##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031750_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 718190 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65326863364848 34.0 31.0 34.0 31.0 34.0 2 32.82748437043122 34.0 31.0 34.0 31.0 34.0 3 32.877688355449116 34.0 31.0 34.0 31.0 34.0 4 36.30626296662443 37.0 37.0 37.0 35.0 37.0 5 36.275180662498784 37.0 37.0 37.0 35.0 37.0 6 36.30825965273813 37.0 37.0 37.0 35.0 37.0 7 36.25341344212534 37.0 37.0 37.0 35.0 37.0 8 36.246521115582226 37.0 37.0 37.0 35.0 37.0 9 38.07684456759353 39.0 39.0 39.0 35.0 39.0 10-11 38.0384870298946 39.0 39.0 39.0 35.0 39.0 12-13 37.99083529428145 39.0 39.0 39.0 35.0 39.0 14-15 39.418162324733004 41.0 39.0 41.0 36.0 41.0 16-17 39.381493755134436 41.0 39.0 41.0 36.0 41.0 18-19 39.43175691669335 41.0 39.0 41.0 36.0 41.0 20-21 39.46654297609268 41.0 39.0 41.0 36.0 41.0 22-23 39.369586042690656 41.0 39.0 41.0 36.0 41.0 24-25 39.29009454322672 41.0 39.0 41.0 36.0 41.0 26-27 39.16937300714296 41.0 39.0 41.0 36.0 41.0 28-29 39.06464445341762 40.5 39.0 41.0 35.5 41.0 30-31 38.88682590957825 40.0 38.5 41.0 35.0 41.0 32-33 38.65452317631824 40.0 38.0 41.0 35.0 41.0 34-35 38.48349949177794 40.0 38.0 41.0 34.5 41.0 36-37 38.361015190966185 40.0 38.0 41.0 34.0 41.0 38-39 38.35253275595595 40.0 38.0 41.0 34.0 41.0 40-41 38.302545983653346 40.0 38.0 41.0 34.0 41.0 42-43 38.18957935922249 40.0 38.0 41.0 34.0 41.0 44-45 37.993271975382555 40.0 38.0 41.0 33.0 41.0 46-47 37.76257188209249 40.0 38.0 41.0 33.0 41.0 48-49 37.55836477812278 40.0 38.0 41.0 33.0 41.0 50-51 37.336284966373796 40.0 38.0 41.0 32.5 41.0 52-53 37.47312967320626 40.0 38.0 41.0 33.0 41.0 54-55 37.441956863782565 40.0 38.0 41.0 33.0 41.0 56-57 37.23756666063298 40.0 37.0 41.0 32.0 41.0 58-59 37.06648588813545 40.0 37.0 41.0 32.0 41.0 60-61 36.791518261184365 40.0 37.0 41.0 31.0 41.0 62-63 36.49878653281165 40.0 36.0 41.0 31.0 41.0 64-65 36.119660535512885 39.0 36.0 41.0 30.5 41.0 66-67 35.668964340912574 39.0 35.5 41.0 29.0 41.0 68-69 35.13668736685278 39.0 35.0 41.0 28.0 41.0 70-71 34.77705272977903 38.0 35.0 40.0 27.0 41.0 72-73 34.40239073225748 37.0 35.0 39.5 26.0 41.0 74-75 33.99473328784862 37.0 35.0 39.0 26.0 41.0 76-77 33.03005193611718 36.0 33.5 39.0 25.5 39.5 78-79 33.203013826424765 36.0 34.0 37.5 26.0 39.5 80-81 32.917395118283466 35.5 34.0 37.0 26.0 39.0 82-83 32.5876063437252 35.0 34.0 37.0 25.5 39.0 84-85 32.218156058981606 35.0 34.0 36.0 25.0 37.5 86-87 31.92729430930534 35.0 34.0 36.0 24.0 37.0 88-89 31.671779055681647 35.0 34.0 36.0 23.0 37.0 90-91 31.440682827663988 35.0 34.0 35.0 21.0 36.0 92-93 31.242732424567315 35.0 33.5 35.0 20.0 36.0 94-95 31.0404844122029 35.0 33.0 35.0 18.5 36.0 96-97 30.854659630459906 35.0 33.0 35.0 11.0 36.0 98-99 30.66183739678915 35.0 33.0 35.0 2.0 35.5 100-101 30.0139343349253 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.02114550009980576 1101 2 -0.00793458550539583 1101 3 0.07900228248970365 1101 4 0.049271816923983636 1101 5 0.037530431143402154 1101 6 0.030226048018299423 1101 7 0.08983887045597072 1101 8 -0.02224803297196587 1101 9 -0.1368074323916062 1101 10-11 -0.0806685610860356 1101 12-13 0.01461354782153279 1101 14-15 0.04141906197143186 1101 16-17 0.08302600899248347 1101 18-19 0.029820573937250572 1101 20-21 0.031148153775987453 1101 22-23 -0.2406863230026417 1101 24-25 -0.1336934580624458 1101 26-27 0.014294097860677368 1101 28-29 0.008919686190381526 1101 30-31 0.08698208556157283 1101 32-33 0.23551808983010147 1101 34-35 -0.19384095386853062 1101 36-37 0.10048827893119494 1101 38-39 0.12374932631087177 1101 40-41 -0.2125548514587976 1101 42-43 -0.32808137230830425 1101 44-45 -0.3463535616657296 1101 46-47 -0.38091466065827717 1101 48-49 -0.5882274482579675 1101 50-51 -0.3462466845897936 1101 52-53 -0.46982232249775535 1101 54-55 -0.48976207855373133 1101 56-57 -0.7358333896399074 1101 58-59 -0.21018145956857381 1101 60-61 -0.3931709214801984 1101 62-63 -0.27947755354682613 1101 64-65 -0.2560729943480027 1101 66-67 -0.471548943901098 1101 68-69 -0.19113988888850741 1101 70-71 -0.23236011911729548 1101 72-73 -0.4204412715843304 1101 74-75 -0.35234577590367167 1101 76-77 -0.3317526577038521 1101 78-79 -0.227896845323599 1101 80-81 -0.21656004871439194 1101 82-83 -0.12038792788161601 1101 84-85 0.10869324202003128 1101 86-87 0.06640246943655015 1101 88-89 -0.06802206127076715 1101 90-91 -0.10714443827508902 1101 92-93 -0.23242733931273918 1101 94-95 -0.07358746927677373 1101 96-97 -0.13493626151657168 1101 98-99 -0.5635509893454298 1101 100-101 -0.7377503839355626 1102 1 0.2128612339069278 1102 2 0.10297846658412624 1102 3 0.08811299748277435 1102 4 0.016988196405073097 1102 5 0.08971898333194872 1102 6 0.13311770973349013 1102 7 0.1501478425296483 1102 8 0.19432950713498798 1102 9 0.10918187242122457 1102 10-11 0.2879687928454402 1102 12-13 0.1546907908399504 1102 14-15 0.14594471061472092 1102 16-17 0.09099788461141145 1102 18-19 0.15667737726464992 1102 20-21 0.15770786857099495 1102 22-23 0.1891512685996588 1102 24-25 0.2321710695667818 1102 26-27 0.17650443654159886 1102 28-29 0.22460596070018113 1102 30-31 0.10510448603691458 1102 32-33 0.20803740058470055 1102 34-35 0.210942171502829 1102 36-37 0.21174793430849093 1102 38-39 0.08468218430255092 1102 40-41 0.1504378203574035 1102 42-43 0.03382197488916461 1102 44-45 0.04629844863334398 1102 46-47 -0.015693824849208227 1102 48-49 0.1170607276184441 1102 50-51 -0.016032780846479966 1102 52-53 0.09098179712989207 1102 54-55 0.27439914148984457 1102 56-57 0.25188695102161063 1102 58-59 0.1733103209820328 1102 60-61 0.2155634813718521 1102 62-63 0.10480646309012798 1102 64-65 0.2999777087615243 1102 66-67 0.24879171761602947 1102 68-69 0.004096791642290043 1102 70-71 0.11765968678486871 1102 72-73 0.1671008159577596 1102 74-75 0.041841191812707734 1102 76-77 0.09065772058887234 1102 78-79 -0.13223433789638506 1102 80-81 -0.37703737095642254 1102 82-83 -0.11424809821237858 1102 84-85 -0.034899548631578625 1102 86-87 -0.10382925961870981 1102 88-89 -0.22305276041911526 1102 90-91 -0.27945578705702445 1102 92-93 -0.12537643814419042 1102 94-95 -0.1805393409345264 1102 96-97 -0.26179306484396037 1102 98-99 -0.547755782373752 1102 100-101 -0.6136168921550116 1103 1 0.1721548843043692 1103 2 0.029636590935830043 1103 3 0.19362168124516188 1103 4 0.15804733252186765 1103 5 0.05510319336195835 1103 6 0.1363820943817302 1103 7 0.16401801043605957 1103 8 0.06565395424197362 1103 9 0.12935061087662092 1103 10-11 0.21612875601363868 1103 12-13 0.06765486319446978 1103 14-15 0.10669582416650059 1103 16-17 0.1326652446478107 1103 18-19 0.2197629279943456 1103 20-21 0.17885009811520547 1103 22-23 0.18779365068520093 1103 24-25 0.02560374891380235 1103 26-27 0.13676603107008134 1103 28-29 0.2474455593223226 1103 30-31 0.15151588170696328 1103 32-33 0.05682594500751037 1103 34-35 -0.13192222202054182 1103 36-37 -0.26157258555238627 1103 38-39 -0.3350380826170536 1103 40-41 -0.20911459562005064 1103 42-43 -0.2575084074626375 1103 44-45 -0.16931179992583623 1103 46-47 -0.15505004493029872 1103 48-49 -0.22674803546544098 1103 50-51 -0.014273008493624673 1103 52-53 -0.15142025746326482 1103 54-55 -0.13676216958909748 1103 56-57 -0.10750876339881899 1103 58-59 -0.05237458962874797 1103 60-61 -0.47783674827220324 1103 62-63 -0.5224421349162895 1103 64-65 -0.23071083510658497 1103 66-67 -0.10765856222798931 1103 68-69 -0.33718117195856934 1103 70-71 -0.2182099929892658 1103 72-73 -0.1461414363289748 1103 74-75 -0.1845675967871827 1103 76-77 -0.22973872048302013 1103 78-79 -0.17558381287683034 1103 80-81 -0.34458073429879477 1103 82-83 -0.34954449990086545 1103 84-85 -0.45513429588445575 1103 86-87 -0.4511658878449616 1103 88-89 -0.41201681058580064 1103 90-91 -0.36979908827347074 1103 92-93 -0.133377924728876 1103 94-95 -0.29591051561377313 1103 96-97 -0.32168356789756203 1103 98-99 -0.21206637872717948 1103 100-101 -0.4132372028955871 1104 1 0.16250630457111015 1104 2 0.17027028069122707 1104 3 0.12014242123194663 1104 4 0.022644547602823195 1104 5 0.13572576023045002 1104 6 0.13671525632467052 1104 7 0.12372115527493577 1104 8 0.14819941641891177 1104 9 0.15024537367580848 1104 10-11 0.2090693954747067 1104 12-13 0.2548564602731318 1104 14-15 0.3561947695908998 1104 16-17 0.2851957014207045 1104 18-19 0.3533897236585233 1104 20-21 0.3644488452164367 1104 22-23 0.22503827025098389 1104 24-25 0.28261982463337887 1104 26-27 0.31839306807991363 1104 28-29 0.293366817677601 1104 30-31 0.40946979517929094 1104 32-33 0.34605773128019024 1104 34-35 0.3102151561261266 1104 36-37 0.33002465051514207 1104 38-39 0.2814990793678547 1104 40-41 0.46119936360302205 1104 42-43 0.29619624840994163 1104 44-45 0.2783214386181143 1104 46-47 0.342766039183914 1104 48-49 0.39008556194900734 1104 50-51 0.35245303270302486 1104 52-53 0.5313059516260168 1104 54-55 0.6748098301170913 1104 56-57 0.5139877895555713 1104 58-59 0.5098873438647828 1104 60-61 0.7163114064828378 1104 62-63 0.44867508562117564 1104 64-65 0.5610813138141779 1104 66-67 0.7307342855458643 1104 68-69 0.7307877993842524 1104 70-71 0.5626402782622719 1104 72-73 0.5628389831927407 1104 74-75 0.42968727472248247 1104 76-77 0.5322498628179559 1104 78-79 0.5334753063164399 1104 80-81 0.6682027156849486 1104 82-83 0.6484237369116883 1104 84-85 0.7616701826515211 1104 86-87 0.751531674563882 1104 88-89 0.7221530128121358 1104 90-91 0.8203721097453887 1104 92-93 0.74078785875259 1104 94-95 0.8356254921092869 1104 96-97 0.7012127898816374 1104 98-99 0.752827009451078 1104 100-101 0.8318676775333813 1105 1 0.13875033323353136 1105 2 0.12907800362116717 1105 3 0.03366750084978065 1105 4 -0.016587140295264646 1105 5 0.08700959781006645 1105 6 0.07171724677052538 1105 7 -0.007270086763277561 1105 8 0.12463253743801772 1105 9 -0.015439339699987897 1105 10-11 0.11015418846833569 1105 12-13 0.06992911154702597 1105 14-15 0.008155358762870435 1105 16-17 0.03750461861815069 1105 18-19 0.05192674595151203 1105 20-21 0.1035412019043278 1105 22-23 0.20448691674780406 1105 24-25 0.07805175138496878 1105 26-27 0.2685025426022136 1105 28-29 0.2767366425183653 1105 30-31 0.07438321078438292 1105 32-33 0.029596744019052323 1105 34-35 -0.03755056839616344 1105 36-37 0.19864029541960804 1105 38-39 0.08907770197932052 1105 40-41 0.20026683971767056 1105 42-43 0.005212968155163367 1105 44-45 0.16333338129327757 1105 46-47 0.2191493990565192 1105 48-49 0.2724900205477354 1105 50-51 0.3610268782444237 1105 52-53 0.18078345958780062 1105 54-55 0.01130465495786126 1105 56-57 0.04322743923709993 1105 58-59 0.19932305246351945 1105 60-61 0.24463545106882378 1105 62-63 0.3022180792517446 1105 64-65 0.3254722373810566 1105 66-67 0.2507382664375797 1105 68-69 0.19686562581737377 1105 70-71 0.006703773485199349 1105 72-73 0.11237122841567526 1105 74-75 0.22654237026388557 1105 76-77 0.08908206823196707 1105 78-79 0.42847431109350964 1105 80-81 0.3255773175957728 1105 82-83 0.31875116525563385 1105 84-85 0.3703535027152185 1105 86-87 0.28471240704795164 1105 88-89 0.31417630102869865 1105 90-91 0.35803632152883225 1105 92-93 0.40305895747870224 1105 94-95 0.36238206058062516 1105 96-97 0.29208161949947353 1105 98-99 0.36103262250840373 1105 100-101 0.37821023326586456 1106 1 0.08357426391199851 1106 2 0.05741963341467482 1106 3 0.11655374716246314 1106 4 0.10801647327405561 1106 5 0.059654324948709814 1106 6 -0.005361477418261984 1106 7 -0.015133287353251035 1106 8 0.04174168198079542 1106 9 0.14632319717316733 1106 10-11 0.08403416634444483 1106 12-13 0.06536836302194615 1106 14-15 0.12447633958883131 1106 16-17 0.2047981289426346 1106 18-19 0.3491105586358785 1106 20-21 0.3376488715208481 1106 22-23 0.30042626040562226 1106 24-25 0.39039043132597584 1106 26-27 0.21766828743997024 1106 28-29 0.297279599745508 1106 30-31 0.2730673000174235 1106 32-33 0.312533690921704 1106 34-35 0.32871279812301424 1106 36-37 0.39955521061134647 1106 38-39 0.21519081038639598 1106 40-41 0.4507715410451141 1106 42-43 0.3301853132878989 1106 44-45 0.22084628689799501 1106 46-47 0.17723844772908848 1106 48-49 0.10394282290761936 1106 50-51 0.09438556187274827 1106 52-53 0.06686153849635446 1106 54-55 -0.06316529341972199 1106 56-57 0.049099489384595074 1106 58-59 -0.07382176685802477 1106 60-61 -0.2004327642704098 1106 62-63 -0.11310271720843446 1106 64-65 0.04836124918046636 1106 66-67 -0.0010592999340985898 1106 68-69 0.041811698826229815 1106 70-71 0.003770052983725236 1106 72-73 0.05448498210298425 1106 74-75 0.021914789142940094 1106 76-77 -0.07202366554383843 1106 78-79 -0.029704615897635733 1106 80-81 0.007680009336183957 1106 82-83 -0.07718126468537179 1106 84-85 -0.0071515673437758664 1106 86-87 0.04956307076476563 1106 88-89 0.07880803037972939 1106 90-91 0.05553125146983717 1106 92-93 0.10579218019256587 1106 94-95 0.19295221176056643 1106 96-97 0.18434977952896858 1106 98-99 0.16981073829011706 1106 100-101 0.1836328918204373 1107 1 0.11742514910914537 1107 2 0.09746231213180323 1107 3 -0.02799626756266349 1107 4 0.0027101182972018023 1107 5 -0.1124968465434506 1107 6 -0.02215294553716518 1107 7 0.07437373484064835 1107 8 0.06178474693065539 1107 9 -0.027652923988036093 1107 10-11 0.07516288323101605 1107 12-13 0.11124074207075552 1107 14-15 0.11283262963030438 1107 16-17 0.1903892340027653 1107 18-19 0.24222185642614846 1107 20-21 0.21526378782904487 1107 22-23 0.19450072607677527 1107 24-25 -0.10442318724023636 1107 26-27 -0.022672273273407484 1107 28-29 -0.028927739642021777 1107 30-31 0.14040842559617062 1107 32-33 0.11898401897813216 1107 34-35 0.300830415646395 1107 36-37 0.18578866898752722 1107 38-39 0.2725717179367635 1107 40-41 0.36292411263093527 1107 42-43 0.31636783722225914 1107 44-45 0.3030173010968795 1107 46-47 -0.0020990665735922676 1107 48-49 -0.049911779779591825 1107 50-51 0.055496753449006064 1107 52-53 0.14910255737830624 1107 54-55 0.11888188490875962 1107 56-57 0.23110080257588805 1107 58-59 -0.06492996513059524 1107 60-61 0.07642248052872702 1107 62-63 0.20337397123211076 1107 64-65 0.28522571528612417 1107 66-67 0.4899209587452731 1107 68-69 0.5011196663247404 1107 70-71 0.5292372738943669 1107 72-73 0.44207918668080737 1107 74-75 0.4874773129807437 1107 76-77 0.44239247125979375 1107 78-79 0.3649552337612718 1107 80-81 0.7112693184141037 1107 82-83 0.6823623047810017 1107 84-85 0.7124374327479543 1107 86-87 0.6217572106486138 1107 88-89 0.6108991426817312 1107 90-91 0.37700241195436845 1107 92-93 0.4756474394754271 1107 94-95 0.5929070708097584 1107 96-97 0.8010282745731239 1107 98-99 0.7482884019683063 1107 100-101 0.9201509554426224 1108 1 -0.018778075857376564 1108 2 -0.03754131456065579 1108 3 -0.07613363551385532 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-0.9519866470013412 2316 54-55 -0.9432374962049366 2316 56-57 -0.8815060878946852 2316 58-59 -0.9469523835830032 2316 60-61 -0.9407085411792409 2316 62-63 -0.8744776571823607 2316 64-65 -0.8288640029290235 2316 66-67 -0.5618828575934316 2316 68-69 -0.6680265737950251 2316 70-71 -0.628004317600066 2316 72-73 -0.7663715041424695 2316 74-75 -0.7060642188834052 2316 76-77 -1.132435615876556 2316 78-79 -0.9372173071235395 2316 80-81 -0.7475937095360123 2316 82-83 -0.6753924878451407 2316 84-85 -0.7102969333312927 2316 86-87 -0.6469494146574739 2316 88-89 -0.7274964218395183 2316 90-91 -0.7362769052153517 2316 92-93 -0.6658000541492051 2316 94-95 -0.6486947320550343 2316 96-97 -0.6326821886400644 2316 98-99 -0.5576023096621405 2316 100-101 -0.3535060942535182 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 1.0 5 5.0 6 19.0 7 53.0 8 157.0 9 342.0 10 559.0 11 941.0 12 1397.0 13 1836.0 14 2184.0 15 2783.0 16 3757.0 17 5024.0 18 4905.0 19 3411.0 20 2993.0 21 3554.0 22 4420.0 23 4861.0 24 5708.0 25 6100.0 26 4446.0 27 3831.0 28 4824.0 29 5868.0 30 7660.0 31 9715.0 32 12752.0 33 16557.0 34 22595.0 35 34109.0 36 55302.0 37 122784.0 38 261351.0 39 101249.0 40 132.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.44159456075628 24.354737403574447 16.279989582767556 21.923678452901715 2 14.075383951322074 23.67298347234019 42.419554713933636 19.8320778624041 3 15.804071948336215 30.58361251047785 34.377497625949964 19.234817915235972 4 11.201742499467308 22.05225548674255 40.87339443408458 25.872607579705566 5 10.75272560186023 40.152327378548854 36.92922485693201 12.165722162658907 6 23.749564878374805 41.47342625210599 21.04429190047202 13.732716969047187 7 21.262897004970828 35.90261629930798 25.99298235842883 16.84150433729236 8 21.899218869658448 36.32506718277893 24.80318578649104 16.972528161071583 9 24.299280134783274 17.675406229549285 23.688995948147426 34.336317687520015 10-11 20.76568927838683 28.93449430825708 31.860950348062733 18.438866065293354 12-13 22.28727773987385 27.884055751263592 30.555702529971178 19.27296397889138 14-15 20.698506452684146 29.334806482133196 28.110756283682786 21.85593078149987 16-17 19.04601777551279 32.41148794381345 29.12296238124479 19.41953189942897 18-19 18.641319596011915 30.472319631517863 31.670332133789202 19.216028638681017 20-21 20.27360447792367 29.59989696319915 30.81419958506802 19.31229897380916 22-23 20.193402859967417 30.456912516186524 30.11097341929016 19.238711204555898 24-25 19.79671117670811 30.721953800526325 31.043177989111513 18.438157033654047 26-27 19.460963618211917 30.841811835046208 31.582563921196233 18.114660625545643 28-29 18.83348221030433 29.49226106638613 32.475880886355235 19.198375836954305 30-31 19.62416552200432 30.739005149762217 30.740327922265532 18.896501405967932 32-33 20.536364484458783 29.735500642590303 31.174958611058123 18.553176261892794 34-35 19.668294788732883 29.93785749348113 31.370581702600237 19.02326601518575 36-37 19.22450590848994 30.19272127508924 31.054435527384854 19.528337289035964 38-39 19.036954009384704 29.602194405380196 31.690638967404166 19.670212617830938 40-41 18.826564001169608 29.05770060847408 32.43626338434119 19.67947200601512 42-43 19.593283114496163 28.894721452540413 31.69634776312675 19.81564766983667 44-45 19.049485512190365 29.683301076316855 31.671423996435482 19.595789415057297 46-47 20.04462607388017 28.816538798925073 30.65623303025662 20.482602096938134 48-49 19.270318439410183 29.151338782216406 30.907698519890282 20.670644258483133 50-51 18.992327935504534 28.87926593241343 31.40617385371559 20.722232278366448 52-53 19.11987078628218 29.436917807265488 32.27648672356897 19.16672468288336 54-55 19.425012879600107 29.407956111892396 30.590233782146786 20.576797226360714 56-57 20.00048733622022 30.17209930519779 29.539676130271936 20.287737228310057 58-59 18.560643507488976 30.538618664446265 31.231263636730723 19.669474191334036 60-61 20.092456252110377 31.61537363674022 29.305568609431514 18.98660150171789 62-63 18.652828817186933 32.23071963594183 29.809142394033366 19.307309152837867 64-65 18.899263200326942 32.27068389462992 29.096520548307613 19.733532356735527 66-67 19.087045062920996 31.866116651871966 29.2869649974762 19.75987328773084 68-69 18.80912597295905 31.53327206650236 29.554980018658533 20.102621941880056 70-71 18.985435608961417 30.986716607025993 30.133808602180483 19.894039181832106 72-73 19.208513630651296 30.71583982287638 29.708866972780474 20.36677957369185 74-75 19.091750266831255 30.60368265426258 29.366476803956832 20.93809027494933 76-77 18.978999635187936 30.93139862262559 29.72772766640025 20.361874075786226 78-79 19.2799661927196 30.432330787808493 29.74281821555205 20.544884803919864 80-81 19.258153006377587 30.49085136602891 29.38584398585234 20.865151641741164 82-83 19.380339901645897 30.846467753625962 29.301251393396228 20.471940951331916 84-85 19.31036451345874 29.963664920809858 29.461838419942733 21.264132145788675 86-87 18.76291458965719 30.152538360856614 30.00675318165362 21.077793867832575 88-89 19.513066216645225 29.784401855781496 29.597194606426175 21.105337321147108 90-91 19.152383074116877 30.108884835489214 29.79009732800512 20.94863476238878 92-93 19.36388699369248 30.030284465113688 29.580542753310407 21.02528578788343 94-95 19.352051685487126 29.43768362132583 29.71483869171111 21.49542600147593 96-97 19.485164093067294 29.216989933026078 29.735724529720546 21.562121444186076 98-99 19.141800916192096 29.613124660605134 29.56369484398279 21.681379579219985 100-101 18.917840749835175 29.75523698993782 29.56517440852594 21.76174785170106 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1102.0 1 650.5 2 213.5 3 209.0 4 161.5 5 150.5 6 219.5 7 406.5 8 618.5 9 675.0 10 510.0 11 329.0 12 311.0 13 330.0 14 333.5 15 364.0 16 405.0 17 428.0 18 472.5 19 514.0 20 586.5 21 662.0 22 748.5 23 900.5 24 1042.0 25 1214.5 26 1567.0 27 2400.0 28 3761.0 29 5545.0 30 8696.0 31 13494.5 32 21516.0 33 30786.0 34 39625.0 35 47062.5 36 52520.0 37 55731.0 38 52287.5 39 46610.0 40 40806.0 41 34343.5 42 29486.5 43 26060.0 44 23489.0 45 21052.0 46 18717.5 47 16694.5 48 15359.0 49 13884.0 50 12138.5 51 10585.0 52 9300.0 53 8122.0 54 7147.5 55 6250.5 56 5317.5 57 4539.0 58 3935.5 59 3379.5 60 2791.0 61 2331.0 62 1842.0 63 1354.5 64 991.5 65 741.0 66 602.5 67 500.5 68 397.0 69 305.0 70 238.0 71 177.5 72 144.0 73 127.5 74 110.5 75 83.0 76 60.0 77 50.0 78 39.5 79 27.0 80 17.0 81 13.5 82 12.0 83 7.0 84 4.5 85 2.5 86 1.0 87 1.0 88 0.5 89 0.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020746599089377465 2 0.0 3 0.001113911360503488 4 0.019632687728873974 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.9619460031468E-5 12-13 0.0 14-15 6.9619460031468E-5 16-17 0.001531628120692296 18-19 6.9619460031468E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.39238920062936E-4 28-29 0.0049429816622342275 30-31 2.0885838009440397E-4 32-33 4.1771676018880795E-4 34-35 0.01148721090519222 36-37 0.003968309221793676 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.39238920062936E-4 60-61 0.0017404865007867 62-63 0.008772051963964968 64-65 0.002297442181038444 66-67 0.0038290703017307393 68-69 0.0027847784012587197 70-71 0.0 72-73 0.00661384870298946 74-75 0.005499937342485972 76-77 0.0016708670407552318 78-79 2.0885838009440397E-4 80-81 0.0069619460031468 82-83 0.009816343864436987 84-85 0.0018797254208496358 86-87 0.0013923892006293599 88-89 2.78477840125872E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.65814060346148E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 718190.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.294813741029536 #Duplication Level Percentage of deduplicated Percentage of total 1 62.1167822898139 21.302834788212497 2 17.18471854241151 11.786934432080383 3 7.588765900432682 7.8076593923884525 4 3.961166176914272 5.433898249381639 5 2.245582207161644 3.850591176738929 6 1.437074198792293 2.957051518777256 7 0.9687605588561262 2.325642404793857 8 0.6815970517619897 1.870019514928984 9 0.5196364994842011 1.603875326656614 >10 2.8368414160881708 17.928929137743673 >50 0.26642754852500095 6.3515379077581535 >100 0.1803509538162291 11.387898025662317 >500 0.00901754769081145 2.0607636529922666 >1k 0.0028692197198036434 2.05935794790837 >5k 4.0988853140052045E-4 1.273006523976669 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 9056 1.2609476600899483 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 4024 0.5602974143332544 RNA PCR Primer, Index 46 (95% over 21bp) ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 2424 0.33751514223255685 RNA PCR Primer, Index 46 (95% over 23bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2265 0.31537615394255003 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2166 0.30159150085631936 No Hit TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG 1660 0.23113660730447375 RNA PCR Primer, Index 46 (96% over 28bp) GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1082 0.15065651150809675 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1029 0.14327684874476115 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 963 0.13408708002060735 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 925 0.1287960010582158 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 904 0.12587198373689412 RNA PCR Primer, Index 46 (96% over 26bp) ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 873 0.12155557721494313 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 850 0.11835308205349558 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 808 0.1125050474108523 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 801 0.11153037497041174 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 761 0.10596081816789428 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.39238920062936E-4 0.0 18-19 0.0 0.0 0.0 2.08858380094404E-4 0.0 20-21 0.0 0.0 0.0 2.78477840125872E-4 0.0 22-23 0.0 0.0 0.0 0.0011835308205349559 0.0 24-25 0.0 0.0 0.0 0.002854397861290188 0.0 26-27 0.0 0.0 0.0 0.005708795722580375 0.0 28-29 0.0 0.0 0.0 0.010651777384814603 0.0 30-31 0.0 0.0 0.0 0.02394909425082499 0.0 32-33 0.0 0.0 0.0 0.05499937342485972 0.0 34-35 0.0 0.0 0.0 0.10561272086773695 0.0 36-37 0.0 0.0 0.0 0.1769030479399602 0.0 38-39 0.0 0.0 0.0 0.2513958701736309 0.0 40-41 0.0 0.0 0.0 0.3328506384104485 0.0 42-43 0.0 0.0 0.0 0.4237736532115457 0.0 44-45 0.0 0.0 0.0 0.5512468845291636 0.0 46-47 0.0 0.0 0.0 0.6921566716328548 0.0 48-49 0.0 0.0 0.0 0.8234589732522035 0.0 50-51 0.0 0.0 0.0 0.9466854175079018 0.0 52-53 0.0 0.0 0.0 1.0849496651303971 0.0 54-55 0.0 0.0 0.0 1.2401314415405396 0.0 56-57 0.0 0.0 0.0 1.4203066041019787 0.0 58-59 0.0 0.0 0.0 1.5852351049165263 0.0 60-61 0.0 0.0 0.0 1.7538534371127419 0.0 62-63 0.0 0.0 0.0 1.936882997535471 0.0 64-65 0.0 0.0 0.0 2.1727537281220846 0.0 66-67 0.0 0.0 0.0 2.447541736866289 0.0 68-69 0.0 0.0 0.0 2.7245575683315 0.0 70-71 0.0 0.0 0.0 3.0084657263398267 0.0 72-73 0.0 0.0 0.0 3.3013547946922124 0.0 74-75 0.0 0.0 0.0 3.625433381138696 0.0 76-77 0.0 0.0 0.0 4.005555632910511 0.0 78-79 0.0 0.0 0.0 4.395076511786574 0.0 80-81 0.0 0.0 0.0 4.795179548587422 0.0 82-83 0.0 0.0 0.0 5.203636920592044 0.0 84-85 0.0 0.0 0.0 5.6482267923529985 0.0 86-87 0.0 0.0 0.0 6.148442612679096 0.0 88-89 0.0 0.0 0.0 6.706860301591501 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1880 0.0 52.829006 1 GTATCAA 3245 0.0 38.075237 1 TTCGATA 90 2.4102255E-5 31.669523 4 CCGTGCT 60 0.0039509227 31.665113 9 CATTCCG 120 1.505141E-7 31.665113 9 TCAACGC 4000 0.0 30.5215 4 ATCAACG 4045 0.0 30.181953 3 CAACGCA 4110 0.0 29.700489 5 TATCAAC 4230 0.0 28.86194 2 AACGCAG 4295 0.0 28.642363 6 CGGAGAT 135 4.2052307E-7 28.16049 1 GTGGTAT 970 0.0 27.92461 1 TCTCGCA 35 0.008394267 27.141523 38-39 CTCGCAT 35 0.008394267 27.141523 38-39 CGCAGAG 4845 0.0 25.096807 8 ACGCAGA 4890 0.0 24.962988 7 GTGTCGC 50 0.0016503001 23.75876 30-31 CTTCGAT 100 0.001652176 23.752142 3 ATAGTGC 120 1.7105145E-4 23.748835 6 CTATAAC 510 0.0 23.286413 3 >>END_MODULE