FastQCFastQC Report
Fri 10 Feb 2017
SRR2031754_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031754_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6936130
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111620.160925472850134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87630.12633846251439923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113650.042.8855171
GTATCAA179900.034.961461
TCAACGC201500.030.858544
ATCAACG204900.030.3464893
CAACGCA209250.029.7603875
TATCAAC214250.029.2453382
AACGCAG217000.028.7813366
ACGCAGA243800.025.326037
CGCAGAG249400.024.5692278
GTACATG184750.022.0101031
TACATGG186250.020.9401422
GCAGAGT282250.020.8682469
GAGTACT135250.020.81138812-13
ACATGGG193950.019.3975183
CAGAGTA265800.018.80140710-11
AGAGTAC224800.018.24717510-11
GTGGTAT66900.017.8229791
GTACTTT158350.017.71588314-15
CATGGGG124150.017.3708044
TGGTATC71300.016.1900982
Produced by FastQC (version 0.11.5)