##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031751_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 341199 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.884489696628652 33.0 31.0 34.0 28.0 34.0 2 31.23067769835199 33.0 31.0 34.0 28.0 34.0 3 31.32549040296132 34.0 31.0 34.0 28.0 34.0 4 34.70425470180159 37.0 35.0 37.0 33.0 37.0 5 34.680306800430245 37.0 35.0 37.0 32.0 37.0 6 34.69079921101762 37.0 35.0 37.0 32.0 37.0 7 34.652780342263604 37.0 35.0 37.0 32.0 37.0 8 34.63028613800158 37.0 35.0 37.0 32.0 37.0 9 36.31123479259904 39.0 37.0 39.0 32.0 39.0 10-11 36.22145287647385 39.0 37.0 39.0 32.0 39.0 12-13 36.153697695479764 39.0 37.0 39.0 32.0 39.0 14-15 37.45714524368477 41.0 38.0 41.0 32.0 41.0 16-17 37.36553887907057 40.0 38.0 41.0 32.0 41.0 18-19 37.350658999586756 40.0 38.0 41.0 32.0 41.0 20-21 37.330405423228086 40.0 38.0 41.0 32.0 41.0 22-23 37.21853962057333 40.0 38.0 41.0 32.0 41.0 24-25 37.13439371158766 40.0 38.0 41.0 31.5 41.0 26-27 37.00469374177533 40.0 38.0 41.0 31.0 41.0 28-29 36.89196627188239 40.0 38.0 41.0 31.0 41.0 30-31 36.73827297266405 40.0 38.0 41.0 30.0 41.0 32-33 36.55724225452009 40.0 37.0 41.0 30.0 41.0 34-35 36.40119988628337 40.0 37.0 41.0 30.0 41.0 36-37 36.240484292157944 40.0 37.0 41.0 29.5 41.0 38-39 36.06244449719958 40.0 36.5 41.0 28.5 41.0 40-41 35.97947825169476 40.0 36.5 41.0 28.0 41.0 42-43 35.95893305666195 40.0 36.5 41.0 27.0 41.0 44-45 35.83578937804624 40.0 36.0 41.0 27.0 41.0 46-47 35.79724588876286 40.0 36.5 41.0 26.5 41.0 48-49 35.87628920366121 40.0 37.0 41.0 27.0 41.0 50-51 35.36181378022796 39.5 35.5 40.5 26.5 41.0 52-53 35.379475027769715 39.5 35.5 40.5 26.0 41.0 54-55 35.60666649081621 40.0 36.0 41.0 26.0 41.0 56-57 35.47359898475669 40.0 35.0 41.0 26.0 41.0 58-59 35.27858668987893 39.0 35.0 41.0 26.0 41.0 60-61 35.04556285334951 39.0 35.0 41.0 25.0 41.0 62-63 34.76497879536575 39.0 35.0 41.0 23.5 41.0 64-65 34.49539711429401 38.5 35.0 41.0 23.0 41.0 66-67 34.21569377401458 37.5 35.0 40.5 22.5 41.0 68-69 33.890103722461085 37.0 34.0 40.0 22.0 41.0 70-71 33.54055990785436 36.5 34.0 39.5 21.5 41.0 72-73 33.18325669184259 36.0 34.0 39.0 20.0 41.0 74-75 32.78239824852945 35.5 34.0 39.0 19.5 40.5 76-77 32.41409265560567 35.0 34.0 37.5 18.0 39.5 78-79 32.03153877942198 35.0 33.0 37.0 16.0 39.0 80-81 31.673845761564365 35.0 33.0 37.0 12.5 39.0 82-83 31.3482923455227 35.0 33.0 36.0 9.0 38.0 84-85 31.019544312849685 35.0 33.0 36.0 7.0 37.0 86-87 30.7328611748569 35.0 32.0 35.5 2.0 37.0 88-89 30.462420757387918 35.0 32.0 35.0 2.0 36.5 90-91 30.209511165038585 35.0 32.0 35.0 2.0 36.0 92-93 29.986504942863256 35.0 32.0 35.0 2.0 36.0 94-95 29.72158329889595 35.0 31.5 35.0 2.0 36.0 96-97 29.439322799890974 35.0 31.0 35.0 2.0 35.0 98-99 29.183356340434763 35.0 31.0 35.0 2.0 35.0 100-101 28.57600696367809 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.005473952218043365 1101 2 0.04002186649827877 1101 3 0.17346003297938495 1101 4 0.13082499190035435 1101 5 -0.2933602720666002 1101 6 -0.12309380048080953 1101 7 0.13421213546916277 1101 8 -0.15983203463380846 1101 9 0.07414963582834844 1101 10-11 0.26945863306907114 1101 12-13 0.3543225740520768 1101 14-15 0.026702758405072302 1101 16-17 0.32964921864635244 1101 18-19 0.4641342927768619 1101 20-21 0.3680404891447182 1101 22-23 0.44766348201061845 1101 24-25 0.6311655695412384 1101 26-27 0.49748095982727847 1101 28-29 0.4200815993143081 1101 30-31 0.41975166728013846 1101 32-33 0.4016956105031184 1101 34-35 0.39850128731531953 1101 36-37 0.3095779714133826 1101 38-39 0.18515212854501328 1101 40-41 0.21009504507934196 1101 42-43 0.25820580538054827 1101 44-45 -0.19381779271230215 1101 46-47 0.13118484011845766 1101 48-49 0.6859079666063721 1101 50-51 0.3300599184987263 1101 52-53 0.42390942389781117 1101 54-55 0.45830730566606803 1101 56-57 0.34728346413255196 1101 58-59 0.23701261035062515 1101 60-61 0.07607849541476241 1101 62-63 0.5003367598880217 1101 64-65 0.6139170394109286 1101 66-67 0.45581057408458747 1101 68-69 -0.054102030467703344 1101 70-71 -0.27682686237101706 1101 72-73 0.31382657911074574 1101 74-75 0.1565060919673371 1101 76-77 0.16707411915322012 1101 78-79 0.16178640180967108 1101 80-81 -0.044489930377526576 1101 82-83 -0.014058075974009654 1101 84-85 0.014415418861460694 1101 86-87 -0.006158970671382491 1101 88-89 0.17350412709716778 1101 90-91 -0.4290735834174022 1101 92-93 -0.19276158816353117 1101 94-95 -0.5587309234608817 1101 96-97 -0.6008901108910258 1101 98-99 -0.6316113274206003 1101 100-101 -0.811501262627079 1102 1 -0.008729192065082714 1102 2 0.10578013166116307 1102 3 0.17457334920959866 1102 4 0.008033472465726277 1102 5 0.13578683089094312 1102 6 0.4099789523145887 1102 7 0.29012481275267277 1102 8 -0.21661116237473976 1102 9 0.05047956467291925 1102 10-11 0.1343177743634172 1102 12-13 0.309826228633618 1102 14-15 0.021862253056319503 1102 16-17 0.1043418465567072 1102 18-19 0.00765648649388595 1102 20-21 0.25634582822211627 1102 22-23 0.2523914456100229 1102 24-25 0.0887203882643206 1102 26-27 0.008051413382638373 1102 28-29 0.17144904207533074 1102 30-31 -0.01497411809185678 1102 32-33 -0.18049617031879706 1102 34-35 0.14853759110543052 1102 36-37 0.04685349297783148 1102 38-39 0.08087554206938563 1102 40-41 -0.14387900981199664 1102 42-43 0.2084817141854316 1102 44-45 -0.10752973423898737 1102 46-47 -0.24533386843472016 1102 48-49 -0.3148302104593128 1102 50-51 -0.16652752523039993 1102 52-53 -0.1429440056002278 1102 54-55 -0.37451374085118516 1102 56-57 -0.3414720419422892 1102 58-59 -0.3869829847895119 1102 60-61 -0.5787450681652757 1102 62-63 -0.3095826456979296 1102 64-65 -0.48733423122172326 1102 66-67 -0.485056602448644 1102 68-69 -0.3695154096244835 1102 70-71 -0.5423346313820971 1102 72-73 -0.7102004793141603 1102 74-75 -1.1257800787088819 1102 76-77 -0.575102414076845 1102 78-79 -0.7438297945212184 1102 80-81 -0.6398176325896365 1102 82-83 -0.39652455547446763 1102 84-85 -0.4333192246353228 1102 86-87 -0.3666011508349918 1102 88-89 -0.3727348002468496 1102 90-91 -0.481272382972076 1102 92-93 -0.38061555011715953 1102 94-95 -0.4106901180007938 1102 96-97 -0.5765544702501444 1102 98-99 -0.5363743846837785 1102 100-101 -0.2505229964960982 1103 1 0.27074294215471895 1103 2 0.38704825892009964 1103 3 0.3348071629617735 1103 4 0.21894552458299188 1103 5 0.49870013239467426 1103 6 -0.10435552628967315 1103 7 0.16375829630985095 1103 8 0.29550342421219966 1103 9 0.29424378759915726 1103 10-11 0.21496455496222922 1103 12-13 0.41844476417419685 1103 14-15 0.5784862323522049 1103 16-17 0.49542769725502467 1103 18-19 0.6098105626172128 1103 20-21 0.4311037595951106 1103 22-23 0.32588771976110564 1103 24-25 0.5490244800049808 1103 26-27 0.49388984094053257 1103 28-29 0.4843534193900396 1103 30-31 0.33612578820415706 1103 32-33 0.4293829069200612 1103 34-35 0.13800704806853048 1103 36-37 0.3391931922326634 1103 38-39 0.054761133966216846 1103 40-41 0.14181774624529453 1103 42-43 0.21623460339161937 1103 44-45 0.42026353692249074 1103 46-47 0.2093089408999802 1103 48-49 0.10477400175789597 1103 50-51 0.18887830226502444 1103 52-53 0.22665686004611274 1103 54-55 0.24064176314239205 1103 56-57 0.01728035643350978 1103 58-59 0.16355581312222967 1103 60-61 0.2919657434897118 1103 62-63 0.5128538810077075 1103 64-65 0.44831121074873437 1103 66-67 0.3609509384910723 1103 68-69 0.29355741175787387 1103 70-71 0.0644042468473387 1103 72-73 0.24528455035876817 1103 74-75 0.31620153746175106 1103 76-77 0.13642875365329132 1103 78-79 0.2377696432548717 1103 80-81 0.2794128792126038 1103 82-83 0.32476744399007984 1103 84-85 0.3315618605460635 1103 86-87 0.462361189087364 1103 88-89 0.42376022451777473 1103 90-91 0.23060459708236536 1103 92-93 -0.38421311381356205 1103 94-95 0.43999146693517943 1103 96-97 0.2322782458267838 1103 98-99 -0.00949026527813146 1103 100-101 -0.1432653997801978 1104 1 0.09788878852096516 1104 2 0.5370148227615879 1104 3 0.3924360000306102 1104 4 0.5229341347173815 1104 5 0.6411165959276843 1104 6 0.3428692725540827 1104 7 0.7475320521611835 1104 8 0.38431417555504055 1104 9 0.3746199983116725 1104 10-11 0.5692152614359074 1104 12-13 0.4088237792096834 1104 14-15 0.7357332500625802 1104 16-17 0.755695833674551 1104 18-19 0.5537079243964484 1104 20-21 0.6992833225521551 1104 22-23 0.7580500684894602 1104 24-25 0.6721935806801724 1104 26-27 0.7181008554582959 1104 28-29 0.6732407503217317 1104 30-31 1.0965880700601929 1104 32-33 1.0755366701057696 1104 34-35 0.9736305784954382 1104 36-37 1.23503955847724 1104 38-39 0.9577693096462738 1104 40-41 0.9421229860149154 1104 42-43 1.0040228554355437 1104 44-45 1.0652793921331423 1104 46-47 1.1565984548892416 1104 48-49 0.8977933947298311 1104 50-51 0.8077762033371414 1104 52-53 0.9392137554448681 1104 54-55 0.9104812695815525 1104 56-57 0.7774837777075376 1104 58-59 0.8580841475701035 1104 60-61 0.5874982412786096 1104 62-63 0.7130178260429787 1104 64-65 0.7074630109083699 1104 66-67 0.8195224595127044 1104 68-69 0.6626332794801115 1104 70-71 0.8793405024290237 1104 72-73 0.590205490022683 1104 74-75 0.7101765574155863 1104 76-77 0.7547873618462262 1104 78-79 0.22272876485462945 1104 80-81 0.36339156635801473 1104 82-83 0.660730728930865 1104 84-85 0.689900407043897 1104 86-87 0.64055434222497 1104 88-89 0.6367000178607434 1104 90-91 0.9939952392723228 1104 92-93 0.7129728559428976 1104 94-95 0.5399925455209349 1104 96-97 0.8308663770690998 1104 98-99 0.6485117624630945 1104 100-101 0.9496493703731765 1105 1 -0.8068989652516976 1105 2 -0.6315879348108773 1105 3 -0.703351645176209 1105 4 -0.43049111769821025 1105 5 -0.6770247568027443 1105 6 -0.8019792780918849 1105 7 -0.5748650817005085 1105 8 -0.6653872683707007 1105 9 -0.8194957328792967 1105 10-11 -0.4604666406377049 1105 12-13 -0.6824149570838784 1105 14-15 -0.7290697029284061 1105 16-17 -0.6739480703532479 1105 18-19 -0.7425736954059587 1105 20-21 -0.5833352856337086 1105 22-23 -0.7718001106441861 1105 24-25 -0.8903892007767809 1105 26-27 -0.7205865834734126 1105 28-29 -1.1605069426540595 1105 30-31 -0.9843206260640116 1105 32-33 -0.6917470124476992 1105 34-35 -0.917260747093529 1105 36-37 -0.9600912633662091 1105 38-39 -0.6885720213709945 1105 40-41 -0.7474384079705345 1105 42-43 -0.5530610659627868 1105 44-45 -0.4026285440301436 1105 46-47 -0.5053383597888583 1105 48-49 -0.7706465140748548 1105 50-51 -0.75648934872018 1105 52-53 -0.8593206056451379 1105 54-55 -0.9799595077499035 1105 56-57 -0.6858915344192624 1105 58-59 -0.7154637842505522 1105 60-61 -0.4975413852548769 1105 62-63 -0.37376971059799047 1105 64-65 -0.4618345680732787 1105 66-67 -0.6367689312157694 1105 68-69 -0.2707561462065655 1105 70-71 -0.37905706569604547 1105 72-73 -0.5715467627186044 1105 74-75 -0.4536601595532588 1105 76-77 -0.7583328205443962 1105 78-79 -0.576712121042668 1105 80-81 -0.5338834309278369 1105 82-83 -0.5149559500399299 1105 84-85 -0.9202830692344683 1105 86-87 -0.7201773915715748 1105 88-89 -0.8771475556926127 1105 90-91 -0.7257311747978115 1105 92-93 -0.6362129002182151 1105 94-95 -0.5082704009561034 1105 96-97 -0.4076907828483911 1105 98-99 -0.6839602818317694 1105 100-101 -0.9307273531096136 1106 1 0.469198337762581 1106 2 0.6839065125714576 1106 3 0.705638307229929 1106 4 0.6128721821431427 1106 5 0.8064231543553717 1106 6 0.7703633977410078 1106 7 0.5876944106315918 1106 8 0.5506888818143878 1106 9 0.6814561444342928 1106 10-11 0.5053186581460025 1106 12-13 0.7511651592566864 1106 14-15 0.787217975548586 1106 16-17 0.8494258059027047 1106 18-19 0.3548615240546411 1106 20-21 0.36370331827956903 1106 22-23 0.7055399210850553 1106 24-25 0.7563849927716149 1106 26-27 0.6241252092288576 1106 28-29 0.46265540530998095 1106 30-31 0.697752351196705 1106 32-33 0.5742762556644081 1106 34-35 0.56795871350932 1106 36-37 0.6284597678342152 1106 38-39 0.5969382906332399 1106 40-41 0.5308006189993222 1106 42-43 0.6789854702614306 1106 44-45 0.9129187854251697 1106 46-47 0.9768932636907763 1106 48-49 1.0289833114143008 1106 50-51 0.6961547549286777 1106 52-53 0.552736506993682 1106 54-55 0.7291097677656921 1106 56-57 0.6380904856759244 1106 58-59 0.5737218318256012 1106 60-61 0.6788996512337491 1106 62-63 0.6842419234580603 1106 64-65 0.7890908682038216 1106 66-67 0.8825859074939117 1106 68-69 0.9416975819795823 1106 70-71 1.119673764552502 1106 72-73 0.8459307624003785 1106 74-75 0.9233361387016359 1106 76-77 0.9730526897676057 1106 78-79 1.3720451502423643 1106 80-81 1.3780679175650299 1106 82-83 1.3874237432440495 1106 84-85 1.5717846198189456 1106 86-87 1.4059463524791944 1106 88-89 1.5444547711185983 1106 90-91 1.57987432493778 1106 92-93 1.3489801193481519 1106 94-95 1.8047650300327938 1106 96-97 1.7259422155651478 1106 98-99 1.5681550010873195 1106 100-101 1.5946824829559567 1107 1 0.4648753182321883 1107 2 0.7052582790845143 1107 3 0.6710322067252115 1107 4 0.8940878942344312 1107 5 0.8307593404427109 1107 6 0.9091169636835161 1107 7 0.5895377286039434 1107 8 0.8653476485468872 1107 9 0.9150308646449616 1107 10-11 0.8151606594626628 1107 12-13 0.7655386505887023 1107 14-15 0.6389296279514838 1107 16-17 0.8456404207809101 1107 18-19 0.8075935846208111 1107 20-21 0.7997248236559074 1107 22-23 0.8899485232355957 1107 24-25 0.8895427720123337 1107 26-27 0.5467826593056628 1107 28-29 0.48882393723888384 1107 30-31 0.8572651885897216 1107 32-33 0.8030561547208066 1107 34-35 0.8911757422563014 1107 36-37 0.830412807114449 1107 38-39 0.8960605201702165 1107 40-41 0.7482792233442837 1107 42-43 0.8994704384422576 1107 44-45 0.6272922767571885 1107 46-47 0.7134304592141447 1107 48-49 1.065586339722401 1107 50-51 1.058125349618166 1107 52-53 0.9028462283015557 1107 54-55 0.9979818327207113 1107 56-57 1.2526943917544813 1107 58-59 1.1681260451238202 1107 60-61 1.220714441666054 1107 62-63 1.2144481995168732 1107 64-65 1.2710526851886712 1107 66-67 1.2496256266073615 1107 68-69 1.3309668380691164 1107 70-71 1.2517782192376004 1107 72-73 1.1317876858149063 1107 74-75 0.9088858424541044 1107 76-77 0.9668205194801374 1107 78-79 1.2302523040716409 1107 80-81 1.4720661620241025 1107 82-83 1.4012925165598276 1107 84-85 1.3280497064987777 1107 86-87 1.4423518824331047 1107 88-89 1.1557341215684538 1107 90-91 1.5786125298971818 1107 92-93 1.7702441088149037 1107 94-95 1.359450129879182 1107 96-97 1.4789156630086424 1107 98-99 1.586983177519187 1107 100-101 1.4684920067654836 1108 1 -0.09438765075909217 1108 2 0.2566751648572314 1108 3 0.42089656964829203 1108 4 0.2518985031405734 1108 5 0.10349420125820075 1108 6 0.2894015928726148 1108 7 0.19646147969947236 1108 8 0.3620088897304754 1108 9 0.2622200470663927 1108 10-11 0.35779243609970024 1108 12-13 0.3271596179940701 1108 14-15 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-1.3817300142001017 2316 92-93 -1.634555421616735 2316 94-95 -1.3549409362817961 2316 96-97 -1.3781970935984234 2316 98-99 -1.198166723149047 2316 100-101 -1.175892738282979 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9957.0 3 1215.0 4 904.0 5 813.0 6 719.0 7 761.0 8 796.0 9 811.0 10 821.0 11 881.0 12 923.0 13 1021.0 14 978.0 15 1095.0 16 1189.0 17 1446.0 18 1326.0 19 1073.0 20 1084.0 21 1116.0 22 1308.0 23 1458.0 24 1683.0 25 1843.0 26 2186.0 27 2643.0 28 3090.0 29 3722.0 30 4612.0 31 5807.0 32 7376.0 33 9734.0 34 13602.0 35 21173.0 36 36578.0 37 68570.0 38 94999.0 39 31848.0 40 38.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.79325850611689 21.472515086763565 14.517340972597271 23.216885434522272 2 15.98592231442317 22.648166466577237 38.89034023030342 22.47557098869617 3 18.488058444207677 26.515918650077342 31.029553772956348 23.966469132758636 4 13.88618191637999 19.14266593089197 37.57734440126334 29.393807751464703 5 14.165844896809185 37.1842055833392 34.69424291516987 13.955706604681739 6 29.5956691411637 37.199612687421144 19.007364807370674 14.197353364044481 7 25.384477694278246 33.092811638941626 23.9768535771611 17.54585708961903 8 23.925321941300908 34.63909419258677 22.6316885890917 18.803895277020626 9 25.444495299792276 17.447980847093618 22.42016688377988 34.687356969334225 10-11 23.70899938101203 26.77210975155407 29.608453439490255 19.910437427943638 12-13 24.308726013731587 25.41928290593275 29.002699372102576 21.269291708233084 14-15 22.258303557671848 27.40588567613374 26.38708115365709 23.948729612537324 16-17 21.87887984549593 29.64003839120877 27.371200136189 21.109881627106304 18-19 21.712683183108265 29.00302602498892 28.682226285620366 20.60206450628245 20-21 22.60275982696789 27.905976886008933 28.411338651077344 21.079924635945837 22-23 22.540338377250578 27.909740634174902 27.9800219207186 21.56989906785592 24-25 22.284848315917184 28.08726510936012 28.210789455539302 21.41709711918339 26-27 22.12448193654869 28.31718320190721 28.254538785989364 21.30379607555474 28-29 22.16955110712043 28.012817127062096 28.41525965832732 21.402372107490155 30-31 22.466490531383815 28.04042407429955 27.917491231132818 21.575594163183816 32-33 22.246818823294692 28.18711803141128 27.93699553740395 21.629067607890082 34-35 22.446952423711554 28.08007535675614 27.960936560057988 21.51203565947432 36-37 22.555162001238376 27.99212956577623 27.822513198090203 21.630195234895194 38-39 22.183328981072066 28.32471194387637 27.895891954380474 21.59606712067109 40-41 22.261899871479628 28.017147786691392 28.08331523876034 21.637637103068645 42-43 22.470080479768566 27.867330143091447 27.82933477295199 21.833254604188 44-45 22.377280697249592 28.173901560448105 27.511573140044167 21.937244602258136 46-47 22.417023574240442 28.266492944747917 27.54358087239509 21.772902608616548 48-49 22.34348219107323 28.067402737667585 27.716389612829 21.872725458430185 50-51 22.25450191697369 28.0666076828608 27.660470432533234 22.01841996763227 52-53 22.391886995339394 28.175762207868605 27.539384835206427 21.892965961585574 54-55 22.49586696845987 28.140700569016126 27.55452154383947 21.80891091868453 56-57 22.375787879854926 28.25143490967992 27.456219115696562 21.916558094768597 58-59 22.1984722073479 28.11848535217425 27.73352972329847 21.949512717179378 60-61 22.320609925519726 28.025972006448992 27.747386148752234 21.90603191927905 62-63 22.110955278708534 28.20445863236528 27.698182629055633 21.986403459870548 64-65 22.257161849057876 28.15392087666344 27.519431600911524 22.06948567336715 66-67 22.221863569471935 28.02250122145715 27.68504158823176 22.07059362083916 68-69 22.008630720493485 28.129956758358905 27.6275496354467 22.233862885700912 70-71 22.2307802904266 27.906099897596604 27.628525068293385 22.23459474368341 72-73 22.22551515195974 27.953696843433416 27.479808390613194 22.340979613993646 74-75 22.20729330002684 28.03040415167459 27.417568635964262 22.344733912334306 76-77 22.168519839068924 27.973219237778306 27.67095992276247 22.187301000390296 78-79 22.063378638725325 27.756363321560677 27.80947965072345 22.37077838899054 80-81 22.031147747848213 28.15960066086987 27.59322461652233 22.216026974759583 82-83 22.000730750662882 27.954000859879297 27.595668908320135 22.449599481137682 84-85 22.143545696372843 27.82879688006655 27.73474696758726 22.292910455973345 86-87 22.057209433364243 27.982996231819406 27.556728139270074 22.40306619554627 88-89 22.060036092078818 27.724145013864643 27.63244765914993 22.583371234906615 90-91 22.00362716500079 27.648476062471754 27.881043074051686 22.46685369847577 92-93 22.072225434459117 27.684166346258266 27.696640261101052 22.54696795818157 94-95 22.104934214387157 27.4270548467741 27.844890318188753 22.623120620649992 96-97 21.837525908692854 27.36720740596226 28.142451237383035 22.65281544796185 98-99 21.93331563220178 27.52794896206335 27.70266206437246 22.836073341362408 100-101 22.03954996286871 27.530431515514426 27.759530120375835 22.670488401241037 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 692.0 1 399.0 2 95.5 3 82.5 4 75.5 5 73.0 6 98.5 7 152.0 8 204.0 9 224.5 10 167.5 11 116.0 12 101.5 13 92.0 14 107.0 15 115.0 16 116.0 17 120.0 18 125.5 19 138.0 20 164.5 21 183.5 22 207.5 23 248.5 24 292.5 25 365.0 26 505.0 27 755.0 28 1172.5 29 1931.5 30 3106.5 31 4776.0 32 7062.5 33 9533.0 34 11606.5 35 12730.5 36 14000.5 37 15078.5 38 15053.5 39 14428.5 40 13368.0 41 12352.0 42 11488.5 43 11026.0 44 10839.0 45 10944.0 46 11144.0 47 11170.5 48 11240.0 49 11195.5 50 10908.0 51 10676.0 52 10258.5 53 9820.5 54 9462.0 55 8835.5 56 7918.5 57 6887.5 58 6271.5 59 5738.5 60 4789.5 61 3895.0 62 3197.0 63 2473.0 64 1877.5 65 1473.0 66 1177.0 67 924.5 68 725.0 69 596.0 70 485.5 71 379.5 72 304.0 73 240.0 74 206.0 75 175.0 76 142.5 77 110.5 78 75.0 79 51.5 80 44.0 81 40.0 82 28.5 83 22.0 84 16.0 85 12.5 86 9.5 87 6.0 88 5.5 89 4.0 90 3.0 91 5.0 92 4.0 93 1.0 94 0.5 95 2.0 96 2.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14742129959349237 2 0.1518175610127814 3 0.145955879120396 4 0.15093830872892358 5 0.13804260856567574 6 0.11400971280689567 7 0.12075064698313888 8 0.11019961957684518 9 0.10638952634679469 10-11 0.09378691027816612 12-13 0.11107887186070299 14-15 0.13379288919369633 16-17 0.1453697109311575 18-19 0.14302503817420334 20-21 0.1327670948625289 22-23 0.12470728226049901 24-25 0.11005307752953554 26-27 0.11327700257034751 28-29 0.118699058320804 30-31 0.10624298429948505 32-33 0.1072687786306525 34-35 0.12309531974009301 36-37 0.12602616068628572 38-39 0.11137195595532226 40-41 0.11635438556384983 42-43 0.10785494681989102 44-45 0.12646578682821463 46-47 0.12441419816587973 48-49 0.13804260856567574 50-51 0.12529345044973753 52-53 0.10653606839410432 54-55 0.10184672288019601 56-57 0.12045756288851961 58-59 0.1115184980026319 60-61 0.10858765705643922 62-63 0.11049270367146445 64-65 0.13262055281521928 66-67 0.12236260950354486 68-69 0.12881045958516876 70-71 0.11430279690151496 72-73 0.11767326398963655 74-75 0.09510578870395282 76-77 0.12661232887552423 78-79 0.12822429139593025 80-81 0.12778466525400134 82-83 0.13291363690983854 84-85 0.1238280299766412 86-87 0.13218092667329037 88-89 0.11840597422618472 90-91 0.1280777493486206 92-93 0.14302503817420334 94-95 0.11576821737461129 96-97 0.09920896602862259 98-99 0.10389831154253089 100-101 0.15123139282354287 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 341199.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.05493074909678 #Duplication Level Percentage of deduplicated Percentage of total 1 70.69801760885225 43.164625692068995 2 16.72634799068573 20.424520365132224 3 6.221128947530655 11.394917912180562 4 2.6829732644443345 6.552349874493078 5 1.3149052162638417 4.014072346030749 6 0.7485212172736655 2.7420546650923963 7 0.448415993335217 1.9164605193908384 8 0.2712381954665564 1.3248343392576463 9 0.18824248311124184 1.0343818593355403 >10 0.6851971259374294 6.477974940720648 >50 0.012106417015393119 0.44763314763051093 >100 0.002905540083685979 0.506174338666785 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 456 0.1336463471463867 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 388 0.11371662871227642 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 347 0.10170018083288637 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 4.3962614192890367E-4 0.0 26-27 0.0 0.0 0.0 0.0017585045677156147 0.0 28-29 0.0 0.0 0.0 0.005715139845075747 0.0 30-31 0.0 0.0 0.0 0.01348186835248638 0.0 32-33 0.0 0.0 0.0 0.02828261513075947 0.0 34-35 0.0 0.0 0.0 0.05729794049806711 0.0 36-37 0.0 0.0 0.0 0.09613158303512026 0.0 38-39 0.0 0.0 0.0 0.1386287767549143 0.0 40-41 0.0 0.0 0.0 0.18713419441440332 0.0 42-43 0.0 0.0 0.0 0.23549307002658273 0.0 44-45 0.0 0.0 0.0 0.29865269241703524 0.0 46-47 0.0 0.0 0.0 0.3799835286738824 0.0 48-49 0.0 0.0 0.0 0.4450481976793601 0.0 50-51 0.0 0.0 0.0 0.5139229599148883 0.0 52-53 0.0 0.0 0.0 0.5942280018405681 0.0 54-55 0.0 0.0 0.0 0.6743865017189382 0.0 56-57 0.0 0.0 0.0 0.7734489257002513 0.0 58-59 0.0 0.0 0.0 0.8695805087353714 0.0 60-61 0.0 0.0 0.0 0.9649793815339436 0.0 62-63 0.0 0.0 0.0 1.069463861265713 0.0 64-65 0.0 0.0 0.0 1.191386844627329 0.0 66-67 0.0 0.0 0.0 1.3476006670593992 0.0 68-69 0.0 0.0 0.0 1.509383087289236 0.0 70-71 0.0 0.0 0.0 1.6743894325598845 0.0 72-73 0.0 0.0 0.0 1.8439385812971316 0.0 74-75 0.0 0.0 0.0 2.031512401853464 0.0 76-77 0.0 0.0 0.0 2.2379901465127388 0.0 78-79 0.0 0.0 0.0 2.4750951790597275 0.0 80-81 0.0 0.0 0.0 2.715717220742148 0.0 82-83 0.0 0.0 0.0 2.9607355238438564 0.0 84-85 0.0 0.0 0.0 3.2302263488462746 0.0 86-87 0.0 0.0 0.0 3.55672203025214 0.0 88-89 0.0 0.0 0.0 3.933042007743282 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 25 0.0050176345 57.001762 2 GGTATCA 480 0.0 40.574173 1 GTATCAA 750 0.0 39.901234 1 TCAACGC 840 0.0 34.495113 4 CAACGCA 870 0.0 33.30563 5 ATCAACG 880 0.0 32.927155 3 AACGCAG 905 0.0 32.012863 6 TATCAAC 910 0.0 31.841642 2 ACCGTCC 60 0.003943646 31.672298 8 GTATATG 90 2.407504E-5 31.667645 1 ATACTGC 135 1.19325705E-8 31.662994 6 ACGCAGA 1070 0.0 27.520174 7 CGGATAC 70 0.008382155 27.143698 3 CGCAGAG 1055 0.0 26.568707 8 TAACCGT 90 8.93295E-4 26.389704 5 TTAACCG 95 0.0012245607 25.000772 4 GTGTTAT 100 0.0016509667 23.750734 1 GTGGTAT 290 3.6379788E-12 22.931744 1 TACTCTC 125 2.2509047E-4 22.800705 5 GCAGAGT 1220 0.0 22.196568 9 >>END_MODULE