##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031755_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3536690 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52187101498859 34.0 31.0 34.0 31.0 34.0 2 32.70821785341661 34.0 31.0 34.0 31.0 34.0 3 32.77437293062157 34.0 31.0 34.0 31.0 34.0 4 36.23150007492882 37.0 37.0 37.0 35.0 37.0 5 36.17845047205155 37.0 37.0 37.0 35.0 37.0 6 36.20216360495265 37.0 37.0 37.0 35.0 37.0 7 36.1495265346977 37.0 37.0 37.0 35.0 37.0 8 36.13702049091099 37.0 37.0 37.0 35.0 37.0 9 37.957266822933306 39.0 38.0 39.0 35.0 39.0 10-11 37.90266477977997 39.0 38.0 39.0 35.0 39.0 12-13 37.851362432104594 39.0 38.0 39.0 35.0 39.0 14-15 39.293355793128605 41.0 39.0 41.0 36.0 41.0 16-17 39.264610271185774 41.0 39.0 41.0 36.0 41.0 18-19 39.25108872985758 41.0 39.0 41.0 36.0 41.0 20-21 39.23865153575801 41.0 39.0 41.0 36.0 41.0 22-23 39.14427275220616 40.0 39.0 41.0 36.0 41.0 24-25 39.07758242876814 40.0 39.0 41.0 36.0 41.0 26-27 38.942949339636776 40.0 38.5 41.0 35.0 41.0 28-29 38.846251297116794 40.0 38.0 41.0 35.0 41.0 30-31 38.701873644565964 40.0 38.0 41.0 35.0 41.0 32-33 38.54223482974193 40.0 38.0 41.0 34.0 41.0 34-35 38.4147252091645 40.0 38.0 41.0 34.0 41.0 36-37 38.33881567228114 40.0 38.0 41.0 34.0 41.0 38-39 38.451692825777776 40.0 38.0 41.0 34.0 41.0 40-41 38.41216914685765 40.0 38.0 41.0 34.0 41.0 42-43 38.35650749712302 40.0 38.0 41.0 34.0 41.0 44-45 38.236086425443 40.0 38.0 41.0 34.0 41.0 46-47 38.121301838724904 40.0 38.0 41.0 33.0 41.0 48-49 37.97723210120197 40.0 38.0 41.0 33.0 41.0 50-51 37.87759684903116 40.0 37.5 41.0 33.0 41.0 52-53 37.985920026917825 40.0 38.0 41.0 33.0 41.0 54-55 37.94398010569205 40.0 37.0 41.0 33.0 41.0 56-57 37.78609151494759 40.0 37.0 41.0 33.0 41.0 58-59 37.63330204230509 40.0 37.0 41.0 33.0 41.0 60-61 37.40449148780357 40.0 36.0 41.0 32.5 41.0 62-63 37.17006042372954 39.0 36.0 41.0 32.0 41.0 64-65 36.87057842219703 39.0 35.0 41.0 31.5 41.0 66-67 36.5485565599473 39.0 35.0 40.5 31.0 41.0 68-69 36.18973107623229 38.0 35.0 40.0 31.0 41.0 70-71 35.86350330393673 37.0 35.0 40.0 31.0 41.0 72-73 35.49059897814058 37.0 35.0 39.5 30.0 41.0 74-75 35.10547065193727 36.0 34.5 39.0 30.0 41.0 76-77 34.114436945279344 35.0 33.5 38.0 28.5 39.0 78-79 34.364261922871385 35.5 34.0 37.5 30.0 39.0 80-81 34.11509405687239 35.0 34.0 37.0 30.0 39.0 82-83 33.79677381958837 35.0 34.0 37.0 29.5 39.0 84-85 33.44358199333275 35.0 34.0 36.0 29.0 37.0 86-87 33.1424678159522 35.0 34.0 36.0 29.0 37.0 88-89 32.86745756625545 35.0 34.0 35.5 29.0 36.5 90-91 32.622404564720114 35.0 33.5 35.0 29.0 36.0 92-93 32.41611860242204 35.0 33.0 35.0 28.5 36.0 94-95 32.2012605006376 35.0 33.0 35.0 27.0 36.0 96-97 32.00086055040165 35.0 33.0 35.0 27.0 35.5 98-99 31.746804356615932 35.0 33.0 35.0 26.5 35.0 100-101 30.941759526562972 34.5 32.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17848683194241488 1101 2 0.1632981727466074 1101 3 0.193017098692863 1101 4 0.1682508576284647 1101 5 0.13821629376161582 1101 6 0.10998444421284148 1101 7 0.1235906626476222 1101 8 0.16969739862176425 1101 9 0.08230021194613357 1101 10-11 0.11054038486523865 1101 12-13 0.10768756013871439 1101 14-15 0.07372045360383339 1101 16-17 0.08234366745804778 1101 18-19 0.1375262907645478 1101 20-21 0.13511281456203506 1101 22-23 0.024767353949826543 1101 24-25 0.036304090481742435 1101 26-27 0.09930845734219673 1101 28-29 0.09809849166989437 1101 30-31 0.1307501387269312 1101 32-33 0.11807133576489548 1101 34-35 -0.19508434613128856 1101 36-37 0.0472313530234274 1101 38-39 -0.0019389825357336576 1101 40-41 -0.2693247467019617 1101 42-43 -0.14144975522302872 1101 44-45 -0.11419250036330197 1101 46-47 -0.10277926687699335 1101 48-49 -0.2362442744272215 1101 50-51 -0.11671758188920478 1101 52-53 -0.006357630936541625 1101 54-55 -0.056731410002143434 1101 56-57 -0.1738459468048461 1101 58-59 -0.13271830552309893 1101 60-61 -0.0745359437780948 1101 62-63 0.042500772752823934 1101 64-65 0.06220491668012329 1101 66-67 -0.21883904251738784 1101 68-69 -0.3028624764713612 1101 70-71 -0.1382437581485476 1101 72-73 -0.2237988662778747 1101 74-75 -0.16306093648191222 1101 76-77 -0.20974655513600027 1101 78-79 -0.27429636997170803 1101 80-81 -0.31675820603435056 1101 82-83 -0.16530978796854612 1101 84-85 -0.07616707582231186 1101 86-87 -0.05247647393190391 1101 88-89 -0.08092263270363986 1101 90-91 -0.17847272689122207 1101 92-93 -0.3219650517813477 1101 94-95 -0.22805787381612674 1101 96-97 -0.07306100124279169 1101 98-99 -0.45199057113286045 1101 100-101 -0.6420326975412252 1102 1 0.18326344222531077 1102 2 0.12663243226313625 1102 3 0.13610888722822523 1102 4 0.06013023455289357 1102 5 0.16209061259167612 1102 6 0.1264620371950187 1102 7 0.1587583679625908 1102 8 0.11285530529652021 1102 9 0.1687248990757766 1102 10-11 0.2157592948799305 1102 12-13 0.17414819829784278 1102 14-15 0.2928661913654622 1102 16-17 0.2563451191084454 1102 18-19 0.27935321547719383 1102 20-21 0.26169298705893596 1102 22-23 0.21644310288235147 1102 24-25 0.21888712091536178 1102 26-27 0.20950448685152168 1102 28-29 0.21714681008034376 1102 30-31 0.054305661519542525 1102 32-33 0.20805937099009952 1102 34-35 0.18797999713866176 1102 36-37 0.3059779436296637 1102 38-39 0.1449831216768871 1102 40-41 0.1478345549181057 1102 42-43 0.2673522929649579 1102 44-45 0.14156023140458984 1102 46-47 0.10660769435373396 1102 48-49 0.20072994176662462 1102 50-51 0.13752061352789013 1102 52-53 0.17644076544340237 1102 54-55 0.3388135543019928 1102 56-57 0.3436461003057474 1102 58-59 0.30332520996589807 1102 60-61 0.39478268781457615 1102 62-63 0.2911708681874998 1102 64-65 0.30298438489975865 1102 66-67 0.3006361518393277 1102 68-69 0.4389628037935651 1102 70-71 0.31442552073277596 1102 72-73 0.3870811400731 1102 74-75 0.4176750956358575 1102 76-77 0.3934320488979992 1102 78-79 0.3370243483684163 1102 80-81 0.10969069417035371 1102 82-83 0.2542552272567775 1102 84-85 0.38683931484175815 1102 86-87 0.3620015840320434 1102 88-89 0.1841164087534537 1102 90-91 0.12229443632305248 1102 92-93 0.35424415599178616 1102 94-95 0.381187211269868 1102 96-97 0.10433237900455339 1102 98-99 0.20978537570044153 1102 100-101 0.2509301436107947 1103 1 0.22529508598619685 1103 2 0.13567454600401163 1103 3 0.16999784828405495 1103 4 0.1312112664824383 1103 5 0.10800887919945268 1103 6 0.1319895631101673 1103 7 0.1593231753201536 1103 8 0.14361470359742157 1103 9 0.19367410798175655 1103 10-11 0.15197659243001027 1103 12-13 0.058188623332270595 1103 14-15 0.21504877950294343 1103 16-17 0.2131602187421322 1103 18-19 0.24689056186985425 1103 20-21 0.312932497593863 1103 22-23 0.21823546992919773 1103 24-25 0.05826255932954183 1103 26-27 0.17217939786969083 1103 28-29 0.2508094844717519 1103 30-31 0.23997659758251189 1103 32-33 0.1154746109163085 1103 34-35 0.07041796965172153 1103 36-37 0.029972643689411882 1103 38-39 -0.11007286543698314 1103 40-41 -0.15646671031272774 1103 42-43 -0.0157255693924796 1103 44-45 0.10072860842779363 1103 46-47 -0.010619709454950055 1103 48-49 0.13070474107035324 1103 50-51 -0.04571598723592274 1103 52-53 -0.14669139532145437 1103 54-55 0.04293529704705179 1103 56-57 -0.04651367730460265 1103 58-59 0.10413293125139234 1103 60-61 -0.019997650446306636 1103 62-63 -0.11904242842729928 1103 64-65 0.04015671089803874 1103 66-67 0.12258751709353533 1103 68-69 0.03481300254132691 1103 70-71 0.10924560854551402 1103 72-73 0.21781040145886976 1103 74-75 0.3052077656106533 1103 76-77 0.2803030057450471 1103 78-79 0.20795989174219187 1103 80-81 0.11988554491468051 1103 82-83 0.22326330706763997 1103 84-85 0.1588083465634611 1103 86-87 0.15498643763417874 1103 88-89 0.258622137365343 1103 90-91 0.07954184480815485 1103 92-93 0.1509922269694215 1103 94-95 0.11615694445424651 1103 96-97 -0.011240092482289299 1103 98-99 0.11093239708206326 1103 100-101 0.06733683608435825 1104 1 0.21228513050829179 1104 2 0.09722889859977357 1104 3 0.14901585739719536 1104 4 0.07977882165302219 1104 5 0.14270769282136087 1104 6 0.1517366235034885 1104 7 0.16546204710554946 1104 8 0.18968004608066735 1104 9 0.0959012069784535 1104 10-11 0.17514920589577088 1104 12-13 0.1712483735930448 1104 14-15 0.28171345835392714 1104 16-17 0.22317746801272165 1104 18-19 0.27475606029724275 1104 20-21 0.32270877390818953 1104 22-23 0.21537853153309072 1104 24-25 0.25588006284176146 1104 26-27 0.3132488128903148 1104 28-29 0.29837423632527305 1104 30-31 0.2861489342449488 1104 32-33 0.272494905023585 1104 34-35 0.2720465373460357 1104 36-37 0.32012555144135035 1104 38-39 0.23713164451923774 1104 40-41 0.29140009013394064 1104 42-43 0.3916892812803212 1104 44-45 0.31083704874288287 1104 46-47 0.26008918040465545 1104 48-49 0.20855982831309916 1104 50-51 0.33854647599569887 1104 52-53 0.30408641210790677 1104 54-55 0.30165328513904655 1104 56-57 0.28732565451103653 1104 58-59 0.25431663026826357 1104 60-61 0.5028077637672652 1104 62-63 0.43950669472236115 1104 64-65 0.4014231780874056 1104 66-67 0.3437730106110308 1104 68-69 0.42590937731216627 1104 70-71 0.4355991246684354 1104 72-73 0.34626871621855315 1104 74-75 0.3872939185362938 1104 76-77 0.3737776528953418 1104 78-79 0.3949793062410265 1104 80-81 0.3790463686456178 1104 82-83 0.38738144494154625 1104 84-85 0.44854445906189966 1104 86-87 0.359290008894142 1104 88-89 0.34775216486179517 1104 90-91 0.32515565495882726 1104 92-93 0.36671823440683227 1104 94-95 0.3696006611925 1104 96-97 0.3552358432695186 1104 98-99 0.4794564787236446 1104 100-101 0.5946356579556991 1105 1 0.10410103150083927 1105 2 0.10604667564843595 1105 3 0.03034840123189042 1105 4 0.06803631099607088 1105 5 0.07015441946371936 1105 6 0.057708246552635956 1105 7 0.14275513195757128 1105 8 0.1502891334606815 1105 9 -0.008335398815354722 1105 10-11 0.060899182612395464 1105 12-13 0.1665485496922514 1105 14-15 0.1199468383679374 1105 16-17 0.1863918724325515 1105 18-19 0.1482078583006583 1105 20-21 0.08910860028684908 1105 22-23 0.18924760748075897 1105 24-25 0.18852674311673923 1105 26-27 0.18494688149524308 1105 28-29 0.2735805042381614 1105 30-31 0.26078700237708574 1105 32-33 0.16257245646133356 1105 34-35 0.16193938825355048 1105 36-37 0.3054356646284546 1105 38-39 0.17480012857076588 1105 40-41 0.10194889707103982 1105 42-43 0.08024246235890331 1105 44-45 0.22926862367589962 1105 46-47 0.24581397974753827 1105 48-49 0.22227409189342495 1105 50-51 0.23151971022587503 1105 52-53 0.34893204262311883 1105 54-55 0.21531554206275416 1105 56-57 0.13321435172149876 1105 58-59 0.10908832645711186 1105 60-61 0.1672164415307762 1105 62-63 0.1024812020618171 1105 64-65 0.024072020084865642 1105 66-67 0.25199179971036756 1105 68-69 0.1600693487626188 1105 70-71 0.09482664638606053 1105 72-73 -0.016471020655110635 1105 74-75 0.16827668485181846 1105 76-77 0.09943727018217174 1105 78-79 0.19060425337281117 1105 80-81 0.12462857974878716 1105 82-83 0.20461863260610613 1105 84-85 0.3394848482118178 1105 86-87 0.25511435285124406 1105 88-89 0.17372496133605608 1105 90-91 0.17152454506671688 1105 92-93 0.1702851619327319 1105 94-95 0.10594642172434732 1105 96-97 0.23580252785085776 1105 98-99 0.1567111709288227 1105 100-101 0.14097113962534635 1106 1 0.12022438395758428 1106 2 0.06582666942388471 1106 3 0.06625583767698373 1106 4 0.09724032293790685 1106 5 0.061247075043908694 1106 6 0.08014087408538728 1106 7 0.06985046699674768 1106 8 0.0764561037386784 1106 9 0.034761726976356044 1106 10-11 0.0563781363579281 1106 12-13 0.09131909713093478 1106 14-15 0.17249503289226453 1106 16-17 0.14250527606382235 1106 18-19 0.2475391021823512 1106 20-21 0.25013382864429445 1106 22-23 0.1800798702653097 1106 24-25 0.20658447424553827 1106 26-27 0.11538338328342945 1106 28-29 0.07995624302046878 1106 30-31 0.12028665703440566 1106 32-33 0.16263933477713266 1106 34-35 0.16165375835760898 1106 36-37 0.1830794424168758 1106 38-39 0.12360117252144676 1106 40-41 0.24007492700386734 1106 42-43 0.1225524159523772 1106 44-45 0.09320103980287797 1106 46-47 0.039170078747993387 1106 48-49 0.05871285041916963 1106 50-51 0.08839475151782494 1106 52-53 0.24708132493856283 1106 54-55 0.14141996038499371 1106 56-57 0.1299322217607113 1106 58-59 0.15492629147435366 1106 60-61 0.21455394096857816 1106 62-63 0.0980694865311662 1106 64-65 0.2486927582934797 1106 66-67 0.2387420967194842 1106 68-69 0.23970673128955156 1106 70-71 0.06903964769288251 1106 72-73 0.13368458860139043 1106 74-75 0.22531388352647497 1106 76-77 0.17790464271251238 1106 78-79 0.09585196587752876 1106 80-81 0.1701335953070071 1106 82-83 0.1048460549328496 1106 84-85 0.18584340954207335 1106 86-87 0.1404417911584801 1106 88-89 0.22867689953184112 1106 90-91 0.13374685922408247 1106 92-93 0.2219066140809005 1106 94-95 0.31484467177480013 1106 96-97 0.3648094921188161 1106 98-99 0.3734628460638909 1106 100-101 0.4708306311680772 1107 1 0.22543504111822443 1107 2 0.19213911270784223 1107 3 0.21911537294562322 1107 4 0.12590060173069872 1107 5 0.1271382558720262 1107 6 0.14982058987784086 1107 7 0.13662062565691713 1107 8 0.16274869858855112 1107 9 0.2470979135063729 1107 10-11 0.23127558187162833 1107 12-13 0.25065629635732023 1107 14-15 0.2970624868732372 1107 16-17 0.2457757883991718 1107 18-19 0.2756942115463019 1107 20-21 0.25111238812966974 1107 22-23 0.35165307084322706 1107 24-25 0.3356331497765481 1107 26-27 0.3581782121665924 1107 28-29 0.3289678998262602 1107 30-31 0.39171677499622604 1107 32-33 0.4007461404381587 1107 34-35 0.3961925043435741 1107 36-37 0.37799100853357004 1107 38-39 0.43989518282645435 1107 40-41 0.44708929458068525 1107 42-43 0.46614005058918195 1107 44-45 0.4666574652383346 1107 46-47 0.5235022352386807 1107 48-49 0.5094574637448375 1107 50-51 0.5389402015368461 1107 52-53 0.5542072692314974 1107 54-55 0.5178938702275815 1107 56-57 0.4696243843168162 1107 58-59 0.4696500047233201 1107 60-61 0.4434677852041986 1107 62-63 0.5188437470878853 1107 64-65 0.45323649184918935 1107 66-67 0.517924872714616 1107 68-69 0.31592884854450176 1107 70-71 0.3892534596870618 1107 72-73 0.25740781459808915 1107 74-75 0.33042293156642444 1107 76-77 0.3926321091336433 1107 78-79 0.49977462488119073 1107 80-81 0.5939015293168097 1107 82-83 0.5608953244646457 1107 84-85 0.556094833413546 1107 86-87 0.5657733740173398 1107 88-89 0.5789231613770838 1107 90-91 0.5207977363017662 1107 92-93 0.5836246635290294 1107 94-95 0.6138787580277878 1107 96-97 0.7041392095190986 1107 98-99 0.7223416518912664 1107 100-101 0.7695588999004883 1108 1 0.04386723758897659 1108 2 -0.027267319934331624 1108 3 0.006145759877881574 1108 4 0.0422638097341661 1108 5 0.07224071404871779 1108 6 0.03029102138787465 1108 7 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64-65 -0.6085416087879523 2316 66-67 -0.48363739993075683 2316 68-69 -0.6248453765414297 2316 70-71 -0.4512731420011775 2316 72-73 -0.7176990339068539 2316 74-75 -0.598324220413474 2316 76-77 -0.9345002114877232 2316 78-79 -0.6554217014602415 2316 80-81 -0.45650889118455495 2316 82-83 -0.3896776315857835 2316 84-85 -0.39026963992487396 2316 86-87 -0.4378257896686364 2316 88-89 -0.5415830376739663 2316 90-91 -0.6046827154498757 2316 92-93 -0.4665206575960319 2316 94-95 -0.38489296769722614 2316 96-97 -0.48047037959648264 2316 98-99 -0.5093502816402697 2316 100-101 -0.22785164050302598 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 13.0 5 27.0 6 58.0 7 161.0 8 421.0 9 903.0 10 1454.0 11 2255.0 12 2906.0 13 3953.0 14 4735.0 15 5109.0 16 5947.0 17 6853.0 18 7148.0 19 6713.0 20 7023.0 21 8140.0 22 9427.0 23 11093.0 24 14201.0 25 16524.0 26 18323.0 27 22643.0 28 29529.0 29 38328.0 30 49179.0 31 62550.0 32 79287.0 33 105758.0 34 145854.0 35 218780.0 36 373548.0 37 733720.0 38 1172748.0 39 371302.0 40 71.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.46736884019199 22.30372832196666 15.28548093532138 22.943421902519976 2 14.979090618629284 22.99310937628121 40.411062320983746 21.61673768410576 3 17.826202394312553 27.134382976341044 32.19510319284087 22.84431143650553 4 13.059924775883037 19.63765165012302 39.11071519470603 28.191708379287917 5 12.908849800236943 38.0400600561542 36.261334750854616 12.789755392754243 6 28.0833773952481 38.925803505537665 19.388382923015588 13.602436176198648 7 24.22287506114474 33.557337510497106 24.773672558239486 17.44611487011867 8 22.873336368186074 36.294077230404696 23.007416539193425 17.8251698622158 9 24.24012282671085 17.50913990199876 22.90907600044109 35.34166127084929 10-11 22.57230493614365 27.503373212451876 30.84931438679352 19.07500746461096 12-13 23.123909644328457 25.60105635495336 30.106427196050547 21.168606804667643 14-15 20.95400824811119 27.91464388226444 27.107518799012126 24.02382907061224 16-17 20.802960315134335 30.363192517892283 28.209912175376108 20.623934991597277 18-19 20.846207916794377 29.430506089885327 29.399361096439492 20.323924896880808 20-21 21.428298776539645 28.755333376688373 28.994794567802096 20.821573278969886 22-23 21.492666872131853 28.862269523198247 28.489632961893747 21.155430642776153 24-25 21.35164518235978 29.159143153626697 28.657954188803654 20.83125747520987 26-27 21.127853138894583 29.082301879590293 28.93060572430319 20.85923925721193 28-29 21.13209425150427 28.61697379327935 29.169268685725065 21.081663269491322 30-31 21.48681195513995 28.839185135923778 28.64134467049398 21.032658238442295 32-33 21.715584671345344 28.679396954802428 28.515684256168246 21.089334117683983 34-35 21.556524795600414 28.706671571936237 28.543649594085174 21.19315403837817 36-37 21.450496478005544 28.808165784760142 28.711631340215632 21.029706397018685 38-39 21.383157698299822 28.461965849424182 28.87193958192547 21.282936870350525 40-41 21.3446188385185 28.398163254342336 28.919554724898138 21.33766318224102 42-43 21.69715185667955 28.242565788915623 28.706799295386364 21.353483059018462 44-45 21.444896216518835 28.503091874040415 28.517370761927115 21.534641147513636 46-47 21.588731271329973 28.320760371986236 28.535466778258765 21.555041578425023 48-49 21.489358694146222 28.265963372531942 28.620065654609256 21.624612278712583 50-51 21.339232446157283 28.44331847009492 28.632803553605207 21.58464553014259 52-53 21.459825429992453 28.345769632057095 28.749466308893346 21.444938629057113 54-55 21.493557535435677 28.19856984920929 28.62631443524878 21.681558180106258 56-57 21.552666476281495 28.396905015706775 28.38059032598277 21.669838182028958 58-59 21.305396955731634 28.508694173262544 28.747944045812467 21.437964825193355 60-61 21.449908323756187 28.653898110079634 28.490946396577193 21.405247169586982 62-63 21.263336013095866 28.851315030074513 28.385952539642904 21.499396417186713 64-65 21.34586828964916 28.71213206181671 28.293327817703727 21.6486718308304 66-67 21.288221198401654 28.697591187759887 28.289211291672316 21.724976322166142 68-69 21.31032583401054 28.609639352754325 28.38440801383029 21.69562679940485 70-71 21.372231340930846 28.44525311131752 28.396605861138326 21.785909686613312 72-73 21.394253200536813 28.395775636388933 28.28951051877624 21.92046064429802 74-75 21.29931890940108 28.43839200239901 28.28614938580538 21.976139702394526 76-77 21.42726671486702 28.361303015556917 28.291617973849092 21.919812295726974 78-79 21.344211534250174 28.384580377815265 28.281955751948406 21.989252335986155 80-81 21.301593019849406 28.453541256884062 28.21089528756135 22.03397043570518 82-83 21.43827473992944 28.327387462998548 28.17191633855039 22.062421458521616 84-85 21.476729819741657 28.245681574079875 28.053195446441283 22.22439315973719 86-87 21.256257202312547 28.168893813861214 28.373255345598608 22.20159363822764 88-89 21.281567052471274 28.008373959970346 28.325083595396585 22.384975392161795 90-91 21.358459463509664 28.037274966140657 28.217075853410954 22.387189716938718 92-93 21.260104787244572 27.975154735077147 28.371330255125553 22.393410222552728 94-95 21.236806166217566 27.869801989996297 28.352527363155943 22.540864480630194 96-97 21.276255842682236 27.773024430477207 28.339415764292536 22.61130396254802 98-99 21.246403414720213 27.726350189782078 28.198246495025852 22.828999900471857 100-101 21.106786881750892 27.7803555387412 28.31084177985959 22.802015799648313 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1080.0 1 664.5 2 252.5 3 251.5 4 205.0 5 215.0 6 335.5 7 577.0 8 875.0 9 972.5 10 748.0 11 503.5 12 451.0 13 434.0 14 435.5 15 478.5 16 526.0 17 555.5 18 582.0 19 615.5 20 648.5 21 699.5 22 817.5 23 981.0 24 1145.5 25 1361.0 26 1874.5 27 2786.5 28 4756.5 29 9347.5 30 18360.0 31 33398.5 32 56599.5 33 83901.0 34 114338.0 35 148570.5 36 184303.0 37 210773.5 38 221206.0 39 219803.5 40 207958.0 41 192441.5 42 175144.5 43 157752.0 44 141161.5 45 124772.5 46 110764.0 47 99326.5 48 91582.5 49 85039.0 50 78668.0 51 74997.5 52 73191.5 53 71946.5 54 69983.0 55 66648.5 56 61569.0 57 55985.5 58 51753.0 59 46595.5 60 39881.5 61 33173.0 62 26631.5 63 20136.0 64 14535.5 65 10587.5 66 7709.0 67 5874.0 68 4339.0 69 3089.5 70 2334.5 71 1634.0 72 1166.0 73 797.0 74 532.5 75 390.0 76 280.0 77 172.0 78 97.0 79 53.5 80 33.5 81 22.0 82 18.5 83 15.5 84 7.5 85 4.0 86 3.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01758706587232695 2 0.0 3 0.0016682265055744214 4 0.016682265055744213 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.655005103642106E-5 12-13 0.0 14-15 4.24125382773158E-5 16-17 0.001116863507969316 18-19 1.696501531092632E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.24125382773158E-4 28-29 0.004905716927409527 30-31 5.655005103642106E-5 32-33 1.8378766586836843E-4 34-35 0.010094184110001158 36-37 0.0036333407790900534 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.1102528070031584E-4 60-61 0.0016823640183335265 62-63 0.008680432834090632 64-65 0.002078214375588474 66-67 0.004368491442563527 68-69 0.002940602653893895 70-71 1.4137512759105264E-4 72-73 0.007125306430589053 74-75 0.006489118356429317 76-77 0.0017813266076472632 78-79 4.0998787001405267E-4 80-81 0.007408056685771159 82-83 0.009670058727228002 84-85 0.002021664324552053 86-87 0.0018520141714427898 88-89 3.393003062185264E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 2.827502551821053E-5 98-99 5.655005103642106E-5 100-101 8.199757400281053E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3536690.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.694996704723096 #Duplication Level Percentage of deduplicated Percentage of total 1 53.92404367864815 17.630464303787452 2 17.687104475313806 11.56559645072956 3 8.883386079458877 8.713268357840729 4 5.033194065890479 6.582410535940841 5 3.2893831284014685 5.377318527182887 6 2.2696718139484866 4.452414748670919 7 1.637640400637395 3.747985325165273 8 1.193721717041322 3.122298208401793 9 0.9335342100472717 2.7469708129117643 >10 4.89663603133501 27.73938810200595 >50 0.18757585856473918 4.100414587147365 >100 0.06228664496441403 3.37136563241338 >500 0.0013013541073007503 0.2908281783950559 >1k 4.337847011552577E-4 0.3182005752136958 >5k 8.675694023105154E-5 0.24107565419336785 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 8499 0.24030944187927128 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 4528 0.1280293155464573 RNA PCR Primer, Index 46 (95% over 21bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.482507655463159E-5 7 0.0 0.0 0.0 5.655005103642106E-5 8.482507655463159E-5 8 0.0 0.0 0.0 5.655005103642106E-5 8.482507655463159E-5 9 0.0 0.0 0.0 1.1310010207284212E-4 1.979251786274737E-4 10-11 0.0 0.0 0.0 1.1310010207284212E-4 1.979251786274737E-4 12-13 0.0 0.0 0.0 1.1310010207284212E-4 1.979251786274737E-4 14-15 0.0 0.0 0.0 1.4137512759105264E-4 3.393003062185264E-4 16-17 0.0 0.0 0.0 1.6965015310926317E-4 4.8067543380957904E-4 18-19 0.0 0.0 0.0 1.6965015310926317E-4 5.089504593277895E-4 20-21 0.0 0.0 0.0 1.979251786274737E-4 6.361880741597369E-4 22-23 0.0 0.0 0.0 3.958503572549474E-4 6.503255869188422E-4 24-25 0.0 0.0 0.0 8.199757400281053E-4 6.503255869188422E-4 26-27 0.0 0.0 0.0 0.0018520141714427896 7.068756379552633E-4 28-29 0.0 0.0 0.0 0.004509866570154579 7.068756379552633E-4 30-31 0.0 0.0 0.0 0.012172398485589632 7.068756379552633E-4 32-33 0.0 0.0 0.0 0.0324880043204239 7.917007145098948E-4 34-35 0.0 0.0 0.0 0.06694112291436344 9.04800816582737E-4 36-37 0.0 0.0 0.0 0.11449971583599354 9.330758421009476E-4 38-39 0.0 0.0 0.0 0.17141734220415134 0.001003763405896474 40-41 0.0 0.0 0.0 0.2257760787629111 0.001116863507969316 42-43 0.0 0.0 0.0 0.2911055252227365 0.0011592760462466318 44-45 0.0 0.0 0.0 0.3772878030022422 0.0012723761483194739 46-47 0.0 0.0 0.0 0.475571791703542 0.0013713387376332107 48-49 0.0 0.0 0.0 0.5659104982342247 0.0014420263014287371 50-51 0.0 0.0 0.0 0.6514283129140523 0.0014420263014287371 52-53 0.0 1.4137512759105265E-5 0.0 0.7481854502373688 0.0014561638141878425 54-55 0.0 1.4137512759105264E-4 0.0 0.8668415948245394 0.0014985763524651581 56-57 0.0 1.979251786274737E-4 0.0 0.9998190398366835 0.0015409888907424738 58-59 0.0 1.979251786274737E-4 0.0 1.133277160282637 0.0015551264035015791 60-61 0.0 1.979251786274737E-4 0.0 1.2634836527939965 0.0015551264035015791 62-63 0.0 1.979251786274737E-4 0.0 1.4071490574520245 0.0015551264035015791 64-65 0.0 1.979251786274737E-4 0.0 1.5758237221809093 0.0015551264035015791 66-67 0.0 1.979251786274737E-4 0.0 1.7680797581919818 0.0015551264035015791 68-69 0.0 1.979251786274737E-4 0.0 1.9743177943218093 0.0015551264035015791 70-71 0.0 1.979251786274737E-4 0.0 2.175947001292169 0.0015975389417788948 72-73 0.0 1.979251786274737E-4 0.0 2.390512032437109 0.0018802891969610003 74-75 0.0 1.979251786274737E-4 0.0 2.6357271912437903 0.0018944267097201054 76-77 0.0 1.979251786274737E-4 0.0 2.914306880161959 0.002007526811792948 78-79 0.0 1.979251786274737E-4 0.0 3.21308907481289 0.002092351888347579 80-81 0.0 1.979251786274737E-4 0.0 3.5208627275786117 0.002148901939384 82-83 0.0 1.979251786274737E-4 0.0 3.8489378486664085 0.0022620020414568422 84-85 0.0 1.979251786274737E-4 0.0 4.215551829535526 0.002290277066975053 86-87 0.0 1.979251786274737E-4 0.0 4.616435141332715 0.002360964630770579 88-89 0.0 1.979251786274737E-4 0.0 5.055079749709474 0.0024316521945661057 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3705 0.0 40.779564 1 GTATCAA 5785 0.0 37.286892 1 TCAACGC 6250 0.0 33.214783 4 CAACGCA 6500 0.0 31.859703 5 ATCAACG 6655 0.0 31.193888 3 AACGCAG 6940 0.0 29.97666 6 TATCAAC 7215 0.0 29.16779 2 ACGCAGA 7890 0.0 26.006107 7 CGCAGAG 8020 0.0 25.05155 8 CGCGGTA 50 0.001653747 23.751822 58-59 CGGAGAT 660 0.0 21.596384 1 GCAGAGT 9665 0.0 19.608286 9 GTGGTAT 2580 0.0 19.152142 1 GTACATG 7765 0.0 19.090479 1 ACATGGG 7590 0.0 17.399595 3 AGAGTAC 7770 0.0 16.046598 10-11 CAGAGTA 9355 0.0 16.018812 10-11 CATGGGG 4480 0.0 16.011387 4 TACATGG 8385 0.0 15.919865 2 GTGTACG 335 2.69476E-7 15.600968 1 >>END_MODULE