FastQCFastQC Report
Fri 10 Feb 2017
SRR2031750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718190
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT90561.2609476600899483No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT40240.5602974143332544RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT24240.33751514223255685RNA PCR Primer, Index 46 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22650.31537615394255003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21660.30159150085631936No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG16600.23113660730447375RNA PCR Primer, Index 46 (96% over 28bp)
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC10820.15065651150809675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10290.14327684874476115No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA9630.13408708002060735No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC9250.1287960010582158No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT9040.12587198373689412RNA PCR Primer, Index 46 (96% over 26bp)
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC8730.12155557721494313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8500.11835308205349558No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG8080.1125050474108523No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.11153037497041174No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC7610.10596081816789428No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18800.052.8290061
GTATCAA32450.038.0752371
TTCGATA902.4102255E-531.6695234
CCGTGCT600.003950922731.6651139
CATTCCG1201.505141E-731.6651139
TCAACGC40000.030.52154
ATCAACG40450.030.1819533
CAACGCA41100.029.7004895
TATCAAC42300.028.861942
AACGCAG42950.028.6423636
CGGAGAT1354.2052307E-728.160491
GTGGTAT9700.027.924611
TCTCGCA350.00839426727.14152338-39
CTCGCAT350.00839426727.14152338-39
CGCAGAG48450.025.0968078
ACGCAGA48900.024.9629887
GTGTCGC500.001650300123.7587630-31
CTTCGAT1000.00165217623.7521423
ATAGTGC1201.7105145E-423.7488356
CTATAAC5100.023.2864133
Produced by FastQC (version 0.11.5)