FastQCFastQC Report
Fri 10 Feb 2017
SRR2031760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49534
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT36517.370694876246618No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT14322.8909435942988653TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT11222.265110832963217RNA PCR Primer, Index 1 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10102.039003512738725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9761.9703637905277183No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG7211.455565873945169TruSeq Adapter, Index 1 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5441.0982355553761054No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT4920.9932571567004481TruSeq Adapter, Index 1 (96% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.9872007106230065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.975087818468123No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG3210.648039730286268TruSeq Adapter, Index 1 (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.5026850244276658No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.3008034885129406No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC1480.29878467315379337No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA1280.2584083659708483RNA PCR Primer, Index 1 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT1200.2422578430976703No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC1120.2261073202244923Illumina PCR Primer Index 5 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC1120.2261073202244923No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.18573101304154724No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.16756167480922193No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG610.12314773690798239No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.11103484475309888No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGT550.11103484475309888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG520.10497839867565713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC6400.062.3391081
TATACAC6950.058.0892033
TGAGTCC250.004994481356.9957543
TACTGTG250.004994481356.9957547
GAGATGT250.004994481356.9957541
TTATACA7050.056.5915342
GGTATCA5050.052.669351
ATACACA8800.045.8772664
TACACAT8700.045.8586545
ACACATC9900.039.820276
CACATCT10250.038.4605527
ACATCTC10550.037.3668868
GTATCAA7850.035.697981
CATCTCC11400.034.5807579
GTGGTAT2000.033.2475241
TGGTATC2000.033.2475242
GGGGTGG753.0377257E-431.664317
TGGGGTG600.00391627731.664316
TATCAAC9000.031.136572
ATCAACG9050.030.9645463
Produced by FastQC (version 0.11.5)