##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031754_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6936130 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73101181782925 34.0 31.0 34.0 31.0 34.0 2 32.892605530749854 34.0 31.0 34.0 31.0 34.0 3 32.95845190329478 34.0 31.0 34.0 31.0 34.0 4 36.35627720357029 37.0 37.0 37.0 35.0 37.0 5 36.3212558011456 37.0 37.0 37.0 35.0 37.0 6 36.34660985304485 37.0 37.0 37.0 35.0 37.0 7 36.292503168193214 37.0 37.0 37.0 35.0 37.0 8 36.28234981178265 37.0 37.0 37.0 35.0 37.0 9 38.131461924733244 39.0 39.0 39.0 37.0 39.0 10-11 38.095723191462675 39.0 39.0 39.0 36.0 39.0 12-13 38.044309723145325 39.0 39.0 39.0 35.0 39.0 14-15 39.53356396145978 41.0 39.0 41.0 37.0 41.0 16-17 39.51130428639601 41.0 39.0 41.0 37.0 41.0 18-19 39.51655548555174 41.0 39.5 41.0 36.5 41.0 20-21 39.50644509258045 41.0 39.0 41.0 37.0 41.0 22-23 39.41587643253514 41.0 39.0 41.0 36.0 41.0 24-25 39.35209490018209 41.0 39.0 41.0 36.0 41.0 26-27 39.223760656158404 41.0 39.0 41.0 36.0 41.0 28-29 39.12828955051304 40.5 39.0 41.0 36.0 41.0 30-31 38.97113512866685 40.0 38.5 41.0 35.0 41.0 32-33 38.780935190084385 40.0 38.0 41.0 35.0 41.0 34-35 38.64431902227899 40.0 38.0 41.0 35.0 41.0 36-37 38.54897010292483 40.0 38.0 41.0 34.5 41.0 38-39 38.629331918519405 40.0 38.0 41.0 35.0 41.0 40-41 38.559776200849754 40.0 38.0 41.0 35.0 41.0 42-43 38.470591814167264 40.0 38.0 41.0 35.0 41.0 44-45 38.300696497903004 40.0 38.0 41.0 34.0 41.0 46-47 38.11190447699221 40.0 38.0 41.0 34.0 41.0 48-49 37.895865201488434 40.0 38.0 41.0 33.0 41.0 50-51 37.76472932312399 40.0 38.0 41.0 33.0 41.0 52-53 37.8352388147281 40.0 38.0 41.0 33.0 41.0 54-55 37.76932403227737 40.0 37.5 41.0 33.0 41.0 56-57 37.59635034233787 40.0 37.0 41.0 33.0 41.0 58-59 37.41871893981226 40.0 37.0 41.0 33.0 41.0 60-61 37.167078399626305 39.5 36.0 41.0 33.0 41.0 62-63 36.906168713677516 39.0 36.0 41.0 32.0 41.0 64-65 36.59431541796362 39.0 35.0 41.0 32.0 41.0 66-67 36.25829598061166 38.5 35.0 40.5 31.0 41.0 68-69 35.882851965000654 37.5 35.0 40.0 31.0 41.0 70-71 35.5336950143668 37.0 35.0 39.5 31.0 41.0 72-73 35.14927683016322 36.5 35.0 39.0 30.5 41.0 74-75 34.76348626683756 36.0 35.0 39.0 30.0 40.5 76-77 33.82150442682014 35.0 33.5 37.0 29.0 39.0 78-79 34.02457126668618 35.0 34.0 37.0 30.0 39.0 80-81 33.79455726752526 35.0 34.0 37.0 30.0 39.0 82-83 33.51792945057258 35.0 34.0 36.0 29.5 38.0 84-85 33.219787330975635 35.0 34.0 36.0 29.0 37.0 86-87 32.96659124036026 35.0 34.0 36.0 29.0 37.0 88-89 32.73599860440909 35.0 34.0 35.0 29.0 36.5 90-91 32.53011794761632 35.0 34.0 35.0 29.0 36.0 92-93 32.359209458300235 35.0 34.0 35.0 29.0 36.0 94-95 32.18015882055267 35.0 34.0 35.0 28.0 36.0 96-97 32.0124006470467 35.0 34.0 35.0 27.0 35.5 98-99 31.80439654389407 35.0 33.0 35.0 27.0 35.0 100-101 31.089483112340744 34.5 32.0 35.0 24.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14217713521581743 1101 2 0.14393502588590223 1101 3 0.16802198242664446 1101 4 0.11535407856703017 1101 5 0.06985659616361772 1101 6 0.09279510911451183 1101 7 0.151714234789722 1101 8 0.12562221539330665 1101 9 0.04233357266335247 1101 10-11 0.0666756069284773 1101 12-13 0.13605671466053337 1101 14-15 0.08798592654873261 1101 16-17 0.06379259390731562 1101 18-19 0.09357223198077236 1101 20-21 0.04368457545484716 1101 22-23 -0.05193433458405394 1101 24-25 0.015960952842426934 1101 26-27 0.10150298738412289 1101 28-29 0.08198093968613307 1101 30-31 0.13201165437521212 1101 32-33 0.180283378171616 1101 34-35 -0.028998054752456426 1101 36-37 0.10138224745408309 1101 38-39 0.06996710249484295 1101 40-41 -0.17414291864486842 1101 42-43 -0.02876292732212704 1101 44-45 0.08625067885749615 1101 46-47 0.10560006041392711 1101 48-49 -0.13102055482881525 1101 50-51 -0.03233945714007547 1101 52-53 -5.241666660751321E-4 1101 54-55 -0.029516772632625532 1101 56-57 -0.1748727131203367 1101 58-59 -0.09258328253571335 1101 60-61 -0.09666158338188069 1101 62-63 0.0419608234394957 1101 64-65 0.01731721101167949 1101 66-67 -0.25119426823935953 1101 68-69 -0.24601696440562648 1101 70-71 -0.2794999350190679 1101 72-73 -0.27903865667449423 1101 74-75 -0.3220424184944619 1101 76-77 -0.3630363399547747 1101 78-79 -0.3221297715464857 1101 80-81 -0.3146423190277119 1101 82-83 -0.232944345922391 1101 84-85 -0.21417290722479976 1101 86-87 -0.12278667022576428 1101 88-89 -0.2329908676664445 1101 90-91 -0.3253499709755445 1101 92-93 -0.4303748009075896 1101 94-95 -0.3853738066490138 1101 96-97 -0.37310157082163187 1101 98-99 -0.5894467537585086 1101 100-101 -0.7465133108499451 1102 1 0.1286028598854898 1102 2 0.08743799211238468 1102 3 0.10554591201591279 1102 4 0.042301621417280444 1102 5 0.09589408924742315 1102 6 0.1081865617765203 1102 7 0.14392301932290508 1102 8 0.06811480393089653 1102 9 0.054271563198668105 1102 10-11 0.18600358997166921 1102 12-13 0.16524576330332508 1102 14-15 0.21506209061778492 1102 16-17 0.19127274933648408 1102 18-19 0.16696982238925528 1102 20-21 0.2053967948906319 1102 22-23 0.1726575473339338 1102 24-25 0.11554455691562993 1102 26-27 0.1932673814211867 1102 28-29 0.19636654288806454 1102 30-31 0.04102946675919128 1102 32-33 0.20082901110900764 1102 34-35 0.11383087382098722 1102 36-37 0.1575966883986979 1102 38-39 -0.002825210719940685 1102 40-41 0.05864702427430757 1102 42-43 0.12678220532141182 1102 44-45 0.07235284664277941 1102 46-47 0.08785818785259636 1102 48-49 0.12163699936153449 1102 50-51 0.02181601502626762 1102 52-53 0.1604920039613731 1102 54-55 0.25041361450077204 1102 56-57 0.2520550723370931 1102 58-59 0.21924519348104354 1102 60-61 0.26317317205487 1102 62-63 0.20856199198280478 1102 64-65 0.1793862909610624 1102 66-67 0.1574300230196286 1102 68-69 0.21448074620647617 1102 70-71 0.19130925651048614 1102 72-73 0.12672717166647374 1102 74-75 0.12458403477933899 1102 76-77 0.18472885786508897 1102 78-79 0.21801458874270452 1102 80-81 -0.001214337135067467 1102 82-83 0.1045041151586048 1102 84-85 0.1402929393858372 1102 86-87 0.13091165195454835 1102 88-89 0.04556089414433728 1102 90-91 -0.06872126955887836 1102 92-93 0.006985819633072765 1102 94-95 0.042367743819490045 1102 96-97 -0.05969000412811454 1102 98-99 -0.09894988224405665 1102 100-101 -0.07991180217188187 1103 1 0.25429543950006206 1103 2 0.16561303347236134 1103 3 0.19656904165873357 1103 4 0.14351234268988122 1103 5 0.12444464068213534 1103 6 0.13324095609573305 1103 7 0.13824885020230937 1103 8 0.1538525129519499 1103 9 0.1828985066187414 1103 10-11 0.16810571112690553 1103 12-13 0.12388635274525228 1103 14-15 0.2248917445120142 1103 16-17 0.24060812262390385 1103 18-19 0.27527125675642594 1103 20-21 0.27037925982482136 1103 22-23 0.22712712190361373 1103 24-25 0.12566662961321384 1103 26-27 0.1978446363488473 1103 28-29 0.19528268146098782 1103 30-31 0.2372596465735839 1103 32-33 0.18555065655456104 1103 34-35 0.16885414926930054 1103 36-37 0.188966835671593 1103 38-39 0.10762553657429663 1103 40-41 0.08592183437215084 1103 42-43 0.19665582932108094 1103 44-45 0.21282679781477754 1103 46-47 0.21105889384141108 1103 48-49 0.2433395000985641 1103 50-51 0.10119818921115353 1103 52-53 0.15861144195604737 1103 54-55 0.25520229305605113 1103 56-57 0.17249662203742133 1103 58-59 0.25971204145407967 1103 60-61 0.1996303520809164 1103 62-63 0.11666200275665517 1103 64-65 0.2306957498172011 1103 66-67 0.23528656838929862 1103 68-69 0.1968343704918638 1103 70-71 0.2384121958374621 1103 72-73 0.29676684215400684 1103 74-75 0.37630112541901894 1103 76-77 0.37410890080684567 1103 78-79 0.419650470203635 1103 80-81 0.3288881555633836 1103 82-83 0.38238653079840645 1103 84-85 0.29040989415204166 1103 86-87 0.3179251760088704 1103 88-89 0.319812118191912 1103 90-91 0.30151825138732136 1103 92-93 0.36952413786256955 1103 94-95 0.28955946463009496 1103 96-97 0.14190068159957292 1103 98-99 0.38841732216160096 1103 100-101 0.3413152377507913 1104 1 0.15693451021984117 1104 2 0.09193686953849323 1104 3 0.14064851590763539 1104 4 0.07665056156801597 1104 5 0.12246744757525363 1104 6 0.06596123040102952 1104 7 0.1037990344859594 1104 8 0.12912385625760692 1104 9 0.07904933141659143 1104 10-11 0.15042582034543983 1104 12-13 0.15117807945833306 1104 14-15 0.225002158947035 1104 16-17 0.18491019075700166 1104 18-19 0.19220304830992063 1104 20-21 0.24471591361236023 1104 22-23 0.1693716146821984 1104 24-25 0.19213032593980728 1104 26-27 0.23533889937917962 1104 28-29 0.21593144730920244 1104 30-31 0.24153775794053445 1104 32-33 0.21862275072687254 1104 34-35 0.0884001962519747 1104 36-37 0.1361915516686878 1104 38-39 0.17966789023117968 1104 40-41 0.14179552661793338 1104 42-43 0.22910740649461303 1104 44-45 0.17753726650983737 1104 46-47 0.15550349697245736 1104 48-49 0.2686440768691938 1104 50-51 0.34768617482718156 1104 52-53 0.3862822478997714 1104 54-55 0.3309993438063188 1104 56-57 0.26283682353035687 1104 58-59 0.33249776248419494 1104 60-61 0.5026134766054398 1104 62-63 0.43479427070768395 1104 64-65 0.44038566943603286 1104 66-67 0.37416560224156825 1104 68-69 0.3624853141789117 1104 70-71 0.4376161528967941 1104 72-73 0.3699387105554166 1104 74-75 0.42012666937213794 1104 76-77 0.4193139313181433 1104 78-79 0.5411537076976884 1104 80-81 0.45615349059918486 1104 82-83 0.3950007096987349 1104 84-85 0.4530564603884173 1104 86-87 0.37976682572960385 1104 88-89 0.3823904524841879 1104 90-91 0.37715007795008404 1104 92-93 0.3443857418901928 1104 94-95 0.36982469586688893 1104 96-97 0.4212065454562506 1104 98-99 0.45970575878051534 1104 100-101 0.4737684478992499 1105 1 0.14827572206557704 1105 2 0.12894256863992837 1105 3 0.09357719795700348 1105 4 0.10071497108064165 1105 5 0.09471265907546922 1105 6 0.07397200542534677 1105 7 0.1139029078593694 1105 8 0.14883938450939382 1105 9 0.06387555098522313 1105 10-11 0.12757552922028736 1105 12-13 0.18839947443218108 1105 14-15 0.1785625291109767 1105 16-17 0.2203682526923174 1105 18-19 0.16107880234567062 1105 20-21 0.13265964552630294 1105 22-23 0.2018365171845815 1105 24-25 0.20393061264307022 1105 26-27 0.1884853854131947 1105 28-29 0.2958508300128102 1105 30-31 0.26413962603646013 1105 32-33 0.13783518570961206 1105 34-35 0.17761350018828637 1105 36-37 0.3222682674149482 1105 38-39 0.21662921614122155 1105 40-41 0.1734752601525429 1105 42-43 0.13141625213349784 1105 44-45 0.25301627074045996 1105 46-47 0.2216062350503094 1105 48-49 0.24337575531729527 1105 50-51 0.32907912797362115 1105 52-53 0.3198820860378859 1105 54-55 0.24080018890223442 1105 56-57 0.20879575236254055 1105 58-59 0.20285485098582967 1105 60-61 0.24564768252584201 1105 62-63 0.17738363924065936 1105 64-65 0.21712211118940417 1105 66-67 0.25023768014094117 1105 68-69 0.21677789341822518 1105 70-71 0.1746854295777851 1105 72-73 0.142125948969543 1105 74-75 0.19642367256206938 1105 76-77 0.14257766952557205 1105 78-79 0.25938191135983857 1105 80-81 0.2341480897185093 1105 82-83 0.3242847279457308 1105 84-85 0.3984272516194878 1105 86-87 0.3121813134994653 1105 88-89 0.2633854865944727 1105 90-91 0.30193124561801454 1105 92-93 0.24606228240604366 1105 94-95 0.2502808759264141 1105 96-97 0.3450983468535682 1105 98-99 0.3341182638965421 1105 100-101 0.25159280410234786 1106 1 0.09855434468695279 1106 2 0.06546638760482182 1106 3 0.0363072654036074 1106 4 0.08114362088561933 1106 5 0.06780905627417866 1106 6 0.08048319164530682 1106 7 0.0854588347408125 1106 8 0.054400720319812024 1106 9 0.06680917163016886 1106 10-11 0.06786956870362815 1106 12-13 0.04812942996819203 1106 14-15 0.08790420950177946 1106 16-17 0.09118819997275551 1106 18-19 0.15346145069605654 1106 20-21 0.1080273189498655 1106 22-23 0.1325494843957742 1106 24-25 0.1290020083715575 1106 26-27 0.09574361822343747 1106 28-29 0.0742431413832918 1106 30-31 0.1270609067402333 1106 32-33 0.17167558235447444 1106 34-35 0.15603086116791332 1106 36-37 0.16598683288499672 1106 38-39 0.15262500820390557 1106 40-41 0.19157924171285146 1106 42-43 0.16574002310836278 1106 44-45 0.21452015795841106 1106 46-47 0.17709445677123625 1106 48-49 0.1866949166181655 1106 50-51 0.18510007882650825 1106 52-53 0.2717693551044391 1106 54-55 0.1431194179449946 1106 56-57 0.23916167268568245 1106 58-59 0.28372044835634824 1106 60-61 0.3368819942620789 1106 62-63 0.3232230964321303 1106 64-65 0.3973989372860629 1106 66-67 0.32408258794870193 1106 68-69 0.3833904741036491 1106 70-71 0.2341350875557069 1106 72-73 0.26904136317642013 1106 74-75 0.2701300817136243 1106 76-77 0.19371361160799694 1106 78-79 0.1776040441969613 1106 80-81 0.15134931760841397 1106 82-83 0.15300538894772586 1106 84-85 0.23525922171976532 1106 86-87 0.2063102136516477 1106 88-89 0.24423790779614052 1106 90-91 0.3065180163970922 1106 92-93 0.3042897576709578 1106 94-95 0.3836170545843416 1106 96-97 0.34973888930478125 1106 98-99 0.34732894885137 1106 100-101 0.4318845557462012 1107 1 0.2175685728498209 1107 2 0.1582073124005703 1107 3 0.1674077089365298 1107 4 0.13855078874277638 1107 5 0.0935476556424959 1107 6 0.11149738056001723 1107 7 0.14912916355141448 1107 8 0.11893724873178968 1107 9 0.15408875660801158 1107 10-11 0.20585425445751326 1107 12-13 0.18808286505255722 1107 14-15 0.24993310402906133 1107 16-17 0.2506784639628208 1107 18-19 0.24091645198819123 1107 20-21 0.21564632379657667 1107 22-23 0.22588475525910212 1107 24-25 0.23490030495701575 1107 26-27 0.23741914464918068 1107 28-29 0.25358265381414924 1107 30-31 0.24536489715866594 1107 32-33 0.2896664616977773 1107 34-35 0.24217836896059453 1107 36-37 0.24372424791918945 1107 38-39 0.1850129479852214 1107 40-41 0.27502890817786607 1107 42-43 0.2798307730501577 1107 44-45 0.31286773732157513 1107 46-47 0.32675869319214 1107 48-49 0.2654000963080634 1107 50-51 0.29180210154466835 1107 52-53 0.34765422563521753 1107 54-55 0.34277735613211746 1107 56-57 0.3463976948146481 1107 58-59 0.29544117827715155 1107 60-61 0.35469840196091695 1107 62-63 0.3938918107184861 1107 64-65 0.3420799333761977 1107 66-67 0.3739121990002161 1107 68-69 0.3302155705989307 1107 70-71 0.39224583349597 1107 72-73 0.3143330361180574 1107 74-75 0.33632220923716005 1107 76-77 0.34471485593648765 1107 78-79 0.40343608237143513 1107 80-81 0.4512764684291497 1107 82-83 0.4538760091863523 1107 84-85 0.4723579872372312 1107 86-87 0.47354084124617657 1107 88-89 0.48710628342619344 1107 90-91 0.39317357127618635 1107 92-93 0.4607333831811786 1107 94-95 0.44284431675347236 1107 96-97 0.49650990475725365 1107 98-99 0.5337482527669692 1107 100-101 0.6212770905111604 1108 1 0.014030447049115935 1108 2 -0.015655863299713246 1108 3 -0.024432210785349184 1108 4 0.03811544662629984 1108 5 0.029454793052750006 1108 6 0.01302981009806814 1108 7 -0.004494060851897075 1108 8 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-0.49233599482530366 2316 50-51 -0.3797966202487899 2316 52-53 -0.49502105801321505 2316 54-55 -0.48050496784858865 2316 56-57 -0.4647862827484559 2316 58-59 -0.4729870888435386 2316 60-61 -0.5025942223786117 2316 62-63 -0.47252105890157736 2316 64-65 -0.43636732908158393 2316 66-67 -0.41286263391357636 2316 68-69 -0.5501964731800797 2316 70-71 -0.45909100514162304 2316 72-73 -0.6083542685627705 2316 74-75 -0.48575208199805786 2316 76-77 -0.9056613530623494 2316 78-79 -0.6350522005697812 2316 80-81 -0.4510547882829812 2316 82-83 -0.4228672567736069 2316 84-85 -0.36061814874270937 2316 86-87 -0.2847580034555648 2316 88-89 -0.423047011237891 2316 90-91 -0.434595492166018 2316 92-93 -0.3561553820478096 2316 94-95 -0.3003603805073922 2316 96-97 -0.2804188014127931 2316 98-99 -0.3133615126919054 2316 100-101 -0.10924544771031108 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 11.0 4 29.0 5 59.0 6 135.0 7 301.0 8 880.0 9 1871.0 10 3509.0 11 5575.0 12 8411.0 13 11273.0 14 14362.0 15 17214.0 16 21227.0 17 29850.0 18 29327.0 19 18729.0 20 15463.0 21 15789.0 22 17127.0 23 18395.0 24 21114.0 25 25042.0 26 29098.0 27 36458.0 28 47235.0 29 61518.0 30 79338.0 31 102750.0 32 133501.0 33 178520.0 34 253977.0 35 395332.0 36 708853.0 37 1515433.0 38 2354080.0 39 763911.0 40 428.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.518464202850275 20.339780555086904 14.57220643222486 23.56954880983796 2 15.74419741267825 21.74431563422254 39.40256310074927 23.108923852349943 3 18.282832433320863 25.863064021632017 31.210486136491923 24.6436174085552 4 13.24169742442359 18.692511257656275 37.91936824461314 30.146423073306998 5 13.615618507726932 37.22929068515152 35.25983509536297 13.895255711758574 6 29.708756900461786 37.221130515143166 18.817914312448007 14.252198271947037 7 25.58377654398058 32.91776538213673 23.520017646728075 17.978440427154624 8 24.089211130702566 34.556013223512245 22.126632574648973 19.228143071136213 9 25.168746837213256 16.47492189448583 21.875109030540084 36.481222237760825 10-11 23.48470043483191 26.452270888579992 29.782262540262916 20.280766136325184 12-13 24.379605369248083 24.748961218212546 29.144186249694442 21.727247162844925 14-15 21.967241500241453 26.548438764102166 27.012249709798276 24.472070025858102 16-17 21.409643372772756 29.00142703947124 27.858526726641347 21.73040286111466 18-19 21.239410506161946 28.11763637307313 29.565239644066132 21.07771347669879 20-21 22.182196700465536 27.27464738982689 29.307726354609848 21.235429555097728 22-23 21.97636866667724 27.247269010240583 28.981665568551918 21.794696754530264 24-25 21.819191681816804 27.43391487760466 29.1469594716362 21.599933968942338 26-27 21.604026465852787 27.562289079285463 29.313775774092317 21.519908680769436 28-29 21.595223122766416 27.4778936853859 29.329043190676934 21.597840001170745 30-31 21.995822162348887 27.30414710002301 29.043129851773124 21.65690088585498 32-33 21.937019544067866 27.421173509108836 28.843157269482074 21.798649677341224 34-35 22.017862018255656 27.417337933642816 28.90149214950895 21.663307898592578 36-37 21.966833923938648 27.32936341215216 28.965932228364572 21.737870435544625 38-39 21.817627408944183 27.42364978741748 29.020909354351776 21.737813449286563 40-41 21.947743193971277 27.290145945938153 28.973671197050805 21.788439663039764 42-43 22.089255824213215 27.28951879506295 28.697926653623853 21.923298727099983 44-45 22.046948370344847 27.429243684879033 28.600689433444877 21.923118511331246 46-47 22.160390592448525 27.369837358873035 28.347630450986355 22.122141597692085 48-49 22.02941698036225 27.395168487326504 28.487362549433186 22.08805198287806 50-51 22.00227648559067 27.56262497963562 28.354067758245595 22.08103077652812 52-53 22.135383852378776 27.59996568691763 28.198938024517993 22.065712436185596 54-55 22.113022679794064 27.549699904701903 28.12794022026692 22.209337195237115 56-57 22.117989426380415 27.66265914854537 28.075735316379596 22.14361610869462 58-59 21.981815468745832 27.698762203337168 28.241320970024287 22.07810135789271 60-61 22.160685068364607 27.75197002714169 28.189628083644934 21.897716820848764 62-63 22.021675875209258 27.80131810280112 28.081975650344383 22.095030371645237 64-65 22.069214792327653 27.76233734440181 28.003161488206267 22.165286375064266 66-67 22.04148531900344 27.76894038080378 27.93739960607301 22.252174694119773 68-69 21.99616777310564 27.739735853164287 28.001835374280105 22.262260999449964 70-71 22.00905635742855 27.515757364129406 28.13061102443123 22.344575254010817 72-73 22.081052152968315 27.532174186238322 28.000630943435617 22.386142717357743 74-75 22.03225121589975 27.69187570265903 27.87169874162081 22.404174339820415 76-77 22.077754872375934 27.63912508557687 27.844581662194962 22.438538379852236 78-79 22.067135859282295 27.64163778215696 27.75011373467177 22.541112623888974 80-81 22.06663317768261 27.683244771119035 27.748891446380952 22.501230604817398 82-83 22.11142502195966 27.638021444625604 27.78923743022076 22.46131610319397 84-85 22.162286815622668 27.54414639419301 27.68905730843872 22.6045094817456 86-87 22.060176549693754 27.568648101952025 27.76341432733457 22.607761021019655 88-89 22.084111418560653 27.53420781642703 27.681790805276307 22.699889959736005 90-91 22.116938560619435 27.52591340747026 27.66307924325807 22.69406878865223 92-93 22.185584756917763 27.46039217834729 27.757070585470572 22.596952479264374 94-95 22.140559649256865 27.374717601890392 27.75580907508942 22.728913673763323 96-97 22.119640966807488 27.33608919386214 27.701624573654392 22.842645265675984 98-99 22.07025302877917 27.356660661394944 27.652719211640846 22.92036709818504 100-101 21.99514493994251 27.475220991737025 27.60732764446687 22.92230642385359 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4619.0 1 2787.5 2 1062.5 3 1126.0 4 878.5 5 816.0 6 1288.5 7 2499.0 8 3934.5 9 4381.5 10 3343.0 11 2427.5 12 2430.0 13 2538.0 14 2826.0 15 3101.0 16 3453.5 17 3872.5 18 4174.5 19 4564.0 20 5157.0 21 5826.0 22 6619.0 23 7988.0 24 9956.0 25 13080.0 26 18662.5 27 27477.5 28 40011.5 29 58716.0 30 83032.5 31 109437.5 32 138452.5 33 167966.0 34 192453.5 35 210814.5 36 229122.0 37 242250.0 38 246391.5 39 245929.0 40 246468.5 41 249199.5 42 249985.0 43 252331.5 44 256240.0 45 259410.0 46 263727.0 47 267712.5 48 270113.0 49 264334.5 50 249233.5 51 234677.0 52 223592.0 53 214358.0 54 199351.5 55 180701.5 56 160792.5 57 141087.0 58 128025.5 59 114402.0 60 95831.5 61 78535.5 62 62570.5 63 45503.0 64 31781.5 65 22923.0 66 16177.0 67 11961.0 68 9250.5 69 6690.0 70 4725.5 71 3464.0 72 2487.5 73 1705.5 74 1202.0 75 878.5 76 563.0 77 347.5 78 222.5 79 131.0 80 89.0 81 62.5 82 43.0 83 25.0 84 15.0 85 15.5 86 12.0 87 9.5 88 5.5 89 1.5 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019607475638432383 2 0.0 3 0.001657985072367444 4 0.018655936379508457 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.162589224827101E-5 12-13 7.20863074942367E-6 14-15 9.37121997425077E-5 16-17 0.0013263880578939552 18-19 1.441726149884734E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.2438838372406516E-4 28-29 0.005557854307805649 30-31 8.650356899308404E-5 32-33 1.441726149884734E-4 34-35 0.011526600568328448 36-37 0.00444051654164498 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.162589224827101E-4 60-61 0.001751697272109952 62-63 0.009104500636522095 64-65 0.002249092793820185 66-67 0.00471444451012308 68-69 0.0030997112222521783 70-71 1.1533809199077872E-4 72-73 0.006985163196191537 74-75 0.006091292983263001 76-77 0.0016507764416180206 78-79 4.3972647571484386E-4 80-81 0.007439306933405227 82-83 0.009890241388209275 84-85 0.0020832942865834404 86-87 0.0017661145336087991 88-89 4.685609987125386E-4 90-91 7.20863074942367E-6 92-93 0.0 94-95 0.0 96-97 3.604315374711835E-5 98-99 2.883452299769468E-5 100-101 9.731651511721954E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6936130.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.427593682574617 #Duplication Level Percentage of deduplicated Percentage of total 1 49.947978534402274 15.697447746451534 2 18.297922788298237 11.501193652515196 3 9.831664468276744 9.269566683972224 4 5.880989134380222 7.393013478677513 5 3.7713847025156197 5.926277302566922 6 2.617648332123499 4.935983291150787 7 1.9259793906899587 4.237022841213165 8 1.3518142885157167 3.3987416155016508 9 1.042853420509111 2.9496936213219116 >10 5.101373519363835 26.65247849531894 >50 0.16956880879616523 3.581382888737518 >100 0.058344529621879 3.1123939555081837 >500 0.0016062043232567956 0.34283518055042367 >1k 6.883248847535373E-4 0.4083042373129598 >5k 9.177664943240413E-5 0.19650867036008715 >10k+ 9.177664943240413E-5 0.39715633884092466 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14848 0.21406749873488531 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12691 0.18296946568187158 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 7237 0.1043377214671582 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.441726149884734E-5 2 0.0 0.0 0.0 0.0 1.441726149884734E-5 3 0.0 0.0 0.0 0.0 1.441726149884734E-5 4 0.0 0.0 0.0 0.0 1.441726149884734E-5 5 0.0 0.0 0.0 0.0 1.441726149884734E-5 6 0.0 0.0 0.0 0.0 2.883452299769468E-5 7 0.0 0.0 0.0 1.441726149884734E-5 2.883452299769468E-5 8 0.0 0.0 0.0 1.441726149884734E-5 2.883452299769468E-5 9 0.0 0.0 0.0 4.325178449654202E-5 2.883452299769468E-5 10-11 0.0 0.0 0.0 4.325178449654202E-5 2.883452299769468E-5 12-13 0.0 0.0 0.0 5.046041524596569E-5 1.0092083049193139E-4 14-15 0.0 0.0 0.0 7.929493824366037E-5 2.0184166098386277E-4 16-17 0.0 0.0 0.0 1.2975535348962606E-4 2.8113659922752315E-4 18-19 0.0 0.0 0.0 1.3696398423904973E-4 3.892660604688782E-4 20-21 0.0 0.0 0.0 1.8021576873559174E-4 3.9647469121830185E-4 22-23 0.0 0.0 0.0 2.6671933772867576E-4 4.325178449654202E-4 24-25 0.0 0.0 0.0 3.820574297194545E-4 4.325178449654202E-4 26-27 0.0 0.0 0.0 7.280717056917907E-4 4.613523679631149E-4 28-29 0.0 0.0 0.0 0.0016651937031168678 4.613523679631149E-4 30-31 0.0 0.0 0.0 0.004166588573166882 4.613523679631149E-4 32-33 0.0 0.0 0.0 0.010935492846875706 4.757696294619622E-4 34-35 0.0 0.0 0.0 0.02160426635602274 5.190214139585042E-4 36-37 0.0 0.0 0.0 0.03757859209674559 5.334386754573516E-4 38-39 0.0 0.0 0.0 0.056544499598479264 5.838990907033173E-4 40-41 0.0 0.0 0.0 0.07623847880590473 5.91107721452741E-4 42-43 0.0 0.0 0.0 0.09756160856269995 5.91107721452741E-4 44-45 0.0 0.0 0.0 0.12517787296374203 6.055249829515883E-4 46-47 0.0 0.0 0.0 0.15742928693666353 6.631940289469776E-4 48-49 0.0 0.0 0.0 0.18841919052843586 7.20863074942367E-4 50-51 0.0 0.0 0.0 0.21911354025948188 8.073666439354511E-4 52-53 0.0 0.0 0.0 0.25357079524172704 9.515392589239245E-4 54-55 0.0 0.0 0.0 0.2942851417144719 0.0010092083049193137 56-57 0.0 0.0 0.0 0.3407231410022592 0.0010452514586664322 58-59 0.0 0.0 0.0 0.3869448813675638 0.0010524600894158559 60-61 0.0 0.0 0.0 0.43388027617706126 0.0010596687201652796 62-63 0.0 0.0 0.0 0.48585450388040596 0.0010957118739123978 64-65 0.0 0.0 0.0 0.5493120803675825 0.0011245463969100926 66-67 0.0 0.0 0.0 0.6281240403510315 0.0011389636584089398 68-69 0.0 0.0 0.0 0.7125515236882816 0.0011461722891583635 70-71 0.0 0.0 0.0 0.7958688778901203 0.0011822154429054817 72-73 0.0 0.0 0.0 0.8851549783524819 0.001225467227402024 74-75 0.0 0.0 0.0 0.9865804129968729 0.0012398844889008713 76-77 0.0 0.0 0.0 1.1003686493765255 0.0012398844889008713 78-79 0.0 0.0 0.0 1.2242345515438724 0.0012759276426479896 80-81 0.0 0.0 0.0 1.35784652248444 0.0013047621656456843 82-83 0.0 0.0 0.0 1.5005053250155345 0.001398474365388192 84-85 0.0 0.0 0.0 1.6595277193478206 0.001398474365388192 86-87 0.0 0.0 0.0 1.850758275868532 0.0015354383496272417 88-89 0.0 0.0 0.0 2.0784068349353313 0.0016435678108685967 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12220 0.0 44.444725 1 GTATCAA 19905 0.0 35.234608 1 TCAACGC 22885 0.0 30.224682 4 ATCAACG 23165 0.0 29.860428 3 CAACGCA 23575 0.0 29.35365 5 TATCAAC 24315 0.0 28.643536 2 AACGCAG 24375 0.0 28.448702 6 ACGCAGA 27160 0.0 25.374172 7 CGCAGAG 27735 0.0 24.522745 8 GAGTACT 16465 0.0 21.924255 12-13 GCAGAGT 31665 0.0 21.044199 9 GTACATG 17680 0.0 19.323772 1 AGAGTAC 25005 0.0 19.090277 10-11 CAGAGTA 29950 0.0 18.98324 10-11 GTACTTT 19475 0.0 18.316204 14-15 TACATGG 17830 0.0 18.305109 2 ACATGGG 17800 0.0 17.588644 3 GCGGTAT 665 0.0 17.148844 1 CGGTATC 590 0.0 16.909634 2 AGTACTT 19250 0.0 16.704401 12-13 >>END_MODULE