FastQCFastQC Report
Fri 10 Feb 2017
SRR2031757_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031757_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132595
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24260.2141983674658638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23140.2043095722654612No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14330.12652360287657988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG2050.046.3442041
GGTATCA23350.045.7735821
CGTCAGA2250.042.2247162
GTATCAA37750.032.8430021
TCAACGC41050.029.971324
ATCAACG40950.029.9285073
CAACGCA42100.029.4494865
AACGCAG44100.028.3255836
TATCAAC43900.028.0255742
TGTCGCG350.00838721927.14686212-13
ACGCAGA49050.025.563877
CGCAGAG48950.025.2290888
GTGGTAT13150.024.2029511
TGGTATC13250.023.6617742
GAGTACT28400.022.66619712-13
GCAGAGT53100.022.2733579
CAGAGTA50100.020.48161110-11
GTACATG33200.019.8882241
ACATGGG31500.019.1519263
GTACTTT33300.019.11693614-15
Produced by FastQC (version 0.11.5)