##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031761_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2037119 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75269191441443 34.0 31.0 34.0 31.0 34.0 2 32.91471681330349 34.0 31.0 34.0 31.0 34.0 3 32.9655665672943 34.0 31.0 34.0 31.0 34.0 4 36.36251294107021 37.0 37.0 37.0 35.0 37.0 5 36.33299134709362 37.0 37.0 37.0 35.0 37.0 6 36.360387881120346 37.0 37.0 37.0 35.0 37.0 7 36.313148127330805 37.0 37.0 37.0 35.0 37.0 8 36.30484866127114 37.0 37.0 37.0 35.0 37.0 9 38.15457859850112 39.0 39.0 39.0 37.0 39.0 10-11 38.12176068261108 39.0 39.0 39.0 37.0 39.0 12-13 38.06782446189938 39.0 39.0 39.0 35.0 39.0 14-15 39.552772076643535 41.0 39.0 41.0 37.0 41.0 16-17 39.52442640807925 41.0 39.0 41.0 37.0 41.0 18-19 39.53852450445949 41.0 39.5 41.0 37.0 41.0 20-21 39.53854414003305 41.0 39.5 41.0 37.0 41.0 22-23 39.45119185477137 41.0 39.0 41.0 36.0 41.0 24-25 39.38365161779945 41.0 39.0 41.0 36.0 41.0 26-27 39.252262386242535 41.0 39.0 41.0 36.0 41.0 28-29 39.15185784433801 40.5 39.0 41.0 36.0 41.0 30-31 38.9828007102187 40.0 38.5 41.0 35.0 41.0 32-33 38.7700531976777 40.0 38.0 41.0 35.0 41.0 34-35 38.62432435218561 40.0 38.0 41.0 35.0 41.0 36-37 38.52689214523059 40.0 38.0 41.0 35.0 41.0 38-39 38.59793340497045 40.0 38.0 41.0 35.0 41.0 40-41 38.52244149703577 40.0 38.0 41.0 35.0 41.0 42-43 38.41553340771943 40.0 38.0 41.0 34.5 41.0 44-45 38.22407257504348 40.0 38.0 41.0 34.0 41.0 46-47 37.99126388787302 40.0 38.0 41.0 33.5 41.0 48-49 37.730337550236385 40.0 38.0 41.0 33.0 41.0 50-51 37.567021857829616 40.0 38.0 41.0 33.0 41.0 52-53 37.615755387878664 40.0 37.5 41.0 33.0 41.0 54-55 37.53213435248505 40.0 37.0 41.0 33.0 41.0 56-57 37.35331514751961 40.0 37.0 41.0 33.0 41.0 58-59 37.17348446507052 40.0 36.5 41.0 33.0 41.0 60-61 36.91328169832003 39.5 36.0 41.0 32.0 41.0 62-63 36.64021615821167 39.0 35.5 41.0 32.0 41.0 64-65 36.32468206324717 39.0 35.0 41.0 31.0 41.0 66-67 35.98304517310967 38.5 35.0 40.5 31.0 41.0 68-69 35.609108500779776 37.5 35.0 40.0 31.0 41.0 70-71 35.26705852726326 37.0 35.0 39.5 30.0 41.0 72-73 34.88763592112194 36.5 35.0 39.0 30.0 41.0 74-75 34.510430907570935 36.0 35.0 39.0 30.0 40.5 76-77 33.58740701942302 35.0 33.5 37.0 28.5 39.0 78-79 33.79314487764338 35.0 34.0 37.0 29.0 39.0 80-81 33.57272550106302 35.0 34.0 37.0 29.0 39.0 82-83 33.29742960524152 35.0 34.0 36.0 29.0 38.5 84-85 32.99828066008908 35.0 34.0 36.0 29.0 37.0 86-87 32.74569600499529 35.0 34.0 36.0 29.0 37.0 88-89 32.513997954955016 35.0 34.0 35.0 29.0 36.5 90-91 32.30820462623931 35.0 34.0 35.0 27.5 36.0 92-93 32.13358817035235 35.0 34.0 35.0 27.0 36.0 94-95 31.9524242324577 35.0 34.0 35.0 27.0 36.0 96-97 31.78812651592764 35.0 34.0 35.0 26.5 35.5 98-99 31.583089156794472 35.0 33.0 35.0 25.5 35.0 100-101 30.86671814459538 34.5 32.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13934533182886355 1101 2 0.057317955489629924 1101 3 0.12875100403054773 1101 4 0.07145739106033488 1101 5 0.07555673692633036 1101 6 0.10612944931524027 1101 7 0.10513317200643257 1101 8 0.08518112247628551 1101 9 0.04653206916231767 1101 10-11 0.13577584565619105 1101 12-13 0.14036915347836754 1101 14-15 0.17409786324257226 1101 16-17 0.11238683349316858 1101 18-19 0.07487047659491708 1101 20-21 0.05775314832252576 1101 22-23 -0.06594952292788747 1101 24-25 0.022485832694371766 1101 26-27 0.0703098703836389 1101 28-29 0.11089451029721431 1101 30-31 0.14155207140241544 1101 32-33 0.15382477778717174 1101 34-35 -0.10267372391897567 1101 36-37 0.07660919774406949 1101 38-39 0.061598286274325176 1101 40-41 -0.16464046589546655 1101 42-43 -0.06423708678304507 1101 44-45 -0.0423541008447188 1101 46-47 -0.03193725982708884 1101 48-49 -0.09618472582491933 1101 50-51 0.06157127963416542 1101 52-53 -0.01803097340904003 1101 54-55 0.061753552559487446 1101 56-57 -0.25241821514521234 1101 58-59 -0.19652317276813847 1101 60-61 -0.2453845015293652 1101 62-63 -0.12427769795716159 1101 64-65 -0.08430887554803235 1101 66-67 -0.42919738322841283 1101 68-69 -0.6432015096391268 1101 70-71 -0.48809326798056674 1101 72-73 -0.620580318273305 1101 74-75 -0.6184012593386896 1101 76-77 -0.7153452313667685 1101 78-79 -0.6810075553141672 1101 80-81 -0.6081874054805638 1101 82-83 -0.5635043957885486 1101 84-85 -0.5777632981208711 1101 86-87 -0.6056715065851321 1101 88-89 -0.5871186799671619 1101 90-91 -0.6493170789085028 1101 92-93 -0.8116649783822574 1101 94-95 -0.71788695973299 1101 96-97 -0.8658991319619673 1101 98-99 -0.9375664673197193 1101 100-101 -1.2136533147046578 1102 1 0.1870172811780364 1102 2 0.1198618451993525 1102 3 0.12548560962708422 1102 4 0.06767936484729375 1102 5 0.09831197138866798 1102 6 0.07561451417369369 1102 7 0.10443234112155864 1102 8 0.011473095083495366 1102 9 0.10567292633888314 1102 10-11 0.17961569666994848 1102 12-13 0.16389965577327104 1102 14-15 0.17723615304667106 1102 16-17 0.18237792914886342 1102 18-19 0.1878503140133816 1102 20-21 0.22379599591498334 1102 22-23 0.2100725104086223 1102 24-25 0.17292501068575916 1102 26-27 0.13198439272314744 1102 28-29 0.2217156762326269 1102 30-31 0.0573772260346459 1102 32-33 0.28641875092245783 1102 34-35 0.20384849761661172 1102 36-37 0.221845513985933 1102 38-39 0.046547275898092266 1102 40-41 0.06851009898264948 1102 42-43 0.1905008951610725 1102 44-45 0.0906921118595605 1102 46-47 0.11415789025464562 1102 48-49 0.07985444205427683 1102 50-51 0.08517200546462789 1102 52-53 0.07599693623293291 1102 54-55 0.2245429437495261 1102 56-57 0.2762280717151171 1102 58-59 0.3447837153180231 1102 60-61 0.35634131208051656 1102 62-63 0.20115993511643637 1102 64-65 0.22710862865133663 1102 66-67 0.2810596068223177 1102 68-69 0.27114122615745373 1102 70-71 0.18866288219667382 1102 72-73 0.19226061699183816 1102 74-75 0.14354657300122398 1102 76-77 0.21317774296512937 1102 78-79 0.22335782055028375 1102 80-81 0.06456600316985117 1102 82-83 0.06129336042904043 1102 84-85 0.13295084714633987 1102 86-87 0.0774553227379684 1102 88-89 0.04754478076102231 1102 90-91 -0.08198745525047713 1102 92-93 -0.0012615020363568874 1102 94-95 0.08753205005897868 1102 96-97 -0.07999313599560409 1102 98-99 -0.00815937867514549 1102 100-101 0.013922203685481804 1103 1 0.21700986076458406 1103 2 0.08248673807366913 1103 3 0.10686925545388704 1103 4 0.14071687451473736 1103 5 0.1358085747205422 1103 6 0.10707288669935622 1103 7 0.10092724089372496 1103 8 0.13203260470912426 1103 9 0.1426195307158835 1103 10-11 0.15574822420908419 1103 12-13 0.09333975422439522 1103 14-15 0.18402099763603985 1103 16-17 0.16159554583747848 1103 18-19 0.17631862571255397 1103 20-21 0.1899945755851391 1103 22-23 0.107246221242427 1103 24-25 -0.04781661260807368 1103 26-27 0.13397144357910662 1103 28-29 0.12339846400880106 1103 30-31 0.21433921993109095 1103 32-33 0.058443530922872355 1103 34-35 -0.01410785781074253 1103 36-37 0.03816480601577865 1103 38-39 -0.08726577148804182 1103 40-41 -0.07175073900150863 1103 42-43 -0.08057477737921204 1103 44-45 -0.17213945520634866 1103 46-47 -0.1389690040410443 1103 48-49 -0.11366353938424112 1103 50-51 -0.2921475269448095 1103 52-53 -0.23355188172656227 1103 54-55 -0.14451158061242353 1103 56-57 -0.1305941362236709 1103 58-59 -0.14560959272319707 1103 60-61 -0.21830634892155132 1103 62-63 -0.24964716548764443 1103 64-65 -0.14814154902053644 1103 66-67 -0.23892978512182594 1103 68-69 -0.29068858509231177 1103 70-71 -0.24018737176958638 1103 72-73 -0.2445489448902336 1103 74-75 -0.15786134593860623 1103 76-77 -0.17194302165099629 1103 78-79 -0.03530237677000514 1103 80-81 -0.16445826471752412 1103 82-83 -0.1381668306204773 1103 84-85 -0.20509666962797013 1103 86-87 -0.1734101744297405 1103 88-89 -0.1507775264649922 1103 90-91 -0.219485234352085 1103 92-93 -0.22203774521688757 1103 94-95 -0.10149514418863603 1103 96-97 -0.2715403967134016 1103 98-99 -0.12233772465453896 1103 100-101 -0.17364311685547307 1104 1 0.11560000935472914 1104 2 0.0521910202779452 1104 3 0.05110407468870193 1104 4 0.025727697025558882 1104 5 0.03185397076593688 1104 6 1.0552973199651206E-4 1104 7 0.06364076610724823 1104 8 0.112851898028417 1104 9 0.06688389248024151 1104 10-11 0.0722211094319718 1104 12-13 0.017015459150094614 1104 14-15 0.17925689912194542 1104 16-17 0.039637548879476014 1104 18-19 0.10271092710485874 1104 20-21 0.170100899441465 1104 22-23 0.10989334888955682 1104 24-25 0.14883864654719048 1104 26-27 0.17035847996613995 1104 28-29 0.11671848857882594 1104 30-31 0.11701340385527459 1104 32-33 0.18910341158275656 1104 34-35 0.05438108567820876 1104 36-37 0.025803012403983416 1104 38-39 0.13393026220798987 1104 40-41 0.06746111896035245 1104 42-43 0.15446017640574183 1104 44-45 0.20189529382840732 1104 46-47 0.14651493769289914 1104 48-49 0.2327920883213892 1104 50-51 0.30723310368581735 1104 52-53 0.3043927187180415 1104 54-55 0.16501222891138667 1104 56-57 0.24852646164692516 1104 58-59 0.21128829542469418 1104 60-61 0.3363071119419132 1104 62-63 0.31343783509735346 1104 64-65 0.360776053647065 1104 66-67 0.2547302710382269 1104 68-69 0.32914147223775103 1104 70-71 0.36147841739619935 1104 72-73 0.31024732865603966 1104 74-75 0.3004390811118256 1104 76-77 0.3714274592194897 1104 78-79 0.5364698825022529 1104 80-81 0.5056599054006412 1104 82-83 0.5452893528357023 1104 84-85 0.579231895950592 1104 86-87 0.5449815127119493 1104 88-89 0.543347605160136 1104 90-91 0.5519586933418381 1104 92-93 0.44175144982230563 1104 94-95 0.6199129522194688 1104 96-97 0.5502172375442349 1104 98-99 0.5259855174187074 1104 100-101 0.5325037767914189 1105 1 0.04972509479560472 1105 2 0.0307290862220313 1105 3 0.040045584306902526 1105 4 0.041473229688385516 1105 5 0.06924859368687208 1105 6 0.07666869088162542 1105 7 0.10409091745965071 1105 8 0.04774188273043478 1105 9 -5.696439582578705E-4 1105 10-11 0.0729310824163818 1105 12-13 0.15119221597723254 1105 14-15 0.17292817145862927 1105 16-17 0.20962827244163407 1105 18-19 0.17248967592495745 1105 20-21 0.06560874372750192 1105 22-23 0.1384529784830164 1105 24-25 0.26778658457406834 1105 26-27 0.23744380691989164 1105 28-29 0.25938847523377007 1105 30-31 0.2584689095025112 1105 32-33 0.1737505000832229 1105 34-35 0.17351007698764676 1105 36-37 0.27883616346345264 1105 38-39 0.2677530496961893 1105 40-41 0.17734418702011112 1105 42-43 0.08718565894324826 1105 44-45 0.0981090424857669 1105 46-47 0.2436571438576678 1105 48-49 0.3111820567489474 1105 50-51 0.18943861747028024 1105 52-53 0.27558897233910784 1105 54-55 0.23780582003204387 1105 56-57 0.16277230352434202 1105 58-59 0.18682169060095077 1105 60-61 0.2631697230150465 1105 62-63 0.0430337404451393 1105 64-65 0.12348436663733509 1105 66-67 0.17420428073779703 1105 68-69 0.05091953753085221 1105 70-71 -0.060015658703143515 1105 72-73 -0.1743611080200651 1105 74-75 0.11167984536010778 1105 76-77 -0.04193956223024031 1105 78-79 -0.036118243657298876 1105 80-81 -0.10472156776278752 1105 82-83 0.049805513366898424 1105 84-85 0.061311689590532126 1105 86-87 0.050673502426498374 1105 88-89 0.03625194392913755 1105 90-91 0.011181018635603834 1105 92-93 0.021030410210286732 1105 94-95 -0.010762763042716728 1105 96-97 0.12300472574525756 1105 98-99 0.08787319114848557 1105 100-101 0.0634862654806767 1106 1 0.1840031970761018 1106 2 0.17214871409927923 1106 3 0.17211942297677751 1106 4 0.13847858250371559 1106 5 0.07743149929800808 1106 6 0.10124681269146407 1106 7 0.17329247803168357 1106 8 0.17318739586391985 1106 9 0.20964264887535933 1106 10-11 0.14919621765707802 1106 12-13 0.1410069979143671 1106 14-15 0.22637171612311846 1106 16-17 0.2659879030707444 1106 18-19 0.2271083100249669 1106 20-21 0.24401372903611218 1106 22-23 0.25152066097141557 1106 24-25 0.34052007118315686 1106 26-27 0.31813494592442737 1106 28-29 0.23870540317937383 1106 30-31 0.3240062216435433 1106 32-33 0.39259121166146116 1106 34-35 0.31677777989308 1106 36-37 0.444191442156054 1106 38-39 0.3769448234043722 1106 40-41 0.4856281791728705 1106 42-43 0.38788205755489713 1106 44-45 0.3803929325533133 1106 46-47 0.33557682277751155 1106 48-49 0.3512124936736072 1106 50-51 0.39598999073816543 1106 52-53 0.38977986944610166 1106 54-55 0.2720689978999715 1106 56-57 0.3922936792777776 1106 58-59 0.4054662765344901 1106 60-61 0.4317173834885466 1106 62-63 0.35659259700211976 1106 64-65 0.4772656246820901 1106 66-67 0.43253920452897887 1106 68-69 0.5098405080504236 1106 70-71 0.3123589762313017 1106 72-73 0.3546688162593554 1106 74-75 0.3674648583648761 1106 76-77 0.38940758515415297 1106 78-79 0.3159489261858397 1106 80-81 0.302344281630468 1106 82-83 0.394204642091907 1106 84-85 0.398655843215451 1106 86-87 0.37418261464032554 1106 88-89 0.35087908882798047 1106 90-91 0.42689720180307944 1106 92-93 0.4135022369139989 1106 94-95 0.4867591741793227 1106 96-97 0.4018504528138074 1106 98-99 0.5933433489810831 1106 100-101 0.6621402388142457 1107 1 0.17919402403570217 1107 2 0.14524108615410114 1107 3 0.18506793691343404 1107 4 0.10435559087084556 1107 5 0.12024565154892031 1107 6 0.08588569159911685 1107 7 0.13097560518121298 1107 8 0.1564948492800653 1107 9 0.11758500296396335 1107 10-11 0.09959500365368967 1107 12-13 0.14809723968839705 1107 14-15 0.23420466874362234 1107 16-17 0.20148114007089646 1107 18-19 0.1872088567114858 1107 20-21 0.2362240878472548 1107 22-23 0.2506448075757959 1107 24-25 0.24404145098912267 1107 26-27 0.2512332211669701 1107 28-29 0.242588994884116 1107 30-31 0.3530970665693758 1107 32-33 0.17723394047415297 1107 34-35 0.10548215851840581 1107 36-37 0.2637688509133014 1107 38-39 0.179678897630545 1107 40-41 0.18148568135448073 1107 42-43 0.14695500042230236 1107 44-45 0.22539896407120352 1107 46-47 0.14223298775877424 1107 48-49 0.18237330420695486 1107 50-51 0.11021635232123828 1107 52-53 0.2487626603705948 1107 54-55 0.10630010769926201 1107 56-57 0.15194731615473955 1107 58-59 0.1214026376042483 1107 60-61 0.13613771612033787 1107 62-63 0.12764355691177798 1107 64-65 0.15233684792525537 1107 66-67 0.2452252832723687 1107 68-69 0.3548449354412071 1107 70-71 0.3754653362744236 1107 72-73 0.17240147885367207 1107 74-75 0.2994979932142101 1107 76-77 0.3115977961289502 1107 78-79 0.2704478610454544 1107 80-81 0.31165463530841464 1107 82-83 0.34653158886055024 1107 84-85 0.37342773634208726 1107 86-87 0.41099412328977536 1107 88-89 0.3875975581057389 1107 90-91 0.4166420172643015 1107 92-93 0.35315978786108104 1107 94-95 0.2914262215588188 1107 96-97 0.3677402941977199 1107 98-99 0.44789874911454675 1107 100-101 0.5310686177475645 1108 1 0.08847695965023661 1108 2 0.005044723008253982 1108 3 0.025184535195620583 1108 4 -0.0010443956399228682 1108 5 0.10821570544455028 1108 6 0.05925754915055137 1108 7 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54-55 -0.30743167356916246 2316 56-57 -0.28754167412625264 2316 58-59 -0.41333299408307766 2316 60-61 -0.4205636540457718 2316 62-63 -0.32366851880378533 2316 64-65 -0.3003641483628172 2316 66-67 -0.23044478510572475 2316 68-69 -0.3365521551335675 2316 70-71 -0.12918885985045137 2316 72-73 -0.26231824484803923 2316 74-75 -0.1823547904914875 2316 76-77 -0.5235808366200558 2316 78-79 -0.26589431568783084 2316 80-81 -0.0818383727483365 2316 82-83 -0.01731018563083353 2316 84-85 0.04251690524471741 2316 86-87 -0.03433913386825793 2316 88-89 -0.11634653218862923 2316 90-91 -0.210266158733738 2316 92-93 -0.10391136221966235 2316 94-95 -0.05826148328425518 2316 96-97 -0.10919443884992219 2316 98-99 -0.13334664428206722 2316 100-101 0.13033141989078345 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 3.0 5 17.0 6 51.0 7 109.0 8 283.0 9 543.0 10 1003.0 11 1622.0 12 2578.0 13 3663.0 14 4507.0 15 5895.0 16 7886.0 17 11726.0 18 11979.0 19 7428.0 20 5314.0 21 5248.0 22 5655.0 23 5978.0 24 6976.0 25 8190.0 26 9483.0 27 10667.0 28 13644.0 29 17757.0 30 22619.0 31 29289.0 32 37923.0 33 50740.0 34 72844.0 35 115686.0 36 207113.0 37 439407.0 38 678278.0 39 234795.0 40 217.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.771845506289615 20.199836112980904 14.607007471146705 23.421310909582775 2 16.2037171122551 21.96062183897946 38.997770871510205 22.837890177255233 3 18.464772352962257 25.991971838107407 31.49706025146791 24.046195557462426 4 13.703245280647378 19.340741457197243 37.16172592625688 29.7942873358985 5 13.835323316899995 36.860193243497314 34.92402751140213 14.380455928200561 6 29.144590964003577 37.0852169166357 18.857612147351237 14.91257997200949 7 25.130785192224902 33.232079225612246 23.574126008348063 18.063009573814785 8 23.97047006090464 34.34713435984839 22.072593697275416 19.60980188197155 9 25.11983835995835 16.74884972355567 21.98187734737146 36.14943456911452 10-11 23.46955355556232 26.542694139465656 29.409612010693536 20.578140294278484 12-13 24.567256290504528 25.17289131017447 28.702264669989663 21.55758772933134 14-15 22.161720096302695 26.932846567345003 26.87570703422506 24.029726302127244 16-17 21.23800622257741 29.258656186398497 27.879905630460588 21.623431960563508 18-19 21.103182958051693 27.7174896158267 30.136573079225542 21.042754346896064 20-21 22.055412570399668 26.905620142956792 29.89808155537305 21.140885731270487 22-23 21.906525833787814 27.01638932237145 29.45021866665619 21.626866177184542 24-25 21.592184845362496 27.34972772822795 29.704253899747634 21.35383352666192 26-27 21.347221959731687 27.49904828432233 29.987597629092775 21.166132126853206 28-29 21.31073461304711 27.31883076143657 30.12933611779572 21.241098507720597 30-31 21.706332599862304 27.09465703377444 29.85038909144907 21.348621274914187 32-33 21.592458743104213 27.19052541978505 29.65626862316002 21.56074721395072 34-35 21.579186122379646 27.54099262061642 29.61236020975104 21.267461047252898 36-37 21.629608829670094 27.33997499313956 29.661528658480613 21.368887518709737 38-39 21.389005747823273 27.346487858588524 29.84582638520381 21.41868000838439 40-41 21.565210476167568 27.260606768676745 29.74968570810051 21.42449704705518 42-43 21.71944790657787 27.207737986833365 29.54270221818166 21.53011188840711 44-45 21.690976324898052 27.50639506086782 29.202074105636438 21.600554508597682 46-47 21.70965466425869 27.41867313593364 29.09064222561372 21.781029974193945 48-49 21.630081502357005 27.519673617496082 29.123973611752675 21.726271268394235 50-51 21.6589212510413 27.754097821482198 28.825193815383393 21.76178711209311 52-53 21.79236951793194 27.85745457187332 28.77497583597227 21.575200074222465 54-55 21.788270591948727 27.87473387661693 28.54484691370509 21.792148617729254 56-57 21.810139712014863 28.048705058467377 28.395861999225374 21.74529323029239 58-59 21.689401051733764 27.955917996674213 28.73596819026932 21.618712761322705 60-61 21.85792135126551 28.024042221156424 28.626791348217033 21.491245079361025 62-63 21.581422590075093 28.172557121014 28.593092874530758 21.65292741438015 64-65 21.662664625515227 28.10409774160191 28.499368337755094 21.733869295127768 66-67 21.61862707317253 28.162157253066344 28.400592036955675 21.818623636805455 68-69 21.57924733647872 28.153578271419725 28.556978467486704 21.71019592461485 70-71 21.612272828574486 27.98576223861357 28.529569715647302 21.87239521716464 72-73 21.677036181740572 27.98689039978537 28.44072390481892 21.895349513655137 74-75 21.68758687776619 28.06070539039183 28.28557198860768 21.966135743234304 76-77 21.61893494609932 28.09141025389283 28.269091051897817 22.02056374811004 78-79 21.567199054347526 28.07992393152659 28.29198885481014 22.060888159315738 80-81 21.58696051315132 28.149610551295982 28.268683768437818 21.99474516711488 82-83 21.717020346436065 28.109889262897337 28.198060670863644 21.97502971980295 84-85 21.78211163086149 28.083382709346523 28.065931336333367 22.068574323458623 86-87 21.654327969301264 28.110605978046106 28.176091644588574 22.058974408064064 88-89 21.725094152898812 28.000006872474366 28.116667124831622 22.1582318497952 90-91 21.686607409778222 28.01996348765094 28.180017956732033 22.113411145838803 92-93 21.703886714521833 27.901389167741304 28.23384397278706 22.160880144949804 94-95 21.672175263202593 27.871224017840884 28.161707784375878 22.294892934580652 96-97 21.67831137994393 27.811482785247204 28.16057873889547 22.349627095913398 98-99 21.503235697080044 27.892602248567705 28.126928274685962 22.477233779666285 100-101 21.541969537615312 27.88168489312639 28.11004846344256 22.466297105815734 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2067.0 1 1245.5 2 444.0 3 448.5 4 347.0 5 319.5 6 547.5 7 1097.0 8 1784.0 9 2144.5 10 1839.0 11 1354.5 12 1270.5 13 1432.0 14 1635.5 15 1763.5 16 1869.0 17 1978.0 18 2116.0 19 2283.5 20 2569.5 21 2952.5 22 3381.5 23 4007.0 24 4790.0 25 6078.5 26 8301.0 27 11417.5 28 15369.0 29 21323.0 30 29386.0 31 37815.0 32 46821.0 33 55746.5 34 62679.5 35 66388.5 36 68876.0 37 70977.0 38 70449.5 39 68223.0 40 67110.5 41 67650.0 42 67946.5 43 69783.0 44 71899.5 45 72456.5 46 73080.0 47 73524.5 48 74003.0 49 72823.0 50 69569.5 51 65979.5 52 62830.5 53 60086.5 54 56520.5 55 52179.5 56 47118.5 57 41308.0 58 36986.5 59 33131.0 60 27815.0 61 22522.5 62 17676.0 63 13116.0 64 9410.0 65 6770.0 66 4933.5 67 3783.0 68 2942.5 69 2124.0 70 1548.5 71 1179.0 72 876.5 73 635.5 74 464.0 75 335.0 76 215.0 77 108.5 78 57.5 79 37.0 80 25.5 81 17.0 82 13.0 83 8.0 84 2.5 85 2.5 86 3.0 87 2.5 88 0.5 89 0.0 90 1.0 91 1.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017181126875749526 2 0.0 3 0.0019635573572285175 4 0.016984771140026677 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.908893393071293E-5 12-13 4.908893393071293E-5 14-15 2.4544466965356466E-5 16-17 0.0010308676125449715 18-19 2.2090020268820823E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.1725593841105996E-4 28-29 0.004957982327002006 30-31 7.36334008960694E-5 32-33 2.454446696535647E-4 34-35 0.011192276936202549 36-37 0.003828936846595609 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.190780705496341E-4 60-61 0.0017672016215056659 62-63 0.009498708715592953 64-65 0.0016444792866788832 66-67 0.004123470450179886 68-69 0.002945336035842776 70-71 4.908893393071293E-5 72-73 0.006798817349403742 74-75 0.006553372679750176 76-77 0.0019144684232978045 78-79 3.4362253751499055E-4 80-81 0.006725183948507672 82-83 0.008713285772701546 84-85 0.001472668017921388 86-87 0.0015708458857828138 88-89 3.4362253751499055E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.099674098567633E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2037119.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.68491358562454 #Duplication Level Percentage of deduplicated Percentage of total 1 58.91331551065646 28.092763591682875 2 20.7124273488836 19.753406165600822 3 8.989920565303295 12.860507558943498 4 4.3008605526059975 8.203446551793537 5 2.3666889816525423 5.642767978707562 6 1.3891501246854712 3.9744902191851743 7 0.8474816418599903 2.8288462200247793 8 0.5473114516301449 2.087879942032495 9 0.41032412413460323 1.760964336131007 >10 1.4679639934796078 11.122000560738877 >50 0.04279586436815292 1.3780381864586415 >100 0.010522753593048823 0.8923920782607521 >500 4.1236238238073015E-4 0.14079040631967601 >1k 6.185435735710694E-4 0.5978676402443603 >5k 2.0618119119035643E-4 0.6638385638759943 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6791 0.3333629503234715 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6713 0.32953401347687594 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 3198 0.15698641071041997 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2849 0.13985437276860113 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.4544466965356466E-5 0.0 12-13 0.0 0.0 0.0 4.908893393071293E-5 0.0 14-15 0.0 0.0 0.0 9.817786786142586E-5 0.0 16-17 0.0 0.0 0.0 9.817786786142586E-5 0.0 18-19 0.0 0.0 0.0 9.817786786142586E-5 0.0 20-21 0.0 0.0 0.0 9.817786786142586E-5 0.0 22-23 0.0 0.0 0.0 2.454446696535647E-4 0.0 24-25 0.0 0.0 0.0 6.136116741339117E-4 0.0 26-27 0.0 0.0 0.0 0.001153589947371754 0.0 28-29 0.0 0.0 0.0 0.0025035356304663595 0.0 30-31 0.0 0.0 0.0 0.005596138468101274 0.0 32-33 0.0 0.0 0.0 0.013524001297911413 0.0 34-35 0.0 0.0 0.0 0.025648967978797507 0.0 36-37 0.0 0.0 0.0 0.04349279546261166 0.0 38-39 0.0 0.0 0.0 0.06261293522862435 0.0 40-41 0.0 2.4544466965356466E-5 0.0 0.07920499489720531 0.0 42-43 0.0 4.908893393071293E-5 0.0 0.09884056846949049 0.0 44-45 0.0 4.908893393071293E-5 0.0 0.12591311553227869 0.0 46-47 0.0 4.908893393071293E-5 0.0 0.1542619748772654 0.0 48-49 0.0 4.908893393071293E-5 0.0 0.18265992315618285 0.0 50-51 0.0 4.908893393071293E-5 0.0 0.21037062636007028 0.0 52-53 0.0 4.908893393071293E-5 0.0 0.24249933361772189 0.0 54-55 0.0 4.908893393071293E-5 0.0 0.28012600147561334 0.0 56-57 0.0 4.908893393071293E-5 0.0 0.32558235429545357 0.0 58-59 0.0 4.908893393071293E-5 0.0 0.36959058356433766 0.0 60-61 0.0 4.908893393071293E-5 0.0 0.41138981080633974 0.0 62-63 0.0 4.908893393071293E-5 0.0 0.4580488425074824 0.0 64-65 0.0 4.908893393071293E-5 0.0 0.5164892183519961 0.0 66-67 0.0 4.908893393071293E-5 0.0 0.5907362309221995 0.0 68-69 0.0 4.908893393071293E-5 0.0 0.6722484057141482 0.0 70-71 0.0 4.908893393071293E-5 0.0 0.7485080645755109 0.0 72-73 0.0 4.908893393071293E-5 0.0 0.8293329942924297 0.0 74-75 0.0 4.908893393071293E-5 0.0 0.9246146150519434 0.0 76-77 0.0 4.908893393071293E-5 0.0 1.0268177754956878 0.0 78-79 0.0 4.908893393071293E-5 0.0 1.1375869549103417 0.0 80-81 0.0 4.908893393071293E-5 0.0 1.2542468063966807 0.0 82-83 0.0 4.908893393071293E-5 0.0 1.3777545641663544 0.0 84-85 0.0 4.908893393071293E-5 0.0 1.5222478411914082 0.0 86-87 0.0 4.908893393071293E-5 0.0 1.6960472117730974 0.0 88-89 0.0 7.36334008960694E-5 0.0 1.905288792652761 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3840 0.0 59.518013 1 GTATCAA 6815 0.0 39.601994 1 GTGGTAT 1745 0.0 32.947655 1 TCAACGC 8335 0.0 32.313507 4 TGGTATC 1730 0.0 32.12524 2 ATCAACG 8390 0.0 32.101677 3 CAACGCA 8425 0.0 31.965178 5 TATCAAC 8555 0.0 31.704632 2 AACGCAG 8810 0.0 30.622202 6 ACGCAGA 9840 0.0 27.127216 7 CGCAGAG 9885 0.0 26.763477 8 GAGTACT 6705 0.0 23.37648 12-13 GCAGAGT 10880 0.0 23.224518 9 CAGAGTA 10670 0.0 21.300093 10-11 GTACTTT 7405 0.0 20.749765 14-15 AGAGTAC 9680 0.0 20.019297 10-11 GTACATG 5675 0.0 18.838713 1 AGTACTT 7165 0.0 18.82635 12-13 TACATGG 5775 0.0 18.013527 2 ACATGGG 5785 0.0 17.407608 3 >>END_MODULE