##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031750_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 718190 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.382593742602932 33.0 31.0 34.0 30.0 34.0 2 31.697131678246702 33.0 31.0 34.0 30.0 34.0 3 31.79323020370654 34.0 31.0 34.0 30.0 34.0 4 35.17516813099598 37.0 35.0 37.0 33.0 37.0 5 35.1730154972918 37.0 35.0 37.0 33.0 37.0 6 35.200474804717416 37.0 37.0 37.0 33.0 37.0 7 35.180506551191186 37.0 36.0 37.0 33.0 37.0 8 35.17395257522383 37.0 37.0 37.0 33.0 37.0 9 36.89242540274858 39.0 38.0 39.0 34.0 39.0 10-11 36.816432977345826 39.0 38.0 39.0 34.0 39.0 12-13 36.76994040574221 39.0 38.0 39.0 34.0 39.0 14-15 38.086981857168716 41.0 38.5 41.0 34.0 41.0 16-17 38.007007894846765 41.0 38.0 41.0 34.0 41.0 18-19 38.049706205878664 41.0 38.5 41.0 34.0 41.0 20-21 38.06789428981189 41.0 39.0 41.0 34.0 41.0 22-23 37.96202467313664 41.0 39.0 41.0 34.0 41.0 24-25 37.88078642142051 41.0 38.5 41.0 34.0 41.0 26-27 37.77776354446595 40.5 38.0 41.0 33.5 41.0 28-29 37.67395953716983 40.0 38.0 41.0 33.0 41.0 30-31 37.51778011389743 40.0 38.0 41.0 33.0 41.0 32-33 37.3263293835893 40.0 38.0 41.0 32.5 41.0 34-35 37.189341956863785 40.0 38.0 41.0 32.0 41.0 36-37 37.04487391915788 40.0 38.0 41.0 31.0 41.0 38-39 36.864575530152194 40.0 37.5 41.0 31.0 41.0 40-41 36.79287375207117 40.0 37.5 41.0 31.0 41.0 42-43 36.77064425848313 40.0 38.0 41.0 31.0 41.0 44-45 36.631007115108815 40.0 37.5 41.0 30.0 41.0 46-47 36.55541987496345 40.0 37.5 41.0 30.0 41.0 48-49 36.5816427407789 40.0 38.0 41.0 30.0 41.0 50-51 36.071698993302604 39.5 37.5 40.5 29.5 41.0 52-53 36.129298653559644 39.5 37.0 40.5 30.0 41.0 54-55 36.390654979879976 40.0 38.0 41.0 30.0 41.0 56-57 36.30806193347165 40.0 37.0 41.0 29.0 41.0 58-59 36.17678191007951 40.0 37.0 41.0 28.5 41.0 60-61 36.00261490691878 40.0 37.0 41.0 28.0 41.0 62-63 35.75393141090798 40.0 36.0 41.0 28.0 41.0 64-65 35.53855038360322 39.0 36.0 41.0 27.5 41.0 66-67 35.293180773889915 39.0 35.0 41.0 26.5 41.0 68-69 34.968135173143594 39.0 35.0 41.0 26.0 41.0 70-71 34.62088096464724 38.0 35.0 40.0 26.0 41.0 72-73 34.25580974393962 37.0 35.0 39.5 26.0 41.0 74-75 33.81424274913324 37.0 35.0 39.0 25.0 41.0 76-77 33.40899692281987 36.0 34.0 39.0 24.0 40.5 78-79 32.97777398738495 36.0 34.0 38.0 22.5 39.5 80-81 32.56585513582757 35.5 34.0 37.0 22.0 39.0 82-83 32.19095434355811 35.0 34.0 37.0 20.5 39.0 84-85 31.81013172001838 35.0 34.0 36.0 19.5 37.5 86-87 31.498861721828487 35.0 34.0 36.0 18.0 37.0 88-89 31.193796209916595 35.0 33.5 36.0 11.5 37.0 90-91 30.936533507846114 35.0 33.0 35.0 6.0 36.0 92-93 30.702512566312535 35.0 33.0 35.0 2.0 36.0 94-95 30.44187819379273 35.0 33.0 35.0 2.0 36.0 96-97 30.18721368997062 35.0 32.5 35.0 2.0 36.0 98-99 29.94796014982108 35.0 32.0 35.0 2.0 35.0 100-101 29.400108606357648 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.007691068533681289 1101 2 0.1362243354178858 1101 3 0.04198010446291178 1101 4 0.22347437176055251 1101 5 0.19476465737616167 1101 6 0.16106500577807026 1101 7 0.06092176362384549 1101 8 0.1526197301020673 1101 9 0.10603507661390665 1101 10-11 0.11345251341732165 1101 12-13 0.12174137414669417 1101 14-15 0.30933090052732126 1101 16-17 0.3830173778905319 1101 18-19 0.2939386152226149 1101 20-21 0.29132439601096394 1101 22-23 0.30425999549697735 1101 24-25 0.258890856248847 1101 26-27 3.5111428444167814E-4 1101 28-29 0.17856358162821806 1101 30-31 0.10722267371067318 1101 32-33 0.0339835423785928 1101 34-35 0.1646079695340532 1101 36-37 0.013380133393241067 1101 38-39 0.015963067048851087 1101 40-41 -0.46093141656941583 1101 42-43 -0.14151000330815577 1101 44-45 -0.3251233297767655 1101 46-47 -0.18537094519974318 1101 48-49 0.04941606204397431 1101 50-51 0.07694495051999439 1101 52-53 -0.1051645224130695 1101 54-55 0.17625199969020144 1101 56-57 0.11575164596906973 1101 58-59 -0.09101624884188908 1101 60-61 -0.36021872522581333 1101 62-63 -0.08666089804796684 1101 64-65 0.12250984984247992 1101 66-67 -0.304824632871437 1101 68-69 -0.4090579509024863 1101 70-71 -0.342841043312589 1101 72-73 0.061141734886220434 1101 74-75 0.19985494095797662 1101 76-77 -0.08023765520931647 1101 78-79 -0.24508224400198486 1101 80-81 -0.7143789437638404 1101 82-83 -0.4711811542846789 1101 84-85 -0.6326600874900521 1101 86-87 -0.5145502282956755 1101 88-89 -0.43160467526501733 1101 90-91 -0.9940541057319905 1101 92-93 -0.5113601785049902 1101 94-95 -0.46480154665714934 1101 96-97 -0.6008322686616197 1101 98-99 -0.5558779796813731 1101 100-101 -0.3328487312186077 1102 1 0.18020047633720537 1102 2 0.14454281432460192 1102 3 0.1028832536013553 1102 4 0.1839615969536581 1102 5 0.18723841455580015 1102 6 0.17582040288640854 1102 7 0.15539591692153465 1102 8 0.13390315256196317 1102 9 0.2654725889925942 1102 10-11 0.37221662512261133 1102 12-13 0.4129376506370903 1102 14-15 0.46282664225835646 1102 16-17 0.45040695998599745 1102 18-19 0.426836218708452 1102 20-21 0.34668189254492887 1102 22-23 0.5617367235619426 1102 24-25 0.30553375583292564 1102 26-27 0.2729298427455191 1102 28-29 0.3152738212796322 1102 30-31 0.21308522074969005 1102 32-33 0.2985408804653389 1102 34-35 0.5324530873791744 1102 36-37 0.2654102383645238 1102 38-39 0.23031244316292998 1102 40-41 0.11210284764132439 1102 42-43 0.22064091766629446 1102 44-45 0.0659442083495918 1102 46-47 0.080572806038127 1102 48-49 0.3102597934759359 1102 50-51 0.28183700835322867 1102 52-53 0.18464534092620966 1102 54-55 0.3433642991554464 1102 56-57 0.15932463071852254 1102 58-59 0.11486610409928488 1102 60-61 0.05095180359177931 1102 62-63 0.0793620767985459 1102 64-65 0.25753658781039945 1102 66-67 0.08693611183047523 1102 68-69 -0.19366876492507146 1102 70-71 -0.11967804073781707 1102 72-73 0.10738851642711467 1102 74-75 -0.13649879245458152 1102 76-77 0.0953911821842297 1102 78-79 -0.06752233114795558 1102 80-81 -0.08866097981058374 1102 82-83 0.05625002020531156 1102 84-85 -0.040364583429841616 1102 86-87 -0.05584553429686423 1102 88-89 -0.18873480004220156 1102 90-91 -0.17943734993876248 1102 92-93 0.037213796539571575 1102 94-95 -0.33626124164625537 1102 96-97 -0.4259461525990318 1102 98-99 -0.1954105603904246 1102 100-101 -0.3378992362691129 1103 1 -0.17888738192693054 1103 2 0.06290268985086911 1103 3 0.16030258696796906 1103 4 -0.2726889280658895 1103 5 -0.13765244905005147 1103 6 -0.1922285656567908 1103 7 -0.05173303320577105 1103 8 -0.10914512261152254 1103 9 -0.00896314672814924 1103 10-11 -0.2715771611752871 1103 12-13 -0.16697875670929818 1103 14-15 0.2233949835515716 1103 16-17 0.22787549486803726 1103 18-19 0.10661866702466938 1103 20-21 -0.10238594120165345 1103 22-23 -0.17633038353317687 1103 24-25 -0.07846271630193513 1103 26-27 -0.06959341287493004 1103 28-29 -0.18342680386250976 1103 30-31 0.014420928180946646 1103 32-33 -0.1317713023180147 1103 34-35 -0.19638249524553686 1103 36-37 -0.13009894462407345 1103 38-39 -0.08216571569084152 1103 40-41 0.0573696285753158 1103 42-43 0.22339498219960063 1103 44-45 0.13513973306406513 1103 46-47 0.10234045507102252 1103 48-49 0.1870674083395656 1103 50-51 -0.07045305316253092 1103 52-53 0.18679617380191615 1103 54-55 -0.01619089030089782 1103 56-57 -0.10068346783982207 1103 58-59 0.04380125461599249 1103 60-61 0.23527443684357507 1103 62-63 -0.018121545808639894 1103 64-65 0.005414876462985774 1103 66-67 0.005867324007162722 1103 68-69 -0.11839377449356903 1103 70-71 -0.0402996882952138 1103 72-73 -0.15314376964058596 1103 74-75 -0.29158479803481896 1103 76-77 -0.13670601160983864 1103 78-79 -0.10124783439569285 1103 80-81 -0.31731351282885356 1103 82-83 -0.3117767605067989 1103 84-85 -0.4713909370262144 1103 86-87 -0.43801151036728925 1103 88-89 -0.34046212269209164 1103 90-91 -0.35793567753099254 1103 92-93 -0.7870960232397692 1103 94-95 -0.21160723313721874 1103 96-97 -0.192510755242715 1103 98-99 -0.3729409438516136 1103 100-101 -0.32064265064350295 1104 1 0.2584886660903649 1104 2 0.32412526301532907 1104 3 0.33414491479932096 1104 4 0.531786984587967 1104 5 0.29930386738558923 1104 6 0.5959827482079945 1104 7 0.3602308908647842 1104 8 0.16121448242105885 1104 9 0.39166015467061044 1104 10-11 0.3617295891578678 1104 12-13 0.47365362138491207 1104 14-15 0.6244642497558388 1104 16-17 0.48989686969883905 1104 18-19 0.5805415308532531 1104 20-21 0.4336047302639443 1104 22-23 0.46439118752213204 1104 24-25 0.516408426102501 1104 26-27 0.47883443001953907 1104 28-29 0.5877893159288803 1104 30-31 0.7411310720439488 1104 32-33 0.4820559676428502 1104 34-35 0.5055411870596274 1104 36-37 0.5934946922451516 1104 38-39 0.37540991469352747 1104 40-41 0.5269626988069049 1104 42-43 0.5297832968723668 1104 44-45 0.7015465159264167 1104 46-47 0.7008252240612478 1104 48-49 0.7989363681397776 1104 50-51 0.8264766103176768 1104 52-53 0.8442348667577733 1104 54-55 0.8648611144420713 1104 56-57 0.6823467843139284 1104 58-59 0.782353075726391 1104 60-61 0.8066537272873333 1104 62-63 0.7446159960333532 1104 64-65 0.7367394444017847 1104 66-67 0.6297340487366938 1104 68-69 0.9178605408717502 1104 70-71 0.8797965351698807 1104 72-73 0.8622433278105959 1104 74-75 0.9544290636006636 1104 76-77 1.0197515587739048 1104 78-79 0.854755114218662 1104 80-81 0.7951666810871387 1104 82-83 0.8717403802816648 1104 84-85 0.8521486594018768 1104 86-87 0.7608323914574981 1104 88-89 0.9390397608533796 1104 90-91 0.9865537714663724 1104 92-93 0.8835996293903747 1104 94-95 1.0419368754053657 1104 96-97 0.8413419084788387 1104 98-99 0.8857540581945749 1104 100-101 0.7689897169574174 1105 1 0.029905863542591504 1105 2 0.22440392543571264 1105 3 0.2138523192579207 1105 4 0.34122169796376056 1105 5 0.24281501388239946 1105 6 0.30218298706149227 1105 7 0.3855095532851678 1105 8 0.42760580407711046 1105 9 0.28869439033939415 1105 10-11 0.3517226225973573 1105 12-13 0.34161241915560936 1105 14-15 0.566383039564748 1105 16-17 0.5132647040937357 1105 18-19 0.37399794008891973 1105 20-21 0.5060121745314632 1105 22-23 0.4592956461208644 1105 24-25 0.4587850184591886 1105 26-27 0.5221564841596589 1105 28-29 0.48321626656582595 1105 30-31 0.7104284446554132 1105 32-33 0.777786461273422 1105 34-35 0.8550435755946566 1105 36-37 0.5854333865126691 1105 38-39 0.6632433143750518 1105 40-41 0.8163984074056287 1105 42-43 0.7391383924137713 1105 44-45 0.8774919777098589 1105 46-47 1.1140218801122046 1105 48-49 1.1806590990314874 1105 50-51 1.2327813475788219 1105 52-53 1.270409229815094 1105 54-55 1.1980517991554436 1105 56-57 1.2462301441865336 1105 58-59 1.0669967859174747 1105 60-61 1.3525932177331939 1105 62-63 1.385559467370939 1105 64-65 1.2383367772043385 1105 66-67 1.2706377111570717 1105 68-69 1.2134019421456443 1105 70-71 1.2285674348514135 1105 72-73 1.012546870804222 1105 74-75 1.0700142546834641 1105 76-77 1.1276486527229466 1105 78-79 0.9928522479766286 1105 80-81 1.0570197861826856 1105 82-83 0.972651009262556 1105 84-85 1.0411116371088767 1105 86-87 1.0736210061071745 1105 88-89 0.8575956923820378 1105 90-91 0.7348618924854797 1105 92-93 0.7847043268426042 1105 94-95 0.8540139056601426 1105 96-97 0.6604700931922167 1105 98-99 0.677185188767826 1105 100-101 0.6287279733268427 1106 1 -0.026989416693396606 1106 2 0.07718784873954831 1106 3 0.1759144505263528 1106 4 0.23334927908470604 1106 5 0.09039982214198972 1106 6 -0.07923755128658883 1106 7 0.07199554148575515 1106 8 0.16004957251369234 1106 9 0.2703592133482431 1106 10-11 0.20892441094544267 1106 12-13 0.18219363228245555 1106 14-15 0.2989236000367228 1106 16-17 0.4039542321173357 1106 18-19 0.3303700824193001 1106 20-21 0.13678051892674148 1106 22-23 0.24093281426245738 1106 24-25 0.36240780606981104 1106 26-27 0.32216109330421006 1106 28-29 0.1344538218755602 1106 30-31 0.28728592990615454 1106 32-33 0.3513253624533661 1106 34-35 0.34072757264480913 1106 36-37 0.2734403924123754 1106 38-39 0.4776307590653204 1106 40-41 0.45652792436727907 1106 42-43 0.5818190708798454 1106 44-45 0.5329445957039596 1106 46-47 0.507154254743476 1106 48-49 0.5457563519813462 1106 50-51 0.46988265657586936 1106 52-53 0.4688144658032982 1106 54-55 0.3566386354386353 1106 56-57 0.22736491557297 1106 58-59 0.4714630469794159 1106 60-61 0.2888395304175546 1106 62-63 0.27524420188420606 1106 64-65 0.23380368354651893 1106 66-67 0.24378483505088155 1106 68-69 0.22431639642292822 1106 70-71 0.33599968042663164 1106 72-73 0.3164853848160192 1106 74-75 0.4821731779872991 1106 76-77 0.5047204631949214 1106 78-79 0.4068824839648215 1106 80-81 0.27467741892604636 1106 82-83 0.2326228934808512 1106 84-85 0.27751926985224173 1106 86-87 0.23160865359979965 1106 88-89 0.37006112379797074 1106 90-91 0.3528099013350392 1106 92-93 0.285805912388323 1106 94-95 0.40765282527666 1106 96-97 0.5071607274105006 1106 98-99 0.5758715825731322 1106 100-101 0.4978029180171113 1107 1 0.20570373588302004 1107 2 0.4340499974488061 1107 3 0.4762321269502259 1107 4 0.44127284362660646 1107 5 0.3528497929331422 1107 6 0.49976635449193907 1107 7 0.4186007971476897 1107 8 0.4122595479396338 1107 9 0.510354578555436 1107 10-11 0.43720364755644425 1107 12-13 0.39013047357459385 1107 14-15 0.505795887355255 1107 16-17 0.5500793522115757 1107 18-19 0.42522160007255394 1107 20-21 0.4105833181484826 1107 22-23 0.5489437639604731 1107 24-25 0.4454104276244877 1107 26-27 0.4990412018356025 1107 28-29 0.5437024060911924 1107 30-31 0.5589222048845457 1107 32-33 0.5630641822226892 1107 34-35 0.7371170207665116 1107 36-37 0.713885718754895 1107 38-39 0.6579893761590156 1107 40-41 0.5973990722992397 1107 42-43 0.6266486215463374 1107 44-45 0.6348134793465263 1107 46-47 0.5519463891138443 1107 48-49 0.67023433430154 1107 50-51 0.5928301916868364 1107 52-53 0.6729613983911946 1107 54-55 0.7652137263240277 1107 56-57 0.7349857702094553 1107 58-59 0.6886646459829322 1107 60-61 0.7989721048035676 1107 62-63 0.9482487063234686 1107 64-65 1.005032000199428 1107 66-67 0.6469828420899759 1107 68-69 0.7327145444369663 1107 70-71 0.7127033288775948 1107 72-73 0.6839423802150222 1107 74-75 0.6279060304608848 1107 76-77 0.6322146817736822 1107 78-79 0.5549737700715554 1107 80-81 0.5533551994396433 1107 82-83 0.572721334548973 1107 84-85 0.5066880487291741 1107 86-87 0.5230200445687174 1107 88-89 0.5819218482740212 1107 90-91 0.7217430299650225 1107 92-93 0.8052418677591326 1107 94-95 0.9178372996863295 1107 96-97 0.9900578591496583 1107 98-99 0.9751879506336216 1107 100-101 1.0267703730813444 1108 1 -0.01640095463922364 1108 2 -0.03980062001883411 1108 3 -0.02748290801480735 1108 4 0.18980124341830873 1108 5 0.1615650138824023 1108 6 0.37789321433422174 1108 7 0.29886182601244116 1108 8 0.27973648589529176 1108 9 0.13358075397576386 1108 10-11 0.11471978168826524 1108 12-13 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-0.4345545934010815 2316 78-79 -0.24857217062802306 2316 80-81 -0.31200879908863755 2316 82-83 -0.2008167717451954 2316 84-85 -0.37360493265856576 2316 86-87 -0.2407321915672398 2316 88-89 -0.28255086769548043 2316 90-91 -0.2432146579021186 2316 92-93 -0.37046766928142816 2316 94-95 -0.4912804257352086 2316 96-97 -0.34294560448220324 2316 98-99 -0.2403414585189978 2316 100-101 -0.23779940070416217 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14930.0 3 1843.0 4 1362.0 5 1162.0 6 1249.0 7 1276.0 8 1358.0 9 1405.0 10 1334.0 11 1458.0 12 1677.0 13 1973.0 14 2233.0 15 2735.0 16 3335.0 17 4187.0 18 3779.0 19 2329.0 20 2075.0 21 2040.0 22 2234.0 23 2347.0 24 2696.0 25 3046.0 26 3689.0 27 4352.0 28 5177.0 29 6396.0 30 7863.0 31 9920.0 32 12789.0 33 16919.0 34 23780.0 35 36231.0 36 58305.0 37 126247.0 38 248656.0 39 93696.0 40 107.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.36228957731395 23.737184993473967 15.810984928771433 21.089540500440645 2 14.490724536226812 24.12554890692742 41.38447958254486 19.999246974300906 3 16.86806965484929 30.08425180161085 33.223684210526315 19.82399433301354 4 12.023906971425703 20.956390415457534 41.62579677122016 25.393905841896604 5 11.722745550777743 39.70557888689969 36.10590571162459 12.465769850697988 6 24.65232277383415 40.468290833254336 21.5465084507592 13.332877942152308 7 22.61630411597161 35.65572113501162 26.4088311521737 15.319143596843068 8 22.685104514438255 35.464233688568584 24.36277208952207 17.48788970747109 9 25.495722671305725 17.9622714099921 24.258036457309654 32.28396946139252 10-11 21.36925050751569 28.928917139569776 31.507368646994177 18.194463705920356 12-13 22.772138514222306 28.311620058228893 30.423236352560114 18.49300507498869 14-15 21.337657548122806 29.657285992848593 28.460830045670566 20.54422641335803 16-17 19.08570081930923 32.053042097755146 29.575870991042745 19.285386091892875 18-19 18.776576793905264 30.85093848023443 31.772441638249525 18.60004308761078 20-21 21.0865971892531 30.03709505494544 30.82368311160655 18.052624644194914 22-23 20.82661796280289 29.887976465995596 30.423207762875386 18.86219780832613 24-25 19.905196093259388 30.460965003683565 30.922647901970446 18.7111910010866 26-27 19.59910447428291 30.27989360753 31.2465149217112 18.874486996475888 28-29 19.502334419336716 30.037752209995972 31.416098222662757 19.043815148004555 30-31 20.1318832357324 30.117776287781737 30.651456626013935 19.09888385047193 32-33 19.741201823825993 30.16198931408586 30.91806025428182 19.17874860780632 34-35 19.936439954299225 30.598188001943466 30.58870767049435 18.876664373262955 36-37 19.933524246640662 29.8542775641544 30.689337389544015 19.52286079966093 38-39 19.802774511519107 30.205136533484634 31.01338743976976 18.978701515226497 40-41 19.529537392841434 30.006071069853547 31.132320744866597 19.332070792438415 42-43 19.705924992472482 29.864727726912825 30.592736224643417 19.83661105597127 44-45 19.901080515859185 30.07138375740676 30.525758103868945 19.50177762286511 46-47 19.921981469902008 29.923786938030617 30.252326370081928 19.901905221985444 48-49 19.526255942541663 30.063491687239125 30.365159119635855 20.04509325058336 50-51 19.531190093452473 29.805176065125345 30.310848322024135 20.352785519398044 52-53 19.84543411712882 30.055731818156474 30.44737399702243 19.651460067692273 54-55 20.14638382334698 29.831035544980754 29.824623760678758 20.197956870993504 56-57 19.953770312621987 29.793262957138555 29.842964053490366 20.41000267674909 58-59 19.457621637144022 29.94149372908125 30.24315579786411 20.35772883591061 60-61 20.22293748327237 30.10924536087073 29.83972142920867 19.82809572664823 62-63 19.761711927088925 30.015807808276655 30.367789429957053 19.85469083467737 64-65 19.70883065873874 30.00872127677819 29.944305131749836 20.33814293273323 66-67 19.94762054802542 30.072037431022803 29.84534328965823 20.134998731293553 68-69 19.732611672062394 29.84653544031655 30.036431624825372 20.384421262795684 70-71 19.846934508043606 29.62116987760337 30.319444057211363 20.212451557141662 72-73 19.90917613765078 29.46708070637684 29.981772494240737 20.641970661731648 74-75 19.78895810872121 29.56231213598898 29.64573368802012 21.002996067269688 76-77 19.523397907328533 30.06824534865078 30.25855158135417 20.149805162666517 78-79 19.859215017064848 29.62458732070758 29.828975105400524 20.687222556827052 80-81 19.894137920457748 29.54783103335778 29.66682653564846 20.891204510536017 82-83 19.9575433716419 30.028269561246653 29.76125989521788 20.25292717189357 84-85 19.94285292050993 29.356018358447006 29.79302133639679 20.90810738464627 86-87 19.34786730915094 29.64288303663053 30.313322625262124 20.695927028956408 88-89 20.18749333443003 29.09799241745672 29.847115350432972 20.86739889768028 90-91 19.811964584595373 29.41728818828916 30.215326831260974 20.555420395854494 92-93 20.25777321646201 29.463760315251374 29.84645997584858 20.432006492438042 94-95 20.04827735711094 28.74621765796963 30.13445306689474 21.071051918024686 96-97 20.11208265206986 28.734404119222155 30.08076195573843 21.072751272969555 98-99 19.92787789702876 29.15418225258209 29.869130582963766 21.048809267425383 100-101 19.769438021738342 29.121471424148694 30.088944795097856 21.020145759015108 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1812.0 1 1051.0 2 269.5 3 232.5 4 191.0 5 186.0 6 239.0 7 391.5 8 590.0 9 619.5 10 467.0 11 362.5 12 358.0 13 341.0 14 340.5 15 361.5 16 384.5 17 426.5 18 474.5 19 525.0 20 588.5 21 666.5 22 742.5 23 861.0 24 990.5 25 1161.0 26 1530.0 27 2325.5 28 3560.0 29 5267.0 30 8507.5 31 13292.0 32 20700.5 33 29823.5 34 39207.0 35 46395.0 36 51915.5 37 55921.0 38 52413.0 39 45985.5 40 39660.5 41 32713.0 42 27486.5 43 24022.5 44 21811.5 45 19624.5 46 17621.5 47 16080.5 48 15020.5 49 13888.5 50 12509.5 51 11307.0 52 10134.5 53 9026.5 54 8102.5 55 7292.0 56 6420.5 57 5692.5 58 5070.5 59 4377.5 60 3632.0 61 3050.5 62 2528.0 63 1954.5 64 1537.0 65 1224.0 66 1005.0 67 849.0 68 689.0 69 565.0 70 470.5 71 389.0 72 359.5 73 308.0 74 236.5 75 195.5 76 164.0 77 142.5 78 115.0 79 90.0 80 62.5 81 47.5 82 39.0 83 30.5 84 23.0 85 15.0 86 10.5 87 7.0 88 5.5 89 4.0 90 2.0 91 2.0 92 2.0 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14787173310683802 2 0.15079575042815968 3 0.14675782174633453 4 0.14996031690778208 5 0.13728957518205487 6 0.11946699341399908 7 0.12684665617733468 8 0.1148721090519222 9 0.1118088528105376 10-11 0.10087859758559713 12-13 0.11814422367340119 14-15 0.13763767248221223 16-17 0.14780211364680657 18-19 0.14571352984586253 20-21 0.1362452832815829 22-23 0.12949219565853048 24-25 0.11341010039126137 26-27 0.11779612637324384 28-29 0.1226694885754466 30-31 0.11320124201116696 32-33 0.11097341929015998 34-35 0.1274732313176179 36-37 0.13032762917890808 38-39 0.11675183447277183 40-41 0.11884041827371586 42-43 0.11445439229173339 44-45 0.13088458485915982 46-47 0.1287263815981843 48-49 0.14056168980353387 50-51 0.12907447889834167 52-53 0.11501134797198512 54-55 0.10596081816789428 56-57 0.12559350589676826 58-59 0.11570754257229982 60-61 0.1133404809312299 62-63 0.11459363121179632 64-65 0.1362452832815829 66-67 0.12865676213815286 68-69 0.1331124075801668 70-71 0.11835308205349558 72-73 0.12078976315459697 74-75 0.10435957058717053 76-77 0.1287960010582158 78-79 0.12893523997827872 80-81 0.13032762917890808 82-83 0.1374984335621493 84-85 0.12782132861777523 86-87 0.13561870814129967 88-89 0.12315682479566688 90-91 0.12970105403862486 92-93 0.1459223882259569 94-95 0.12204291343516341 96-97 0.10763168520864952 98-99 0.11076456091006558 100-101 0.15539063479023657 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 718190.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.09480916648225 #Duplication Level Percentage of deduplicated Percentage of total 1 66.96999035809382 26.18178992930834 2 15.284471306971897 11.950869779132796 3 6.61049003873616 7.753075396839659 4 3.3609924080911666 5.2558942721727915 5 1.895045728880531 3.704322556617081 6 1.2149347534137693 2.849858540066309 7 0.8284330198668785 2.2671201573235744 8 0.5899895593201193 1.845242338546962 9 0.45829985149031577 1.6125430711536943 >10 2.4007794042727357 17.302007138401574 >50 0.228626540253869 6.2410288255521875 >100 0.15001361811239278 10.619574697582001 >500 0.006851583519556241 1.7993233749550264 >1k 0.001081828976772038 0.6173499223480421 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1816 0.2528578788342917 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1469 0.20454197357245296 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1094 0.15232737854885198 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 960 0.13366936326041856 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 943 0.13130230161934864 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 849 0.11821384313343267 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 785 0.10930255224940474 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 765 0.10651777384814602 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 723 0.10066973920550273 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.39238920062936E-4 0.0 18-19 0.0 0.0 0.0 2.08858380094404E-4 0.0 20-21 0.0 0.0 0.0 2.78477840125872E-4 0.0 22-23 0.0 0.0 0.0 0.0011835308205349559 0.0 24-25 0.0 0.0 0.0 0.0027847784012587197 0.0 26-27 0.0 0.0 0.0 0.005708795722580375 0.0 28-29 0.0 0.0 0.0 0.010582157924783136 0.0 30-31 0.0 0.0 0.0 0.02422757209095086 0.0 32-33 0.0 0.0 0.0 0.05458165666467091 0.0 34-35 0.0 0.0 0.0 0.10428995112713906 0.0 36-37 0.0 0.0 0.0 0.1757891365794567 0.0 38-39 0.0 0.0 0.0 0.2503515782731589 0.0 40-41 0.0 0.0 0.0 0.33201520489007086 0.0 42-43 0.0 0.0 0.0 0.4238432726715772 0.0 44-45 0.0 0.0 0.0 0.5504810704688174 0.0 46-47 0.0 0.0 0.0 0.6904858045920996 0.0 48-49 0.0 0.0 0.0 0.8212311505311964 0.0 50-51 0.0 0.0 0.0 0.9443879753268634 0.0 52-53 0.0 0.0 0.0 1.0816775505089182 0.0 54-55 0.0 0.0 0.0 1.2372770436792493 0.0 56-57 0.0 0.0 0.0 1.4166167727203107 0.0 58-59 0.0 0.0 0.0 1.5807794594745124 0.0 60-61 0.0 0.0 0.0 1.7501636057310739 0.0 62-63 0.0 0.0 0.0 1.9339589802141495 0.0 64-65 0.0 0.0 0.0 2.1689942772803854 0.0 66-67 0.0 0.0 0.0 2.43953549896267 0.0 68-69 0.0 0.0 0.0 2.7140450298667482 0.0 70-71 0.0 0.0 0.0 2.997396232194823 0.0 72-73 0.0 0.0 0.0 3.288753672426517 0.0 74-75 0.0 1.39238920062936E-4 0.0 3.6147816037538814 0.0 76-77 0.0 1.39238920062936E-4 0.0 3.993372227405004 0.0 78-79 0.0 1.39238920062936E-4 0.0 4.381013380860218 0.0 80-81 0.0 1.39238920062936E-4 0.0 4.779863267380499 0.0 82-83 0.0 1.39238920062936E-4 0.0 5.187624444784806 0.0 84-85 0.0 1.39238920062936E-4 0.0 5.6304738300449735 0.0 86-87 0.0 1.39238920062936E-4 0.0 6.124354279508208 0.0 88-89 0.0 1.39238920062936E-4 0.0 6.675461925117309 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1720 0.0 52.20297 1 GCGTCAG 135 0.0 45.747925 1 CGGTCTA 30 8.29092E-5 39.58403 28-29 GTATCAA 2915 0.0 39.44016 1 CGTCAGA 170 0.0 36.329235 2 GTGGTAT 905 0.0 30.971716 1 ATCAACG 3700 0.0 30.687254 3 TCAACGC 3770 0.0 30.117464 4 TACGTCG 40 4.4955616E-4 29.685955 22-23 TATCAAC 3825 0.0 29.6844 2 CAACGCA 3865 0.0 29.373093 5 AACGCAG 4040 0.0 28.331945 6 ACGCAGA 4640 0.0 24.670052 7 CGCAGAG 4665 0.0 24.541264 8 CGCTGTA 60 1.7100907E-4 23.752073 16-17 ACGATCT 50 0.001655018 23.747107 74-75 GACGATC 50 0.001655018 23.747107 72-73 GTACATG 4375 0.0 23.346523 1 GCAGAGT 5030 0.0 22.288229 9 CATTCCG 195 4.2133252E-7 21.924992 9 >>END_MODULE