FastQCFastQC Report
Fri 10 Feb 2017
SRR2031755_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031755_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3536690
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38450.039.7841031
GTATCAA57650.035.0218961
TCAACGC61900.031.6169344
ATCAACG63950.030.5295623
CAACGCA65050.030.083355
AACGCAG67900.029.166286
TATCAAC69050.028.3454652
ACGCAGA76050.025.3562267
CGCAGAG77650.024.2866928
GTACATG84200.020.1421661
ACATGGG83350.018.6363743
GCAGAGT95950.018.5159479
TACATGG89650.017.910862
CATGGGG47850.017.4720524
GAGTACA69500.016.5417181
GTGGTAT28750.016.3586481
CAGAGTA87500.016.2601110-11
AGAGTAC73900.015.78126210-11
GAGTACT34000.015.50958812-13
AGTACAT68200.015.3245732
Produced by FastQC (version 0.11.5)