##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031757_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1132595 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7399140910917 34.0 31.0 34.0 31.0 34.0 2 32.90851186876156 34.0 31.0 34.0 31.0 34.0 3 32.96100812735356 34.0 31.0 34.0 31.0 34.0 4 36.36280665198063 37.0 37.0 37.0 35.0 37.0 5 36.33551710894009 37.0 37.0 37.0 35.0 37.0 6 36.36257797359162 37.0 37.0 37.0 35.0 37.0 7 36.31431888715737 37.0 37.0 37.0 35.0 37.0 8 36.31207889845885 37.0 37.0 37.0 35.0 37.0 9 38.156893682207674 39.0 39.0 39.0 37.0 39.0 10-11 38.12453259991436 39.0 39.0 39.0 37.0 39.0 12-13 38.0764699649919 39.0 39.0 39.0 35.0 39.0 14-15 39.55825559886809 41.0 39.0 41.0 37.0 41.0 16-17 39.53451321964162 41.0 39.0 41.0 37.0 41.0 18-19 39.55433407352143 41.0 39.5 41.0 37.0 41.0 20-21 39.564663891329204 41.0 40.0 41.0 37.0 41.0 22-23 39.47692114127292 41.0 39.0 41.0 36.0 41.0 24-25 39.4146848608726 41.0 39.0 41.0 36.0 41.0 26-27 39.28416998132607 41.0 39.0 41.0 36.0 41.0 28-29 39.18484233110688 41.0 39.0 41.0 36.0 41.0 30-31 39.02185379592882 40.0 39.0 41.0 35.5 41.0 32-33 38.81914188213793 40.0 38.0 41.0 35.0 41.0 34-35 38.67359338510235 40.0 38.0 41.0 35.0 41.0 36-37 38.566744952962004 40.0 38.0 41.0 35.0 41.0 38-39 38.615401357060556 40.0 38.0 41.0 35.0 41.0 40-41 38.551977538308044 40.0 38.0 41.0 35.0 41.0 42-43 38.45677007226767 40.0 38.0 41.0 35.0 41.0 44-45 38.27628278422561 40.0 38.0 41.0 34.0 41.0 46-47 38.07088985912881 40.0 38.0 41.0 34.0 41.0 48-49 37.84134708346761 40.0 38.0 41.0 33.0 41.0 50-51 37.69391971534397 40.0 38.0 41.0 33.0 41.0 52-53 37.77226810995987 40.0 38.0 41.0 33.0 41.0 54-55 37.720120166520246 40.0 38.0 41.0 33.0 41.0 56-57 37.56879069746908 40.0 38.0 41.0 33.0 41.0 58-59 37.42382051836711 40.0 37.0 41.0 33.0 41.0 60-61 37.182379844516355 40.0 37.0 41.0 32.5 41.0 62-63 36.937825966033756 40.0 36.5 41.0 32.0 41.0 64-65 36.64404707772858 39.0 36.0 41.0 31.5 41.0 66-67 36.29957354570698 39.0 35.5 41.0 31.0 41.0 68-69 35.913650952017264 39.0 35.0 41.0 31.0 41.0 70-71 35.583652585434336 38.0 35.0 40.0 30.0 41.0 72-73 35.20554037409666 37.0 35.0 40.0 30.0 41.0 74-75 34.82412159686384 37.0 35.0 39.0 29.5 41.0 76-77 33.85644162299851 36.0 34.0 39.0 28.5 39.5 78-79 34.02723126978311 36.0 34.0 38.5 29.0 39.5 80-81 33.750400187180766 35.5 34.0 37.0 29.0 39.0 82-83 33.419653980460794 35.0 34.0 37.0 29.0 39.0 84-85 33.06846533844843 35.0 34.0 36.5 29.0 38.0 86-87 32.76943037890861 35.0 34.0 36.0 29.0 37.0 88-89 32.498997876557816 35.0 34.0 36.0 28.0 37.0 90-91 32.26425862731162 35.0 34.0 35.5 27.0 36.5 92-93 32.073132055147695 35.0 34.0 35.0 27.0 36.0 94-95 31.872878213306613 35.0 34.0 35.0 26.0 36.0 96-97 31.697599318379474 35.0 34.0 35.0 26.0 36.0 98-99 31.495692193590827 35.0 34.0 35.0 25.0 35.5 100-101 30.834887581174208 34.5 32.5 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1580843325116703 1101 2 0.1749436361107115 1101 3 0.22972213955694087 1101 4 0.16156718323678376 1101 5 0.13475148660599245 1101 6 0.17999022942290566 1101 7 0.1972876972062565 1101 8 0.1472604301014755 1101 9 0.23079454368699004 1101 10-11 0.15124017174035487 1101 12-13 0.19347454676831433 1101 14-15 0.17195344038145777 1101 16-17 0.1210585293459232 1101 18-19 0.19606519193222027 1101 20-21 0.18269453192291252 1101 22-23 0.08404502269750225 1101 24-25 0.12535356101832207 1101 26-27 0.18932246673121256 1101 28-29 0.13029269797017662 1101 30-31 0.15278716570828266 1101 32-33 0.25766225811617716 1101 34-35 -0.019210988690886666 1101 36-37 0.11736237176435083 1101 38-39 0.19714987327949274 1101 40-41 0.15799414597906747 1101 42-43 0.26183177125155055 1101 44-45 0.39597894408273504 1101 46-47 0.292949717476624 1101 48-49 0.040854097955211444 1101 50-51 0.057085262155119665 1101 52-53 -0.07484833962282522 1101 54-55 0.038347097672676966 1101 56-57 -0.054522360684892135 1101 58-59 0.022735207361328946 1101 60-61 0.04440298408572119 1101 62-63 0.06660866317448466 1101 64-65 0.17643699127685153 1101 66-67 -0.14543574033389461 1101 68-69 -0.16657461048836097 1101 70-71 0.034681750832710634 1101 72-73 -0.016233983598574753 1101 74-75 -0.060349159412453446 1101 76-77 -0.20589155133366432 1101 78-79 -0.20121197521049794 1101 80-81 -0.1215247233648995 1101 82-83 -0.03520118328722788 1101 84-85 0.00854000955237666 1101 86-87 0.06439599517724304 1101 88-89 0.11254139366909044 1101 90-91 0.029810659720808985 1101 92-93 -0.07599388619488678 1101 94-95 -0.0850612933147481 1101 96-97 -0.09774205629421573 1101 98-99 -0.22623217621604752 1101 100-101 -0.3535298229015389 1102 1 0.12591568448905122 1102 2 0.04280202593256632 1102 3 0.028370034437209313 1102 4 -0.05881040984152719 1102 5 0.03615170631508846 1102 6 0.09416636288341351 1102 7 0.1991154129129029 1102 8 0.12757709222635327 1102 9 0.08917691416080942 1102 10-11 0.09589721690846176 1102 12-13 0.09576999589458524 1102 14-15 0.2772977790400404 1102 16-17 0.20263511244365162 1102 18-19 0.1157601326618547 1102 20-21 0.1276858244596184 1102 22-23 0.09197434960420026 1102 24-25 0.22676729964965148 1102 26-27 0.1319117554868754 1102 28-29 0.17501797643868855 1102 30-31 -0.023843407919812876 1102 32-33 0.05826879999715828 1102 34-35 0.14965791395584205 1102 36-37 0.14318510995286005 1102 38-39 -0.03702340736908383 1102 40-41 0.12167934031091221 1102 42-43 0.06177939873392546 1102 44-45 -0.285909638708425 1102 46-47 -0.2858097138344746 1102 48-49 -0.13081386028302688 1102 50-51 -0.03740774632667154 1102 52-53 -0.03308924043013661 1102 54-55 -0.10361968197101845 1102 56-57 0.0388226038891446 1102 58-59 -0.18058565733873877 1102 60-61 -0.1991180458219759 1102 62-63 -0.1653169635925522 1102 64-65 -0.17092798981794743 1102 66-67 -0.14863557342310685 1102 68-69 -0.11929084937516876 1102 70-71 -0.08694490550138312 1102 72-73 -0.1049724020337024 1102 74-75 0.011202131591389275 1102 76-77 0.03344393509205901 1102 78-79 0.053260446188147625 1102 80-81 -0.24051252865835693 1102 82-83 -0.06338973286653982 1102 84-85 -0.05503245612872121 1102 86-87 -0.01374497205375036 1102 88-89 -0.10610215812435086 1102 90-91 -0.22640059313200567 1102 92-93 -0.1576699983657477 1102 94-95 -0.08085552467353452 1102 96-97 -0.24339673405420115 1102 98-99 -0.28479306033227303 1102 100-101 -0.2960461946357107 1103 1 0.06620369143595184 1103 2 0.09086851195835521 1103 3 0.03817429102229397 1103 4 0.07020978763057428 1103 5 -9.838627840608183E-4 1103 6 0.06904113373215637 1103 7 0.09250279110091952 1103 8 0.13351391884327768 1103 9 0.15800973022874132 1103 10-11 0.15455441064982267 1103 12-13 0.05805451331008271 1103 14-15 0.08278956979680174 1103 16-17 0.14868104721997355 1103 18-19 0.1897041272908666 1103 20-21 0.23190592625101658 1103 22-23 0.09686190468089961 1103 24-25 -0.039878830508264684 1103 26-27 -0.006152881248077335 1103 28-29 0.16261968743473432 1103 30-31 0.08689192639980803 1103 32-33 -0.07991845276070109 1103 34-35 -0.3193659619969438 1103 36-37 -0.19699445216723888 1103 38-39 -0.3065012641214011 1103 40-41 -0.2928723674689664 1103 42-43 -0.23443076110912386 1103 44-45 -0.2232804898646279 1103 46-47 -0.28525442405864254 1103 48-49 0.024137615933483403 1103 50-51 -0.1788131671609463 1103 52-53 -0.2676853512910995 1103 54-55 -0.20875689131074182 1103 56-57 -0.1903593060262665 1103 58-59 -0.12856379952508235 1103 60-61 -0.27429825117366136 1103 62-63 -0.2860429911550213 1103 64-65 -0.11408742280929829 1103 66-67 -0.12654257821518655 1103 68-69 -0.16750055493590565 1103 70-71 -0.3019661723460132 1103 72-73 -0.2776851106819507 1103 74-75 -0.14816754164695567 1103 76-77 -9.383012633890075E-4 1103 78-79 -0.02299214683922912 1103 80-81 -0.17325864514759814 1103 82-83 -0.10621991168492428 1103 84-85 -0.36876313907986713 1103 86-87 -0.3375844415484863 1103 88-89 -0.18415931729200707 1103 90-91 -0.3540388477230678 1103 92-93 -0.35979296414582507 1103 94-95 -0.5124870414136389 1103 96-97 -0.4115098342696868 1103 98-99 -0.19798744894064058 1103 100-101 -0.16506564837127868 1104 1 0.0757417439450947 1104 2 0.047249369386072715 1104 3 0.125994164734486 1104 4 0.02045903987384179 1104 5 0.0686904476180672 1104 6 0.006678222465950512 1104 7 0.11862442619563751 1104 8 0.05910381581901447 1104 9 0.10139623737396875 1104 10-11 0.14414397528923217 1104 12-13 0.22623331025258864 1104 14-15 0.22149844317665668 1104 16-17 0.13946702262708044 1104 18-19 0.1491507873108091 1104 20-21 0.09897970491502406 1104 22-23 0.15541125662507227 1104 24-25 0.07828334961802597 1104 26-27 0.22782019950964383 1104 28-29 0.28355687586942935 1104 30-31 0.25154795830409427 1104 32-33 0.270314182666354 1104 34-35 0.3687696312233939 1104 36-37 0.380074341451909 1104 38-39 0.29408032444622734 1104 40-41 0.39095116194279456 1104 42-43 0.2504811697649174 1104 44-45 0.2959867085401484 1104 46-47 0.36344115586825154 1104 48-49 0.4426404407922462 1104 50-51 0.48596749086497226 1104 52-53 0.4143352535610134 1104 54-55 0.4246179840631328 1104 56-57 0.3373658518334821 1104 58-59 0.3334501586005345 1104 60-61 0.48984265942787175 1104 62-63 0.2980547157243336 1104 64-65 0.32419772175700245 1104 66-67 0.2585355208210416 1104 68-69 0.3600833599859925 1104 70-71 0.4332282444037361 1104 72-73 0.3988187111857826 1104 74-75 0.32169786214167573 1104 76-77 0.31244654420022044 1104 78-79 0.347380069844057 1104 80-81 0.34467089955163743 1104 82-83 0.2791822152675536 1104 84-85 0.36169548504775406 1104 86-87 0.29345577114676047 1104 88-89 0.33269844276116345 1104 90-91 0.22523998245306842 1104 92-93 0.20405715217188458 1104 94-95 0.23902508886220275 1104 96-97 0.2286363937130318 1104 98-99 0.32825050386760424 1104 100-101 0.23425306216378416 1105 1 0.12853338491034094 1105 2 0.07339471789725849 1105 3 0.05710035363521371 1105 4 0.07978164403466792 1105 5 0.05997720064148382 1105 6 -0.047824665438497505 1105 7 0.028723680387301442 1105 8 0.09693442947534692 1105 9 0.01068145765845685 1105 10-11 0.0836769572141236 1105 12-13 0.18674565627774342 1105 14-15 0.18749547115849197 1105 16-17 0.14529844003675407 1105 18-19 0.015573991482852989 1105 20-21 0.12074132662758075 1105 22-23 0.23288139625385185 1105 24-25 0.26529525638263607 1105 26-27 0.20645951987076927 1105 28-29 0.3656911213693448 1105 30-31 0.2156790635584045 1105 32-33 0.046499718844593474 1105 34-35 -0.003697143856520313 1105 36-37 0.08170614765388962 1105 38-39 0.10199916405070297 1105 40-41 0.036460891096616876 1105 42-43 -0.03841394977003176 1105 44-45 0.08020276865342879 1105 46-47 -0.04876608165021423 1105 48-49 -0.01678917471458874 1105 50-51 -0.016417133146696017 1105 52-53 0.0479321432225035 1105 54-55 -0.05979434140088813 1105 56-57 -0.27251797777081066 1105 58-59 -0.06916858193667252 1105 60-61 -0.09998705701542576 1105 62-63 -0.17866540988548962 1105 64-65 0.011204955537074568 1105 66-67 0.07821065493569535 1105 68-69 -0.04243818247273623 1105 70-71 -0.055354719763812454 1105 72-73 -0.20876460812859676 1105 74-75 -0.10170397714503565 1105 76-77 -0.11931339523790285 1105 78-79 -0.020700641944948472 1105 80-81 -0.11156099263325814 1105 82-83 0.04002961428435725 1105 84-85 -0.025376235918997736 1105 86-87 -0.08053813528139386 1105 88-89 0.06002091607842175 1105 90-91 -0.0445136578018932 1105 92-93 -0.01372962888944329 1105 94-95 -0.05869636387523158 1105 96-97 -0.1457014443774014 1105 98-99 -0.26154463249008586 1105 100-101 -0.0817911629619168 1106 1 0.10634298918252227 1106 2 0.0629031190375926 1106 3 0.0435808202497725 1106 4 0.03922123669569544 1106 5 0.016887641187437907 1106 6 0.06688173515064477 1106 7 0.0778103754568562 1106 8 0.0973437563449906 1106 9 0.017180086458623123 1106 10-11 0.040187558599335205 1106 12-13 0.03194374608568751 1106 14-15 0.12333145126140721 1106 16-17 0.10372917298484907 1106 18-19 0.16043458993138415 1106 20-21 0.24367582981047775 1106 22-23 0.1334081899955777 1106 24-25 0.11667662161877246 1106 26-27 0.11151827589660002 1106 28-29 0.0760575728306705 1106 30-31 0.2136193271359943 1106 32-33 0.23281302106426693 1106 34-35 0.12500046478904636 1106 36-37 0.23563609221856296 1106 38-39 0.15599371539502727 1106 40-41 0.2177493219650941 1106 42-43 0.2841211660477896 1106 44-45 0.21451890814984864 1106 46-47 0.10639952404964248 1106 48-49 0.11061042499445506 1106 50-51 0.21000568434141798 1106 52-53 0.25455456403401655 1106 54-55 0.1842290796862187 1106 56-57 0.33328398211877186 1106 58-59 0.35053964842490615 1106 60-61 0.34499552626181185 1106 62-63 0.2818991539735691 1106 64-65 0.4561464254090808 1106 66-67 0.38686931879520614 1106 68-69 0.5871440420787337 1106 70-71 0.5780289134865413 1106 72-73 0.5693883608465953 1106 74-75 0.5770435549211612 1106 76-77 0.4830278098818539 1106 78-79 0.42897564646332853 1106 80-81 0.4696732227558016 1106 82-83 0.5042193865341602 1106 84-85 0.6246032073783923 1106 86-87 0.6619902939606206 1106 88-89 0.7762887224749448 1106 90-91 0.6740876731881329 1106 92-93 0.6939070731829489 1106 94-95 0.7730553834444898 1106 96-97 0.6875900225579343 1106 98-99 0.6764012333537046 1106 100-101 0.8038701981176928 1107 1 0.3082715754495595 1107 2 0.21995729047500134 1107 3 0.16717363346387515 1107 4 0.18950023433327345 1107 5 0.14343710469067616 1107 6 0.16368787648046634 1107 7 0.18069794061669597 1107 8 0.11437154982392883 1107 9 0.20024794931256906 1107 10-11 0.20361060597820568 1107 12-13 0.21995820082043593 1107 14-15 0.31204980133460225 1107 16-17 0.2837930404960005 1107 18-19 0.23740556934595247 1107 20-21 0.2316279629950131 1107 22-23 0.27606994823627673 1107 24-25 0.1986797038783763 1107 26-27 0.2995762107826536 1107 28-29 0.3171540456241786 1107 30-31 0.2645688853293393 1107 32-33 0.2576590288617169 1107 34-35 0.37028069436424715 1107 36-37 0.4318702280022464 1107 38-39 0.4734621077405876 1107 40-41 0.5281985685506996 1107 42-43 0.6021451381045466 1107 44-45 0.4589251866837074 1107 46-47 0.5408396687902339 1107 48-49 0.6786496151041703 1107 50-51 0.821236124312243 1107 52-53 0.7658912888191551 1107 54-55 0.7617754464364666 1107 56-57 0.6972012538842165 1107 58-59 0.6969088142340425 1107 60-61 0.601499432228735 1107 62-63 0.7471073031201527 1107 64-65 0.671568351966279 1107 66-67 0.8542601715375966 1107 68-69 0.801049960693561 1107 70-71 0.8642949830257933 1107 72-73 0.7163638332101243 1107 74-75 0.5696606252285648 1107 76-77 0.573184386737843 1107 78-79 0.6220487752467108 1107 80-81 0.6336407382516427 1107 82-83 0.5099626314095502 1107 84-85 0.519556611149568 1107 86-87 0.5961540981839946 1107 88-89 0.5241642339706161 1107 90-91 0.4178615570767974 1107 92-93 0.4699847770444663 1107 94-95 0.5225353125235337 1107 96-97 0.6058565172364467 1107 98-99 0.7034169007015656 1107 100-101 0.7937871510286918 1108 1 0.04377364587104893 1108 2 0.06017905280473457 1108 3 -0.043589299201705956 1108 4 0.010669049277247211 1108 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-0.5145135515860417 2316 54-55 -0.591470404417251 2316 56-57 -0.7419233638527913 2316 58-59 -0.6627240141935786 2316 60-61 -0.6378151224348727 2316 62-63 -0.5398129313344882 2316 64-65 -0.6292298211351479 2316 66-67 -0.6079664706005303 2316 68-69 -0.4886834905042008 2316 70-71 -0.41554243574331906 2316 72-73 -0.7061253758240227 2316 74-75 -0.5134009430053794 2316 76-77 -0.8469744116821332 2316 78-79 -0.6915522218226116 2316 80-81 -0.441159370056198 2316 82-83 -0.3021750151246039 2316 84-85 -0.3994334977953855 2316 86-87 -0.353273701892455 2316 88-89 -0.3933585425329511 2316 90-91 -0.3955244170567376 2316 92-93 -0.23995326449477972 2316 94-95 -0.2898083976574064 2316 96-97 -0.3495690290810849 2316 98-99 -0.3240970757892576 2316 100-101 -0.25957736063033465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 11.0 5 10.0 6 14.0 7 55.0 8 171.0 9 354.0 10 652.0 11 1057.0 12 1598.0 13 2243.0 14 2879.0 15 3689.0 16 4636.0 17 6611.0 18 6847.0 19 4150.0 20 3241.0 21 3361.0 22 3804.0 23 4125.0 24 4707.0 25 5486.0 26 6079.0 27 5704.0 28 6846.0 29 9154.0 30 11774.0 31 15332.0 32 19439.0 33 25998.0 34 36589.0 35 56116.0 36 93136.0 37 202857.0 38 408465.0 39 175270.0 40 132.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.21578497449592 22.29045681262628 16.112341570938067 22.381416641939722 2 14.82206790600347 22.804091489014166 41.24175014016484 21.132090464817523 3 17.000859984919362 27.231237053871787 33.582057924487316 22.18584503672153 4 12.495407918162037 20.54532738011134 39.74173072032102 27.2175339814056 5 12.325500289158967 37.99778385036134 36.591544197175516 13.085171663304182 6 26.552209748409627 39.25807548152694 20.176673921392908 14.01304084867053 7 22.93388192601945 34.64080275826752 25.19435455745434 17.23096075825869 8 22.2926112158362 36.045718019239004 23.397948957924058 18.26372180700074 9 23.900511656858807 17.97217893421744 23.748471430652614 34.378837978271136 10-11 21.89403263658469 27.796809801217382 31.17705903439361 19.132098527804313 12-13 22.87590384370032 26.526110206353827 30.517966066377745 20.080019883568113 14-15 20.566751574923074 28.53747367770474 28.351308278775733 22.544466468596454 16-17 19.536878761336986 31.0985752832481 29.59134751221993 19.773198443194982 18-19 19.28829716928448 29.34870277818123 31.92553197594724 19.437468076587056 20-21 20.143828994477282 28.391481509277366 31.787973635765653 19.676715860479696 22-23 20.010462698493285 28.76646109156406 31.24846039405083 19.974615815891823 24-25 19.890605203095546 28.983396536272892 31.562694520106483 19.56330374052508 26-27 19.69393228367661 29.143050126148633 31.781907438023826 19.381110152150928 28-29 19.495523114204847 28.619840632490078 32.26289237593921 19.621743877365866 30-31 20.129975909294988 28.522697223057325 31.75805639176245 19.589270475885236 32-33 19.901553294764003 28.700172392100427 31.52409185121745 19.87418246191812 34-35 19.688766873619713 29.100111880034934 31.733001431393344 19.47811981495201 36-37 19.919694317456884 28.87861215534006 31.490334842467977 19.711358684735078 38-39 19.546837130660123 28.693354641332512 31.757380175614408 20.002428052392958 40-41 19.635792141056598 28.606077194407533 31.861653989290083 19.896476675245786 42-43 19.92707013539703 28.611286470450604 31.425134315443735 20.03650907870863 44-45 19.89501984381001 28.94106013182117 31.149572442046804 20.01434758232201 46-47 20.048781779894842 28.786900878072036 30.85012736238461 20.314189979648507 48-49 19.960709697641256 28.720769560169344 30.928972845544966 20.38954789664443 50-51 19.838909760329155 28.99778826500205 30.798652651653946 20.364649323014845 52-53 19.939342836583247 29.07857619007677 30.941775303616915 20.040305669723068 54-55 20.012184408371926 29.115438440042556 30.383234960422744 20.489142191162774 56-57 20.159456822606494 29.476202879228673 29.996909751499874 20.36743054666496 58-59 19.773113153821637 29.541334868456488 30.612462927923673 20.073089049798206 60-61 20.12116599423351 29.722796256506722 30.13243673617325 20.02360101308651 62-63 19.808555108066894 29.93373910478917 30.091665025744092 20.16604076139985 64-65 19.73672302366782 29.858423949393043 30.089270302139866 20.31558272479927 66-67 19.893901780562288 29.78895584288528 30.00100217609073 20.316140200461707 68-69 19.746475664736515 29.78754213927106 30.080729042500405 20.38525315349202 70-71 19.900325977933804 29.522987978018563 30.254495882012232 20.322190162035405 72-73 19.95335126111859 29.475680713532327 30.04154943040062 20.529418594948464 74-75 19.943515271494462 29.625343868554904 29.77496614845585 20.656174711494778 76-77 19.70539647333991 29.636014632169406 30.04914473504136 20.609444159449325 78-79 19.79646668567912 29.699070538261385 29.911283066879395 20.593179709180102 80-81 19.976494605768096 29.434810941398986 29.860018401668516 20.728676051164406 82-83 19.98926250827277 29.526336215060656 29.67344691586301 20.810954360803564 84-85 20.075200384257453 29.379802114475524 29.719470869011648 20.825526632255375 86-87 19.84616457732563 29.473129349605415 29.800834205976827 20.879871867092128 88-89 19.92980705770169 29.25703640100036 29.790811787999655 21.0223447532983 90-91 20.108909186425862 29.335199254808646 29.65164070122153 20.90425085754396 92-93 20.10842357594727 29.182717564531014 29.801782631920503 20.907076227601216 94-95 20.104715277747122 29.121486497821376 29.723555198460172 21.050243025971334 96-97 20.12976406373334 29.043328853940597 29.62394291334759 21.20296416897847 98-99 20.000653367979496 28.978768189455522 29.63593276666905 21.384645675895932 100-101 19.937550023773017 29.260899719359184 29.45766173268002 21.343888524187776 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1517.0 1 905.5 2 296.5 3 290.0 4 228.0 5 211.0 6 343.0 7 623.5 8 1014.0 9 1234.5 10 979.5 11 659.0 12 629.5 13 686.5 14 769.5 15 866.0 16 936.0 17 989.5 18 1104.0 19 1226.0 20 1350.5 21 1497.5 22 1732.5 23 2132.5 24 2648.5 25 3414.5 26 4894.5 27 7481.0 28 11241.5 29 16443.5 30 23224.5 31 31340.0 32 39690.5 33 46629.5 34 52573.5 35 55829.5 36 57960.5 37 59188.5 38 55404.5 39 50428.0 40 47052.0 41 43738.5 42 41217.0 43 40174.0 44 38959.5 45 37605.5 46 36997.0 47 36184.0 48 34851.0 49 32965.5 50 29857.5 51 26625.5 52 23955.0 53 21178.5 54 18558.5 55 15869.5 56 13348.0 57 11084.5 58 9216.0 59 7781.5 60 6307.0 61 4952.5 62 3790.5 63 2781.5 64 1960.5 65 1456.5 66 1063.0 67 780.5 68 634.0 69 508.5 70 375.5 71 270.5 72 197.0 73 142.5 74 91.0 75 64.0 76 50.0 77 32.5 78 20.0 79 13.5 80 8.5 81 5.0 82 4.5 83 4.0 84 3.0 85 1.5 86 0.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020042468843673156 2 0.0 3 0.001500977842918254 4 0.018629783815044213 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.829281428930906E-5 12-13 1.324392214339636E-4 14-15 0.0 16-17 0.0016775634714968723 18-19 1.324392214339636E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.2073203572327266E-4 28-29 0.004900251193056653 30-31 4.414640714465453E-5 32-33 2.2073203572327266E-4 34-35 0.011654651486188796 36-37 0.00419390867874218 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.5317125715723626E-4 60-61 0.0014568314357735995 62-63 0.009005867057509524 64-65 0.002074881135798763 66-67 0.005253422450213889 68-69 0.0036200053858616715 70-71 2.648784428679272E-4 72-73 0.007813914064603852 74-75 0.006754400293132143 76-77 0.0019424419143647994 78-79 3.5317125715723626E-4 80-81 0.007858060471748507 82-83 0.010109527236125888 84-85 0.0018541491000754902 86-87 0.0018541491000754902 88-89 2.2073203572327266E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 8.829281428930906E-5 98-99 1.7658562857861813E-4 100-101 7.50488921459127E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1132595.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.85414174549867 #Duplication Level Percentage of deduplicated Percentage of total 1 58.79968406215363 26.374093635143787 2 19.887799497133095 17.8410035530093 3 8.798281944268656 11.83918156335264 4 4.505875504278838 8.084287142259733 5 2.4183270718392618 5.4235992633627506 6 1.49697482782353 4.028731266998404 7 0.9656021245211829 3.0317878194119454 8 0.6805261745350258 2.441953399529282 9 0.4484398669361536 1.810294682029812 >10 1.9132084454166796 13.92275502702013 >50 0.06273761685420355 1.871936293605338 >100 0.02036767773803426 1.4581711375296502 >500 3.954884548530929E-4 0.13215774933453217 >1k 0.001779698046838918 1.7400474674126525 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 4552 0.40190889064493485 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3698 0.3265068272418649 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3672 0.3242112140703429 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 1719 0.15177534776332227 TruSeq Adapter, Index 1 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1599 0.1411802100486052 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 1163 0.10268454301846644 RNA PCR Primer, Index 1 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1147 0.10127185798983748 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.829281428930906E-5 0.0 2 0.0 0.0 0.0 2.648784428679272E-4 0.0 3 0.0 0.0 0.0 2.648784428679272E-4 0.0 4 0.0 0.0 0.0 2.648784428679272E-4 0.0 5 0.0 0.0 0.0 2.648784428679272E-4 0.0 6 0.0 0.0 0.0 2.648784428679272E-4 0.0 7 0.0 0.0 0.0 2.648784428679272E-4 0.0 8 0.0 0.0 0.0 2.648784428679272E-4 0.0 9 0.0 0.0 0.0 2.648784428679272E-4 0.0 10-11 0.0 2.648784428679272E-4 0.0 3.5317125715723626E-4 0.0 12-13 0.0 2.648784428679272E-4 0.0 3.973176643018908E-4 0.0 14-15 0.0 3.090248500125817E-4 0.0 4.414640714465453E-4 0.0 16-17 0.0 3.5317125715723626E-4 0.0 4.414640714465453E-4 0.0 18-19 0.0 3.5317125715723626E-4 0.0 4.414640714465453E-4 0.0 20-21 0.0 3.5317125715723626E-4 0.0 6.180497000251635E-4 0.0 22-23 0.0 3.5317125715723626E-4 0.0 9.270745500377451E-4 8.829281428930906E-5 24-25 0.0 4.414640714465453E-4 0.0 0.0016334170643522179 1.7658562857861813E-4 26-27 0.0 5.297568857358544E-4 0.0 0.002957809278691854 1.7658562857861813E-4 28-29 0.0 5.297568857358544E-4 0.0 0.004326347900176144 1.7658562857861813E-4 30-31 0.0 5.297568857358544E-4 0.0 0.00847611017177367 1.7658562857861813E-4 32-33 0.0 5.297568857358544E-4 0.0 0.01977759040080523 1.7658562857861813E-4 34-35 0.0 5.297568857358544E-4 0.0 0.04008493768734632 1.7658562857861813E-4 36-37 0.0 5.739032928805089E-4 0.0 0.06644034275270506 1.7658562857861813E-4 38-39 0.0 6.180497000251635E-4 0.0 0.0924425765609066 1.7658562857861813E-4 40-41 0.0 6.180497000251635E-4 0.0 0.11614919719758607 1.7658562857861813E-4 42-43 0.0 6.180497000251635E-4 0.0 0.14811119597031597 1.7658562857861813E-4 44-45 0.0 6.180497000251635E-4 0.0 0.1908890644934862 1.7658562857861813E-4 46-47 0.0 6.180497000251635E-4 0.0 0.23768425606682 1.7658562857861813E-4 48-49 0.0 6.180497000251635E-4 0.0 0.2811243206971601 1.7658562857861813E-4 50-51 0.0 6.180497000251635E-4 0.0 0.32213633293454413 1.7658562857861813E-4 52-53 0.0 7.063425143144725E-4 0.0 0.3659737152291861 1.7658562857861813E-4 54-55 0.0 9.712209571823997E-4 0.0 0.4228784340386458 1.7658562857861813E-4 56-57 0.0 9.712209571823997E-4 0.0 0.49607317708448295 1.7658562857861813E-4 58-59 0.0 0.001412685028628945 0.0 0.5627342518729114 1.7658562857861813E-4 60-61 0.0 0.001412685028628945 0.0 0.629218741032761 1.7658562857861813E-4 62-63 0.0 0.001412685028628945 0.0 0.7044442188072524 1.7658562857861813E-4 64-65 0.0 0.0015892706572075634 0.0 0.7969750881824482 1.7658562857861813E-4 66-67 0.0 0.0016775634714968723 0.0 0.9152874593301225 1.7658562857861813E-4 68-69 0.0 0.0016775634714968723 0.0 1.0352773939492934 1.7658562857861813E-4 70-71 0.0 0.0016775634714968723 0.0 1.1503229309682632 2.648784428679272E-4 72-73 0.0 0.0016775634714968723 0.0 1.2772438515091449 2.648784428679272E-4 74-75 0.0 0.0016775634714968723 0.0 1.4255757795151842 2.648784428679272E-4 76-77 0.0 0.0016775634714968723 0.0 1.59350871229345 2.648784428679272E-4 78-79 0.0 0.0016775634714968723 0.0 1.77751093727237 2.648784428679272E-4 80-81 0.0 0.0016775634714968723 0.0 1.956966082315391 2.648784428679272E-4 82-83 0.0 0.0016775634714968723 0.0 2.150901248901858 2.648784428679272E-4 84-85 0.0 0.0016775634714968723 0.0 2.373134262468049 2.648784428679272E-4 86-87 0.0 0.0016775634714968723 0.0 2.633068307735775 2.648784428679272E-4 88-89 0.0 0.0016775634714968723 0.0 2.940636326312583 2.648784428679272E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2510 0.0 51.29709 1 GTATCAA 4110 0.0 34.333 1 ATCAACG 4900 0.0 28.985207 3 TCAACGC 4915 0.0 28.896748 4 CAACGCA 5015 0.0 28.318039 5 TATCAAC 5085 0.0 28.21092 2 AACGCAG 5170 0.0 27.560917 6 GTGGTAT 1245 0.0 27.47643 1 GAACGCG 35 0.008394347 27.142136 66-67 CGCAGAG 5685 0.0 24.980644 8 ACGCAGA 5690 0.0 24.87522 7 TGGTATC 1385 0.0 24.693579 2 GAGTACT 4120 0.0 22.311224 12-13 GTACATG 2975 0.0 22.19857 1 GCAGAGT 6355 0.0 22.04801 9 TACATGG 3055 0.0 20.990551 2 CAGAGTA 6165 0.0 20.805124 10-11 CCTAGAC 320 1.8189894E-11 20.781616 3 AACCGCA 115 0.0037247697 20.650715 7 ACATGGG 2865 0.0 20.558826 3 >>END_MODULE