FastQCFastQC Report
Fri 10 Feb 2017
SRR2031754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6936130
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148480.21406749873488531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126910.18296946568187158No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT72370.1043377214671582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122200.044.4447251
GTATCAA199050.035.2346081
TCAACGC228850.030.2246824
ATCAACG231650.029.8604283
CAACGCA235750.029.353655
TATCAAC243150.028.6435362
AACGCAG243750.028.4487026
ACGCAGA271600.025.3741727
CGCAGAG277350.024.5227458
GAGTACT164650.021.92425512-13
GCAGAGT316650.021.0441999
GTACATG176800.019.3237721
AGAGTAC250050.019.09027710-11
CAGAGTA299500.018.9832410-11
GTACTTT194750.018.31620414-15
TACATGG178300.018.3051092
ACATGGG178000.017.5886443
GCGGTAT6650.017.1488441
CGGTATC5900.016.9096342
AGTACTT192500.016.70440112-13
Produced by FastQC (version 0.11.5)