FastQCFastQC Report
Fri 10 Feb 2017
SRR2031751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341199
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT57361.6811303667361277No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT27210.7974818214590312RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT18340.5375162295317396RNA PCR Primer, Index 46 (95% over 23bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG11010.3226855881758153RNA PCR Primer, Index 46 (96% over 28bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT7710.2259678369514565RNA PCR Primer, Index 46 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.15357606558049702No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG4940.14478354274191893TruSeq Adapter, Index 4 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.14126653360648772No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA200.002073292471.245939
GGTATCA3950.054.158461
GTATCAA5600.038.173062
TCAACGC6600.032.3845185
CTAGGGC1201.4993748E-731.6694984
ATCAACG6850.031.2071724
CAACGCA6850.031.2025996
CGTCAGA1004.940027E-528.502552
AACGCAG7550.028.309647
TATACAC22350.027.8436553
CTTATAC22600.027.1351471
TTATACA23150.026.6762522
TATCAAC8800.024.8317663
TACACAT25800.024.116845
ACGCAGA8750.023.8843528
TACGGAT1201.7063472E-423.7521254
ACATCTC26650.023.3476358
ATACACA26900.023.3106374
CACATCT26900.022.777517
ACACATC27000.022.693156
Produced by FastQC (version 0.11.5)