FastQCFastQC Report
Fri 10 Feb 2017
SRR2031750_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031750_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718190
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18160.2528578788342917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14690.20454197357245296No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10940.15232737854885198No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC9600.13366936326041856No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC9430.13130230161934864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.11821384313343267No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA7850.10930255224940474No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC7650.10651777384814602No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG7230.10066973920550273No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17200.052.202971
GCGTCAG1350.045.7479251
CGGTCTA308.29092E-539.5840328-29
GTATCAA29150.039.440161
CGTCAGA1700.036.3292352
GTGGTAT9050.030.9717161
ATCAACG37000.030.6872543
TCAACGC37700.030.1174644
TACGTCG404.4955616E-429.68595522-23
TATCAAC38250.029.68442
CAACGCA38650.029.3730935
AACGCAG40400.028.3319456
ACGCAGA46400.024.6700527
CGCAGAG46650.024.5412648
CGCTGTA601.7100907E-423.75207316-17
ACGATCT500.00165501823.74710774-75
GACGATC500.00165501823.74710772-73
GTACATG43750.023.3465231
GCAGAGT50300.022.2882299
CATTCCG1954.2133252E-721.9249929
Produced by FastQC (version 0.11.5)