FastQCFastQC Report
Fri 10 Feb 2017
SRR2031751_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031751_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341199
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560.1336463471463867No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3880.11371662871227642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.10170018083288637No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG250.005017634557.0017622
GGTATCA4800.040.5741731
GTATCAA7500.039.9012341
TCAACGC8400.034.4951134
CAACGCA8700.033.305635
ATCAACG8800.032.9271553
AACGCAG9050.032.0128636
TATCAAC9100.031.8416422
ACCGTCC600.00394364631.6722988
GTATATG902.407504E-531.6676451
ATACTGC1351.19325705E-831.6629946
ACGCAGA10700.027.5201747
CGGATAC700.00838215527.1436983
CGCAGAG10550.026.5687078
TAACCGT908.93295E-426.3897045
TTAACCG950.001224560725.0007724
GTGTTAT1000.001650966723.7507341
GTGGTAT2903.6379788E-1222.9317441
TACTCTC1252.2509047E-422.8007055
GCAGAGT12200.022.1965689
Produced by FastQC (version 0.11.5)