.329774761391867	27.21913121303291	28.75938514678956	21.69170887878567
24-25	21.927495095707418	27.544114062641277	28.990069544637908	21.538321297013393
26-27	21.637711414620338	27.592212550814203	29.265985343492247	21.50409069107321
28-29	21.582457503485013	27.49888255979933	29.43223225850207	21.48642767821359
30-31	22.044197781904472	27.226903929210426	29.199641865386006	21.529256423499096
32-33	21.84990708842926	27.534380229577454	28.861063602226928	21.75464907976636
34-35	22.028381705133604	27.648842010899937	28.84528903897447	21.47748724499199
36-37	21.958975946841242	27.45851886801726	28.947735739632176	21.634769445509324
38-39	21.783287557735488	27.59713247698812	29.07035485793839	21.549225107338003
40-41	21.93120746661215	27.463177045042634	29.005018497477973	21.60059699086724
42-43	21.94429722626202	27.539292512413628	28.74164941204047	21.77476084928388
44-45	22.055128729377348	27.8263008587201	28.437042920277435	21.68152749162512
46-47	21.982004563365535	27.676062481492814	28.416718578942906	21.925214376198742
48-49	21.931602295488897	27.77907762635634	28.50178797448911	21.787532103665654
50-51	21.945658603210784	27.973873940954164	28.23828982881037	21.842177627024682
52-53	22.04091460807776	28.02780876376556	28.108203069332717	21.823073558823964
54-55	22.096265150759482	27.95179077100272	28.028468957004694	21.923475121233103
56-57	22.018326755884075	28.125795560098883	27.97727462611747	21.878603057899575
58-59	21.978269576178107	27.978878932250907	28.184438323582818	21.858413167988168
60-61	22.066974453980556	27.99322343737331	28.23558751913433	21.704214589511807
62-63	21.900531292914316	28.014294268410172	28.261303826178786	21.823870612496723
64-65	21.971909143143343	27.915313576870656	28.15637532003533	21.95640195995067
66-67	21.90204547627069	28.090103280099687	28.109639238426908	21.89821200520271
68-69	21.821422550627563	28.161547183665885	28.15299518876563	21.864035076940922
70-71	21.845440885764447	27.956055782913015	28.173344259596213	22.02515907172633
72-73	21.856430370285004	27.991848928494818	28.130976406073348	22.02074429514683
74-75	21.89776108218865	28.104351799757755	27.965982600662855	22.03190451739074
76-77	21.843145070425653	28.21469004579873	27.914763301155205	22.027401582620417
78-79	21.78492176806387	28.167723766437064	27.990199812744663	22.0571546527544
80-81	21.802346155207193	28.213061915377992	27.935962387597264	22.04862954181755
82-83	21.861062478434782	28.163980209640965	27.941102743341695	22.03385456858256
84-85	22.01778929349533	28.129922391774343	27.819509148000176	22.032779166730148
86-87	21.80034523910167	28.237206394394388	27.896956155027297	22.065492211476645
88-89	21.876882090204692	28.111507463489755	27.899865613691333	22.11174483261422
90-91	21.913850035866535	28.146959650825348	27.95822722253937	21.98096309076875
92-93	21.927796243918216	28.091349883058705	27.930070184441842	22.05078368858124
94-95	21.84834365452123	28.120547805176994	27.940211646192203	22.090896894109576
96-97	21.8857641813252	27.991613213267513	27.96532465630783	22.15729794909946
98-99	21.712559940777272	28.1450956853451	27.91278947061947	22.22955490325816
100-101	21.823444582446513	28.10504041292142	27.909974911371293	22.161540093260776
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3920.0
1	2250.5
2	573.5
3	511.5
4	397.0
5	384.0
6	547.5
7	966.5
8	1542.0
9	1773.5
10	1509.0
11	1232.0
12	1254.5
13	1311.0
14	1369.5
15	1504.0
16	1635.5
17	1762.0
18	1898.5
19	2063.0
20	2327.5
21	2670.0
22	3110.0
23	3712.5
24	4479.0
25	5759.0
26	7794.0
27	10765.5
28	14842.5
29	20784.5
30	28675.0
31	37622.5
32	46520.0
33	54455.0
34	61344.5
35	66035.0
36	68839.5
37	70436.0
38	69198.5
39	66686.5
40	65579.5
41	65730.5
42	66177.0
43	67606.0
44	68987.0
45	69870.5
46	71065.0
47	72155.0
48	73061.0
49	72237.5
50	69921.5
51	66559.5
52	63556.5
53	61431.0
54	58633.5
55	55289.5
56	50249.0
57	43788.5
58	39166.0
59	35152.5
60	29622.5
61	24179.0
62	19168.5
63	14483.5
64	10498.5
65	7495.5
66	5660.0
67	4402.5
68	3295.5
69	2477.5
70	1903.0
71	1393.0
72	1010.0
73	757.5
74	552.0
75	418.5
76	307.5
77	194.0
78	129.5
79	93.5
80	73.5
81	57.0
82	44.0
83	32.0
84	22.0
85	22.5
86	19.5
87	18.0
88	15.5
89	11.0
90	6.0
91	5.5
92	8.5
93	7.0
94	5.0
95	4.0
96	3.5
97	1.5
98	1.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13882350515605618
2	0.14319242027588963
3	0.13769445967564978
4	0.14093432931507685
5	0.13092018679321138
6	0.11069554601375767
7	0.11678257382116605
8	0.10809383251542987
9	0.10406853993311141
10-11	0.09321988553442387
12-13	0.11035192347624267
14-15	0.12844119562971038
16-17	0.13791535987833797
18-19	0.13482275704070307
20-21	0.12635491593765508
22-23	0.12048878833293489
24-25	0.10637571982785492
26-27	0.11069554601375767
28-29	0.11459811626124934
30-31	0.10455942927241856
32-33	0.10404399546614605
34-35	0.119187931583771
36-37	0.1207096885356231
38-39	0.10760294317612275
40-41	0.11030283454231196
42-43	0.10590937495551316
44-45	0.12090604427134595
46-47	0.11960518752218206
48-49	0.13224558800934066
50-51	0.12004698792755848
52-53	0.10549211901710209
54-55	0.09768697852211873
56-57	0.1172734631604732
58-59	0.10721023170467704
60-61	0.10622845302606279
62-63	0.10689115363412742
64-65	0.12655127167337793
66-67	0.11827978630605282
68-69	0.1230168684303666
70-71	0.11126006875396086
72-73	0.11263455890402083
74-75	0.09739244491853445
76-77	0.1205378772668656
78-79	0.12095513320527666
80-81	0.12129875574279166
82-83	0.12728760568233863
84-85	0.11840250864087959
86-87	0.12699307207875438
88-89	0.11356724864870436
90-91	0.12235416782230199
92-93	0.13573090231842128
94-95	0.11329725951208545
96-97	0.09881602400252512
98-99	0.10257132744822467
100-101	0.14385512088395425
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2037119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.76059173871286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.1870991976156	30.447062628486755
2	19.640578572833743	19.546536237497833
3	8.46148380430254	12.63145323268889
4	4.190260277271646	8.340393237450405
5	2.1665035565561173	5.390324948912919
6	1.3061023380344403	3.8995455127146075
7	0.8027754403525736	2.79626066616749
8	0.5187046705709855	2.064884107619708
9	0.3627187697355798	1.624419055510235
>10	1.3154896777533704	10.42844620869405
>50	0.03877984792850718	1.295336942380881
>100	0.008811512933833497	0.7041341079307395
>500	9.890487300330196E-5	0.027511548386259826
>1k	5.934292380197991E-4	0.8036915655592367
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4982	0.24456106884281184	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4864	0.2387685746389877	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2143	0.10519758541351781	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.4544466965356466E-5	0.0
12-13	0.0	0.0	0.0	4.908893393071293E-5	0.0
14-15	0.0	0.0	0.0	9.817786786142586E-5	0.0
16-17	0.0	0.0	0.0	9.817786786142586E-5	0.0
18-19	0.0	0.0	0.0	9.817786786142586E-5	0.0
20-21	0.0	0.0	0.0	9.817786786142586E-5	0.0
22-23	0.0	0.0	0.0	2.454446696535647E-4	0.0
24-25	0.0	0.0	0.0	5.399782732378423E-4	0.0
26-27	0.0	0.0	0.0	0.001055412079510328	0.0
28-29	0.0	0.0	0.0	0.0023808132956395773	0.0
30-31	0.0	0.0	0.0	0.005497960600239849	0.0
32-33	0.0	0.0	0.0	0.013254012161292492	0.0
34-35	0.0	0.0	0.0	0.025722601379693576	0.0
36-37	0.0	0.0	0.0	0.04405731820281486	0.0
38-39	0.0	0.0	0.0	0.06325109136972362	0.0
40-41	0.0	0.0	0.0	0.0798922399722353	0.0
42-43	0.0	0.0	0.0	0.09952781354452048	0.0
44-45	0.0	0.0	0.0	0.1263058270037244	0.0
46-47	0.0	0.0	0.0	0.15455650848084967	0.0
48-49	0.0	0.0	0.0	0.18278264549100962	0.0
50-51	0.0	0.0	0.0	0.21032153742613957	0.0
52-53	0.0	0.0	0.0	0.24245024468379117	0.0
54-55	0.0	0.0	0.0	0.279757834471133	0.0
56-57	0.0	0.0	0.0	0.32464966455076993	0.0
58-59	0.0	0.0	0.0	0.3683388157491045	0.0
60-61	0.0	0.0	0.0	0.4100644095902105	0.0
62-63	0.0	0.0	0.0	0.4563307298199074	0.0
64-65	0.0	0.0	0.0	0.5147956501313866	0.0
66-67	0.0	0.0	0.0	0.5890917516355205	0.0
68-69	0.0	0.0	0.0	0.6700639481542315	0.0
70-71	0.0	0.0	0.0	0.7463236070155941	0.0
72-73	0.0	0.0	0.0	0.8271730811994783	0.0
74-75	0.0	0.0	0.0	0.9220129015536156	0.0
76-77	0.0	0.0	0.0	1.024363328799152	0.0
78-79	0.0	0.0	0.0	1.1339298293325033	0.0
80-81	0.0	0.0	0.0	1.2502951472152586	0.0
82-83	0.0	0.0	0.0	1.3733856490465213	0.0
84-85	0.0	0.0	0.0	1.51721622546351	0.0
86-87	0.0	0.0	0.0	1.690254717569273	0.0
88-89	0.0	0.0	0.0	1.8976800078935006	4.908893393071293E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3785	0.0	58.986828	1
GTATCAA	6375	0.0	37.77903	1
GTGGTAT	1635	0.0	34.864746	1
TCAACGC	7300	0.0	32.079365	4
ATCAACG	7430	0.0	31.454151	3
CAACGCA	7520	0.0	31.077705	5
TATCAAC	7680	0.0	30.741014	2
TGGTATC	1785	0.0	30.604315	2
AACGCAG	7900	0.0	29.579922	6
ACGCAGA	8670	0.0	26.678959	7
CGCAGAG	8765	0.0	26.120779	8
GAGTACT	5700	0.0	22.794333	12-13
GCAGAGT	9820	0.0	22.492228	9
CAGAGTA	9365	0.0	21.93849	10-11
GTACTTT	6285	0.0	20.219646	14-15
GTACATG	5585	0.0	19.647705	1
GCGTCAG	475	0.0	19.001287	1
AGAGTAC	8625	0.0	18.31306	10-11
ACATGGG	5775	0.0	18.26008	3
TACATGG	5845	0.0	18.123556	2
>>END_MODULE