FastQCFastQC Report
Fri 10 Feb 2017
SRR2031757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132595
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT45520.40190889064493485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36980.3265068272418649No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36720.3242112140703429No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT17190.15177534776332227TruSeq Adapter, Index 1 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15990.1411802100486052No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT11630.10268454301846644RNA PCR Primer, Index 1 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11470.10127185798983748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25100.051.297091
GTATCAA41100.034.3331
ATCAACG49000.028.9852073
TCAACGC49150.028.8967484
CAACGCA50150.028.3180395
TATCAAC50850.028.210922
AACGCAG51700.027.5609176
GTGGTAT12450.027.476431
GAACGCG350.00839434727.14213666-67
CGCAGAG56850.024.9806448
ACGCAGA56900.024.875227
TGGTATC13850.024.6935792
GAGTACT41200.022.31122412-13
GTACATG29750.022.198571
GCAGAGT63550.022.048019
TACATGG30550.020.9905512
CAGAGTA61650.020.80512410-11
CCTAGAC3201.8189894E-1120.7816163
AACCGCA1150.003724769720.6507157
ACATGGG28650.020.5588263
Produced by FastQC (version 0.11.5)