FastQCFastQC Report
Fri 10 Feb 2017
SRR2031760_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031760_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49534
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8011.617071102676949No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6921.3970202285298987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6701.3526062906286591No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.7489804982436306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.6863972221100658No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.33108571890014943No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.207937981992167No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.11103484475309888No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.10699721403480437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA510.10295958331650987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTAAA250.004992950757.06
GGTATCA4300.047.51
GTATAAA550.002555898434.5454561
GTATCAA6350.032.165352
GAGCAAT350.00835514727.14285510-11
TATCAAC7900.025.854433
ATCAACG8050.024.7826084
TCAACGC8100.024.629634
AACGCAG8400.023.757
CAACGCA8500.023.4705896
GAGTACT5250.022.16666612-13
GTGGTAT2005.1743336E-721.3751
GTACTTT5450.021.3532114-15
ACGCAGA9450.021.1111138
CGCAGAG9500.021.09
TGGTATC2108.2707265E-720.3571432
ACTTTTT6150.019.30894316-17
GTACATG4950.019.1919211
TACATGG4950.019.1919212
CAGAGTA9550.019.14921610-11
Produced by FastQC (version 0.11.5)