FastQCFastQC Report
Fri 10 Feb 2017
SRR2031761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037119
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67910.3333629503234715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67130.32953401347687594No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT31980.15698641071041997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28490.13985437276860113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38400.059.5180131
GTATCAA68150.039.6019941
GTGGTAT17450.032.9476551
TCAACGC83350.032.3135074
TGGTATC17300.032.125242
ATCAACG83900.032.1016773
CAACGCA84250.031.9651785
TATCAAC85550.031.7046322
AACGCAG88100.030.6222026
ACGCAGA98400.027.1272167
CGCAGAG98850.026.7634778
GAGTACT67050.023.3764812-13
GCAGAGT108800.023.2245189
CAGAGTA106700.021.30009310-11
GTACTTT74050.020.74976514-15
AGAGTAC96800.020.01929710-11
GTACATG56750.018.8387131
AGTACTT71650.018.8263512-13
TACATGG57750.018.0135272
ACATGGG57850.017.4076083
Produced by FastQC (version 0.11.5)