##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031760_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 49534 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.006924536681876 31.0 30.0 34.0 10.0 34.0 2 28.276194129284935 33.0 30.0 34.0 10.0 34.0 3 28.309343077482133 33.0 30.0 34.0 10.0 34.0 4 31.41920700932693 37.0 35.0 37.0 10.0 37.0 5 31.374732506964914 37.0 35.0 37.0 2.0 37.0 6 31.375459280494205 37.0 35.0 37.0 2.0 37.0 7 31.297169620866477 37.0 35.0 37.0 2.0 37.0 8 31.25101950175637 37.0 35.0 37.0 2.0 37.0 9 32.659930552751646 39.0 35.0 39.0 2.0 39.0 10-11 32.52102596196552 39.0 35.0 39.0 2.0 39.0 12-13 32.40760891508863 39.0 34.5 39.0 2.0 39.0 14-15 33.25574352969677 40.0 34.0 41.0 2.0 41.0 16-17 33.141942907901644 40.0 34.0 41.0 2.0 41.0 18-19 33.16340291516938 40.0 34.0 41.0 2.0 41.0 20-21 33.17289134735737 40.0 34.5 41.0 2.0 41.0 22-23 33.01366737998143 40.0 34.0 41.0 2.0 41.0 24-25 32.829591795534384 40.0 34.0 41.0 2.0 41.0 26-27 32.58564824161182 40.0 34.0 41.0 2.0 41.0 28-29 32.33813138450357 39.0 33.5 41.0 2.0 41.0 30-31 31.940444946905156 38.5 33.0 41.0 2.0 41.0 32-33 31.430532563491745 38.0 32.5 41.0 2.0 41.0 34-35 31.113396858723302 38.0 31.5 41.0 2.0 41.0 36-37 30.843844631969958 37.5 31.0 40.5 2.0 41.0 38-39 30.481093794161588 37.0 30.0 40.0 2.0 41.0 40-41 30.1950781281544 37.0 30.0 40.0 2.0 41.0 42-43 29.784249202567935 37.0 26.5 40.0 2.0 41.0 44-45 29.23417248758429 36.5 23.0 40.0 2.0 41.0 46-47 28.535167763556345 36.5 11.5 40.5 2.0 41.0 48-49 27.95385997496669 36.5 2.0 41.0 2.0 41.0 50-51 27.274023902773852 35.5 2.0 40.0 2.0 40.5 52-53 27.144355392255825 35.0 2.0 40.0 2.0 41.0 54-55 27.177857633140874 35.0 2.0 40.0 2.0 41.0 56-57 26.9471978842815 35.0 2.0 40.0 2.0 41.0 58-59 26.670065005854564 35.0 2.0 40.0 2.0 41.0 60-61 26.359349133928212 35.0 2.0 40.0 2.0 41.0 62-63 26.00172608713207 35.0 2.0 39.0 2.0 41.0 64-65 25.683550692453668 35.0 2.0 39.0 2.0 41.0 66-67 25.332731053417852 35.0 2.0 39.0 2.0 41.0 68-69 24.987513627003672 35.0 2.0 37.5 2.0 41.0 70-71 24.583962530786934 35.0 2.0 37.0 2.0 40.0 72-73 24.19902491218153 35.0 2.0 36.5 2.0 39.0 74-75 23.812744781362298 34.0 2.0 36.0 2.0 39.0 76-77 23.48499010780474 34.0 2.0 35.5 2.0 39.0 78-79 23.143315702345866 34.0 2.0 35.0 2.0 37.0 80-81 22.839695966406914 34.0 2.0 35.0 2.0 37.0 82-83 22.557869342269957 34.0 2.0 35.0 2.0 36.5 84-85 22.285642185165745 33.5 2.0 35.0 2.0 36.0 86-87 22.020167965437878 33.0 2.0 35.0 2.0 36.0 88-89 21.730760689627328 33.0 2.0 35.0 2.0 35.5 90-91 21.465993055275163 33.0 2.0 35.0 2.0 35.0 92-93 21.212581257318206 32.5 2.0 35.0 2.0 35.0 94-95 20.962318811321516 32.0 2.0 35.0 2.0 35.0 96-97 20.69680421528647 32.0 2.0 35.0 2.0 35.0 98-99 20.42537449024912 31.5 2.0 35.0 2.0 35.0 100-101 20.015120927040016 30.5 2.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.235664334882042 1101 2 -0.7585910890797862 1101 3 -0.43568076915887843 1101 4 -0.23672674434338603 1101 5 -1.2415725473797004 1101 6 -0.9846918628354153 1101 7 -2.268435884105873 1101 8 -1.688959039468287 1101 9 -1.0981911377722504 1101 10-11 -1.697717240050384 1101 12-13 -1.6812873427390542 1101 14-15 -2.2265291134090255 1101 16-17 -1.4769851517076162 1101 18-19 -1.4837680910755964 1101 20-21 -1.9377221665217945 1101 22-23 -1.5225688885288946 1101 24-25 -1.5780682575445084 1101 26-27 -1.2841219928315226 1101 28-29 -2.4397579889790073 1101 30-31 -2.0723361566947105 1101 32-33 -2.738904024481446 1101 34-35 -2.932943308374064 1101 36-37 -2.668713165430315 1101 38-39 -2.654066941343121 1101 40-41 -2.757811298553989 1101 42-43 -2.579284393749031 1101 44-45 -2.2768472087532707 1101 46-47 -2.3444519431325404 1101 48-49 -2.4102036626459196 1101 50-51 -1.7507724681032215 1101 52-53 -2.747609479710892 1101 54-55 -2.0618071463310486 1101 56-57 -1.9081098747222285 1101 58-59 -2.388546512162801 1101 60-61 -1.7767779395753038 1101 62-63 -1.4270193935315731 1101 64-65 -1.2402715243367908 1101 66-67 -1.1375813658759597 1101 68-69 -1.1600590927981074 1101 70-71 -1.5436854635335102 1101 72-73 -1.123817945082692 1101 74-75 -1.0410891245221237 1101 76-77 -1.423132821733077 1101 78-79 -0.928170354323413 1101 80-81 -0.9168573381723704 1101 82-83 -1.4575817400811104 1101 84-85 -2.3707464882238583 1101 86-87 -1.8672759895608877 1101 88-89 -2.793614640207938 1101 90-91 -3.362604569368326 1101 92-93 -2.4850630034500654 1101 94-95 -2.379887605830149 1101 96-97 -2.055612216989502 1101 98-99 -1.819955096895427 1101 100-101 -1.491527554284243 1102 1 1.001852534213775 1102 2 2.0254844844694695 1102 3 0.9792989879261427 1102 4 0.5304797333894093 1102 5 1.1936501246850746 1102 6 1.1354160993778137 1102 7 1.783520931008841 1102 8 0.6558452790202409 1102 9 1.3984350430644596 1102 10-11 0.8444554994907776 1102 12-13 1.1189825627940095 1102 14-15 1.5015410620295668 1102 16-17 1.939681514959048 1102 18-19 1.997675903526293 1102 20-21 1.721522097985627 1102 22-23 1.8350559427801443 1102 24-25 1.3493946304446922 1102 26-27 1.143340895157678 1102 28-29 1.4347359381465026 1102 30-31 1.5467596597695312 1102 32-33 1.8984104154645784 1102 34-35 2.306935234136059 1102 36-37 2.314080356836893 1102 38-39 2.6718439898309647 1102 40-41 3.194955233159913 1102 42-43 3.8923755252793093 1102 44-45 3.140156839829725 1102 46-47 3.0172214711724514 1102 48-49 3.4663145559775614 1102 50-51 3.5241937937051446 1102 52-53 3.053672571571159 1102 54-55 2.941229290915917 1102 56-57 2.7491100982872183 1102 58-59 2.1090918144228965 1102 60-61 2.3824663249321176 1102 62-63 2.6016580693564144 1102 64-65 1.7546677469182725 1102 66-67 2.0054685666476573 1102 68-69 1.8750286265001357 1102 70-71 2.166166088423303 1102 72-73 2.2780039172654867 1102 74-75 1.3674803761526384 1102 76-77 1.5684326303586893 1102 78-79 1.4436245174714593 1102 80-81 0.5662735660111657 1102 82-83 0.9051038199728687 1102 84-85 1.37048158195158 1102 86-87 0.7521572088196748 1102 88-89 1.4735918375248538 1102 90-91 2.143468305125598 1102 92-93 2.261563177386641 1102 94-95 2.4328654306070945 1102 96-97 2.7682744226866127 1102 98-99 1.7738370758441135 1102 100-101 1.5398489639343786 1103 1 -5.853458821097579 1103 2 -5.20345324446826 1103 3 -4.89432738570023 1103 4 -6.339483636573963 1103 5 -6.17265024161529 1103 6 -6.133814669852956 1103 7 -6.170691523203615 1103 8 -6.1225429993680365 1103 9 -7.110722466093051 1103 10-11 -6.160123255087974 1103 12-13 -5.935046741235297 1103 14-15 -6.319887509398999 1103 16-17 -6.286508961231426 1103 18-19 -5.802690396840006 1103 20-21 -5.655767279303749 1103 22-23 -5.456152848428644 1103 24-25 -5.680825149775089 1103 26-27 -4.958307456490672 1103 28-29 -5.3583043548937965 1103 30-31 -5.534742171732304 1103 32-33 -5.181259914205754 1103 34-35 -4.9229182457174225 1103 36-37 -5.018337225580691 1103 38-39 -5.074493006506028 1103 40-41 -5.468964180759503 1103 42-43 -6.077404694500917 1103 44-45 -6.251784552111669 1103 46-47 -5.143950689999709 1103 48-49 -4.690278850615847 1103 50-51 -4.217564448053096 1103 52-53 -5.0452285273299395 1103 54-55 -4.44338609369947 1103 56-57 -4.217007117829997 1103 58-59 -3.6116041562730743 1103 60-61 -3.2805373380715466 1103 62-63 -3.689550721852374 1103 64-65 -3.997163754913231 1103 66-67 -3.368157806978715 1103 68-69 -3.422590421118908 1103 70-71 -4.299951127693912 1103 72-73 -4.369431980170408 1103 74-75 -4.118783360111095 1103 76-77 -3.6806516187255625 1103 78-79 -4.809122735275793 1103 80-81 -4.181894932157334 1103 82-83 -3.479511564642511 1103 84-85 -3.819994608524606 1103 86-87 -3.4117622050997376 1103 88-89 -4.17018105624804 1103 90-91 -3.554333892676599 1103 92-93 -3.0214038555803917 1103 94-95 -2.6944239466822815 1103 96-97 -3.6026046981925077 1103 98-99 -3.852536550529514 1103 100-101 -3.077367153281738 1104 1 -0.826164976730567 1104 2 -0.5454973791953215 1104 3 -0.8368360965016244 1104 4 -1.2181131371546812 1104 5 -0.65813223929241 1104 6 -0.15413987310499166 1104 7 -2.046998143412676 1104 8 -0.21720037451321872 1104 9 -0.9492822802626222 1104 10-11 -0.4310291784329223 1104 12-13 0.07801946085529465 1104 14-15 -0.2708166615476628 1104 16-17 0.1266733848777548 1104 18-19 -0.012330350382399047 1104 20-21 -0.166220240976223 1104 22-23 0.36382392276763653 1104 24-25 0.4222526667173625 1104 26-27 -0.10087423930135486 1104 28-29 0.20209053476938976 1104 30-31 -0.19589199751627362 1104 32-33 0.1773347431693928 1104 34-35 0.8491491178133579 1104 36-37 -0.9495218945702355 1104 38-39 -1.663052820675599 1104 40-41 -1.1974775373216389 1104 42-43 -1.2943036492047462 1104 44-45 -0.4527137042603293 1104 46-47 -0.5684570779207299 1104 48-49 -0.18940777047647117 1104 50-51 -0.02708825757690647 1104 52-53 -0.5098046016621112 1104 54-55 -0.004361703455568744 1104 56-57 0.1137129750852175 1104 58-59 -0.27333470214996325 1104 60-61 -1.2247882091516828 1104 62-63 -0.7476227311438954 1104 64-65 0.0013844448544801935 1104 66-67 -0.07082911940612746 1104 68-69 -0.9013941377275039 1104 70-71 -1.127767620144553 1104 72-73 -1.355204337893987 1104 74-75 -1.253220061620972 1104 76-77 -0.7398850682029128 1104 78-79 -1.2574386470063388 1104 80-81 -1.0869471969656956 1104 82-83 -1.1421131906587725 1104 84-85 -0.962851751381752 1104 86-87 -0.8923080819870748 1104 88-89 -0.45792786228753 1104 90-91 -0.3208844153246808 1104 92-93 -1.3788370727697057 1104 94-95 -2.0117874774604445 1104 96-97 -2.6069600988893704 1104 98-99 -1.7474262137118615 1104 100-101 -1.9263799291237795 1105 1 0.8911299234911638 1105 2 0.8203562793412651 1105 3 1.2939843026114595 1105 4 1.0252349781446561 1105 5 1.004838935873888 1105 6 1.267700481662196 1105 7 1.5306071780950852 1105 8 0.5538639270388899 1105 9 0.9520480966775082 1105 10-11 1.1970196020548798 1105 12-13 1.0406026094140586 1105 14-15 0.8500259105144252 1105 16-17 0.2502875755651104 1105 18-19 0.5993658802162756 1105 20-21 1.0341677856313183 1105 22-23 0.9702540779782822 1105 24-25 1.5248025558526237 1105 26-27 1.2732942751110627 1105 28-29 0.8814142598248225 1105 30-31 1.0951279581378266 1105 32-33 1.0368136788678406 1105 34-35 0.32004712224794574 1105 36-37 0.31932511208164627 1105 38-39 1.0337321017190781 1105 40-41 0.6955379837426641 1105 42-43 0.6904524483562291 1105 44-45 -0.12732567765279157 1105 46-47 -0.6482796943287106 1105 48-49 -0.7714476817846787 1105 50-51 -0.7316337121223597 1105 52-53 -0.8839731160745288 1105 54-55 -0.7047497300631065 1105 56-57 -1.1060763818992605 1105 58-59 -0.8556517753206947 1105 60-61 -0.5435243510585579 1105 62-63 -0.8313256136272678 1105 64-65 -0.9149126726621475 1105 66-67 -0.31999763381853796 1105 68-69 -0.5757289492574387 1105 70-71 -1.1776567553995427 1105 72-73 -1.7709471316855598 1105 74-75 -1.231878598206336 1105 76-77 -0.5929892810632218 1105 78-79 -0.4622612634143195 1105 80-81 -0.3702066204690233 1105 82-83 -0.9832994434303934 1105 84-85 -1.9514881150181154 1105 86-87 -2.2434721618096916 1105 88-89 -2.1339256449926296 1105 90-91 -2.2053079186506217 1105 92-93 -2.69618740536394 1105 94-95 -1.3075191847775187 1105 96-97 -1.378578724166534 1105 98-99 -1.1847876327805977 1105 100-101 -1.4042069801215646 1106 1 1.2600516993896846 1106 2 1.866867907248242 1106 3 1.725273943203426 1106 4 2.288977050026258 1106 5 2.755367477100105 1106 6 3.001844244875727 1106 7 1.6648566495538581 1106 8 1.9909675211192237 1106 9 2.526043868347699 1106 10-11 2.5167870439153432 1106 12-13 2.482727345143438 1106 14-15 1.6216961007892579 1106 16-17 2.2497590343388936 1106 18-19 0.42045467514227397 1106 20-21 1.2146646143839561 1106 22-23 1.7038692999655964 1106 24-25 1.139496001941417 1106 26-27 1.2706515689799787 1106 28-29 1.2585284247296826 1106 30-31 1.770075104015202 1106 32-33 1.9651963427156183 1106 34-35 1.4804593844043943 1106 36-37 2.346280714618647 1106 38-39 2.5332035604928684 1106 40-41 1.6675252987532367 1106 42-43 2.978243146030646 1106 44-45 2.556078127844035 1106 46-47 1.423601912436613 1106 48-49 2.9600533752977753 1106 50-51 3.240353602888213 1106 52-53 3.6535533109867835 1106 54-55 3.8007999528121594 1106 56-57 3.014166747064799 1106 58-59 3.5891156665397688 1106 60-61 4.340725120400215 1106 62-63 1.9588435195651215 1106 64-65 1.6902670313547645 1106 66-67 0.6855520490567244 1106 68-69 2.1502224249497495 1106 70-71 2.6405250627822774 1106 72-73 2.11700212835672 1106 74-75 2.2419586110959884 1106 76-77 2.166524461008656 1106 78-79 2.6764808084672858 1106 80-81 2.564782808706454 1106 82-83 3.1406434741171765 1106 84-85 2.699938946292665 1106 86-87 2.4185257240254003 1106 88-89 3.162321712301239 1106 90-91 1.9091212568250633 1106 92-93 2.06359060732105 1106 94-95 0.9337598849899251 1106 96-97 0.8085248699137999 1106 98-99 -0.034153383309138974 1106 100-101 -4.0148329280853545E-4 1107 1 0.9505704829317239 1107 2 0.9998434588284439 1107 3 0.4664784751056317 1107 4 1.0433002462099203 1107 5 1.1167270477620015 1107 6 1.4431084070701203 1107 7 0.8091619566498629 1107 8 0.8353324585074233 1107 9 -0.08874444411502935 1107 10-11 0.4341990892343688 1107 12-13 0.7087261525376007 1107 14-15 1.1169256774141871 1107 16-17 0.36275843803597496 1107 18-19 0.6643425701929644 1107 20-21 0.43947081593434234 1107 22-23 0.2837738914981003 1107 24-25 1.0160612971113565 1107 26-27 0.6818024336192181 1107 28-29 0.3578128612234224 1107 30-31 -0.8122146992048336 1107 32-33 -0.5374870204328595 1107 34-35 -0.8981929709921488 1107 36-37 0.5448495876061266 1107 38-39 0.19748501547199382 1107 40-41 1.4641860023906794 1107 42-43 0.6616062945100758 1107 44-45 -0.3342021345292494 1107 46-47 0.04286249681347698 1107 48-49 0.991955581618587 1107 50-51 0.8575271270384803 1107 52-53 0.56649308439167 1107 54-55 0.6591780088646324 1107 56-57 0.813212662389784 1107 58-59 1.3013995067305864 1107 60-61 1.1388765813423731 1107 62-63 1.16576063345898 1107 64-65 1.293129285379809 1107 66-67 1.2105967717758617 1107 68-69 1.9263106777821903 1107 70-71 1.935396857654073 1107 72-73 1.636978276239848 1107 74-75 1.2392752479475106 1107 76-77 1.3376633995894593 1107 78-79 1.123111696958638 1107 80-81 1.0790940788316767 1107 82-83 0.6358730507421022 1107 84-85 1.075609787079788 1107 86-87 1.2008751575376237 1107 88-89 1.0505149144479304 1107 90-91 1.617827279484576 1107 92-93 1.7872042030276667 1107 94-95 1.6508141485558134 1107 96-97 1.6144282688404594 1107 98-99 1.6199909219979602 1107 100-101 1.9885669126523275 1108 1 -0.7850064401451995 1108 2 -0.4296437206587349 1108 3 -0.4598035761764194 1108 4 -0.0865074460977695 1108 5 0.5654449964799468 1108 6 -0.15464800318629202 1108 7 -0.2421200946321882 1108 8 -0.9707572850823247 1108 9 -0.37560341847400736 1108 10-11 -0.7300637312784524 1108 12-13 -0.8457610269495817 1108 14-15 -0.6755422713037618 1108 16-17 -0.48740181837428764 1108 18-19 -0.3845356349352471 1108 20-21 -1.289695850732322 1108 22-23 -1.2731171341429288 1108 24-25 -1.2715989592988954 1108 26-27 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-0.7837937915432676 2311 66-67 -0.41440322822413833 2311 68-69 -0.5320226555511454 2311 70-71 -0.09985955260233581 2311 72-73 0.22992699418856333 2311 74-75 0.38350601717827715 2311 76-77 0.017150579076634642 2311 78-79 0.08144503029197381 2311 80-81 -0.1805213057837065 2311 82-83 -0.12053720566815329 2311 84-85 0.19099440246440125 2311 86-87 -0.29591971425724495 2311 88-89 -0.19948508555206956 2311 90-91 -0.12576246410516845 2311 92-93 0.1045118953353601 2311 94-95 0.13478850753017468 2311 96-97 -0.05864865423646748 2311 98-99 -1.3351372831302477 2311 100-101 -1.162074112988698 2312 1 0.6481832150272133 2312 2 -0.007229927555286508 2312 3 -0.027332311808603293 2312 4 0.6670845079252175 2312 5 0.9390082148707499 2312 6 1.7340014221010662 2312 7 0.7406385260574666 2312 8 0.5342714505498591 2312 9 0.7529885355489867 2312 10-11 0.8349506365376413 2312 12-13 0.46027345580903756 2312 14-15 0.26303531490313503 2312 16-17 -0.2356058413627906 2312 18-19 -0.9660729912570858 2312 20-21 -1.0806441265943931 2312 22-23 -0.7907177088555741 2312 24-25 0.5240188567930559 2312 26-27 -0.5785866026318871 2312 28-29 -0.30001207246358064 2312 30-31 -0.6603579352791016 2312 32-33 -0.40088224589705135 2312 34-35 -1.4311284263413988 2312 36-37 -1.0811451073540859 2312 38-39 0.05645937444635152 2312 40-41 0.5983593003570853 2312 42-43 0.2982110690458839 2312 44-45 -0.33265483125780904 2312 46-47 -0.6184991300653913 2312 48-49 -0.8553034811577191 2312 50-51 -1.0874330851631129 2312 52-53 -0.4372646521246857 2312 54-55 -1.1330412661132634 2312 56-57 -0.8282550652848428 2312 58-59 -0.3858241891137979 2312 60-61 -0.5834930030961765 2312 62-63 0.30347062461724406 2312 64-65 -0.19626063504459523 2312 66-67 -0.5672546871100792 2312 68-69 -0.7226725229251478 2312 70-71 -0.41119907514875464 2312 72-73 -0.7544894514347753 2312 74-75 -1.3137751499304748 2312 76-77 -1.3900112246368899 2312 78-79 -1.454032423288929 2312 80-81 -1.3937175922558573 2312 82-83 -1.2783621393551563 2312 84-85 -1.2214724410369264 2312 86-87 -1.3042088389256818 2312 88-89 -1.476010284491064 2312 90-91 -2.0223141882430973 2312 92-93 -1.8925040197840026 2312 94-95 -1.5273467709844155 2312 96-97 -1.3550677523797 2312 98-99 -0.7482672566050468 2312 100-101 -0.5006019644475828 2313 1 0.09416022652146694 2313 2 -0.09631038732540276 2313 3 -0.23480357617642156 2313 4 -0.31567411276443735 2313 5 0.5321116631466154 2313 6 -0.18381466985295702 2313 7 -1.125453427965521 2313 8 -0.6749239517489904 2313 9 -0.9797700851406717 2313 10-11 0.022019602054882625 2313 12-13 0.429238973050424 2313 14-15 -0.1401256046370989 2313 16-17 0.17301484829238944 2313 18-19 -0.5157856349352485 2313 20-21 -0.3021958507323248 2313 22-23 -0.5418671341429331 2313 24-25 -0.05701562596556187 2313 26-27 0.18693063874741966 2313 28-29 0.4321718355823947 2313 30-31 -0.48593264792278035 2313 32-33 0.15802580007995815 2313 34-35 0.9556531828540038 2313 36-37 1.018567536324074 2313 38-39 0.38979270777968367 2313 40-41 0.25008343828811874 2313 42-43 -0.5428808849771052 2313 44-45 -1.097022647349764 2313 46-47 -0.8891887852378026 2313 48-49 -1.5426598029967984 2313 50-51 -1.0770882575769072 2313 52-53 -1.2309428130442264 2313 54-55 -0.8517194270328012 2313 56-57 -1.90986426068714 2313 58-59 -1.4723184419873618 2313 60-61 -0.7579182904525013 2313 62-63 0.016401659100004906 2313 64-65 0.17902672127724628 2313 66-67 0.6080326692117595 2313 68-69 0.7250286265001371 2313 70-71 0.5905250627822767 2313 72-73 0.48890135316292316 2313 74-75 0.8347880684603304 2313 76-77 -0.48541352348746614 2313 78-79 0.33016297900991987 2313 80-81 0.983581258318857 2313 82-83 0.578821768690819 2313 84-85 0.537148248618248 2313 86-87 0.41561874728121495 2313 88-89 0.7941046580376714 2313 90-91 0.6075708692281658 2313 92-93 0.6166913825148477 2313 94-95 1.0008141485558113 2313 96-97 0.3676333970455836 2313 98-99 0.9629396399466756 2313 100-101 1.240489989575405 2314 1 0.47011651067447247 2314 2 0.5088808695051981 2314 3 -0.31704401333488974 2314 4 -0.08616591604312518 2314 5 0.19112805658923904 2314 6 -0.7922846152081497 2314 7 -0.27490697987808943 2314 8 -0.14322996267795318 2314 9 -0.8445241835013277 2314 10-11 -0.3890459717156105 2314 12-13 -1.0281380761299097 2314 14-15 -0.42195620572999104 2314 16-17 -0.10403433203548929 2314 18-19 -0.46974738356912127 2314 20-21 -0.3009663425355953 2314 22-23 -0.5424135822303668 2314 24-25 -0.8824254620311365 2314 26-27 -0.7852005087935616 2314 28-29 -0.6705604048547613 2314 30-31 -0.41120587196649794 2314 32-33 -0.5967556206850659 2314 34-35 -1.1760408062170349 2314 36-37 -2.306978911763359 2314 38-39 -1.9107537403077473 2314 40-41 -2.1245067256463095 2314 42-43 -1.5779901745945892 2314 44-45 -1.1858204615574124 2314 46-47 -2.0670576376968235 2314 48-49 -1.480091496985871 2314 50-51 -1.83856366741297 2314 52-53 -1.5549865288912201 2314 54-55 -1.7056811756666832 2314 56-57 -2.91587518964889 2314 58-59 -2.988848496632169 2314 60-61 -2.607235230343207 2314 62-63 -2.3987622753262237 2314 64-65 -2.634361256864832 2314 66-67 -2.5504372761434304 2314 68-69 -2.2096708270517773 2314 70-71 -1.687343789676742 2314 72-73 -1.4117817069463676 2314 74-75 -1.615075319517814 2314 76-77 -1.1537195344164246 2314 78-79 -0.7806293707168557 2314 80-81 -0.7632766651784131 2314 82-83 -0.40300883240207597 2314 84-85 -0.12678617761126176 2314 86-87 -0.0290533838663265 2314 88-89 0.16842159792838274 2314 90-91 0.49718835556696206 2314 92-93 0.682538377050367 2314 94-95 0.27649720866510563 2314 96-97 0.19891755005104983 2314 98-99 0.31334947601224883 2314 100-101 0.07641895132404031 2315 1 1.673647406008648 2315 2 1.7280485870335731 2315 3 1.717760526387682 2315 4 0.3612489641586407 2315 5 1.701342432377384 2315 6 0.23798020194191594 2315 7 0.3886491361370439 2315 8 0.5584093815843438 2315 9 1.5163837610131736 2315 10-11 1.1624042174395015 2315 12-13 1.6061620499734985 2315 14-15 1.8374384979270033 2315 16-17 1.2576302329077649 2315 18-19 0.8617784676288593 2315 20-21 0.4420349184984431 2315 22-23 1.1478764556006595 2315 24-25 1.0160612971113565 2315 26-27 0.4638537156705027 2315 28-29 0.38858209199264593 2315 30-31 0.2954776084874773 2315 32-33 0.7548206718748354 2315 34-35 0.7530890802899037 2315 36-37 0.9371572799138157 2315 38-39 0.694920912907893 2315 40-41 0.9539295921342692 2315 42-43 1.4000678329716116 2315 44-45 1.6324645321374156 2315 46-47 1.3761958301468091 2315 48-49 1.320160709823714 2315 50-51 1.7344502039615541 2315 52-53 1.4793135972121867 2315 54-55 1.8719985216851462 2315 56-57 2.231161380338502 2315 58-59 3.050117455448536 2315 60-61 3.218363760829554 2315 62-63 2.529863197561543 2315 64-65 2.6008215930721192 2315 66-67 2.610596771775864 2315 68-69 2.192977344448856 2315 70-71 2.625140447397662 2315 72-73 2.8933885326501034 2315 74-75 2.4777367864090465 2315 76-77 2.5863813483074054 2315 78-79 2.794906568753511 2315 80-81 2.417555617293214 2315 82-83 2.2794627943318453 2315 84-85 2.9294559409259406 2315 86-87 2.5752341318966003 2315 88-89 3.181284145217159 2315 90-91 3.497314458971754 2315 92-93 3.2487426645661266 2315 94-95 3.1328654306070973 2315 96-97 3.214428268840461 2315 98-99 2.2764011784082143 2315 100-101 2.0167720408574574 2316 1 0.24287817523941513 2316 2 0.1280485870335717 2316 3 -0.4822394736123172 2316 4 -0.8079818050721279 2316 5 -0.929426798391848 2316 6 -0.5774044134427001 2316 7 -0.9959662484783429 2316 8 -1.3646675414925795 2316 9 -1.375923931294519 2316 10-11 -1.0683650133297355 2316 12-13 -1.809222565411119 2316 14-15 -1.4164076559191443 2316 16-17 -1.6577543824768455 2316 18-19 -1.1382215323711407 2316 20-21 -1.7118112353477066 2316 22-23 -1.3675081597839558 2316 24-25 -1.4608617798117187 2316 26-27 -2.051530899714116 2316 28-29 -1.6268025233919658 2316 30-31 -1.942983929974062 2316 32-33 -1.8221024050482448 2316 34-35 -1.3315263043254824 2316 36-37 -1.2859196431631084 2316 38-39 -0.712771394784415 2316 40-41 -0.9537627155580388 2316 42-43 -1.0768552439514636 2316 44-45 -1.7213816217087334 2316 46-47 -1.4468810929301128 2316 48-49 -0.8721469824839794 2316 50-51 0.08060405011540084 2316 52-53 -0.47453255663397087 2316 54-55 -1.2280014783148552 2316 56-57 -1.43806938889227 2316 58-59 -0.7960363907053107 2316 60-61 -1.0739439314781407 2316 62-63 -0.8701368024384557 2316 64-65 -0.9684091761586515 2316 66-67 0.17213523331432512 2316 68-69 0.2545158059873174 2316 70-71 0.17129429355150805 2316 72-73 -0.00661146734989515 2316 74-75 0.893121401793664 2316 76-77 1.0863813483074054 2316 78-79 0.5487527225996658 2316 80-81 0.44832484806244466 2316 82-83 1.125616640485692 2316 84-85 0.7448405563105567 2316 86-87 0.8675418242042916 2316 88-89 1.0505149144479304 2316 90-91 0.9973144589717542 2316 92-93 0.9948965107199754 2316 94-95 1.29440389214556 2316 96-97 1.1682744226866113 2316 98-99 1.8917857937928275 2316 100-101 0.778310502395918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5113.0 3 583.0 4 437.0 5 373.0 6 373.0 7 377.0 8 343.0 9 344.0 10 360.0 11 330.0 12 418.0 13 475.0 14 501.0 15 735.0 16 936.0 17 1332.0 18 1182.0 19 597.0 20 466.0 21 428.0 22 431.0 23 444.0 24 465.0 25 417.0 26 508.0 27 493.0 28 532.0 29 603.0 30 697.0 31 821.0 32 897.0 33 1190.0 34 1619.0 35 3161.0 36 4093.0 37 7216.0 38 8058.0 39 2186.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.249949460235314 20.44232402053936 15.335786196579468 17.971940322645857 2 18.52787773420127 23.345328104152347 37.91695305866656 20.209841102979826 3 20.42330395407132 30.65213875636775 30.500525592302097 18.424031697258833 4 14.493075912261194 22.041847771151318 34.29091276660265 29.17416354998484 5 12.92526632840769 38.56758778224747 31.76534799571449 16.741797893630352 6 25.902421278143823 37.48534702291928 18.67900885241926 17.933222846517644 7 22.932330827067666 38.88147788826906 21.66707090306411 16.51912038159916 8 25.723004789717262 32.66506335765243 20.304763444554474 21.307168408075828 9 28.628592215351034 18.04898751060992 20.138636271775596 33.18378400226345 10-11 24.192489164149247 29.162330642472494 24.867393434838398 21.777786758539865 12-13 27.629345189975744 29.061236863379143 25.505254648342763 17.804163298302345 14-15 25.421193592399817 28.46530901005609 27.021072312901108 19.092425084642983 16-17 19.586420189810088 32.02009278256739 28.029835962846544 20.36365106477598 18-19 19.251061249241967 28.314129775621588 33.44350111178492 18.991307863351526 20-21 22.38190528166879 26.830796596123136 33.3858872516322 17.40141087057587 22-23 22.173825164224358 27.21980798383022 32.87721071248105 17.729156139464376 24-25 20.57961641842323 27.963258624522545 33.210727349891876 18.246397607162347 26-27 20.116411847330713 28.026758556573935 33.98781313473256 17.869016461362786 28-29 20.116621022101402 27.86474386830111 33.86557254454135 18.15306256505614 30-31 20.194824223684076 28.260627924131725 33.668819029718776 17.875728822465415 32-33 19.927452030433773 28.416979054047225 33.55293071567865 18.102638199840353 34-35 20.17644918748484 28.555259115530763 33.275729646697386 17.99256205028701 36-37 19.82698507342018 28.648523006336468 32.85565582965306 18.668836090590293 38-39 19.717057396928052 28.988480194017786 32.883993532740504 18.41046887631366 40-41 20.09640453525738 28.909234220578426 32.919016148264916 18.07534509589927 42-43 20.09902991107518 28.940986257073565 32.41511721907842 18.544866612772836 44-45 20.268617800729654 29.390304292023323 31.867287849541693 18.473790057705326 46-47 20.30237799270331 29.405047044437033 31.120071956260297 19.17250300659936 48-49 20.44813680603984 29.401776780570632 30.58326511223634 19.56682130115319 50-51 20.428292791381594 30.166446018736547 29.685399844367417 19.71986134551445 52-53 20.662476506133668 30.187344637335546 29.195044562559364 19.955134293971426 54-55 20.348367280957 30.002222761073394 29.260628839314577 20.38878111865503 56-57 20.426869321799238 30.311359938556688 28.95920285388012 20.30256788576395 58-59 19.92744469931992 30.06093432633717 29.358623268222196 20.652997706120715 60-61 19.90521327014218 29.77091522751847 29.56173768934609 20.762133812993262 62-63 20.11741643425892 29.681500343559275 29.499616021987794 20.70146720019401 64-65 19.83405089644857 29.444343379216946 29.97594647585552 20.74565924847897 66-67 20.108942810077714 29.375145273923458 29.68843164798739 20.82748026801144 68-69 20.31673202085944 29.8884262440878 29.030399805958684 20.76444192909407 70-71 20.031124944419744 29.588908201624964 29.260479404988075 21.119487448967217 72-73 19.958567025415594 29.57101712899803 29.252690616947095 21.21772522863928 74-75 20.360921096504967 29.614323677111017 28.55136456870333 21.47339065768069 76-77 20.30803124778931 29.11744196622571 28.667724429262968 21.906802356722014 78-79 20.46488125315816 29.72612430520465 28.04345629105609 21.7655381505811 80-81 20.551793835270338 29.65133906013138 28.421424962102073 21.375442142496208 82-83 20.509166616134053 29.707112970711297 28.049643239746935 21.73407717340772 84-85 20.616472966144517 29.5644264780192 27.99191510864073 21.827185447195554 86-87 20.815014705436464 29.465449804432854 27.82511142779176 21.89442406233892 88-89 21.258147642867968 29.32140872113587 27.269971199029865 22.150472436966297 90-91 21.3760902301232 29.16106602524584 27.45510222644446 22.007741518186506 92-93 21.33825238793147 29.129226259665437 27.35432354576237 22.178197806640725 94-95 21.883463357451795 28.756214883382512 27.374792837220586 21.985528921945107 96-97 21.605892516140766 28.714902043991795 27.24783526820446 22.43137017166298 98-99 21.86666397219303 28.89519844798319 26.904656050440543 22.333481529383235 100-101 22.037581748895697 28.62095804146324 27.045112249951984 22.296347959689076 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 733.0 1 445.5 2 145.5 3 123.0 4 120.5 5 133.5 6 154.5 7 225.5 8 338.5 9 379.5 10 289.0 11 194.5 12 169.0 13 173.0 14 174.0 15 173.0 16 185.0 17 177.0 18 185.5 19 202.5 20 212.0 21 236.5 22 247.0 23 272.5 24 302.0 25 327.0 26 408.5 27 489.0 28 557.0 29 663.0 30 768.0 31 880.0 32 1028.0 33 1146.5 34 1171.5 35 1241.5 36 1291.0 37 1325.5 38 1359.0 39 1372.0 40 1337.5 41 1275.5 42 1330.0 43 1394.0 44 1438.0 45 1470.0 46 1414.5 47 1303.0 48 1329.5 49 1379.5 50 1385.5 51 1416.0 52 1385.5 53 1302.5 54 1253.5 55 1188.0 56 1114.5 57 1009.5 58 927.0 59 835.0 60 713.0 61 624.0 62 519.0 63 422.0 64 339.5 65 278.5 66 231.5 67 205.5 68 174.0 69 151.5 70 138.5 71 106.5 72 79.0 73 75.0 74 63.0 75 49.0 76 46.5 77 42.5 78 32.5 79 24.0 80 13.5 81 14.0 82 15.0 83 11.5 84 7.0 85 4.0 86 3.5 87 3.0 88 2.0 89 0.5 90 0.5 91 1.0 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13727944442201317 2 0.13727944442201317 3 0.13324181370371865 4 0.13929825978116042 5 0.1312229983445714 6 0.11305366011224614 7 0.11709129083054065 8 0.10699721403480437 9 0.10497839867565713 10-11 0.09185609884119998 12-13 0.10901602939395164 14-15 0.12415714458755601 16-17 0.12819477530585052 18-19 0.12920418298542416 20-21 0.12314773690798239 22-23 0.11911010618968788 24-25 0.10699721403480437 26-27 0.11002543707352526 28-29 0.116081883150967 30-31 0.108006621714378 32-33 0.09993136027778901 34-35 0.11709129083054065 36-37 0.11810069851011426 38-39 0.10901602939395164 40-41 0.11103484475309888 42-43 0.10901602939395164 44-45 0.11810069851011426 46-47 0.12011951386926151 48-49 0.12617595994670328 50-51 0.11810069851011426 52-53 0.10699721403480437 54-55 0.09286550652077362 56-57 0.116081883150967 58-59 0.11002543707352526 60-61 0.11002543707352526 62-63 0.10497839867565713 64-65 0.12314773690798239 66-67 0.11810069851011426 68-69 0.12112892154883514 70-71 0.11305366011224614 72-73 0.11406306779181977 74-75 0.09993136027778901 76-77 0.11810069851011426 78-79 0.11911010618968788 80-81 0.11911010618968788 82-83 0.12314773690798239 84-85 0.11911010618968788 86-87 0.12617595994670328 88-89 0.11406306779181977 90-91 0.12213832922840877 92-93 0.1342512213832923 94-95 0.11305366011224614 96-97 0.09589372955949449 98-99 0.10094076795736262 100-101 0.13828885210158678 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 49534.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.35838010255581 #Duplication Level Percentage of deduplicated Percentage of total 1 84.07175938842698 60.83296321718416 2 10.111042910551866 14.632373723099285 3 3.3201272250432456 7.207170832155691 4 1.2052898833770438 3.4885129406064523 5 0.4770939121700798 1.7260871320709008 6 0.2678421963060097 1.1628376468688175 7 0.14787121254394286 0.7489804982436306 8 0.09486077785837845 0.5491177776880527 9 0.06417052619831483 0.41789477934348124 >10 0.2120417387422577 2.5719707675535997 >50 0.008370068634562803 0.3209916421044131 >100 0.011160091512750405 1.974401421246013 >500 0.008370068634562803 4.366697621835507 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 801 1.617071102676949 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 692 1.3970202285298987 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 670 1.3526062906286591 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 371 0.7489804982436306 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 340 0.6863972221100658 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 164 0.33108571890014943 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 103 0.207937981992167 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55 0.11103484475309888 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 0.10699721403480437 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 51 0.10295958331650987 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0020188153591472525 0.0 6 0.0 0.0 0.0 0.0020188153591472525 0.0 7 0.0 0.0 0.0 0.0020188153591472525 0.0 8 0.0 0.0 0.0 0.0020188153591472525 0.0 9 0.0 0.0 0.0 0.0020188153591472525 0.0 10-11 0.0 0.0 0.0 0.0020188153591472525 0.0 12-13 0.0 0.0 0.0 0.0020188153591472525 0.0 14-15 0.0 0.0 0.0 0.0020188153591472525 0.0 16-17 0.0 0.0 0.0 0.0020188153591472525 0.0 18-19 0.0 0.0 0.0 0.003028223038720879 0.0 20-21 0.0 0.0 0.0 0.007065853757015383 0.0 22-23 0.0 0.0 0.0 0.015141115193604393 0.0 24-25 0.0 0.0 0.0 0.022206968950619778 0.0 26-27 0.0 0.0 0.0 0.03331045342592967 0.0 28-29 0.0 0.0 0.0 0.056526830056123065 0.0 30-31 0.0 0.0 0.0 0.09791254491864174 0.0 32-33 0.0 0.0 0.0 0.1655428594500747 0.0 34-35 0.0 0.0 0.0 0.2695118504461582 0.0 36-37 0.0 0.0 0.0 0.39165017967456695 0.0 38-39 0.0 0.0 0.0 0.4956191706706504 0.0 40-41 0.0 0.0 0.0 0.5975693463075867 0.0 42-43 0.0 0.0 0.0 0.6813501837121977 0.0 44-45 0.0 0.0 0.0 0.756046352000646 0.0 46-47 0.0 0.0 0.0 0.8630435660354504 0.0 48-49 0.0 0.0 0.0 0.9791254491864174 0.0 50-51 0.0 0.0 0.0 1.0972261476965317 0.0 52-53 0.0 0.0 0.0 1.1779787620624218 0.0 54-55 0.0 0.0 0.0 1.301126498970404 0.0 56-57 0.0 0.0 0.0 1.4515282432268743 0.0 58-59 0.0 0.0 0.0 1.5686195340574152 0.0 60-61 0.0 0.0 0.0 1.6998425324019866 0.0 62-63 0.0 0.0 0.0 1.8169338232325272 0.0 64-65 0.0 0.0 0.0 1.9582508983728348 0.0 66-67 0.0 0.0 0.0 2.138934873016514 0.0 68-69 0.0 0.0 0.0 2.3075059555053095 0.0 70-71 0.0 0.0 0.0 2.4770864456736787 0.0 72-73 0.0 0.0 0.0 2.6688739047926675 0.0 74-75 0.0 0.0 0.0 2.878830702143982 0.0 76-77 0.0 0.0 0.0 3.105947430048048 0.0 78-79 0.0 0.0 0.0 3.333064157952114 0.0 80-81 0.0 0.0 0.0 3.556143255137885 0.0 82-83 0.0 0.0 0.0 3.8004199135947028 0.0 84-85 0.0 0.0 0.0 3.999273226470707 0.0 86-87 0.0 0.0 0.0 4.246578107966245 0.0 88-89 0.0 0.0 0.0 4.5917955343804255 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGTAAA 25 0.0049929507 57.0 6 GGTATCA 430 0.0 47.5 1 GTATAAA 55 0.0025558984 34.545456 1 GTATCAA 635 0.0 32.16535 2 GAGCAAT 35 0.008355147 27.142855 10-11 TATCAAC 790 0.0 25.85443 3 ATCAACG 805 0.0 24.782608 4 TCAACGC 810 0.0 24.62963 4 AACGCAG 840 0.0 23.75 7 CAACGCA 850 0.0 23.470589 6 GAGTACT 525 0.0 22.166666 12-13 GTGGTAT 200 5.1743336E-7 21.375 1 GTACTTT 545 0.0 21.35321 14-15 ACGCAGA 945 0.0 21.111113 8 CGCAGAG 950 0.0 21.0 9 TGGTATC 210 8.2707265E-7 20.357143 2 ACTTTTT 615 0.0 19.308943 16-17 GTACATG 495 0.0 19.191921 1 TACATGG 495 0.0 19.191921 2 CAGAGTA 955 0.0 19.149216 10-11 >>END_MODULE