##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031751_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 341199 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70634146055528 34.0 31.0 34.0 31.0 34.0 2 32.88720365534483 34.0 31.0 34.0 31.0 34.0 3 32.95633926242457 34.0 31.0 34.0 31.0 34.0 4 36.3588375112471 37.0 37.0 37.0 35.0 37.0 5 36.31269435139024 37.0 37.0 37.0 35.0 37.0 6 36.330900735347996 37.0 37.0 37.0 35.0 37.0 7 36.27556352744293 37.0 37.0 37.0 35.0 37.0 8 36.26454356548525 37.0 37.0 37.0 35.0 37.0 9 38.11588252017151 39.0 39.0 39.0 37.0 39.0 10-11 38.07149933030284 39.0 39.0 39.0 35.0 39.0 12-13 38.016754152268916 39.0 39.0 39.0 35.0 39.0 14-15 39.504425569828754 41.0 39.0 41.0 36.5 41.0 16-17 39.462603934947055 41.0 39.0 41.0 36.0 41.0 18-19 39.458150522129316 41.0 39.0 41.0 36.0 41.0 20-21 39.468616555148174 41.0 39.0 41.0 36.0 41.0 22-23 39.3789694577065 41.0 39.0 41.0 36.0 41.0 24-25 39.309410344110034 41.0 39.0 41.0 36.0 41.0 26-27 39.18581971225004 41.0 39.0 41.0 36.0 41.0 28-29 39.06496208957236 40.5 39.0 41.0 35.5 41.0 30-31 38.91214511179693 40.0 38.5 41.0 35.0 41.0 32-33 38.69584025744507 40.0 38.0 41.0 35.0 41.0 34-35 38.50417498292785 40.0 38.0 41.0 34.0 41.0 36-37 38.40253634975484 40.0 38.0 41.0 34.0 41.0 38-39 38.37506118130475 40.0 38.0 41.0 34.0 41.0 40-41 38.34920530247744 40.0 38.0 41.0 34.0 41.0 42-43 38.251822250358295 40.0 38.0 41.0 34.0 41.0 44-45 38.0904296319743 40.0 38.0 41.0 33.5 41.0 46-47 37.89729600614304 40.0 38.0 41.0 33.0 41.0 48-49 37.74431783211557 40.0 38.0 41.0 33.0 41.0 50-51 37.49910169724999 40.0 37.0 41.0 32.5 41.0 52-53 37.64796936685043 40.0 37.0 41.0 33.0 41.0 54-55 37.59687162037403 40.0 37.0 41.0 33.0 41.0 56-57 37.31157037388738 40.0 36.5 41.0 32.5 41.0 58-59 37.0710934088318 40.0 36.0 41.0 32.0 41.0 60-61 36.70027315437619 39.5 36.0 41.0 31.5 41.0 62-63 36.343819882238805 39.0 35.0 41.0 31.0 41.0 64-65 35.84930495106961 39.0 35.0 41.0 30.5 41.0 66-67 35.30774123019118 38.5 35.0 40.5 29.0 41.0 68-69 34.70706830910993 37.5 35.0 40.0 27.5 41.0 70-71 34.341321340332186 37.0 35.0 40.0 26.0 41.0 72-73 33.97039997186393 36.5 34.5 39.0 26.0 41.0 74-75 33.59969695104616 36.0 34.0 39.0 26.0 41.0 76-77 32.69619635462003 35.0 33.5 37.0 25.0 39.0 78-79 32.890720664480256 35.0 34.0 37.0 26.0 39.0 80-81 32.651596282521346 35.0 34.0 37.0 26.0 39.0 82-83 32.36972998162362 35.0 34.0 36.5 25.5 38.5 84-85 32.0619858205915 35.0 34.0 36.0 25.0 37.0 86-87 31.803361088397093 35.0 34.0 36.0 24.0 37.0 88-89 31.571267793868095 35.0 33.5 35.0 23.5 36.5 90-91 31.357898176723847 35.0 33.0 35.0 22.0 36.0 92-93 31.18802810090299 35.0 33.0 35.0 20.0 36.0 94-95 31.013660649650205 35.0 33.0 35.0 19.5 36.0 96-97 30.847690057708256 35.0 33.0 35.0 16.5 35.5 98-99 30.646562563196255 35.0 33.0 35.0 2.0 35.0 100-101 29.976176659368875 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21890301517309751 1101 2 0.18738170130720277 1101 3 -0.0359446529781593 1101 4 0.08168022155829391 1101 5 0.18898753350005393 1101 6 0.2257159920408469 1101 7 0.22471209659317992 1101 8 0.13706805770009112 1101 9 0.09818180903303642 1101 10-11 0.1579597640410384 1101 12-13 0.2157990430492447 1101 14-15 0.2711773302561227 1101 16-17 0.37026570545693005 1101 18-19 0.26556344975781343 1101 20-21 0.25032048350307434 1101 22-23 0.08891104902813396 1101 24-25 0.08393181198960775 1101 26-27 0.27380746497007635 1101 28-29 0.16258862271734387 1101 30-31 0.14152910559865006 1101 32-33 0.17321223848621514 1101 34-35 0.07921709230186735 1101 36-37 0.10154400810309028 1101 38-39 0.1127002663760166 1101 40-41 -0.27602386795883405 1101 42-43 0.05321426132546492 1101 44-45 0.13033481782589007 1101 46-47 0.06050354829925908 1101 48-49 -0.2555479982813367 1101 50-51 -0.3384913066803392 1101 52-53 -0.5471784310888737 1101 54-55 -0.4556974633800266 1101 56-57 -0.2690085593157221 1101 58-59 -0.5373212212841096 1101 60-61 -0.34701568559700036 1101 62-63 -0.4286725117795598 1101 64-65 -0.17727156904732766 1101 66-67 0.010036048422328747 1101 68-69 0.05202004698140428 1101 70-71 -0.15995268449031386 1101 72-73 -0.20033780520474664 1101 74-75 -0.10752199808158736 1101 76-77 0.07576940594800163 1101 78-79 8.654516276536128E-4 1101 80-81 -0.10071367540168552 1101 82-83 0.04123677069456022 1101 84-85 0.3456502910942305 1101 86-87 0.23913029390410756 1101 88-89 0.30791086717055904 1101 90-91 0.25602162390542205 1101 92-93 -0.0719335540047581 1101 94-95 -0.05060412724062502 1101 96-97 0.08973723016420365 1101 98-99 0.09024294997442084 1101 100-101 0.008380912067636359 1102 1 0.1280709323036433 1102 2 -0.07219039801996985 1102 3 0.15437191607165346 1102 4 -0.018312517683689578 1102 5 0.0771960624704846 1102 6 0.07451070620901135 1102 7 0.0341535022759345 1102 8 0.031859673944822475 1102 9 0.008475143657172168 1102 10-11 0.02522705674639525 1102 12-13 0.06898651582139337 1102 14-15 0.12835821994101337 1102 16-17 0.0370755704068344 1102 18-19 0.026666358901529463 1102 20-21 0.021642909564363322 1102 22-23 0.2151332766286984 1102 24-25 0.12394463932877642 1102 26-27 0.037615006477402346 1102 28-29 0.15645933281947322 1102 30-31 -0.28220873263166624 1102 32-33 -0.13676767341658547 1102 34-35 -0.19250467545067806 1102 36-37 0.05177756248617271 1102 38-39 0.1004961422654631 1102 40-41 0.12740199970175325 1102 42-43 0.042897934300917484 1102 44-45 -0.055123093980107285 1102 46-47 -0.1749507331277229 1102 48-49 -0.04457457265570497 1102 50-51 -0.2395634786150893 1102 52-53 -0.2813620798618075 1102 54-55 -0.19385032653893575 1102 56-57 -0.251830815959309 1102 58-59 -0.33847326155711954 1102 60-61 -0.3781280337261421 1102 62-63 -0.40618836443457695 1102 64-65 -0.11392145529544706 1102 66-67 0.027443715782801803 1102 68-69 0.239438363453651 1102 70-71 0.09696923815840108 1102 72-73 -0.055727707910584456 1102 74-75 0.042648629731957044 1102 76-77 -0.11496465668813016 1102 78-79 -0.1752260823284928 1102 80-81 -0.43018872259661833 1102 82-83 -0.24080132408253263 1102 84-85 -0.09237308854660853 1102 86-87 -0.23396254698848296 1102 88-89 -0.047158352055930663 1102 90-91 0.05257518409710826 1102 92-93 0.01106901146307493 1102 94-95 0.04629673920983279 1102 96-97 0.13244258860362734 1102 98-99 -0.1278581197772617 1102 100-101 -0.22258985128005904 1103 1 0.29796518066382305 1103 2 0.2488796122428525 1103 3 0.1384087729722765 1103 4 0.21134033136365815 1103 5 0.1649431509538175 1103 6 0.3047436275422797 1103 7 0.37330615052902516 1103 8 0.32298903006458346 1103 9 0.4178834699255205 1103 10-11 0.3777604109028374 1103 12-13 0.22942112382639124 1103 14-15 0.3747327681539474 1103 16-17 0.36936217073702693 1103 18-19 0.35018494352515717 1103 20-21 0.3022305674109518 1103 22-23 0.26682106387603 1103 24-25 0.12081559555563359 1103 26-27 0.4613801218225433 1103 28-29 0.3560543977429518 1103 30-31 0.14657623903404016 1103 32-33 0.28112421003350363 1103 34-35 0.39437230460375616 1103 36-37 0.21201695385647668 1103 38-39 -0.09143527534197204 1103 40-41 -0.19415326333872684 1103 42-43 -0.09727318702342558 1103 44-45 -0.4475475730305689 1103 46-47 -0.4867507420519175 1103 48-49 -0.21446882101588471 1103 50-51 -0.4458795733899805 1103 52-53 -0.6009819094097892 1103 54-55 -0.12325999143564559 1103 56-57 -0.2460691364269394 1103 58-59 -0.33644300855635834 1103 60-61 -0.36729629931000574 1103 62-63 -0.3216986049414743 1103 64-65 -0.22831840322280783 1103 66-67 -0.502814862593894 1103 68-69 -0.4058418560814019 1103 70-71 -0.3698662444171177 1103 72-73 -0.5018479167366081 1103 74-75 -0.3683270072320326 1103 76-77 -0.33742795596081265 1103 78-79 -0.39690851506241387 1103 80-81 -0.674170472630081 1103 82-83 -0.3836720125837161 1103 84-85 -0.3060572168764466 1103 86-87 -0.05364373033594916 1103 88-89 -0.23111377573180292 1103 90-91 -0.46683492700904594 1103 92-93 -0.2512017484316189 1103 94-95 -0.43783493719907085 1103 96-97 -0.5761045534248446 1103 98-99 -0.4488277329136672 1103 100-101 -0.4542115995286835 1104 1 3.83369934056077E-4 1104 2 -0.0367191107116156 1104 3 -0.11472451865574129 1104 4 0.0026663446949797276 1104 5 -0.012343990146774786 1104 6 -0.06857647513908205 1104 7 0.07967196367482643 1104 8 0.17728287996859393 1104 9 -0.06285298843028642 1104 10-11 -0.14259250235729581 1104 12-13 -0.11864389332312442 1104 14-15 -0.09374556305817094 1104 16-17 -0.14280966954599705 1104 18-19 0.05772641514756316 1104 20-21 0.21952461146039326 1104 22-23 0.07963250551375012 1104 24-25 -0.04373884066759359 1104 26-27 -0.04716227738362022 1104 28-29 -0.0024243649034687564 1104 30-31 -0.00596814476991625 1104 32-33 -0.0847967943455572 1104 34-35 -0.07402513267648203 1104 36-37 -0.06755247470878345 1104 38-39 -0.13608872573780673 1104 40-41 0.10153562938513261 1104 42-43 0.2657841721672014 1104 44-45 -0.2752650698487358 1104 46-47 -0.33659003277391975 1104 48-49 -0.2728472751867699 1104 50-51 -0.2403119137053551 1104 52-53 -0.12103821158638794 1104 54-55 -0.20285422747335247 1104 56-57 -0.6314801854594521 1104 58-59 -0.5170090503623399 1104 60-61 -0.645069882587606 1104 62-63 -0.31195358261474837 1104 64-65 -0.167718062389703 1104 66-67 0.021286136176520642 1104 68-69 0.039436095468524 1104 70-71 0.32143172568448364 1104 72-73 0.10827029626251061 1104 74-75 0.22439361748483577 1104 76-77 -0.009106451117958159 1104 78-79 0.11769553610569261 1104 80-81 0.07333799426812249 1104 82-83 0.15851147642549535 1104 84-85 0.17451206068681557 1104 86-87 0.09574579012482332 1104 88-89 -0.07524734779211784 1104 90-91 -0.14435396404768142 1104 92-93 -0.38852728718520524 1104 94-95 -0.16094040131452658 1104 96-97 -0.21308494473575834 1104 98-99 -0.3718706390551354 1104 100-101 -0.7360730228304497 1105 1 0.1374803192338092 1105 2 0.05641952787269133 1105 3 -0.09693057662990157 1105 4 0.008691749102752055 1105 5 0.04029958818348689 1105 6 -0.05164020329020502 1105 7 -0.04856601265781535 1105 8 0.07118147946918896 1105 9 -0.24071641259703824 1105 10-11 0.0021583390280071058 1105 12-13 0.059105536706198336 1105 14-15 -0.039012092208103866 1105 16-17 0.07831181563476974 1105 18-19 0.03927022146609005 1105 20-21 -0.1875755948147173 1105 22-23 -0.05649820776385184 1105 24-25 -0.018784981151796387 1105 26-27 0.04783283715335074 1105 28-29 0.21636838289807514 1105 30-31 0.004519315874965457 1105 32-33 0.007343038463041296 1105 34-35 0.10852833600226575 1105 36-37 0.18981371788702006 1105 38-39 0.10563312856682927 1105 40-41 0.044598271877809736 1105 42-43 0.23307871130518976 1105 44-45 0.2187203823280015 1105 46-47 0.33206700772114317 1105 48-49 0.014868499430534143 1105 50-51 0.3181616505113425 1105 52-53 0.3563203814002591 1105 54-55 0.4153794062254832 1105 56-57 -0.03281554535521991 1105 58-59 -0.06304096872081999 1105 60-61 -0.07173908245733429 1105 62-63 -0.36931434691829423 1105 64-65 -0.6393986616731766 1105 66-67 -0.8224496145562981 1105 68-69 -1.092433408385567 1105 70-71 -1.1521324910129422 1105 72-73 -1.1677420802438476 1105 74-75 -1.3817674942294218 1105 76-77 -1.2221452091246903 1105 78-79 -1.0396489433210796 1105 80-81 -0.9171244962323684 1105 82-83 -0.8430301585761342 1105 84-85 -1.03572027022188 1105 86-87 -1.1357871596091904 1105 88-89 -1.1061017609937274 1105 90-91 -1.0971441062689422 1105 92-93 -1.0583785519772988 1105 94-95 -0.6852157242979118 1105 96-97 -0.894292196934213 1105 98-99 -1.0101509560674025 1105 100-101 -1.144535730462632 1106 1 0.026745258419424545 1106 2 -0.03644139227537835 1106 3 -0.08201783205031177 1106 4 0.07331364228096504 1106 5 0.08921550568744863 1106 6 -0.017689912438804356 1106 7 0.11848845587734758 1106 8 -0.011843375105108578 1106 9 -0.11015057441618126 1106 10-11 -0.009052660443181537 1106 12-13 0.05899977256024158 1106 14-15 0.025662683043087497 1106 16-17 0.12550906576697685 1106 18-19 0.28726070269296144 1106 20-21 0.36379933908268924 1106 22-23 0.22784869863487955 1106 24-25 0.019580962793206425 1106 26-27 0.29347006613272697 1106 28-29 0.025913597070470473 1106 30-31 0.11993444014783705 1106 32-33 0.11984964872216608 1106 34-35 0.18396461310433665 1106 36-37 0.15930076177914287 1106 38-39 0.22176216082489475 1106 40-41 -0.08763335160183061 1106 42-43 0.21438489850772413 1106 44-45 -0.0985720555355627 1106 46-47 0.17418757884752978 1106 48-49 -0.20776502780373107 1106 50-51 -0.05248879898628189 1106 52-53 0.06525745173342301 1106 54-55 0.2568125104031651 1106 56-57 0.13759693481399893 1106 58-59 -0.02702827702331234 1106 60-61 0.29893780807677217 1106 62-63 0.1380891432985223 1106 64-65 0.3194855265870089 1106 66-67 -0.17141841413324244 1106 68-69 0.02678922514167681 1106 70-71 -0.004908799844251632 1106 72-73 0.04217330843939493 1106 74-75 0.1539279050302298 1106 76-77 -0.006756525888306442 1106 78-79 0.04681324599673786 1106 80-81 0.09889877187973894 1106 82-83 0.2619936383567065 1106 84-85 0.22401003120592833 1106 86-87 0.220003427381819 1106 88-89 0.314231396066031 1106 90-91 0.258540716576114 1106 92-93 0.09487369551079894 1106 94-95 0.2538641276322835 1106 96-97 0.22654333981882857 1106 98-99 0.35457670125676444 1106 100-101 0.6186054646722177 1107 1 0.2753079092862194 1107 2 0.1296547235903205 1107 3 0.1773194512501064 1107 4 0.06082735744444534 1107 5 0.02342660948380626 1107 6 0.07844789752389403 1107 7 0.15098790727081024 1107 8 0.06717791082861879 1107 9 0.13667847978614134 1107 10-11 0.09074984120264418 1107 12-13 -0.0015334729960443383 1107 14-15 0.326266150236421 1107 16-17 0.16610594968183534 1107 18-19 0.2510746153548027 1107 20-21 0.12862268777701047 1107 22-23 0.19545896854929623 1107 24-25 0.27725048221387993 1107 26-27 0.17189885700391017 1107 28-29 0.22109683165462712 1107 30-31 0.09427062623517202 1107 32-33 0.18411341874630693 1107 34-35 0.5008836387791149 1107 36-37 0.44664989498081553 1107 38-39 0.7127759857096905 1107 40-41 0.3690267317863629 1107 42-43 -0.06607373655919702 1107 44-45 0.3904667858074262 1107 46-47 0.4224539821830504 1107 48-49 0.24696396056580738 1107 50-51 0.6755888836996959 1107 52-53 0.6349085379743684 1107 54-55 0.40770125299698634 1107 56-57 0.4623500618712697 1107 58-59 0.4017187056407252 1107 60-61 0.6350580626302076 1107 62-63 0.9141918424426976 1107 64-65 0.7964550240634196 1107 66-67 0.8027093014691289 1107 68-69 1.1190977614594217 1107 70-71 0.9410424838950533 1107 72-73 0.7902092970283761 1107 74-75 1.0214833142907054 1107 76-77 1.0921572331636966 1107 78-79 1.0954965903022114 1107 80-81 0.971556221956547 1107 82-83 0.877628924729315 1107 84-85 0.9828645407516845 1107 86-87 0.9715285535613241 1107 88-89 0.9936696229696942 1107 90-91 0.9308251142061295 1107 92-93 1.0184308603122076 1107 94-95 0.8353541429932676 1107 96-97 0.9679192450196155 1107 98-99 1.069630464697621 1107 100-101 1.184163946129317 1108 1 0.11239734628801301 1108 2 0.056097693416290895 1108 3 0.04299701061741246 1108 4 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82-83 -0.5395193505099769 2316 84-85 -0.3167336170955224 2316 86-87 -0.130753067051522 2316 88-89 -0.41823363390827595 2316 90-91 -0.3092868597870009 2316 92-93 -0.4664737209893417 2316 94-95 -0.3815126922364058 2316 96-97 -0.507134935944805 2316 98-99 -0.4642928391371832 2316 100-101 -0.288188711747992 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 6.0 7 19.0 8 61.0 9 146.0 10 246.0 11 413.0 12 525.0 13 715.0 14 808.0 15 1014.0 16 1188.0 17 1518.0 18 1647.0 19 1455.0 20 1599.0 21 2056.0 22 2563.0 23 2822.0 24 3432.0 25 3420.0 26 2241.0 27 1901.0 28 2272.0 29 2795.0 30 3743.0 31 4674.0 32 6148.0 33 8153.0 34 11683.0 35 18480.0 36 32492.0 37 68658.0 38 112061.0 39 40166.0 40 77.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.1769146207402 22.0599486991572 15.05489190179553 24.708244778307073 2 14.830348271829635 21.87345214962529 40.98575904384245 22.31044053470262 3 16.901672640732478 27.038045469332967 33.08000949598033 22.980272393954227 4 12.588087002403705 20.53028082312247 36.77551738289265 30.106114791581167 5 13.038725201422045 37.12701385408515 36.25655409306592 13.57770685142688 6 27.735427126105293 38.06752071371839 19.31600033997755 14.88105182019877 7 23.885767543281194 32.891069434552854 23.704055404617247 19.519107617548702 8 23.094733571903785 35.31282330839188 23.34971673422255 18.242726385481784 9 23.28611748569017 16.704621056919862 22.016477187799495 37.99278426959047 10-11 22.54230308749892 26.788658215085942 30.427302581928117 20.241736115487026 12-13 23.329967746048123 24.542898049082865 29.539842642919005 22.58729156195001 14-15 21.24745565997506 26.76711650256376 26.052429890518276 25.93299794694291 16-17 21.44703599711601 30.016207787944687 26.94258984624583 21.59416636869347 18-19 21.3610259130682 28.137286652784233 28.69781080514715 21.80387662900042 20-21 21.623597958962364 27.598849938012716 28.12552205604354 22.65203004698138 22-23 21.645432724011503 28.873326123464604 27.68487011978347 21.796371032740424 24-25 21.841212899217172 28.83478556502217 28.310311577700993 21.01368995805967 26-27 21.786291557553742 29.035444176923402 28.660882155109125 20.51738211041373 28-29 20.96612991154385 27.592427590815564 29.511029174702365 21.93041332293822 30-31 21.423714606432025 28.95319153924836 28.351343350947687 21.271750503371933 32-33 23.07794887426069 27.611240394140644 28.452247668509195 20.858563063089466 34-35 21.91944048318709 27.5285320451471 28.830006609966542 21.722020861699274 36-37 21.129831893980803 28.60982433206764 28.533912103017876 21.726431670933678 38-39 21.11582976503448 27.072470904076507 29.406739175671675 22.40496015521734 40-41 21.05589406768484 26.99436399286047 29.872303259974387 22.077438679480306 42-43 22.066154941837464 26.58126196149461 29.735139903692563 21.61744319297536 44-45 20.95111650385845 27.493925832139016 29.51913692595817 22.035820738044368 46-47 22.292855489025463 26.643102705459278 28.51942707921184 22.54461472630342 48-49 21.65862150826937 26.585511680866592 28.918314531988663 22.83755227887538 50-51 20.889129217846477 26.896913531399562 29.619518228365266 22.594439022388695 52-53 21.150120604104934 27.34372023364664 30.345194446642576 21.16096471560585 54-55 21.049592759650526 27.61394376888561 29.05474517803393 22.281718293429932 56-57 21.91844055814935 28.294631578638857 27.998763185120705 21.788164678091086 58-59 20.463191460676793 28.99885110698187 29.508525841300358 21.02943159104098 60-61 21.51553519562242 30.111696312644163 27.559768225022204 20.813000266711214 62-63 20.557726184230198 31.16853425408373 27.426569081193886 20.847170480492185 64-65 20.769103386827556 30.921526014135843 27.303595216148214 21.005775382888395 66-67 20.56996808108401 30.604202510720242 27.540396920073746 21.285432488122 68-69 20.69985799430512 30.236368405857846 27.644508014015916 21.41926558582112 70-71 20.959322858507793 29.615415051040593 27.876693659711783 21.54856843073983 72-73 21.05057208137376 29.39092789004782 27.725758537253803 21.83274149132462 74-75 21.111514945453052 29.081495072895358 27.829201521804126 21.977788459847467 76-77 21.10374480132945 29.28401227457773 27.742949005401634 21.86929391869118 78-79 21.232947976031546 28.64754680926206 28.003347025265352 22.116158189441045 80-81 21.281946441211513 28.748316487214097 27.80642863737684 22.16330843419755 82-83 21.271378951533716 28.68846452596251 27.69862090190963 22.34153562059414 84-85 21.459618044971744 28.414792843911936 27.73790124035734 22.387687870758985 86-87 21.301841916683642 28.642544479891907 27.764303833455212 22.29130976996924 88-89 21.491836094690164 28.105903627523105 27.772371972789912 22.629888304996822 90-91 21.463574043300245 28.08199906799258 27.860280950413102 22.594145938294076 92-93 21.428550493993242 27.953335150454723 27.936482815014113 22.68163154053793 94-95 21.58652282099303 27.896769920193197 27.82906749433615 22.68763976447762 96-97 21.47705591165273 27.762683946904886 27.959929542583655 22.800330598858732 98-99 21.393673486733547 27.747003947842753 27.834489550086605 23.02483301533709 100-101 21.451306580381953 27.900825332067196 27.62312571073518 23.02474237681567 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 412.0 1 248.0 2 82.5 3 76.5 4 56.0 5 48.0 6 85.0 7 148.5 8 205.5 9 205.0 10 136.5 11 80.5 12 73.5 13 82.5 14 100.5 15 99.0 16 94.0 17 117.0 18 123.5 19 132.0 20 140.0 21 161.0 22 193.0 23 229.0 24 278.0 25 331.5 26 442.5 27 678.0 28 1134.0 29 1911.0 30 3196.5 31 4927.0 32 7093.5 33 9466.0 34 11588.5 35 13182.5 36 14467.0 37 15432.5 38 15761.5 39 15567.0 40 14888.5 41 13858.0 42 13320.0 43 13242.5 44 12819.5 45 12556.5 46 12567.0 47 12526.5 48 12267.0 49 11730.0 50 11026.0 51 10370.5 52 9742.0 53 9060.5 54 8205.0 55 7282.5 56 6360.5 57 5439.5 58 4841.5 59 4271.5 60 3562.5 61 2883.0 62 2315.5 63 1767.0 64 1332.0 65 1052.5 66 792.0 67 571.5 68 422.0 69 344.0 70 266.0 71 191.0 72 168.0 73 143.0 74 106.5 75 91.0 76 79.5 77 47.5 78 25.0 79 24.5 80 19.5 81 12.0 82 7.0 83 5.0 84 5.0 85 4.0 86 2.5 87 1.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021688223001825914 2 0.0 3 5.861681892385382E-4 4 0.017291961582536878 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.4654204730963456E-4 12-13 1.4654204730963456E-4 14-15 1.4654204730963456E-4 16-17 0.0014654204730963456 18-19 1.4654204730963456E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.4654204730963456E-4 28-29 0.0038100932300504983 30-31 0.0 32-33 2.930840946192691E-4 34-35 0.013921494494415283 36-37 0.004542803466598672 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.930840946192691E-4 60-61 0.0017585045677156147 62-63 0.008206354649339536 64-65 0.0027842988988830563 66-67 0.00644785008162392 68-69 0.004542803466598672 70-71 0.0 72-73 0.009525233075126245 74-75 0.007327102365481728 76-77 0.0017585045677156147 78-79 1.4654204730963456E-4 80-81 0.006594392128933555 82-83 0.00996485921705515 84-85 0.002051588662334884 86-87 0.00161196252040598 88-89 5.861681892385382E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.792522838578073E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 341199.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.454803275508866 #Duplication Level Percentage of deduplicated Percentage of total 1 66.10974659185409 35.999932452531574 2 19.01485846874126 20.70900754453898 3 7.256130912123089 11.853935440830044 4 3.1293124672994264 6.8162437917755 5 1.5925580831194206 4.336121856054477 6 0.86493149536724 2.825980465620886 7 0.5337593161245048 2.034603098922104 8 0.35040518929374004 1.5264996519766447 9 0.2521072891140419 1.2355607549724292 >10 0.8745037303342926 7.535099839286803 >50 0.011927551145891778 0.4156879687731829 >100 0.006505936988667764 0.9657069217734374 >500 0.0010843228314446275 0.3823266474156532 >1k 0.001626484247166941 1.6698374654694474 >5k 5.421614157223138E-4 1.6934561000588315 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 5736 1.6811303667361277 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 2721 0.7974818214590312 RNA PCR Primer, Index 46 (95% over 21bp) ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 1834 0.5375162295317396 RNA PCR Primer, Index 46 (95% over 23bp) TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG 1101 0.3226855881758153 RNA PCR Primer, Index 46 (96% over 28bp) CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 771 0.2259678369514565 RNA PCR Primer, Index 46 (96% over 26bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 524 0.15357606558049702 No Hit CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG 494 0.14478354274191893 TruSeq Adapter, Index 4 (95% over 23bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 482 0.14126653360648772 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 4.3962614192890367E-4 0.0 26-27 0.0 0.0 0.0 0.0017585045677156147 0.0 28-29 0.0 0.0 0.0 0.005128971655837209 0.0 30-31 0.0 0.0 0.0 0.013042242210557477 0.0 32-33 0.0 0.0 0.0 0.0279895310361402 0.0 34-35 0.0 0.0 0.0 0.05715139845075747 0.0 36-37 0.0 0.0 0.0 0.0967177512243588 0.0 38-39 0.0 0.0 0.0 0.13892186084953356 0.0 40-41 0.0 0.0 0.0 0.18772036260364186 0.0 42-43 0.0 0.0 0.0 0.23651886435775016 0.0 44-45 0.0 0.0 0.0 0.29997157084282194 0.0 46-47 0.0 0.0 0.0 0.3804231548158113 0.0 48-49 0.0 0.0 0.0 0.44475511358474085 0.0 50-51 0.0 0.0 0.0 0.5136298758202691 0.0 52-53 0.0 0.0 0.0 0.5937883756986392 0.0 54-55 0.0 0.0 0.0 0.67365379148239 0.0 56-57 0.0 0.0 0.0 0.7728627575110126 0.0 58-59 0.0 0.0 0.0 0.8670892939311077 0.0 60-61 0.0 0.0 0.0 0.960436578067345 0.0 62-63 0.0 0.0 0.0 1.062283300947541 0.0 64-65 0.0 0.0 0.0 1.184938994545705 0.0 66-67 0.0 0.0 0.0 1.3404201067412274 0.0 68-69 0.0 0.0 0.0 1.5011767326398964 0.0 70-71 0.0 0.0 0.0 1.6672088722417122 0.0 72-73 0.0 0.0 0.0 1.8366114789316499 0.0 74-75 0.0 0.0 0.0 2.028141934765342 0.0 76-77 0.0 0.0 0.0 2.2362316419450234 0.0 78-79 0.0 0.0 0.0 2.47421592677587 0.0 80-81 0.0 0.0 0.0 2.712786379795955 0.0 82-83 0.0 0.0 0.0 2.959123561323451 0.0 84-85 0.0 0.0 0.0 3.2322779375086093 0.0 86-87 0.0 0.0 0.0 3.5606786655295 0.0 88-89 0.0 0.0 0.0 3.939489857824906 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCGTA 20 0.0020732924 71.24593 9 GGTATCA 395 0.0 54.15846 1 GTATCAA 560 0.0 38.17306 2 TCAACGC 660 0.0 32.384518 5 CTAGGGC 120 1.4993748E-7 31.669498 4 ATCAACG 685 0.0 31.207172 4 CAACGCA 685 0.0 31.202599 6 CGTCAGA 100 4.940027E-5 28.50255 2 AACGCAG 755 0.0 28.30964 7 TATACAC 2235 0.0 27.843655 3 CTTATAC 2260 0.0 27.135147 1 TTATACA 2315 0.0 26.676252 2 TATCAAC 880 0.0 24.831766 3 TACACAT 2580 0.0 24.11684 5 ACGCAGA 875 0.0 23.884352 8 TACGGAT 120 1.7063472E-4 23.752125 4 ACATCTC 2665 0.0 23.347635 8 ATACACA 2690 0.0 23.310637 4 CACATCT 2690 0.0 22.77751 7 ACACATC 2700 0.0 22.69315 6 >>END_MODULE